scholarly journals Influence of phytohormones on vegetative propagation of different forms of pannonian thyme (Thymus pannonicus All.)

2020 ◽  
Vol 26 (2) ◽  
pp. 39-52
Author(s):  
Vladimir Filipović ◽  
Vladan Ugrenović ◽  
Zoran Maksimović ◽  
Vera Popović ◽  
Danica Paunović ◽  
...  

The paper examined the impact of the application of three different phytohormones (INCIT 2, INCIT 5 and INCIT 8, all based on a-Naphthalene Acetic Acid (NAA)), with the cuttings of two forms of Pannonian thyme (the L-16 form, with hairy leaves, and the L-9 form, with hairless leaves), established during two periods (March and May), on the percentage of the rooted cuttings and the morphological properties of the seedlings. As the control, the variant without the application of the phytohormones was taken. The research was conducted in the period from 2019 to 2020, in a plastic greenhouse, using the plant collection of the Institute for Medicinal Plants Research "Dr Josif Pančić", which is located in Pančevo city, Serbia (44°52'20"N; 20°42'06"E; 74 m.a.s.l.). For this research was used the Pannonian thyme species (Thymus pannonicus All.) from the Lamiaceae family, which is grown and produced at the Institute. Plant establishment was carried out in four repetitions with 33 cuttings per each variant. Standard measures of care were used during the period of production of Pannonian thyme. The measurement was performed after 60 days from the date of conducted the experiment. The following parameters were measured: the percentage of rooted cuttings (%), the mass of the rooted plantlings (g), the mass of roots (g), the length of rooting (cm) and the number of root hairs of the formed rootings. The measuring was done using a ruler, millimetre paper and an analytical balance. Given the results achieved, INCIT 2 proved to be the most suitable phytohormone for the rooting of Pannonic thyme cuttings, with the average percentage of rooted cuttings of 61.3%. The lowest rooting percentage was recorded in the control variant, only 29.4%. Satisfactory rooting was found in the cuttings treated with INCIT 8 (57.6%). The L-16 form cuttings showed a higher rooting rate, an average of 53.8%, whereas the L-9 form cuttings had a lower average percentage of rooted cuttings (45.0%) for both plant establishment periods. Greater success and quality in the rooting of cuttings was recorded in the second (May) period of plant establishment, averaging 58.1%, which was higher by 17.4% than the percentage of rooted cuttings in the first establishment period (40.7%). It is important to note that the rooting of the L-9 form was significantly higher in the second establishment period (58.1%) than that in the first establishment period (31.9%).

2017 ◽  
Vol 35 (1) ◽  
pp. 12-22
Author(s):  
Lina María López C. ◽  
Mariam Vásquez M. ◽  
Héctor Lancheros R. ◽  
Stanislav Magnitskiy

Cavendishia bracteata and Thibaudia floribunda (Ericaceae) are wild fruit shrubs native to the Andes region. This study aimed to characterize the rooting potential of basal and apical cuttings using naphthalene acetic acid (NAA) in different concentrations (0, 200, 500, or 1,000 mg L-1). In C. bracteata, the cutting position on the branch affected its sprouting and rooting, with apical cuttings presenting the best performance with auxin application. The rooting of cuttings was more successful in C. bracteata than in T. floribunda. NAA applications between 200 to 500 mg L-1 were more suitable for the propagation of C. bracteata due to the higher percentages of rooting and shoot production. For propagation of T. floribunda, the use of apical cuttings without the application of auxins is recommended. Adventitious roots in cuttings of both species were originated from parenchyma cells of the stem.


2020 ◽  
Vol 8 (3) ◽  
pp. 1880-1885
Author(s):  
Tran Quyen Nguyen ◽  
Ba Huy Nguyen ◽  
Dieu Hien Tran Thi ◽  
Oanh Duong Thi ◽  
Quang Ngoc Nguyen ◽  
...  

HortScience ◽  
2009 ◽  
Vol 44 (2) ◽  
pp. 495-498 ◽  
Author(s):  
Giancarlo Fascella ◽  
Giovanvito Zizzo

A simple and efficient in vivo propagation protocol of two cultivars of Euphorbia ×lomi Rauh is described. Mother plants of ‘Nguen Muang’ and ‘Porn Ying Yai’ were soilless grown in 1 coconut coir dust:1 perlite (by volume) and 2 sphagnum peat:1 perlite (by volume) to produce cuttings. Plants cultured in peat-based substrate showed thicker stems (36.0 versus 30.5 mm, respectively), higher production of cuttings (10.4 versus 5.6 per plant), higher water absorption (265.7 versus 153.5 mL/plant/d), and higher content of nutrients in the root zone as compared with those cultivated in coir dust mixture. Cuttings harvested from 1-year-old mother plants of both cultivars were treated with 4000 ppm of α-naphthalene acetic acid (NAA) powder and inserted into bottom-heated benches filled with different substrates [perlite, 1 perlite:1 coir dust (by volume), coir dust] for rooting. Cuttings in pure perlite evidenced higher rooting percentage (86.2%) and longer roots (6.2 cm) than those in other media. Application of NAA resulted in higher rooting capacity (78.8 versus 68.3%, respectively) and higher amount of roots per cutting (13.4 versus 10.2) than treatment without auxin. Rooted cuttings grown in 1 peat:1 perlite medium (by volume) produced higher marketable potted plants than in 2 peat:1 perlite medium.


Author(s):  
Muhammad Nisar ◽  
Habib Ur Rahman ◽  
Muhammad Sohail Khan ◽  
Ilham Khan ◽  
Saba Fatima ◽  
...  

This study assessed the impact of naphthalene acetic Acid (NAA) on the growth and yield of Okra cv. Sabz Pari at Agricultural Research Institute, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan. A field experiment was set out in randomised complete block design with NAA application as a main factor. Different NAA concentrations were obtained by dissolving NAA in distilled water at the rate of 0 (control), 10, 30, 50, 70, 90 and 110 mg/L. NAA treatments along with control were foliar applied to okra plants after 30 days of sowing. Lower concentration of NAA (30-50 mg/L) stimulated maximum increase in plant height (59.5-57.2%), number of leaves/plant (50.4-45.5%,), total plant leaf area (113.9-119.4%), internodes/plant (72.5-61.6%), internode length (53.2-44.5%), pod diameter (53.5-49.9%), pod fresh weight (126.8-111.8%), pod yield (271.2-255.8%) compared to control (0 mg NAA/L). NAA at higher concentrations (>50 mg/L) had a supressing effect on most parameters. It was inferred that yield production in okra is influenced by multiple yield-determining component traits and their mutual interactions which could be manipulated by the application of NAA. Foliar application of NAA (30-50 mg/L) have a beneficial impact on plant characters and yield of Okra, hence recommended in Okra cultivation.    


2015 ◽  
Vol 25 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Ji-Yu Zhang ◽  
Zhong-Ren Guo ◽  
Rui Zhang ◽  
Yong-Rong Li ◽  
Lin Cao ◽  
...  

This study examined the ability to vegetatively propagate 1-year-old pecan (Carya illinoinensis) through the rooting of hardwood cuttings. Cuttings were treated with varying concentrations of different auxins and different combinations of media and ambient temperatures. Under different temperature conditions, all auxin treatments induced the rooting of cuttings but did not promote sprouting. The effectiveness of the induction of adventitious roots was as follows: 1-naphthalene acetic acid (NAA) > indole 3-butyric acid > indole 3-acetic acid. The base of the parent shoot treated by NAA at a concentration of 0.09%, planted in substrate with bottom heat was the most effective, with 82% rooting, 8.3 roots/cutting and root lengths of 7.3 cm. These findings suggested that auxin and substrate/air temperature differences are both indispensable in the process of adventitious roots formation in pecan. This study revealed that the propagation of hardwood cuttings derived from branches of 1-year-old pecan is possible.


2020 ◽  
Vol 38 ◽  
Author(s):  
Z. HASNAIN ◽  
I. BAKHSH ◽  
I. HUSSAIN ◽  
SHEHERYAR ◽  
KHAN E.A.

ABSTRACT: The study aimed to highlight the impact of naphthalene acetic acid (NAA) and irrigation regimes on the productivity of coarse rice under agro-ecological conditions of Dera Ismail Khan, Pakistan. The experiment was comprised of two factors with four levels (0, 60, 90, 120 mL ha-1) of NAA and irrigation at the depths (60, 75, 90 and 105 cm) of soil profile. The data was interpreted to observe the plant height (cm), productive tillers (m-2), sterility percentage (%), biological yield (t ha-1), and grain yield (t ha-1) of coarse rice. The benefit cost ratio (BCR) was also calculated. The results indicated that the application of NAA was found very effective in improving paddy yield and hence resulted in better BCR value. The maximum paddy yield was attained under the application of NAA 90 mL ha-1 as PGR (NAA) at 75 cm irrigation depth. The experimental data confirm that the application of NAA at the rate of 90 mL ha-1 with irrigation upto depth of 75 cm increases grain yield in rice and ensures better economic returns, thus inclusion of 90 mL ha-1 as PGR (NAA) at 75 cm irrigation depth under arid conditions assist in boosting paddy yield.


2020 ◽  
Vol 11 (4) ◽  
pp. 5059-5066
Author(s):  
Sushma B K ◽  
Raveesha H R

The present work is aimed to determine the chemical constituents in Baliospermum montanum methanolic extracts. An in vitro regenerated procedure was developed for the induction of callus from stem explant cultured on Murashige and Skoog (MS) medium fortified with various concentration and permutations of 2, 4-dichloro phenoxy acetic acid, 1-naphthalene acetic acid, 6-benzyl amino purine and gibberellic acid. FTIR & GC-MS analysis was done according to standard procedure. The quantitative estimation of β-sitosterol was done by HPLC method. Maximum fresh and dry weight of callus was estimated in the combination of GA3 (0.5 mg/L) + NAA (2 mg/L) compared to other concentration. The FTIR analysis showed various functional compounds with different characteristic peak values in the extracts. Major bioactive constituents were recognized in the GC-MS analysis. Root extract revealed the existence of 1-hexadecanol, pentanoic acid, 2-(aminooxy)- and 1-hexacosanol. Leaf extract showed the presence of propanoic acid, 2-oxo-, trimethylsilyl ester, 9,12-octadecadienoic acid (z,z)-, trimethylsilyl ester, docosane, 1,22-dibromo- and pentatriacontane. Stem and stem derived callus exhibit the presence of 1,6,3,4-dihydro-2-deoxy-beta-d-lyxo-hexopyranose, n-hexadecanoic acid and pentanoic acid, 2-(aminooxy). The methanolic extract of leaf exhibited 0.2149 % of β-sitosterol content. There were no peaks observed in the root, stem and stem derived callus. Further studies are necessary for the isolation and characterization of bioactive compounds from B. montanum.


2013 ◽  
Vol 14 (3) ◽  
Author(s):  
Daru Mulyono

The objective of this research is to know the optimal formula of Indole Butiric Acid (IBA), Naphthalene Acetic Acid (NAA), Vitamine B1 and the combination with shading intensities to the acclimatization of Gaharu stump (Aquilaria beccariana). This research used Factorial Design with basic analysis of Complete Randomized Design in order to know theeffect of treatment. The research was carried out in Agroindustry and Biotechnology Laboratory, Ciampea, Bogor, from July to September 2007. The results of the research showed that after 8 weeks of treatment: (a). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 10 mg/l + Vitamine B1 1 mg/l was the best formula for increasingheight of Gaharu stump 4.660 cm. (b). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 30 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sum of Gaharu leaf stump 12.337 leafs, (c). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sumof Gaharu root stump 3.783 roots, and (d). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing length of Gaharu root stump 3.686 cm.


Author(s):  
А. М. Grebennikov ◽  
А. S. Frid ◽  
V. P. Belobrov ◽  
V. А. Isaev ◽  
V. М. Garmashоv ◽  
...  

The article assesses the relationships between the morphological properties of agrochernozems and yield of peas on the plots, experience with different methods of basic treatment (moldboard plowing at the depth of 20 - 22, 25 - 27 and 14 - 16 cm, moldboard plowing to a depth of 14 - 16 cm, combined midwater moldboard, mid-water subsurface, surface to a depth of 6 - 8 cm and zero tillage) is inherent in V.V. Dokuchaev Research Institute of Agriculture of the Central Black Earth strip, in the fall of 2014. The research was conducted in 2015 - 2016, with the application of mineral fertilizers (N60Р60К60) and unfertilized background. The highest pea yields in the fertilized as the background, and without the use of fertilizers was observed in dumping plowing and especially in the variant with deep moldboard plowing, which creates in comparison with other ways of handling the best conditions for the growth and development of peas. The lowest yield of pea was obtained with zero processing. Apparently legalistic migrational-mizelial agrochernozems the Central Chernozem zone of minimum tillage in the cultivation of peas are not effective, what is evident already in the first year after the laying of experience with different basic treatments. As shown by the results of applying multifactor analysis of variance studied the mapping properties of the soil can have the same significant impact on the yield of agricultural crops, as options for the field experiments aimed at assessing the impact of various treatments on yield.


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