scholarly journals Auxin Type, Auxin Concentration, and Air and Substrate Temperature Difference Play Key Roles in the Rooting of Juvenile Hardwood Pecan Cuttings

2015 ◽  
Vol 25 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Ji-Yu Zhang ◽  
Zhong-Ren Guo ◽  
Rui Zhang ◽  
Yong-Rong Li ◽  
Lin Cao ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Substrate Temperature ◽  
Adventitious Roots ◽  
Carya Illinoinensis ◽  
Naphthalene Acetic Acid ◽  
Ambient Temperatures ◽  
Auxin Concentration ◽  
Rooting Of Cuttings ◽  
Bottom Heat ◽  
Indole 3 Acetic Acid

This study examined the ability to vegetatively propagate 1-year-old pecan (Carya illinoinensis) through the rooting of hardwood cuttings. Cuttings were treated with varying concentrations of different auxins and different combinations of media and ambient temperatures. Under different temperature conditions, all auxin treatments induced the rooting of cuttings but did not promote sprouting. The effectiveness of the induction of adventitious roots was as follows: 1-naphthalene acetic acid (NAA) > indole 3-butyric acid > indole 3-acetic acid. The base of the parent shoot treated by NAA at a concentration of 0.09%, planted in substrate with bottom heat was the most effective, with 82% rooting, 8.3 roots/cutting and root lengths of 7.3 cm. These findings suggested that auxin and substrate/air temperature differences are both indispensable in the process of adventitious roots formation in pecan. This study revealed that the propagation of hardwood cuttings derived from branches of 1-year-old pecan is possible.

scholarly journals Vegetative propagation of native fruit species of páramo Thibaudia floribunda and Cavendishia bracteata by cuttings

2017 ◽  
Vol 35 (1) ◽  
pp. 12-22
Author(s):  
Lina María López C. ◽  
Mariam Vásquez M. ◽  
Héctor Lancheros R. ◽  
Stanislav Magnitskiy
Keyword(s):  
Acetic Acid ◽  
Adventitious Roots ◽  
Naphthalene Acetic Acid ◽  
Fruit Species ◽  
Wild Fruit ◽  
The Andes ◽  
Shoot Production ◽  
Parenchyma Cells ◽  
Rooting Of Cuttings ◽  
Andes Region

Cavendishia bracteata and Thibaudia floribunda (Ericaceae) are wild fruit shrubs native to the Andes region. This study aimed to characterize the rooting potential of basal and apical cuttings using naphthalene acetic acid (NAA) in different concentrations (0, 200, 500, or 1,000 mg L-1). In C. bracteata, the cutting position on the branch affected its sprouting and rooting, with apical cuttings presenting the best performance with auxin application. The rooting of cuttings was more successful in C. bracteata than in T. floribunda. NAA applications between 200 to 500 mg L-1 were more suitable for the propagation of C. bracteata due to the higher percentages of rooting and shoot production. For propagation of T. floribunda, the use of apical cuttings without the application of auxins is recommended. Adventitious roots in cuttings of both species were originated from parenchyma cells of the stem.

scholarly journals Role of Growth Regulators on In Vitro Callus Induction and Direct Regeneration in Physalis minima Linn

2015 ◽  
Vol 44 ◽  
pp. 38-44 ◽  
Author(s):  
H. Sandhya ◽  
Rao Srinath
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Basal Medium ◽  
Direct Regeneration ◽  
Naphthalene Acetic Acid ◽  
Stem Explants ◽  
Dichlorophenoxy Acetic Acid ◽  
Indole 3 Acetic Acid

Suitable protocol for induction of callus and regeneration was developed from different explants viz., node, stem and leaves in Physalis minima. MS basal medium supplemented with various concentrations (1.0-4.0mg/l) of auxins like 2,4-Dichlorophenoxy acetic acid (2,4-D), α-naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinins (0.5-1.5mg/l) like BAP or Kn were used. All the three explants responded for induction of callus, however stem explants were found superior, followed by node and leaf. Callus induction was observed in all the auxins and combination of growth regulators used with varied mass (2010±1.10) and highest percentage of callus induction was observed from stem at 2.0mg/l 2,4-D (90%) followed by NAA (70%) and IAA (50%). Organogenesis was induced when nodal explants were transferred on MS medium supplemented with 2,4-D and Kn at various concentrations, maximum being on 2.0mg/l 2,4-D + 1.0mg/l Kn (90%). Regenerated shoots were elongated on 0.5mg/l GA3. The shoots were subsequently rooted on MS + 1.0mg/l IBA (95%) medium. Rooted shoots were hardened and acclimatized, later they were transferred to polycups containing soil, cocopeat and sand in the ratio 1:2:1.Keywords:Physalis minima, Node, Stem, Leaf, callus and growth regulators.

scholarly journals Influence of phytohormones on vegetative propagation of different forms of pannonian thyme (Thymus pannonicus All.)

2020 ◽  
Vol 26 (2) ◽  
pp. 39-52
Author(s):  
Vladimir Filipović ◽  
Vladan Ugrenović ◽  
Zoran Maksimović ◽  
Vera Popović ◽  
Danica Paunović ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Root Hairs ◽  
Morphological Properties ◽  
Naphthalene Acetic Acid ◽  
Plant Establishment ◽  
Rooted Cuttings ◽  
Average Percentage ◽  
Plant Collection ◽  
Rooting Of Cuttings ◽  
The Impact

The paper examined the impact of the application of three different phytohormones (INCIT 2, INCIT 5 and INCIT 8, all based on a-Naphthalene Acetic Acid (NAA)), with the cuttings of two forms of Pannonian thyme (the L-16 form, with hairy leaves, and the L-9 form, with hairless leaves), established during two periods (March and May), on the percentage of the rooted cuttings and the morphological properties of the seedlings. As the control, the variant without the application of the phytohormones was taken. The research was conducted in the period from 2019 to 2020, in a plastic greenhouse, using the plant collection of the Institute for Medicinal Plants Research "Dr Josif Pančić", which is located in Pančevo city, Serbia (44°52'20"N; 20°42'06"E; 74 m.a.s.l.). For this research was used the Pannonian thyme species (Thymus pannonicus All.) from the Lamiaceae family, which is grown and produced at the Institute. Plant establishment was carried out in four repetitions with 33 cuttings per each variant. Standard measures of care were used during the period of production of Pannonian thyme. The measurement was performed after 60 days from the date of conducted the experiment. The following parameters were measured: the percentage of rooted cuttings (%), the mass of the rooted plantlings (g), the mass of roots (g), the length of rooting (cm) and the number of root hairs of the formed rootings. The measuring was done using a ruler, millimetre paper and an analytical balance. Given the results achieved, INCIT 2 proved to be the most suitable phytohormone for the rooting of Pannonic thyme cuttings, with the average percentage of rooted cuttings of 61.3%. The lowest rooting percentage was recorded in the control variant, only 29.4%. Satisfactory rooting was found in the cuttings treated with INCIT 8 (57.6%). The L-16 form cuttings showed a higher rooting rate, an average of 53.8%, whereas the L-9 form cuttings had a lower average percentage of rooted cuttings (45.0%) for both plant establishment periods. Greater success and quality in the rooting of cuttings was recorded in the second (May) period of plant establishment, averaging 58.1%, which was higher by 17.4% than the percentage of rooted cuttings in the first establishment period (40.7%). It is important to note that the rooting of the L-9 form was significantly higher in the second establishment period (58.1%) than that in the first establishment period (31.9%).

scholarly journals In vitro callus induction of gerbera from leaf explant

2020 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Sadia Afrin Jui ◽  
Md. Mijanur Rahman Rajib ◽  
M. Mofazzal Hossain ◽  
Sharmila Rani Mallik ◽  
Iffat Jahan Nur ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Leaf Explants ◽  
Leaf Explant ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Indole 3 Acetic Acid

The experiment was designed to evaluate the effect of growth regulators on leaf explant of Gerbera for callus induction. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA) were used to initiate cultures. These were added to Murashige and Skoog medium in different combinations and concentrations. Leaf explants cultured on MS medium supplemented with BAP+ 2, 4-D+ IAA in T4 treatment & BAP+ 2,4-D in T5 treatment showed the best results for callus induction. On the other hand callus was induced early in the combination of BA+ 2,4-D + IAA hormone in T5, T9 & T8 treatment respectively. The rate of callus induction was very low in BA + NAA combinations but it was much earlier.   

scholarly journals Determining the Rooting Ability of Hardwood Cuttings of Blueberry (Vaccinium corymbosum L.) cv. ‘Bluecrop’ Stored in Refrigerating Conditions

2020 ◽  
Vol 8 (3) ◽  
pp. 81
Author(s):  
Sabri Braha ◽  
Petrit Rama ◽  
Agim Zajmi
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Adventitious Roots ◽  
Vaccinium Corymbosum ◽  
Mother Plant ◽  
Winter Period ◽  
Naphthalene Acetic Acid ◽  
Intact Control ◽  
Rooting Ability ◽  
Time Periods

This experiment has been carried out in order to determine the rooting ability of hardwood cuttings of the blueberry cultivar ‘Bluecrop’ collected during the deep dormant winter period in the last part of January. In order to induce the adventitious roots formation, the cuttings were stimulated using Indol Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA) in various concentrations (1500, 3000, 4500 ppm), whilst one row in each box remained intact (control).  The collection of cuttings off the mother plant at the beginning of January enable the nutrients and other matter that help the process of rooting not to move from the base of cutting towards the top and consequently they remain at the base of cutting which results with quite a high rooting percentage (up to 72.5%) compared to the cuttings collected in February with rooting percentage of 67.5% and in March (52%). In both types of substrate, turf and turf-perlite 2:1 and in different time periods when cuttings have been collected, IBA proved to be better in inducing adventitious roots compared to NAA.

Plant Regeneration from Cultured Medicago truncatula with Particular Reference to Abscisic Acid and Light Treatments

1998 ◽  
Vol 46 (1) ◽  
pp. 151 ◽  
Author(s):  
K. E. Nolan ◽  
R. J. Rose
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Plant Regeneration ◽  
Medicago Truncatula ◽  
Callus Induction ◽  
Naphthalene Acetic Acid ◽  
Marked Inhibition ◽  
Embryo Formation ◽  
Auxin Concentration

Medicago truncatula (Jemalong 2HA) can be regenerated by somatic embryogenesis utilising 1-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). There is a requirement for both NAA and BAP for callus induction and embryo formation. There is no requirement for a drop in auxin concentration to induce embryos. Abscisic acid (ABA) when present with NAA and BAP during embryo formation at a concentration of 1 µM, increases the number of embryos per callus. The ABA treatment stimulates embryo numbers in both light and darkness. The conversion efficiency of embryo to plant is unchanged irrespective of the presence of ABA during embryo formation, indicating that ABA does not improve the regeneration of the embryos once formed. Importantly, the presence of light in the embryo formation period causes a marked inhibition of embryo conversion.

scholarly journals Micropropagation, Characterization, and Conservation of Phytophthora cinnamomi-Tolerant Holm Oak Mature Trees

Forests ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1634
Author(s):  
Mª Teresa Martínez ◽  
Isabel Arrillaga ◽  
Ester Sales ◽  
María Amparo Pérez-Oliver ◽  
Mª del Carmen González-Mas ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Phytophthora Cinnamomi ◽  
Expression Profiles ◽  
Holm Oak ◽  
Naphthalene Acetic Acid ◽  
Shoot Cultures ◽  
Mature Trees ◽  
Phenylpropanoid Biosynthesis ◽  
Indole 3 Acetic Acid

Holm oak populations have deteriorated drastically due to oak decline syndrome. The first objective of the present study was to investigate the use of axillary budding and somatic embryogenesis (SE) to propagate asymptomatic holm oak genotypes identified in disease hotspots in Spain. Axillary budding was achieved in two out of six tolerant genotypes from the south-western region and in two out of four genotypes from the Mediterranean region. Rooting of shoots cultured on medium supplemented with 3 mg L−1 of indole-3-acetic acid plus 0.1 mg L−1 α-naphthalene acetic acid was achieved, with rates ranging from 8 to 36%. Shoot cultures remained viable after cold storage for 9–12 months; this procedure is therefore suitable for medium-term conservation of holm oak germplasm. SE was induced in two out of the three genotypes tested, by using nodes and shoot tips cultured in medium without plant growth regulators. In vitro cloned progenies of the tolerant genotypes PL-T2 and VA5 inhibited growth of Phytophthora cinnamomi mycelia when exposed to the oomycete in vitro. Significant differences in total phenol contents and in the expression profiles of genes regulating phenylpropanoid biosynthesis were observed between in vitro cultured shoots derived from tolerant trees and cultures established from control genotypes.

scholarly journals Naphthalene Acetic Acid and 2,3,5-triiodobenzoic Acid Affect the Response of Mature Orange Fruit to Abscission Chemicals

HortScience ◽  
2002 ◽  
Vol 37 (2) ◽  
pp. 348-352 ◽  
Author(s):  
Rongcai Yuan ◽  
Ulrich Hartmond ◽  
Walter J. Kender
Keyword(s):  
Acetic Acid ◽  
Abscission Zone ◽  
Naphthalene Acetic Acid ◽  
Mature Fruit ◽  
Fruit Abscission ◽  
Triiodobenzoic Acid ◽  
Valencia Orange ◽  
Auxin Concentration ◽  
Auxin Transport Inhibitor ◽  
Orange Fruit

Effects of NAA, TIBA, ethephon, and CMN-Pyrazole on fruit detachment force (FDF) of mature `Valencia' and `Hamlin' orange [Citrus sinensis (L.) Osb.] fruit were examined in 2000 and 2001. NAA effectively inhibited the reduction in FDF or fruit abscission caused by ethephon when applied to the abscission zone 24 hours before ethephon application, but had no significant effect when applied to the fruit without contacting the abscission zone, or to the peduncle ≈4 cm above the abscission zone. TIBA, an auxin transport inhibitor, decreased FDF of mature fruit and promoted fruit abscission when applied alone as a spray to the canopy or directly to the fruit peduncle. This response was dependent on TIBA concentration. TIBA was more effective when applied in combination with ethephon or CMN-Pyrazole than alone. These results are consistent with our previous data that endogenous auxin concentration in the abscission zone of mature `Valencia' orange fruit is one of the factors controlling the sensitivity and thus the responsiveness of the abscission zone of mature fruit to abscission chemicals. Chemical names used: 5-chloro-3-methyl-4-nitro-pyrazole (CMN-Pyrazole); 2-chloroethylphosphonic acid (ethephon); naphthalene acetic acid (NAA); 2,3,5-triiodobenzoic acid (TIBA).

Effect of Exogenous Indole-3-acetic Acid and Naphthalene Acetic Acid on Regeneration of Damask Rose Cuttings in Three Growing Media

2007 ◽  
Vol 10 (20) ◽  
pp. 3626-3631
Author(s):  
Rahmat Ullah Khan . ◽  
Muhammad Sohail Khan . ◽  
Abdur Rashid . ◽  
Muhammad Arshad Faro .
Keyword(s):  
Acetic Acid ◽  
Naphthalene Acetic Acid ◽  
Growing Media ◽  
Damask Rose ◽  
Indole 3 Acetic Acid

scholarly journals Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plant

2011 ◽  
Vol 77 (4) ◽  
pp. 275-280 ◽  
Author(s):  
Arzu Ucar Turker ◽  
Esra Canserver Multu
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Plant Growth ◽  
Medicinal Plant ◽  
Skin Diseases ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Solanum Dulcamara ◽  
Petiole Explants ◽  
Indole 3 Acetic Acid

<em>Solanum dulcamara</em> L. (bittersweet) is a medicinal plant that has been used to treat skin diseases, warts, tumors, felons, arthritis, rheumatism, bronchial congestion, heart ailments, ulcerative colitis, eye inflammations, jaundice and pneumonia. A reliable in vitro culture protocol for bittersweet was established. Explants (leaf and petiole segments) were cultured on Murashige and Skoog minimal organics (MSMO) medium with various plant growth regulator combinations. Leaf explants formed more shoots than petiole explants. Plant regeneration was observed through indirect organogenesis with both explants. Best shoot proliferation was obtained from leaf explants with 3 mg/l BA (benzyladenine) and 0.5 mg/l IAA (indole-3-acetic acid). Regenerated shoots were transferred to rooting media containing different levels of IAA (indole-3-acetic acid), IBA (indole-3-butyric acid), NAA (naphthalene acetic acid) or 2,4-D (2,4 dichlorophenoxyacetic acid). Most shoots developed roots on medium with 0.5 mg/l IBA. Rooted explants were transferred to vermiculate in Magenta containers for acclimatization and after 2 weeks, they were planted in plastic pots containing potting soil and maintained in the plant growth room.

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