Effects of vegetable oil and yeast fermented cassava pulp (YFCP) supplementation on feed intake, nutrient digestibility and rumen fermentation in Thai Friesian dairy cows

Author(s):  
N. Suphrap ◽  
C. Wachirapakorn ◽  
C. Thamrongyoswittayakul and C. Wongnen

This study was conducted to investigate the effect of vegetable oil and yeast fermented cassava pulp (YFCP) supplementation on feed intake, nutrient digestibility and rumen fermentation in Thai Friesian dairy cows (Thai native x Holstein Friesian). Eight Thai Friesian dairy cows (447±44 kg.BW) were assigned to 4×4 double latin square design (DLSD) with two sources of oil i.e. palm oil (PO) or soybean oil (SBO) and four levels of YFCP (0, 5, 10 and 20%DM) in the dietary treatments. All cows received total mixed ration (TMR) comprised of rice straw to concentrate at a ratio of 40:60. The results showed that supplementation of SBO had lowered feed intake, nutrients digestibility, metabolize energy intake (MEI), total digestible nutrient (TDN) and methane emission than PO treatment. However, cows received SBO had greater total volatile fatty acid (TVFA), propionic acid (C3), butyric acid (C4) than cows received on PO (P less than 0.05). In addition, supplementation of YFCP at 10%DM in the diet as an optimum level in dairy cow diets (P greater than 0.05). Finally, the interaction between the addition of SBO and YFCP at 10%DM (SBO+YFCP) had a positive effect on enhancing ether extract intake (EEI) in dairy cows.

Author(s):  
N. Suphrap ◽  
C. Wachirapakorn ◽  
C. Thamrongyoswittayakul ◽  
C. Wongnen

The investigation aimed to study the effect of vegetable oil sources on feed intake, nutrient digestibility and biohydrogenation bacterial population in Thai Friesian dairy cows. Three fistula Thai Friesian cows with mean body weight (BW) of 600±100 kg were assigned to receive three vegetable oil sources i.e. palm oil (PO), soybean oil (SBO) and sunflower oil (SFO) included at 4 %DM in commercial concentrate. All cows were fed on different diets that contained concentrate of 1 %BW and rice straw of 1 %BW according to a 3×3 latin square design (LSD). The results revealed that dry matter digestibility (DMD) and organic matter digestibility (OMD) tended to be higher in cows fed on SBO and SFO (P=0.06). Moreover, the DNA copy numbers (copies/ml) of biohydrogenation bacteria (Ruminococcus albus) and cellulolytic bacteria (Ruminococcus flavefaciens and Prevotella ruminicola) were higher in cows fed on SBO (P less than 0.05). In summary, supplementation of SBO in diet resulted in a higher nutrient digestibility and rumen biohydrogenation bacteria population.


2017 ◽  
Vol 57 (2) ◽  
pp. 301 ◽  
Author(s):  
Pablo Gomes de Paiva ◽  
Elmeson Ferreira de Jesus ◽  
Tiago Antonio Del Valle ◽  
Gustavo Ferreira de Almeida ◽  
Artur Gabriel Brao Vilas Boas Costa ◽  
...  

Our objective was to evaluate the effects of providing increasing levels of chitosan on nutrient digestibility, ruminal fermentation, blood parameters, nitrogen utilisation, microbial protein synthesis, and milk yield and composition of lactating dairy cows. Eight rumen-fistulated Holstein cows [average days in lactation = 215 ± 60.9; and average bodyweight (BW) = 641 ± 41.1 kg] were assigned into a replicated 4 × 4 Latin square design, with 21-day evaluation periods. Cows were assigned to be provided with four levels of chitosan, placed into the rumen through the fistula, as follows: (1) Control: with no provision of chitosan; (2) 75 mg/kg BW; (3) 150 mg/kg BW; and (4) 225 mg/kg BW. Chitosan had no effect on dry matter intake (P > 0.73); however, chitosan increased (P = 0.05) crude protein digestibility. Propionate concentration was increased (P = 0.02), and butyrate, isobutyrate, isovalerate and acetate : propionate ratio were decreased (P ≤ 0.04) by chitosan. Chitosan had no effect (P > 0.25) on acetate, pH and NH3 ruminal concentration. Glucose, urea, and hepatic enzyme concentrations in the blood were similar (P > 0.30) among treatments. Nitrogen balance was not affected, but chitosan increased milk nitrogen (P = 0.02). Microbial protein synthesis was not affected by chitosan (P > 0.44). Chitosan increased (P = 0.02) milk yield, fat-corrected milk, protein and lactose production. Chitosan changes ruminal fermentation and improves milk yield of lactating dairy cows; therefore, we conclude that chitosan can be used as a rumen modulator instead of ionophores in diets for dairy cows.


Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1310
Author(s):  
Babak Darabighane ◽  
Ilma Tapio ◽  
Laura Ventto ◽  
Piia Kairenius ◽  
Tomasz Stefański ◽  
...  

Four multiparous dairy cows were used in a 4 × 4 Latin square to examine how starch level and oil mixture impact dry matter (DM) intake and digestibility, milk yield and composition, rumen fermentation, ruminal methane (CH4) emissions, and microbial diversity. Experimental treatments comprised high (HS) or low (LS) levels of starch containing 0 or 30 g of a mixture of sunflower and fish oils (2:1 w/w) per kg diet DM (LSO and HSO, respectively). Intake of DM did not differ between cows fed LS and HS diets while oil supplementation reduced DM intake. Dietary treatments did not affect milk and energy corrected milk yields. There was a tendency to have a lower milk fat concentration due to HSO compared with other treatments. Both high starch level and oil supplementation increased digestibility of gross energy. Cows receiving HS diets had higher levels of total rumen VFA while acetate was lower than LS without any differences in rumen pH, or ruminal CH4 emissions. Although dietary oil supplementation had no impact on rumen fermentation, decreased CH4 emissions (g/day and g/kg milk) were observed with a concomitant increase in Anoplodinium-Diplodinium sp. and Epidinium sp. but a decrease in Christensenellaceae, Ruminococcus sp., Methanobrevibacter ruminantium and Mbb. gottschalkii clades.


Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2597
Author(s):  
Juana C. Chagas ◽  
Mohammad Ramin ◽  
Ruth Gomez Exposito ◽  
Hauke Smidt ◽  
Sophie J. Krizsan

This study examined the effects of partly replacing grass silage (GS) with maize silage (MS), with or without rapeseed oil (RSO) supplementation, on methane (CH4) emissions, production performance, and rumen microbiome in the diets of lactating dairy cows. The effect of individual pre-trial CH4-emitting characteristics on dietary emissions mitigation was also examined. Twenty Nordic Red cows at 71 ± 37.2 (mean ± SD) days in milk were assigned to a replicated 4 × 4 Latin square design with four dietary treatments (GS, GS supplemented with RSO, GS plus MS, GS plus MS supplemented with RSO) applied in a 2 × 2 factorial arrangement. Partial replacement of GS with MS decreased the intake of dry matter (DM) and nutrients, milk production, yield of milk components, and general nutrient digestibility. Supplementation with RSO decreased the intake of DM and nutrients, energy-corrected milk yield, composition and yield of milk fat and protein, and general digestibility of nutrients, except for crude protein. Individual cow pre-trial measurements of CH4-emitting characteristics had a significant influence on gas emissions but did not alter the magnitude of CH4 emissions. Dietary RSO decreased daily CH4, yield, and intensity. It also increased the relative abundance of rumen Methanosphaera and Succinivibrionaceae and decreased that of Bifidobacteriaceae. There were no effects of dietary MS on CH4 emissions in this study, but supplementation with 41 g RSO/kg of DM reduced daily CH4 emissions from lactating dairy cows by 22.5%.


2020 ◽  
Vol 26 (1-2) ◽  
pp. 34-42
Author(s):  
SM Amanullah ◽  
MM Rahamn ◽  
MA Kabir ◽  
SMJ Hossain

A study was conducted to know effects of dietary oils and calcium salts (Ca-salts) of n-6 and n-3 fatty acid (FA) on feed intake, digestibility, enteric methane and blood metabolic profiles in bull. Four rumen cannulated bulls were used in 4x4 Latin Square Designs and supplied with Napier silage and concentrate mixture. Four dietary treatments were sunflower oil (SFO; n-6), linseed oil (LSO;n-3), Ca-salt of SFO (Ca-SFO; n-6) and Ca-salt of LSO (Ca-LSO; n-3). Oils and salts were mixed with concentrate part of the ration at 3% (w/w). Data were analyzed for ANOVA in 2x2 Factorial arrangements. It was found that, intake of DM (p<0.05), OM (P<0.01) and ADF (p<0.05) were reduced by feeding Ca-salts compared to oil sources. On the other hand, intake of CP (p<0.01) and NDF (p<0.05) was reduced by Ca-salts of n-3 FA only, but not of n-6 FA. The EE intake was affected by both FAs and their sources (p<0.01). Digestibility of DM was found higher (p<0.05) in n-3 FA and further Ca-salts reduced (p<0.05) DM digestibility. The CP (p<0.01) and ADF (p<0.01) digestibility was reduced by Ca-salts of either FA, while NDF digestibility was increased (p<0.01) by Ca-salt only in n-3 FA but not in n-6 FA. Concentrations of plasma glucose, urea nitrogen (BUN), cortisol and IgF-1 were affected neither by FA types nor by its sources (p>0.05). Concentrations of total cholesterol, IgG and insulin were decreased (p<0.01) by Ca-salt of FA, while triglyceride was decreased (p<0.05) by n-6 FA. The HDL was found to increase by n-3 FA (p<0.01) as well as Ca-salts (p<0.01) of both FA, but LDL was decreased by n-3 FA (p<0.01) as well as Ca-salts (p<0.01) of both FA. Methane production (% of total gas) were significantly reduced (p<0.01) by n-3 FA at 0 and 4 h after feeding, but not at 2h, while Ca-salts irrespective of FA reduced (p<0.05) methane in all different time periods. Oil sources compared to Ca-salts. In conclusion, Ca-salts of FAs hampered nutrient digestibility but helped to improve lipid profiles in plasma by reducing total cholesterol and LDL, but increasing HDL. Bang. J. Livs. Res. Vol. 26 (1&2), 2019: P. 34-42


1992 ◽  
Vol 1 (3) ◽  
pp. 279-290
Author(s):  
Pekka Huhtanen ◽  
Harri Miettinen

Twelve Finnish Ayrshire cows were used in a 4 x 4 Latin square design with 4-week experimental periods to study the effects of replacing increasing amount of barley with wet distiller’s solubles (WDS) on feed intake, milk production, digestibility and blood constituents. The four dietary treatments consisted of grass silage ad libitum and 7.8 kg dry matter (DM)/d of barley, of which 0 (WDSO), 1 (WDS1), 2 (WDS2) and 3 kg DM/d (WDS3) was replaced with WDS. Mainly because of a greater intake of concentrate with WDS containing diets silage DM intake varied quadratically (P


Author(s):  
Zachary T Traughber ◽  
Fei He ◽  
Jolene M Hoke ◽  
Gary M Davenport ◽  
Maria R C de Godoy

Abstract Ancient grains are becoming an increasingly abundant carbohydrate source in the pet food market as a result of their popularity and novelty in the human market. Thus, it is imperative to evaluate the characteristics of these ingredients in vivo. Ten adult intact female beagles were used in a replicated 5x5 Latin square design. Five dietary treatments were evaluated containing either: rice (CON), amaranth (AM), white proso millet (WPM), quinoa (QU), or oat groats (OG). All diets were formulated to include 40% of the test grain and to be isonitrogenous, isocaloric, and nutritionally complete and balanced for adult dogs at maintenance. The objectives were (1) to evaluate the effects of the novel carbohydrate sources on total apparent total tract digestibility (ATTD), fecal microbiota, and fermentative end-product concentrations and (2) to evaluate the effects of novel carbohydrate sources on the post-prandial glycemic and insulinemic responses in healthy adult dogs. All diets were well-accepted by the dogs and fecal scores remained within ideal range for all treatments. In terms of ATTD, all diets were well-digested by the dogs; WPM had the highest digestibility of dry and organic matter in contrast with dogs fed the other treatments (P &lt; 0.05). Additionally, ATTD of total dietary fiber was highest for WPM (72.6%) in contrast with QU (63.5%) and CON (50.8%) but did not differ from AM (65.7%) and OG (66.6%). Dogs fed AM or OG had greater (P &lt; 0.05) fecal concentrations of total short-chain fatty acids, as well as propionate and butyrate concentrations, than CON. Ancient grain inclusion appears to beneficially shift fecal microbial populations, with increases in relative abundances of butyrogenic bacteria (i.e., members of the Lachnospiraceae family) observed for OG and reductions in Fusobacteriaceae for both AM and OG when compared with CON. Post-prandial glycemic and insulinemic responses did not differ among treatments. Together, these data suggest that ancient grains can be included up to 40% of the diet while eliciting beneficial effects on overall host health without detrimentally affecting nutrient digestibility.


2015 ◽  
Vol 44 (2) ◽  
pp. 120-127 ◽  
Author(s):  
EK Ndelekwute ◽  
KD Afolabi ◽  
HO Uzegbu ◽  
UL Unah ◽  
KU Amaefule

An experiment was conducted to determine the dietary effect of different levels of ground black pepper (Piper nigrum) on growth performance and apparent nutrient digestibility of broiler chickens. There were five dietary treatments each containing 0, 0.25, 0.50, 0.75 and 1.00 % black pepper (BP) fed at both starter and finisher phases. One hundred and fifty (150) day old chicks of Arbor-acre strain were divided into five dietary groups having three replication with 10 in each. The five dietary treatments were randomly assigned into five groups. The birds were fed and watered ad libitum. At the starter phase 0.25 and 0.5 % BP improved live weight significantly (p<0.05). Feed intake was significantly (p<0.05) reduced at 1.00% BP. There were no significant differences (P>0.05) in daily gain, feed: gain ratio and protein efficiency ratio. At the finisher phase, final live weight was improved by 0.25% (p<0.05). Final live weight, feed intake, weight gain, feed: gain ratio and protein efficiency ratio were negatively affected by 0.75 and 1.0%BP. Fecal moisture was significantly (P<0.05) reduced by 0.25% while dry matter, protein and ether extract digestibility were improved by all the levels of BP. None of the BP levels improved crude fibre digestibility (p>0.05) over the control. NFE and energy utilization were improved by 0.25 and 0.5%, but significantly (p<0.05) reduced by 1.00% BP.Bang. J. Anim. Sci. 2015. 44(2): 120-127


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