scholarly journals Dynamic proteome profiling of human pluripotent stem cell-derived pancreatic progenitors

Stem Cells ◽  
2020 ◽  
Vol 38 (4) ◽  
pp. 542-555 ◽  
Author(s):  
Larry Sai Weng Loo ◽  
Heidrun Vethe ◽  
Andreas Alvin Purnomo Soetedjo ◽  
Joao A. Paulo ◽  
Joanita Jasmen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Proteome Profiling ◽  
Pancreatic Progenitors

scholarly journals Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Haisong Liu ◽  
Ronghui Li ◽  
Hsin-Kai Liao ◽  
Zheying Min ◽  
Chao Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
High Efficiency ◽  
Human Pluripotent Stem Cell ◽  
Β Cells ◽  
Cell Replacement Therapy ◽  
Pancreatic Progenitor ◽  
Pancreatic Progenitors ◽  
Cell Source ◽  
Different Sources

AbstractHuman pluripotent stem cell (hPSC)-derived pancreatic β cells are an attractive cell source for treating diabetes. However, current derivation methods remain inefficient, heterogeneous, and cell line dependent. To address these issues, we first devised a strategy to efficiently cluster hPSC-derived pancreatic progenitors into 3D structures. Through a systematic study, we discovered 10 chemicals that not only retain the pancreatic progenitors in 3D clusters but also enhance their potentiality towards NKX6.1+/INS+ β cells. We further systematically screened signaling pathway modulators in the three steps from pancreatic progenitors toward β cells. The implementation of all these strategies and chemical combinations resulted in generating β cells from different sources of hPSCs with high efficiency. The derived β cells are functional and can reverse hyperglycemia in mice within two weeks. Our protocol provides a robust platform for studying human β cells and developing hPSC-derived β cells for cell replacement therapy.

scholarly journals Efficient Generation of NKX6-1+ Pancreatic Progenitors from Multiple Human Pluripotent Stem Cell Lines

2015 ◽  
Vol 4 (4) ◽  
pp. 591-604 ◽  
Author(s):  
M. Cristina Nostro ◽  
Farida Sarangi ◽  
Chaoxing Yang ◽  
Andrew Holland ◽  
Andrew G. Elefanty ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Pancreatic Progenitors ◽  
Efficient Generation ◽  
Stem Cell Lines

Regenerative potential of human pluripotent stem cell-derived MSCs in a Gunn rat liver injury model

2015 ◽  
Vol 53 (12) ◽  
Author(s):  
LS Spitzhorn ◽  
M Megges ◽  
C Kordes ◽  
I Sawitza ◽  
S Götze ◽  
...  
Keyword(s):  
Stem Cell ◽  
Liver Injury ◽  
Rat Liver ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Gunn Rat ◽  
Injury Model

Generation of expandable human pluripotent stem cell-derived hepatocyte-like liver organoids

2019 ◽  
Vol 71 (5) ◽  
pp. 970-985 ◽  
Author(s):  
Seon Ju Mun ◽  
Jae-Sung Ryu ◽  
Mi-Ok Lee ◽  
Ye Seul Son ◽  
Soo Jin Oh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Liver Organoids
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