scholarly journals Proteomic analysis of extracellular matrix from the hepatic stellate cell line LX-2 identifies CYR61 and Wnt-5a as novel constituents of fibrotic liver

2012 ◽  
Vol 11 (8) ◽  
pp. 4052-4064 ◽  
Author(s):  
S. Tamir Rashid ◽  
Jonathan D. Humphries ◽  
Adam Byron ◽  
Ameet Dhar ◽  
Janet A. Askari ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Proteomic Analysis ◽  
Stellate Cell ◽  
Fibrotic Liver

Activity and expression of ecto-5′-nucleotidase/CD73 are increased during phenotype conversion of a hepatic stellate cell line

Life Sciences ◽  
2008 ◽  
Vol 82 (1-2) ◽  
pp. 21-29 ◽  
Author(s):  
Cláudia M.B. Andrade ◽  
Gislaine C. Roesch ◽  
Márcia R. Wink ◽  
Eduardo L.M. Guimarães ◽  
Luiz F. Souza ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Stellate Cell

Stiffness is associated with hepatic stellate cell heterogeneity during liver fibrosis

2021 ◽  
Author(s):  
Enis Kostallari ◽  
Bo Wei ◽  
Delphine Sicard ◽  
Jiahui Li ◽  
Shawna A. Cooper ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Focal Adhesion ◽  
Hepatic Stellate Cell ◽  
Stellate Cell ◽  
Liver Stiffness ◽  
Specific Protein ◽  
Cellular Level ◽  
Fibrotic Liver ◽  
Soft Matrix

The fibrogenic wound-healing response in liver increases stiffness. Stiffness mechano-transduction in turn amplifies fibrogenesis. Here, we aimed to understand the distribution of stiffness in fibrotic liver, how it impacts hepatic stellate cell (HSC) heterogeneity and identify mechanisms by which stiffness amplifies fibrogenic responses. Magnetic resonance elastography and atomic force microscopy demonstrated a heterogenous distribution of liver stiffness at macroscopic and microscopic levels, respectively, in a carbon tetrachloride (CCl4) mouse model of liver fibrosis as compared to controls. High stiffness was mainly attributed to extracellular matrix dense areas. To identify a stiffness-sensitive HSC sub-population, we performed scRNA-seq on primary HSCs derived from healthy versus CCl4-treated mice. A sub-cluster of HSCs was matrix-associated with the most upregulated pathway in this sub-population being focal adhesion signaling, including a specific protein termed four and a half LIM domains protein 2 (FHL2). In vitro, FHL2 expression was increased in primary human HSCs cultured on stiff matrix as compared to HSCs on soft matrix. Moreover, FHL2 knockdown inhibited fibronectin and collagen 1 expression, whereas its overexpression promoted matrix production. In summary, we demonstrate stiffness heterogeneity at the whole organ, lobular, and cellular level which drives an amplification loop of fibrogenesis through specific focal adhesion molecular pathways.

Establishment of an Immortalized Human Hepatic Stellate Cell Line to Develop Antifibrotic Therapies

2003 ◽  
Vol 12 (5) ◽  
pp. 499-507 ◽  
Author(s):  
Norikuni Shibata ◽  
Takamasa Watanabe ◽  
Teru Okitsu ◽  
Masakiyo Sakaguchi ◽  
Michihiko Takesue ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Type I Collagen ◽  
Replicative Senescence ◽  
Stellate Cell ◽  
Smooth Muscle Actin ◽  
Population Doubling ◽  
Type I ◽  
Human Hepatic Stellate Cell ◽  
Ifn Γ

Because human hepatic stellate cells (HSCs) perform a crucial role in the progress of hepatic fibrosis, it is of great value to establish an immortalized human cell line that exhibits HSC characteristics and grows well in tissue cultures for the development of antifibrotic therapies. Thus, we engineered an immortalized human hepatic stellate cell (HSC) line TWNT-4 by retrovirally inducing human telomerase reverse transcriptase (hTERT) into LI 90 cells established from a human liver mesenchymal tumor. Parental LI 90 entered replicative senescence, whereas TWNT-4 showed telomerase activity and proliferated for more than population doubling level (PDL) 200 without any crisis. TWNT-4 expressed platelet-derived growth factor-β receptor (PDGF-βR), α-smooth muscle actin (α-SMA), and type I collagen (α1) and was considered to be an activated form of HSCs. Treatment of TWNT-4 cells with either 100 U/ml of IFN-γ or 1 ng/ml of rapamycin (Rapa) for 14 days led to lower expression of type I collagen (α1) at RNA and protein levels. Exposure of TWNT-4 cells to both of IFN-γ (10 U/ml) and Rapa (0.1 ng/ml) for 14 days effectively decreased the expression of type I collagen (α1), PDGF-βR, and α-SMA expression and suppressed TGF-β1 secretion of TWNT-4 cells. We successfully induced apoptosis by transducing TNF-related apoptosis-inducing ligand (TRAIL) into TWNT-4 cells using adenovirus vectors Ad/GT-TRAIL and Ad/PGK-GV-17. These findings suggested that immortalized activated HSC line TWNT-4 would be a useful means to develop antifibrotic therapies.

Relationship between oxidative stress levels and activation state on a hepatic stellate cell line

2006 ◽  
Vol 26 (4) ◽  
pp. 477-485 ◽  
Author(s):  
E. L. M. Guimaraes ◽  
M. F. S. Franceschi ◽  
I. Grivicich ◽  
F. Dal-pizzol ◽  
J. C. F. Moreira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Stellate Cell ◽  
Stress Levels

PAV-1, a new rat hepatic stellate cell line converts retinol into retinoic acid, a process altered by ethanol

2002 ◽  
Vol 34 (8) ◽  
pp. 1017-1029 ◽  
Author(s):  
Patrick Sauvant ◽  
Vincent Sapin ◽  
Armand Abergel ◽  
Carsten K Schmidt ◽  
Loı̈c Blanchon ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Stellate Cell
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