scholarly journals Efficient Generation of NKX6-1+ Pancreatic Progenitors from Multiple Human Pluripotent Stem Cell Lines

2015 ◽  
Vol 4 (4) ◽  
pp. 591-604 ◽  
Author(s):  
M. Cristina Nostro ◽  
Farida Sarangi ◽  
Chaoxing Yang ◽  
Andrew Holland ◽  
Andrew G. Elefanty ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Pancreatic Progenitors ◽  
Efficient Generation ◽  
Stem Cell Lines

scholarly journals Brief Report: Efficient Generation of Hematopoietic Precursors and Progenitors from Human Pluripotent Stem Cell Lines

Stem Cells ◽  
2011 ◽  
Vol 29 (7) ◽  
pp. 1158-1164 ◽  
Author(s):  
Niels-Bjarne Woods ◽  
Aaron S. Parker ◽  
Roksana Moraghebi ◽  
Margaret K. Lutz ◽  
Amy L. Firth ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Efficient Generation ◽  
Stem Cell Lines

scholarly journals Generation of human pluripotent stem cell lines with suppressed expression of the Notch ligand DLL4 using short hairpin RNAs

2016 ◽  
Vol 16 (3) ◽  
pp. 735-739
Author(s):  
Federico González-Pozas ◽  
Rosa Montes ◽  
Lourdes López-Onieva ◽  
Tamara Romero ◽  
Joan Domingo-Reinés ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Notch Ligand ◽  
Short Hairpin ◽  
Stem Cell Lines ◽  
Short Hairpin Rnas

scholarly journals Towards consistent generation of pancreatic lineage progenitors from human pluripotent stem cells

2015 ◽  
Vol 370 (1680) ◽  
pp. 20140365 ◽  
Author(s):  
Maria Rostovskaya ◽  
Nicholas Bredenkamp ◽  
Austin Smith
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cells ◽  
Type I ◽  
Pancreatic Progenitors ◽  
Stem Cell Lines

Human pluripotent stem cells can in principle be used as a source of any differentiated cell type for disease modelling, drug screening, toxicology testing or cell replacement therapy. Type I diabetes is considered a major target for stem cell applications due to the shortage of primary human beta cells. Several protocols have been reported for generating pancreatic progenitors by in vitro differentiation of human pluripotent stem cells. Here we first assessed one of these protocols on a panel of pluripotent stem cell lines for capacity to engender glucose sensitive insulin-producing cells after engraftment in immunocompromised mice. We observed variable outcomes with only one cell line showing a low level of glucose response. We, therefore, undertook a systematic comparison of different methods for inducing definitive endoderm and subsequently pancreatic differentiation. Of several protocols tested, we identified a combined approach that robustly generated pancreatic progenitors in vitro from both embryo-derived and induced pluripotent stem cells. These findings suggest that, although there are intrinsic differences in lineage specification propensity between pluripotent stem cell lines, optimal differentiation procedures may consistently direct a substantial fraction of cells into pancreatic specification.

scholarly journals Nodal/Activin Signaling Predicts Human Pluripotent Stem Cell Lines Prone to Differentiate Toward the Hematopoietic Lineage

2010 ◽  
Vol 18 (12) ◽  
pp. 2173-2181 ◽  
Author(s):  
Veronica Ramos-Mejia ◽  
Gustavo J Melen ◽  
Laura Sanchez ◽  
Ivan Gutierrez-Aranda ◽  
Gertrudis Ligero ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Activin Signaling ◽  
Hematopoietic Lineage ◽  
Stem Cell Lines

Hypoxic culture of human pluripotent stem cell lines is permissible using mouse embryonic fibroblasts

2012 ◽  
Vol 7 (5) ◽  
pp. 675-683 ◽  
Author(s):  
Jennifer L Badger ◽  
Meg L Byrne ◽  
Farlan S Veraitch ◽  
Chris Mason ◽  
Ivan B Wall ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Stem Cell Lines

scholarly journals Generation of human pluripotent stem cell lines (iPSCs) from mesenchymal stem cells (MSCs) from three elderly patients with osteoarthritis

2020 ◽  
Vol 44 ◽  
pp. 101721 ◽  
Author(s):  
Lydiane Pichard ◽  
Jean-Marc Brondelo ◽  
Fabienne Becker ◽  
Romain Desprat ◽  
Frédéric De Ceuninck ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Elderly Patients ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Stem Cell Lines

scholarly journals Expression of miRNAs from the Imprinted DLK1/DIO3 Locus Signals the Osteogenic Potential of Human Pluripotent Stem Cells

Cells ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 1523 ◽  
Author(s):  
Laetitia Barrault ◽  
Jacqueline Gide ◽  
Tingting Qing ◽  
Lea Lesueur ◽  
Jorg Tost ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Osteogenic Differentiation ◽  
Cell Lines ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cells ◽  
Osteogenic Potential ◽  
Human Pluripotent Stem Cell ◽  
Stem Cell Lines

Substantial variations in differentiation properties have been reported among human pluripotent cell lines (hPSC), which could affect their utility and clinical safety. We characterized the variable osteogenic capacity observed between different human pluripotent stem cell lines. By focusing on the miRNA expression profile, we demonstrated that the osteogenic differentiation propensity of human pluripotent stem cell lines could be associated with the methylation status and the expression of miRNAs from the imprinted DLK1/DIO3 locus. More specifically, quantitative analysis of the expression of six different miRNAs of that locus prospectively identified human embryonic stem cells and human-induced pluripotent stem cells with differential osteogenic differentiation capacities. At the molecular and functional levels, we showed that these miRNAs modulated the expression of the activin receptor type 2B and the downstream signal transduction, which impacted osteogenesis. In conclusion, miRNAs of the imprinted DLK1/DIO3 locus appear to have both a predictive value and a functional impact in determining the osteogenic fate of human pluripotent stem cells.

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