Evaluation of lipid changes in buffalo (Bubalus bubalis) spermatozoa during in-vitro capacitation and acrosome reaction

2015 ◽  
Author(s):  
Ravi Kant ◽  
S. K. Atreja ◽  
S. S. Hasan
Keyword(s):  
Total Lipid ◽  
Lipid Content ◽  
Acrosome Reaction ◽  
Neutral Lipids ◽  
Total Lipid Content ◽  
Bubalus Bubalis ◽  
Total Lipids ◽  
Substantial Loss ◽  
One Step

In this study, buffalo spermatozoa were in-vitro capacitated and acrosome reacted using modified Tyrode’s bicarbonate-buffered medium (sp-TALP media). Lipids were extracted from freshly ejaculated, in-vitro capacitated and acrosome reacted buffalo spermatozoa. The total lipid content of freshly ejaculated spermatozoa (171.5±2.90 μg/108 cells), capacitated spermatozoa (128.2±3.29 μg/108 cells) and acrosome reacted spermatozoa (115.7±2.04 μg/108 cells) were found significantly (P<0.05) different. During the process of capacitation and acrosome reaction (AR) there was substantial loss of total lipids. The total Lipid content of freshly ejaculated, capacitated and acrosome reacted spermatozoa were fractionated into three major classes namely, Phospholipids, Neutral lipids and Glycolipids. Among the classes of lipids; there was no change in concentration of glycolipids. Phospholipids in fresh, capacitated and acrosome reacted spermatozoa were 84.27±1.96, 58.40±2.40 and 50.51±1.27 μg/108 cells respectively. In case of Neutral lipids, the concentration were 33.63±2.62, 22.55±1.86 and 11.61±0.87 μg/108 cells in fresh, capacitated and acrosome reacted spermatozoa respectively. The results generated by this study will be one step further to understand the lipid changes in buffalo spermatozoa during capacitation, acrosome reaction and fertilization process.

scholarly journals Comparison of lipid compositions of soft coral Sinularia brassica collected in different coastal regions of Vietnam

2019 ◽  
Vol 19 (2) ◽  
pp. 285-291
Author(s):  
Dang Thi Phuong Ly ◽  
Nguyen Thi Nga ◽  
Dang Thi Minh Tuyet ◽  
Pham Minh Quan ◽  
Andrey Imbs Borisovich
Keyword(s):  
Fatty Acids ◽  
Total Lipid ◽  
Lipid Content ◽  
Soft Coral ◽  
Total Lipid Content ◽  
Total Lipids ◽  
Soft Corals ◽  
Two Samples ◽  
Nha Trang Bay ◽  
Quang Ninh

Five soft coral samples belonging to the same specimen Sinularia brassica were collected in the coastal area of Ha Long Quang Ninh, Nhat Le beach, Quang Binh, Hon Vang - Thanh Hoa and Nha Trang areas (2014–2016), these samples were examined and analyzed by total lipid content, content and composition of lipid and fatty acids. Highest total lipid content was found in the sample SHM 09 collected in Nha Trang bay (1.11%), and the lowest total lipid content was in HL-SHM 07 collected in Ha Long, Quang Ninh (0.68%).The lipid composition of samples contained basic classes such as polar lipids, sterols, free fatty acids, triacylglycerols, monoankyldiacylglycerols, hydrocarbons and waxes. In studied soft corals, there are the appearance of all fatty acids from C12 to C24. There are differences in composition and content of lipids and fatty acids in samples collected in different locations: Samples collected at Nha Trang bay contain more polyunsaturated fatty acids than the other samples collected in Ha Long, Thanh Hoa, Quang Binh. Another two samples, collected in Hon Vang (Thanh Hoa) have similar composition and content of lipid classes, the composition and content of fatty acids in total lipids. The obtained results also show the significant impact of habitat and time of acquisition on the studied subject.

scholarly journals FATTY ACID PROFILES OF CYCLOPOID COPEPOD NAUPLII Apocyclops panamensis AND THE EFFECTS OF SALINITY CHANGE

2007 ◽  
Vol 2 (2) ◽  
pp. 75
Author(s):  
Gede Suwarthama Sumiarsa ◽  
Ronald P. Phelps
Keyword(s):  
Fatty Acid ◽  
Total Lipid ◽  
Lipid Content ◽  
Polar Lipid ◽  
Sea Water ◽  
Neutral Lipids ◽  
Total Lipid Content ◽  
Water Salinity ◽  
Cyclopoid Copepod ◽  
Fatty Acid Profiles

<p>Lipid and fatty acid profiles were described for copepod nauplii Apocy clops panamensis from fertilized brackish water ponds, and after being acclimated to fullsea water salinity. Mean total lipid content of copepod nauplii collected from ponds fertilized with inorganic fertilizer combined either with alfalfa meal, rice bran, wheat bran, and a combination of these fertilizers ranged from 5.66 ± 0.15 to 7.76% ± 0.27%. Non-polar (neutral) lipid fraction of pond copepod nauplii was a significantly higher percentage of the total lipid content (74.5 ± 1.8 - 93.5% ± 1.0%) compared to those of polar lipid (6.5 ± 1.0 - 21.3% ± 1.8%) (P= 0.000). DHA/EPA ratio in neutral lipids ranged from 1.8 ± 0.2 - 2.0 ± 0.1 with no significant differences in three fertilization regimes. DHA was 27.5% ± 0.56% of the neutral lipids and EPA 14.8% ± 0.8%. Acclimation of copepod nauplii for six hours from brackish to full-sea water salinity reduced their lipid content and individual dry weight significantly. Mean total lipid content was reduced 44.2%, non-polar lipid was reduced 46.9% and polar lipid was reduced 24.4%. Acclimation altered the DHA/EPA ratio, in the neutral fraction the ratio increased 26.3% but in the polar fraction it decreased 25%.</p>

Comparison of three microquantity techniques for measuring total lipids in fish

2008 ◽  
Vol 65 (10) ◽  
pp. 2233-2241 ◽  
Author(s):  
Yuehan Lu ◽  
Stuart A. Ludsin ◽  
David L. Fanslow ◽  
Steven A. Pothoven
Keyword(s):  
Total Lipid ◽  
Lipid Content ◽  
Yellow Perch ◽  
Lake Michigan ◽  
Total Lipid Content ◽  
Coregonus Clupeaformis ◽  
Total Lipids ◽  
Flame Ionization ◽  
High Lipid ◽  
Layer Chromatography

To measure lipids in juvenile and adult fishes, we refined three microquantity approaches (microgravimetric, microcolorimetric sulfophosphovanillan (SPV), and Iatroscan thin layer chromatography – flame ionization detection (TLC–FID)) that were originally developed to measure lipids in small aquatic invertebrates. We also evaluated their precision and comparability by quantifying the total lipid content of age-1+ walleye ( Sander vitreus ), yellow perch ( Perca flavescens ), and lake whitefish ( Coregonus clupeaformis ) collected in Lake Erie (US–Canada), Lake Michigan (USA), and Muskegon Lake (Michigan, USA). Our findings demonstrate that (i) microquantity approaches provide estimates of total lipids in juvenile and adult fishes similar to those of more traditional macroquantity approaches, (ii) the microcolorimetric SPV and microgravimetric approaches produce near identical estimates of total lipid content, and (iii) the Iatroscan TLC–FID approach underestimates total lipids relative to the other approaches for individuals with high lipid levels. Ultimately, our research makes available additional techniques for measuring total lipid content of fishes that are less expensive than traditional techniques, owing to a reduced need for large quantities of samples and solvents.

Lipid content of cured tobacco

1974 ◽  
Vol 52 (5) ◽  
pp. 1123-1126 ◽  
Author(s):  
Momtaz K. Wassef ◽  
James W. Hendrix
Keyword(s):  
Total Lipid ◽  
Lipid Content ◽  
Lipid Composition ◽  
Neutral Lipids ◽  
Total Lipid Content ◽  
Lipid Classes ◽  
Minor Effect ◽  
Burley Tobacco ◽  
A Minor ◽  
High Polarity

Samples from cured burley and flue-cured tobacco were examined for total lipid content and for lipid composition of fractionated lipid classes. Cured burley tobacco contained 6–8% lipids compared with 14–15% in cured flue-cured tobacco. Neutral lipids constituted 70–80% of burley lipids but only half the flue-cured lipids; the latter had 75% more neutral lipids than the former. Flue-cured tobacco contained considerably more (5–10 times) medium- to high-polarity lipids than burley. Stalk position had only a minor effect on the lipid content of both tobacco types.

scholarly journals Effect of season on the fatty acid profile of total lipids, phospholipids and triacylglycerols in Mastacembelus mastacembelus (Atatürk Dam Lake, Turkey)

2018 ◽  
Vol 69 (1) ◽  
pp. 242 ◽  
Author(s):  
S. Kaçar ◽  
M. Başhan ◽  
S. A. Oymak
Keyword(s):  
Fatty Acids ◽  
Total Lipid ◽  
Lipid Content ◽  
Palmitoleic Acid ◽  
Saturated Fatty Acids ◽  
Total Lipid Content ◽  
Docosapentaenoic Acid ◽  
Monounsaturated Fatty Acids ◽  
Total Lipids ◽  
Atatürk Dam Lake

The present study investigated the lipid content and FA profiles of total lipids, phospholipids (PL) and triacylglycerols (TAG) of Mastacembelus mastacembelus from the Atatürk Dam Lake (Turkey). The results showed that the total lipid content of the dorsal muscle varied seasonally from 0.50% to 3.59%. Wide ranges of saturated fatty acids (SFAs) (36.21 to 50.52%) and polyunsaturated fatty acids (PUFAs) (25.22 to 42.02%) were found in the PL fraction. However, higher monounsaturated fatty acids (MUFAs) (37.63 to 45.07%) and SFA (34.71 to 38.82%) were found in TAG (triacylglycerol). The ratios of ω-3 to ω-6 PUFA ranged from 0.65 to 1.32 and 1.07 to 3.48 in PL (phospholipid) and TAG fraction, respectively. The results also showed that the major components were palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1ω-9), palmitoleic acid (C16:1ω-7), docosahexaenoic acid (DHA, C22:6ω-3), arachidonic acid (AA, C20:4ω-6) and docosapentaenoic acid (C22:5ω-3) in total lipid, C16:0, C18:1ω-9, AA and DHA in PL, C16:0, C16:1ω-7, C18:1ω-9, linoleic acid (LA, C18:2ω-6), and DHA in TAG extracted from the muscle of M. mastacembelus in all seasons.

Quantification of total lipids based on the characteristic absorption of tetra-β-(2-octanyloxy)-substituted nickel phthalocyanine

2019 ◽  
Vol 11 (43) ◽  
pp. 5629-5637
Author(s):  
Ping Li ◽  
Fangdi Cong ◽  
Shulin Zhang ◽  
Wenli Zhou ◽  
Hualing Zhu ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Total Lipid ◽  
Lipid Content ◽  
Total Lipid Content ◽  
Total Lipids ◽  
Characteristic Absorption ◽  
Nickel Phthalocyanine

Molecular mechanism for the quantification of the total lipid content based on ONPc chromophores.

scholarly journals Identification of the molecular regulation of differences in lipid deposition in dedifferentiated preadipocytes from different chicken tissues

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Zheng Ma ◽  
Na Luo ◽  
Lu Liu ◽  
Huanxian Cui ◽  
Jing Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Lipid Content ◽  
Regulatory Network ◽  
Total Lipid Content ◽  
Fatty Acid Transport ◽  
Lipid Deposition ◽  
Ppar Signaling ◽  
The Difference

Abstract Background A body distribution with high intramuscular fat and low abdominal fat is the ideal goal for broiler breeding. Preadipocytes with different origins have differences in terms of metabolism and gene expression. The transcriptome analysis performed in this study of intramuscular preadipocytes (DIMFPs) and adipose tissue-derived preadipocytes (DAFPs) aimed to explore the characteristics of lipid deposition in different chicken preadipocytes by dedifferentiation in vitro. Results Compared with DAFPs, the total lipid content in DIMFPs was reduced (P < 0.05). Moreover, 72 DEGs related to lipid metabolism were screened, which were involved in adipocyte differentiation, fatty acid transport and fatty acid synthesis, lipid stabilization, and lipolysis. Among the 72 DEGs, 19 DEGs were enriched in the PPAR signaling pathway, indicating its main contribution to the regulation of the difference in lipid deposition between DAFPs and DIMFPs. Among these 19 genes, the representative APOA1, ADIPOQ, FABP3, FABP4, FABP7, HMGCS2, LPL and RXRG genes were downregulated, but the ACSL1, FABP5, PCK2, PDPK1, PPARG, SCD, SCD5, and SLC27A6 genes were upregulated (P < 0.05 or P < 0.01) in the DIMFPs. In addition, the well-known pathways affecting lipid metabolism (MAPK, TGF-beta and calcium) and the pathways related to cell communication were enriched, which may also contribute to the regulation of lipid deposition. Finally, the regulatory network for the difference in lipid deposition between chicken DAFPs and DIMFPs was proposed based on the above information. Conclusions Our data suggested a difference in lipid deposition between DIMFPs and DAFPs of chickens in vitro and proposed a molecular regulatory network for the difference in lipid deposition between chicken DAFPs and DIMFPs. The lipid content was significantly increased in DAFPs by the direct mediation of PPAR signaling pathways. These findings provide new insights into the regulation of tissue-specific fat deposition and the optimization of body fat distribution in broilers.

Aspergillus flavusgrowth and aflatoxin production as influenced by total lipid content during growth and development of cottonseed

2017 ◽  
Vol 31 (1) ◽  
pp. 91-99 ◽  
Author(s):  
Kanniah Rajasekaran ◽  
Greg Ford ◽  
Kandan Sethumadhavan ◽  
Carol Carter-Wientjes ◽  
John Bland ◽  
...  
Keyword(s):  
Total Lipid ◽  
Lipid Content ◽  
Growth And Development ◽  
Total Lipid Content ◽  
Aflatoxin Production

Comparison of biomass production and total lipid content of freshwater green microalgae cultivated under various culture conditions

2013 ◽  
Vol 37 (2) ◽  
pp. 99-106 ◽  
Author(s):  
Geun Ho Gim ◽  
Jung Kon Kim ◽  
Hyeon Seok Kim ◽  
Mathur Nadarajan Kathiravan ◽  
Hetong Yang ◽  
...  
Keyword(s):  
Biomass Production ◽  
Total Lipid ◽  
Lipid Content ◽  
Total Lipid Content ◽  
Culture Conditions ◽  
Green Microalgae
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