scholarly journals Dependence property of isoelectric points and pH environment on enzyme immobilization on maghemite/hydroxyapatite composite particles

2022 ◽  
Vol 130 (1) ◽  
pp. 74-80
Author(s):  
Takeshi YABUTSUKA ◽  
Masaya YAMAMOTO ◽  
Shigeomi TAKAI
Keyword(s):  
Enzyme Immobilization ◽  
Composite Particles ◽  
Hydroxyapatite Composite ◽  
Isoelectric Points ◽  
Dependence Property

Magnetite/Hydroxyapatite Composite Particles-Assisted Pore Alignment of Tilapia Fish Scale Collagen-Based Scaffold

2016 ◽  
Vol 696 ◽  
pp. 121-128 ◽  
Author(s):  
Vincent Irawan ◽  
Tomomei Sugiyama ◽  
Toshiyuki Ikoma
Keyword(s):  
Magnetic Field ◽  
Magnetic Particles ◽  
Collagen Fiber ◽  
Magnetic Particle ◽  
Ice Crystals ◽  
Composite Particles ◽  
Fish Scale ◽  
Particle Movement ◽  
Hydroxyapatite Composite ◽  
The Magnetic Field

Pore alignment is a vital step to obtain bone-mimicking microstructure in collagen-based scaffold. The porous structure can be controlled by altering the configuration of ice crystals formed in collagen solution. In this study, movements of magnetite/hydroxyapatite composite particles were used to alter the configuration of pore alignment formed in 1.0 and 1.5wt% collagen solutions (HCol 1.0 and HCol 1.5 respectively) by applying moving external magnetic field. In case of HCol 1.0 pores were aligned parallel to the magnetic field, while for HCol 1.5 pores were aligned at curve shape. Different results of pore alignment were thought to originate from differences in collagen fiber alignment caused by magnetic particles movement paths. This result thus opens up the possibility of controlling pore alignment by utilizing magnetic particle movement.

scholarly journals Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo

2017 ◽  
Vol 18 (4) ◽  
pp. 858 ◽  
Author(s):  
You-Young Jo ◽  
Seong-Gon Kim ◽  
Kwang-Jun Kwon ◽  
HaeYong Kweon ◽  
Weon-Sik Chae ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Composite Particles ◽  
Engineering Applications ◽  
Hydroxyapatite Composite

The Hierarchic Order of Intermediate Filament Structure and Assembly

1985 ◽  
Vol 43 ◽  
pp. 722-725
Author(s):  
U. Aebi ◽  
E.C. Glavaris ◽  
R. Eichner
Keyword(s):  
Tissue Specificity ◽  
Structural Model ◽  
Eukaryotic Cells ◽  
Cdna Sequencing ◽  
Filament Structure ◽  
Keratin Filaments ◽  
Isoelectric Points ◽  
Immunological Crossreactivity

Five different classes of intermediate-sized filaments (IFs) have been identified in differentiated eukaryotic cells: vimentin in mesenchymal cells, desmin in muscle cells, neurofilaments in nerve cells, glial filaments in glial cells and keratin filaments in epithelial cells. Despite their tissue specificity, all IFs share several common attributes, including immunological crossreactivity, similar morphology (e.g. about 10 nm diameter - hence ‘10-nm filaments’) and the ability to reassemble in vitro from denatured subunits into filaments virtually indistinguishable from those observed in vivo. Further more, despite their proteinchemical heterogeneity (their MWs range from 40 kDa to 200 kDa and their isoelectric points from about 5 to 8), protein and cDNA sequencing of several IF polypeptides (for refs, see 1,2) have provided the framework for a common structural model of all IF subunits.

Sialic Acid Dependent Polypeptide Chain Heterogeneity of Human Fibrinogen Demonstrated by Two-Dimensional Electrophoresis

1982 ◽  
Vol 47 (01) ◽  
pp. 019-021 ◽  
Author(s):  
Cemal Kuyas ◽  
André Haeberli ◽  
P Werner Straub
Keyword(s):  
Sialic Acid ◽  
Polypeptide Chain ◽  
Two Dimensional ◽  
Charge Heterogeneity ◽  
Human Fibrinogen ◽  
Two Dimensional Electrophoresis ◽  
Isoelectric Points ◽  
Polypeptide Chains ◽  
Dimensional Electrophoresis ◽  
Chain Type

SummaryHuman fibrinogen was compared with asialofibrinogen by two-dimensional electrophoresis to evaluate the contribution of sialic acid to the heterogeneity of the γ- and Bβ-polypeptide chains.Reduced fibrinogen showed three major variants for both the γ- and Bβ-chains. In addition two minor γ-bands with a more acidic isoelectric point than the normal γ-chains were observed. Electrophoresis in the second dimension (SDS) suggests that these most acidic bands are γ-chain-variants with a higher molecular weight. In asialofibrinogen only two predominant variants with more alkaline isoelectric points were present in each chain type.It is concluded that enzymatic removal of sialic acid partially reduces the heterogeneity of the γ- and Bβ-polypeptide chains of human fibrinogen, but additional sources producing charge heterogeneity must be sought.

Environmentally Friendly Preparation of Magnetic Composites Featuring Proteolytic Properties

INEOS OPEN ◽  
2020 ◽  
Author(s):  
N. A. Samoilova ◽  
Keyword(s):  
Enzyme Immobilization ◽  
Maleic Acid ◽  
Environmentally Friendly ◽  
Synthetic Polymer ◽  
Magnetic Composites ◽  
Interpolyelectrolyte Complex ◽  
Hydrolysis Rates ◽  
Poly Ethylene ◽  
Noncovalent Binding

The enzyme-containing magnetic composites are presented. The magnetic matrix for enzyme immobilization is obtained by sequential application of an amine-containing polysaccharide—chitosan and a synthetic polymer—poly(ethylene-alt-maleic acid) to the magnetite microparticles to form the interpolyelectrolyte complex shell. Then, the enzyme (trypsin) is immobilized by covalent or noncovalent binding. Thus, the suggested composites can be readily obtained in the environmentally friendly manner. The enzyme capacity of the resulting composites reaches 28.0–32.6 mg/g. The maximum hydrolysis rates of the H-Val-Leu-Lys-pNA substrate provided by these composites range within 0.60·10–7–0.77·10–7 M/min.

Coulomb interaction of composite particles

1983 ◽  
Vol 141 (11) ◽  
pp. 552
Author(s):  
D.A. Kirzhnits ◽  
F.M. Pen'kov
Keyword(s):  
Coulomb Interaction ◽  
Composite Particles

Biocatalytic Ketone Reductions Using Biobeads

2020 ◽  
Author(s):  
Jia Shen Chew ◽  
Ken Chi Lik Lee ◽  
THI THANH NHA HO
Keyword(s):  
Enzyme Immobilization ◽  
High Throughput ◽  
High Throughput Screening ◽  
Chiral Hplc ◽  
Micro Scale

<p>Lee and coworkers offers a kind of new concept to enzyme immobilization and explores its suitability in the context of miniaturisation and high-throughput screening. Here, polystyrene-immobilized ketoreductases are compared with its non-immobilized counterparts in terms of conversion and stereoselectivity (both determined by chiral HPLC), and the study indicates that the BioBeads perform similarly (sometimes slightly more selective) which may be useful whenever defined micro-scale amounts of biocatalysts were required in high-throughput experiment settings.</p>

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