scholarly journals Xeno-Transplantation of macro-encapsulated islets and Pluripotent Stem Cell-Derived Pancreatic Progenitors without Immunosuppression

2017 ◽  
Vol 02 (01) ◽  
Author(s):  
Michael A. Bukys
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Pancreatic Progenitors

scholarly journals Towards consistent generation of pancreatic lineage progenitors from human pluripotent stem cells

2015 ◽  
Vol 370 (1680) ◽  
pp. 20140365 ◽  
Author(s):  
Maria Rostovskaya ◽  
Nicholas Bredenkamp ◽  
Austin Smith
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cells ◽  
Type I ◽  
Pancreatic Progenitors ◽  
Stem Cell Lines

Human pluripotent stem cells can in principle be used as a source of any differentiated cell type for disease modelling, drug screening, toxicology testing or cell replacement therapy. Type I diabetes is considered a major target for stem cell applications due to the shortage of primary human beta cells. Several protocols have been reported for generating pancreatic progenitors by in vitro differentiation of human pluripotent stem cells. Here we first assessed one of these protocols on a panel of pluripotent stem cell lines for capacity to engender glucose sensitive insulin-producing cells after engraftment in immunocompromised mice. We observed variable outcomes with only one cell line showing a low level of glucose response. We, therefore, undertook a systematic comparison of different methods for inducing definitive endoderm and subsequently pancreatic differentiation. Of several protocols tested, we identified a combined approach that robustly generated pancreatic progenitors in vitro from both embryo-derived and induced pluripotent stem cells. These findings suggest that, although there are intrinsic differences in lineage specification propensity between pluripotent stem cell lines, optimal differentiation procedures may consistently direct a substantial fraction of cells into pancreatic specification.

scholarly journals Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Haisong Liu ◽  
Ronghui Li ◽  
Hsin-Kai Liao ◽  
Zheying Min ◽  
Chao Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
High Efficiency ◽  
Human Pluripotent Stem Cell ◽  
Β Cells ◽  
Cell Replacement Therapy ◽  
Pancreatic Progenitor ◽  
Pancreatic Progenitors ◽  
Cell Source ◽  
Different Sources

AbstractHuman pluripotent stem cell (hPSC)-derived pancreatic β cells are an attractive cell source for treating diabetes. However, current derivation methods remain inefficient, heterogeneous, and cell line dependent. To address these issues, we first devised a strategy to efficiently cluster hPSC-derived pancreatic progenitors into 3D structures. Through a systematic study, we discovered 10 chemicals that not only retain the pancreatic progenitors in 3D clusters but also enhance their potentiality towards NKX6.1+/INS+ β cells. We further systematically screened signaling pathway modulators in the three steps from pancreatic progenitors toward β cells. The implementation of all these strategies and chemical combinations resulted in generating β cells from different sources of hPSCs with high efficiency. The derived β cells are functional and can reverse hyperglycemia in mice within two weeks. Our protocol provides a robust platform for studying human β cells and developing hPSC-derived β cells for cell replacement therapy.

scholarly journals Efficient Generation of NKX6-1+ Pancreatic Progenitors from Multiple Human Pluripotent Stem Cell Lines

2015 ◽  
Vol 4 (4) ◽  
pp. 591-604 ◽  
Author(s):  
M. Cristina Nostro ◽  
Farida Sarangi ◽  
Chaoxing Yang ◽  
Andrew Holland ◽  
Andrew G. Elefanty ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Pancreatic Progenitors ◽  
Efficient Generation ◽  
Stem Cell Lines

scholarly journals Dynamic proteome profiling of human pluripotent stem cell-derived pancreatic progenitors

Stem Cells ◽  
2020 ◽  
Vol 38 (4) ◽  
pp. 542-555 ◽  
Author(s):  
Larry Sai Weng Loo ◽  
Heidrun Vethe ◽  
Andreas Alvin Purnomo Soetedjo ◽  
Joao A. Paulo ◽  
Joanita Jasmen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Proteome Profiling ◽  
Pancreatic Progenitors

Pluripotent stem cell colonies provide a developmental landscape for pharmacogenomic drug discovery

Planta Medica ◽  
2015 ◽  
Vol 81 (11) ◽  
Author(s):  
DJ Hoeppner ◽  
J Chenoweth ◽  
SK Kim ◽  
A Jaishankar ◽  
Y Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Drug Discovery ◽  
Pluripotent Stem Cell

Regenerative potential of human pluripotent stem cell-derived MSCs in a Gunn rat liver injury model

2015 ◽  
Vol 53 (12) ◽  
Author(s):  
LS Spitzhorn ◽  
M Megges ◽  
C Kordes ◽  
I Sawitza ◽  
S Götze ◽  
...  
Keyword(s):  
Stem Cell ◽  
Liver Injury ◽  
Rat Liver ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Gunn Rat ◽  
Injury Model
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