In Vivo Imaging of Fibroblast Activity Using a 68Ga-Labeled Fibroblast Activation Protein Alpha (FAP-α) Inhibitor

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Xueying Zhang ◽  
Daoyun Chen ◽  
John W. Babich ◽  
Samuel J.E. Green ◽  
Xiang-Hua Deng ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fibroblast Activation Protein ◽  
Fibroblast Activation ◽  
Fibroblast Activation Protein Alpha

scholarly journals Activatable Near-Infrared Fluorescent Probe for In Vivo Imaging of Fibroblast Activation Protein-alpha

2012 ◽  
Vol 23 (8) ◽  
pp. 1704-1711 ◽  
Author(s):  
Jinbo Li ◽  
Kai Chen ◽  
Hongguang Liu ◽  
Kai Cheng ◽  
Meng Yang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fibroblast Activation Protein ◽  
Fibroblast Activation ◽  
Fibroblast Activation Protein Alpha

A novel fibroblast activation protein-targeted near-infrared fluorescent off–on probe for cancer cell detection, in vitro and in vivo imaging

2018 ◽  
Vol 6 (10) ◽  
pp. 1449-1451 ◽  
Author(s):  
Jie Xing ◽  
Qiuyu Gong ◽  
Ruifen Zou ◽  
Zihou Li ◽  
Yuanzhi Xia ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fibroblast Activation Protein ◽  
Cell Detection ◽  
Fibroblast Activation ◽  
Design And Synthesis ◽  
Cancer Cell Detection

Design and synthesis of a novel fibroblast activation protein “off–on” near-infrared fluorescent probe for cell detection, in vitro and in vivo imaging.

Feasibility of in vivo Imaging of Fibroblast Activation Protein in Human Arterial Walls

2021 ◽  
pp. jnumed.121.262863
Author(s):  
Meiqi Wu ◽  
Jing Ning ◽  
Jingle Li ◽  
Zhichao Lai ◽  
Ximin Shi ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fibroblast Activation Protein ◽  
Arterial Walls ◽  
Fibroblast Activation

An improved production and purification protocol for recombinant soluble human fibroblast activation protein alpha

2021 ◽  
Vol 181 ◽  
pp. 105833
Author(s):  
Cecy R. Xi ◽  
Arianna Di Fazio ◽  
Naveed Ahmed Nadvi ◽  
Michelle Sui Wen Xiang ◽  
Hui Emma Zhang ◽  
...  
Keyword(s):  
Human Fibroblast ◽  
Fibroblast Activation Protein ◽  
Fibroblast Activation ◽  
Fibroblast Activation Protein Alpha ◽  
Purification Protocol

scholarly journals Fibroblast activation protein targeted near infrared photoimmunotherapy (NIR PIT) overcomes therapeutic resistance in human esophageal cancer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ryoichi Katsube ◽  
Kazuhiro Noma ◽  
Toshiaki Ohara ◽  
Noriyuki Nishiwaki ◽  
Teruki Kobayashi ◽  
...  
Keyword(s):  
Esophageal Cancer ◽  
Cancer Cells ◽  
Cancer Progression ◽  
Near Infrared ◽  
Fibroblast Activation Protein ◽  
Therapeutic Resistance ◽  
Fibroblast Activation ◽  
Human Esophageal Cancer

AbstractCancer-associated fibroblasts (CAFs) have an important role in the tumor microenvironment. CAFs have the multifunctionality which strongly support cancer progression and the acquisition of therapeutic resistance by cancer cells. Near-infrared photoimmunotherapy (NIR-PIT) is a novel cancer treatment that uses a highly selective monoclonal antibody (mAb)-photosensitizer conjugate. We developed fibroblast activation protein (FAP)-targeted NIR-PIT, in which IR700 was conjugated to a FAP-specific antibody to target CAFs (CAFs-targeted NIR-PIT: CAFs-PIT). Thus, we hypothesized that the control of CAFs could overcome the resistance to conventional chemotherapy in esophageal cancer (EC). In this study, we evaluated whether EC cell acquisition of stronger malignant characteristics and refractoriness to chemoradiotherapy are mediated by CAFs. Next, we assessed whether the resistance could be rescued by eliminating CAF stimulation by CAFs-PIT in vitro and in vivo. Cancer cells acquired chemoradiotherapy resistance via CAF stimulation in vitro and 5-fluorouracil (FU) resistance in CAF-coinoculated tumor models in vivo. CAF stimulation promoted the migration/invasion of cancer cells and a stem-like phenotype in vitro, which were rescued by elimination of CAF stimulation. CAFs-PIT had a highly selective effect on CAFs in vitro. Finally, CAF elimination by CAFs-PIT in vivo demonstrated that the combination of 5-FU and NIR-PIT succeeded in producing 70.9% tumor reduction, while 5-FU alone achieved only 13.3% reduction, suggesting the recovery of 5-FU sensitivity in CAF-rich tumors. In conclusion, CAFs-PIT could overcome therapeutic resistance via CAF elimination. The combined use of novel targeted CAFs-PIT with conventional anticancer treatments can be expected to provide a more effective and sensible treatment strategy.

Role of Alpha-Smooth Muscle Actin and Fibroblast Activation Protein Alpha in Ovarian Neoplasms

2018 ◽  
Vol 83 (4) ◽  
pp. 381-387 ◽  
Author(s):  
Ana Carolinne da Silva ◽  
Millena Prata Jammal ◽  
Renata Margarida Etchebehere ◽  
Eddie Fernando Candido Murta ◽  
Rosekeila Simões Nomelini
Keyword(s):  
Smooth Muscle ◽  
Ovarian Neoplasms ◽  
Smooth Muscle Actin ◽  
Fibroblast Activation Protein ◽  
Muscle Actin ◽  
Alpha Smooth Muscle Actin ◽  
Fibroblast Activation ◽  
Fibroblast Activation Protein Alpha
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