The influence of injectable sodium selenite on semen characteristics and testosterone concentration in Aardi goats

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
A. Hajalshaikh ◽  
M. J. Al-Hassan ◽  
H. E. Mohamed
Keyword(s):  
Body Weight ◽  
Sodium Selenite ◽  
Semen Quality ◽  
Sperm Count ◽  
Plasma Concentrations ◽  
Control Group ◽  
Progressive Motility ◽  
Group I ◽  
Semen Characteristics ◽  
And Control

The objective of this study was to test the effects of sodium selenite (SS) injection on semen characteristics and testosterone plasma concentrations in male Aardi goats. Fifteen animals were assigned into three groups; control (group I, with no supplemental SS); group II; injected intramuscular with 0.1 mg/kg SS body weight; group III injected 0.05 mg/kg SS body weight. Blood samples were collected once weekly (week 0 and four weeks after treatment) in the morning (at 8 am). Semen was collected by electro-ejaculator on a weekly basis post SS supplementation. Sperm count, motility %, progressive motility characteristic; average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), straightness (STR) and linearity (LIN) were analyzed. Significant differences were found between the two treated-groups and control after week four for motility and progressive motility. After five weeks, significant differences were found between groups, for motility and progressive motility. The live % showed significant differences after five weeks between treated and control groups. After five weeks, significant differences were found between the three groups, for motility and progressive motility. The live % showed significant differences after 5 weeks between different treatments. However, VAP, VSL, VCL, STR, and LIN showed non-significant differences, suggesting that bucks might already have a good motility characteristic. No significant effects of SS supplementation on testosterone plasma levels, and the reported values were 1.28; 1.35, and 1.36 ng/ml for control; group 1, and group 2; respectively. In conclusion, SS improved reproduction in goats <italic>via</italic> the enhancement of semen quality in Aardi goats.

TESTOSTERONE DECREASE AND OESTROGEN INCREASE IN MALE PATIENTS WITH OBESITY

1979 ◽  
Vol 91 (3) ◽  
pp. 553-563 ◽  
Author(s):  
H. K. Kley ◽  
H. G. Solbach ◽  
J. C. McKinnan ◽  
H. L. Krüskemper
Keyword(s):  
Body Weight ◽  
Sex Hormones ◽  
Plasma Concentrations ◽  
Clinical Signs ◽  
Free Testosterone ◽  
Control Group ◽  
Obese Patients ◽  
Group Iii ◽  
Group I ◽  
Male Patients

ABSTRACT The concentration of sex hormones and their binding in the plasma were measured in male patients (20–40 years old), who weighed 140–170 % (I), 170–200 % (II) and > 200 % (III) of their ideal weight. Correlated to body weight, there is a reduction in the total concentration of testosterone, which, in the very obese patients, amounts to 41 % (in group I: 85 % in group II: 68 % P < 0.001) of that found in an age-matched healthy control group of subjects of "normal" body weight (90–115 % of the ideal body weight; n: 20). Androstenedione values show only a trend downwards (from 0.94 to 0.72 ng/ml plasma), while the oestrogen values increase significantly; oestrone increases by a factor of 1.09 (I), 1.43 (II; P < 0.001) and 1.69 (III; P < 0.001) and oestradiol by 1.13, 1.43 P < 0.001) and 1.76 (P < 0.001), respectively. Despite the fall in testosterone there are no clinical signs of hypogonadism, as SHBG (from 5.1 ± 0.8 in the controls to 2.4 ± 0.6 ×10−8 Mol/l in the very obese patients of group III) and the protein-bound fraction of testosterone also decrease. As a result the concentration of free testosterone remains constant (120 pg/ml), except in the very obese (93 pg/ml). Because of the different affinity of the binding proteins for testosterone and oestradiol the ratio of free oestradiol: free testosterone shifts less strongly in favour of the feminizing hormone (11.1 × 10−3 in group III as compared to 4.1 × 10−3 in the controls), than is suggested by the total hormone concentrations. A disturbance in the gonadal function of the pituitary gland or the testes is not present, since the concentration of LH is normal and the testicular response to HCG in very obese patients adequate (increase of testosterone by a factor of 3.11 as compared to 2.23 in the controls). The cause of the decrease in testosterone and SHBG is unknown, while the increase of plasma oestrogens is likely to be due to the increased conversion of androgens to oestrogens in the adipose tissue, which clearly plays an important role for plasma concentrations of sex hormones in obese patients.

scholarly journals Effect of Rambutan Fruit Peel Extract on Total Sperm Counts of Wistar Rats With Obesity

Biota ◽  
2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Kerin Victoria Sipahutar ◽  
Yudhi Nugraha ◽  
Cut Fauziah
Keyword(s):  
Wistar Rats ◽  
Semen Quality ◽  
Sperm Count ◽  
Control Group ◽  
Antioxidant Compounds ◽  
P Value ◽  
High Fat ◽  
Fruit Peel ◽  
Group I ◽  
Peel Extract

Obesity caused by a high-fat diet leads to an altered reproductive hormonal profile, including impaired semen quality. Antioxidants can overcome these conditions. One of the well-known sources of antioxidants is in the rambutan fruit peel extract. This research aimed to figure the effect of rambutan fruit peel extract towards total sperm count in Wistar rats induced with high-fat feed. The study design used post-test only control group,  subjects were 30 male Wistar rats divided into five groups: Group I (Positive Control) was given high-fat feed, Group II (Treatment Control Group) was given 15mg/kg BW rambutan fruit peel extract, whereas group III, IV, V (Treatment Group) were given an extract of rambutan skin with a dosage of 15, 30, and 60 mg/kg BW, respectively. Treatment was administered for 81 days. This study showed that rambutan fruit peel extract with doses of 15, 30, and 60 mg/kg BW has significantly increased total sperm count in Wistar rats induced with high-fat feed. The outcome using Kruskal Wallis shows a result of p-value 0,010 (CI 95%). Antioxidant compounds found in rambutan fruit peel extract significantly increase total sperm count in Wistar rats induced with high-fat feed, with the highest efficacy on the dose of 15mg/kg BW.   Keywords: Fruit Peel Rambutan Extract; ; ; 

33 Effect of exogenous melatonin administration on testicular biometry, libido parameters, seminal parameters, hormone levels, and freezability of Sirohi buck semen during the non-breeding season in Southern Rajasthan

2021 ◽  
Vol 33 (2) ◽  
pp. 124
Author(s):  
C. S. Sarswat ◽  
S. Sharma ◽  
K. Nehra ◽  
P. C. Sharma ◽  
M. C. Parashar ◽  
...  
Keyword(s):  
Breeding Season ◽  
Corn Oil ◽  
Semen Quality ◽  
Sperm Count ◽  
Control Group ◽  
Exogenous Melatonin ◽  
Hormone Levels ◽  
Progressive Motility ◽  
Seminal Parameters ◽  
Breeding Seasons

The aim of this work was to determine the effect of administration of exogenous melatonin on the testicular biometry, libido parameters, hormone levels, and freezability of semen during the non-breeding seasons in adult Sirohi bucks. Experiments of 4 and 8 weeks were carried out during non-breeding seasons at different times. Twelve bucks, 2 to 3 years of age, were split into melatonin-treated and control groups, with 6 bucks in each group. Throughout the 2 experimental periods, all bucks were permanently kept outdoors under conditions of natural daylength and at ambient temperature. Bucks in the treated group were subjected to single subcutaneous injection of a powder form of melatonin mixed with corn oil at the dose rate 18mg per 50kg on the first day of experiment; bucks in the control group received sterile corn oil injected subcutaneously in control group. Ejaculates were taken from the bucks by artificial vagina (AV) once per week and their reaction time (the elapsed time between exposure to stimuli and first ejaculation) was recorded. Testicular biometry, serum testosterone, and serum melatonin were determined weekly before semen collection. Ejaculates were cryopreserved and thawed weekly and the same quantitative and qualitative variables were measured as for fresh semen samples. Melatonin significantly (P&lt;0.05) improved testicular biometry, libido parameters, and hormone levels compared with the control group during weeks 3 and 4 of the first experiment and from week 5 onward of the second experiment (P&lt;0.05). Semen volume (mL) improved to 0.70±0.039 vs. 0.48±0.009 and 0.94±0.036 vs. 0.56±0.032 in first and second experiments. Greater (P&lt;0.05) progressive motility (%) was noted in the melatonin group (84.59±1.472 and 86.88±0.797) during experiments 1 and 2. Semen quality of each straw from each ejaculate was evaluated after proper thawing within 24h. Mean post-thaw live sperm percentage for experiments 1 and 2 was 69.25±0.382 and 66.90±0.779, progressive motility was 50.42±0.948 and 58.75±1.058, and curled tail sperm count were 51.00±0.715 and 61.36±0.773, respectively. In conclusion, the results of present experiments are novel regarding the use of single subcutaneous melatonin injection during the non-breeding season to improve variables associated with the fertility potential of the Sirohi buck and frozen-thawed seminal parameters.

scholarly journals Effects of Chronic Use of Methylphenidate on Spermatogenesis and Sexual Hormones in Adult Male Rats

2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Alireza Akhavan Rezayat ◽  
Amir Abbas Asadpour ◽  
Samaneh Boroumand Noughabi ◽  
Hassan Ahmadnia ◽  
Hamid Mohseni ◽  
...  
Keyword(s):  
Body Weight ◽  
Sperm Count ◽  
Total Body Weight ◽  
Male Rats ◽  
Control Group ◽  
Testis Weight ◽  
Control Groups ◽  
Major Health Problem ◽  
Total Body ◽  
And Control

Background: The growing prevalence of Attention Deficit Hyperactivity Disorder (ADHD) and the non-medical use of Methylphenidate (MPH) among the youth have lead male infertility to be a major health problem. Objectives: The present study was conducted to investigate the impacts of MPH administration on different aspects of productivity, including total body weight, testis weight, spermatogenesis, sperm motility, histopathology changes, and sex hormone serum levels in male rats. Methods: This study was performed with 54 eight-week-old male rats divided into one control and two experimental groups. The experimental groups were gavaged with 2 and 10 mg/kg methylphenidate daily while the control group was gavaged with normal saline (at the same dosage). After 60 days, rats were subjected to blood sampling and bilateral orchidoepididymectomy under anesthesia. Spermogram, histological, and hormonal evaluations were performed on the samples. Testes weight and total body weight were also recorded. Results: The results revealed significant differences between the MPH and experimental groups in terms of hormonal, spermographic, and histopathologic features, as well as weight. Luteinizing hormone and testosterone levels, sperm count and motility, Leydig cell hyperplasia, spermatogenesis, congestion and necrosis levels, total body weight, and testis weight were significantly different between the experimental and control groups. However, no difference was observed between the experimental and control groups concerning follicle-stimulating hormone, maturation arrest, and edema levels. Conclusions: Based on the findings, MPH exposure exerts a significant effect on the testis and total body weight, as well as hormonal, spermatographic, and histopathologic characteristics. Accordingly, the present study provided an insight into the negative impression of MPH on sexual parameters.

Effect of optimization of the levels of dissolved oxygen in semen extender on physico-morphological attributes and functional membrane integrity of crossbred bull spermatozoa

2018 ◽  
Author(s):  
Abhishek Kumar ◽  
J. K. Prasad ◽  
A. R. Mustapha ◽  
R. Mustapha ◽  
G. K. Das ◽  
...  
Keyword(s):  
Dissolved Oxygen ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Control Group ◽  
Morphological Attributes ◽  
Progressive Motility ◽  
Group I ◽  
Functional Membrane ◽  
Group Ii ◽  
Semen Extender

The objective of present study was to investigate the effects of two different levels of dissolved oxygen (DO) i.e. 4 and 8 ppm in the extenders on physico-morphological attributes and functional membrane integrity of crossbred bull spermatozoa following cryopreservation. Only those ejaculates that had a mass activity of ³ 3+ and individual progressive motility of ³ 70% were utilized. Each of the ejaculates were divided and extended to have 80×106 spermatozoa/ mL of extender in three groups i.e. Group I (control), Group II (4 ppm) and Group III (8 ppm). Progressive motility (%) was higher (p Lass Than 0.05) in Group II and III as compared to Group I at both pre-freeze and post-thaw stage. Per cent live sperm and HOST reactive sperm were higher (p Lass Than 0.05) in Group II and in Group II and III as compared to Group I at pre-freeze and post-thaw stage, respectively. Sperm abnormality (%) was lower (p Lass Than 0.05) in Group II at post-thaw stage. Per cent acrosome intact sperm did not differ (p>0.05) among the groups at pre-freeze stage, but, were higher (p Lass Than 0.05) in Group II and III as compared to Group I at post-thaw stage. In conclusion, cryopreservation of semen having DO level of 4 ppm in semen extender yielded better results followed by 8 ppm in terms of semen quality parameters and functional membrane integrity of crossbred bull spermatozoa.

Partial Deoxygenation of Semen Extender Minimizes Post-thaw Damages and Improves Freezability of Crossbred Bull Spermatozoa

2021 ◽  
Author(s):  
Lhendup Bhutia ◽  
Abhishek Kumar ◽  
Rahul Katiyar ◽  
Vinod Gupta ◽  
M. Ramamoorthy ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Control Group ◽  
Progressive Motility ◽  
Group I ◽  
Group Ii ◽  
Semen Extender ◽  
Acrosomal Integrity

Background: Oxidative stress occurs when oxygen or oxygen derived oxidants exceed antioxidants and become responsible for poor post-thaw semen quality during the cryopreservation process. Therefore, the present study was designed to investigate the effects of dissolved oxygen (DO) levels (4, 6 and 8 ppm) in semen extender on freezability of crossbred bull spermatozoa. As the gap between 4 and 8 ppm existed in earlier studies, DO of 6 ppm in semen extender was further standardized and the effects were compared with other respective groups to contemplate any improvement in post-thaw semen quality.Methods: For the experiment, Tris-egg Yolk-Glycerol (TYG) extender was partially deoxygenated by nitrogen gassing @ 2-3 bubbles/sec at 34°C for 0, 16, 12 and 9 minutes to obtain DO levels of 11.7 ppm (Group-I/control), 4 ppm (Group-II), 6 ppm (Group-III) and 8 ppm (Group IV), respectively and collected semen samples were diluted with these extender groups to have 80×106 spermatozoa/ ml of the extender. Semen samples were evaluated for individual progressive motility (IPM), lipid peroxidation (LPO), total antioxidant capacity (TAC), plasma membrane integrity, acrosomal integrity and apoptotic changes at different stages of cryopreservation.Result: At the post-thaw stage, progressive motility was greater (p less than 0.05) in Group II compared to Group I and the least reduction from the post-dilution to the post-thaw stage was observed in Group II. In comparison to Group I, Groups II, III and IV showed lesser (p less than 0.05) MDA production with Group II having greater (p less than 0.05) TAC concentration than other groups at the post-thaw stage. A declining trend was observed in membrane integrity as DO levels increased from 4 ppm to 11.7 ppm. Acrosomal integrity did not differ among treatment groups, but, found to be higher (p less than 0.05) than the control group. Per cent viable spermatozoa was greater (p less than 0.05) in Group II than Group I and vice versa for necrotic spermatozoa as assessed by Annexin VFITC/PI staining. In conclusion, reducing the DO level to 4 ppm before cryopreservation improved the freezability by reducing oxidative stress and apoptotic changes while, above 4 ppm tended to lower it. An appreciable improvement in freezability can be seen at 6 ppm of DO, but, not up to that extent as observed at 4 ppm.

Protective effect of sodium selenite and zinc sulfate on intensive swimming-induced testicular gamatogenic and steroidogenic disorders in mature male rats

2008 ◽  
Vol 33 (5) ◽  
pp. 903-914 ◽  
Author(s):  
Kuladip Jana ◽  
Pravat K. Samanta ◽  
Indranil Manna ◽  
Prasanta Ghosh ◽  
Narendra Singh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Zinc Sulfate ◽  
Sodium Selenite ◽  
Sperm Count ◽  
Plasma Concentrations ◽  
Plasma Corticosterone ◽  
Seminiferous Epithelium ◽  
Male Rats ◽  
Control Group ◽  
Control Groups

To investigate the ameliorative potential of sodium selenite and zinc sulfate on intensive-swimming-induced testicular disorders, 48 Wistar male rats (age, 4 months; mass, 146.2 ± 3.6 g) were randomly divided into 4 groups: the unexercised-control group (n = 12); the exercised group (n = 12); the control supplemented group (n = 12); and the exercised supplemented group (n = 12). For 10 weeks, the exercised rats underwent a protocol that consisted of 4 h·d–1swimming, for 6 d·week–1; the control rats did not exercise. For 10 weeks, both the supplemented groups received an oral daily dose of a combination of sodium selenite and zinc sulfate (6  and 3 mg·kg body mass–1, respectively). After 10 weeks, a significant reduction (p < 0.05) was seen in rats in the exercised group, compared with rats in both control groups, in paired testicular masses; in epididymal sperm count; in testicular Δ5, 3β-hydroxysteroid dehydrogenase (HSD) and 17β-HSD; in plasma levels of testosterone, luteinizing hormone, follicle-stimulating hormone, and prolactin; in the numbers of preleptotine spermatocytes, midpachytene spermatocytes, and stage 7 spermatids of the stage VII seminiferous epithelium cycle; and in fertility performance. As well, a significant increase (p < 0.05) was seen in the exercised group, compared with both control groups, in plasma corticosterone levels and in testicular content of malondialdehyde and catalase activity. At the same time, there was a significant reduction (p < 0.05) in the exercised group, compared with both control groups, in plasma concentrations of zinc and selenium; in the testicular content of glutathione (GSH), the glutathione and glutathione disulphide (GSSG) ratio, ascorbic acid, and α-tocopherol; and in testicular activities of superoxide dismutase, glutathione-peroxidase, and glutathione-S-transferase in the testes. No significant changes were seen in the number of spermatogonia-A from the stage VII seminiferous epithelium cycle or the testicular content of GSSG among the groups. Sodium selenite and zinc sulfate supplementation significantly protected against exercise-induced testicular gamatogenic and spermatogenic disorders, prevented testicular oxidative stress, and increased antioxidant status. It can be concluded that intensive-swimming-induced oxidative stress causes dysfunctions in the male reproductive system, which can be protected by the coadministration of sodium selenite and zinc sulfate.

Efficacy of Ovsynch and Ovsynch Plus Protocol for Improvement of Fertility in Postpartum Sahiwal Cows

2019 ◽  
Vol 14 (04) ◽  
Author(s):  
J. P. Lakher ◽  
M. K. Awasthi ◽  
J. R. Khan ◽  
M. R. Poyam
Keyword(s):  
Fixed Time ◽  
Conception Rate ◽  
Control Group ◽  
Group Iii ◽  
Treatment Groups ◽  
Group I ◽  
Early Postpartum ◽  
The Status ◽  
Sahiwal Cows ◽  
And Control

The present study was conducted to investigate the efficacy of Ovsynch and Ovsynch plus protocol in postpartum (day 60) Sahiwal cows (n=18). Animals were randomly divided into three equal groups, viz., Ovsynch group Ovsynch plus group and Control group. Animals of group I (n = 6) were treated with traditional Ovsynch protocol. The animals (n = 6) of group II were treated with Ovsynch plus protocol which consisted of an initial intramuscular injection of eCG (Folligon) @ 250 IU on day 60 postpartum followed 3 days later by GPG (Ovsynch) protocol. In group-III Control, no treatment was given to animals (n = 6). Treated animals were inseminated at a fixed time between 14 and 20 hrs after second GnRH injection, irrespective of estrus detection. Blood samples were collected from each animal on days 50 and 60 postpartum to determine the status of cyclicity in animals based on serum concentrations of progesterone (P4). A third blood sample was collected on the day of prostaglandin treatment to determine the response of first GnRH injection. Four animals each were cyclic, and two were acyclic in both treatment groups. Four animals each responded to first GnRH treatment in both treatment groups. Similarly, two animals each got conceived giving conception rate of 50% (2/4) in each treatment. In the control group, one out of 6 animals got conceived yielding 16.66 % conception rate (1/6) during the study period. It may be thus concluded that Ovsynch and Ovsynch plus protocol may be used during the early postpartum period to improve the reproductive efficiency in postpartum Sahiwal cows.

Impact of an Infant Formula Containing a Novel Fat Blend (Cow’s Milk Fat, Fish and Vegetable Oil) and Prebiotics on Stool Fatty Acid Soaps and Erythrocyte Fatty Acid Profiles in Full-Term Healthy Newborns

2021 ◽  
pp. 1-8
Author(s):  
Maroula Lambidou ◽  
Birgit Alteheld ◽  
Rolf Fimmers ◽  
Frank Jochum ◽  
Antonia Nomayo ◽  
...  
Keyword(s):  
Body Weight ◽  
Fatty Acid ◽  
Infant Formula ◽  
Milk Fat ◽  
Control Group ◽  
Human Breast Milk ◽  
Infant Formulas ◽  
Fa Composition ◽  
And Control ◽  
Breast Fed

<b><i>Introduction:</i></b> Recently, new commercial infant formulas have been composed considering novel fat blends and oligosaccharides to better resemble the fatty acid (FA) composition and stereospecific distribution (e.g., increased amount of ß-palmitate) as well as probiotics content of human breast milk. We hypothesized that these newly composed infant formulas may decrease fecal FA soap excretion and may positively affect erythrocyte FA profiles compared with regular formulas. <b><i>Methods:</i></b> Healthy infants were randomly assigned to receive a high-sn-2-palmitate formula (&#x3e;25% of the PA is esterified to the sn-2 position of the glycerol backbone, verum: <i>n</i> = 30) or a “standard” formula containing &#x3c;10% of PA in sn-2 position and no oligosaccharides (control: <i>n</i> = 27); a non-randomized group of breast-fed infants served as control. Anthropometric data of the infants (body weight, recumbent length, and head circumference) were recorded at inclusion (visit 1) and 6 and 12 weeks after onset of intervention (visits 2 and 3). Blood samples for erythrocyte FA analysis (gas chromatography) were taken at visits 1 and 2; stool samples were collected at visit 2. <b><i>Results:</i></b> Quantitative formula intake (mL/kg body weight × day) at visit 2 (verum: 155 ± 30, control: 164 ± 30) and visit 3 (verum: 134 ± 26, control: 134 ± 21) was comparable. Six weeks after onset of intervention, stool total FA soaps, palmitate soaps, and total FAs were similar in both formula-fed groups but significantly higher than in breast-fed infants. During the 6-week intervention, erythrocyte palmitate decreased significantly from baseline in all 3 groups with no group differences (verum: 29.20 ± 1.17 to 27.12 ± 0.66, control: 29.88 ± 2.00 to 27.01 ± 0.94, breast-fed: 30.20 ± 0.86 to 26.84 ± 0.98). For selected FAs, significant changes over time in verum and control group were obvious but without formula effects. Some variations in the FA profile of breast-fed infants compared to both verum and control groups were observed. <b><i>Conclusions:</i></b> In contrast to our hypothesis, feeding a newly composed infant formula based on a fat blend with 25% of PA in the sn-2 position of triacylglycerols and supplemented with a prebiotic could not decrease insoluble FA soap excretion compared with a standard product; in this respect, breastfeeding is obviously the best choice. Surprisingly, erythrocyte FA profiles were comparable in formula-fed and breast-fed infants; obvious alterations in FA composition of the respective fat sources and structure did not affect FA incorporation into membranes. Caution should be, however, exercised in drawing robust conclusions in the absence of larger, adequately powered intervention studies.

Potential anti-toxic effect of d-ribose-l-cysteine supplement on the reproductive functions of male rats administered cyclophosphamide

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Gabriel O. Oludare ◽  
Gbenga O. Afolayan ◽  
Ganbotei G. Semidara
Keyword(s):  
Body Weight ◽  
Single Dose ◽  
Sperm Motility ◽  
Sperm Count ◽  
Male Rats ◽  
Oxidative Enzymes ◽  
Protective Effects ◽  
Testosterone Levels ◽  
Group I ◽  
Antioxidant Defence System

Abstract Objectives This study aimed to access the protective effects of d-ribose-l-cysteine (DRLC) on cyclophosphamide (CPA) induced gonadal toxicity in male rats. Methods Forty-eight male Sprague-Dawley rats were divided into six groups of eight rats each. Group I the control, received distilled water (10 ml/kg), Group II received a single dose of CPA 100 mg/kg body weight intraperitoneally (i.p), Groups III and IV received a single dose of CPA at 100 mg/kg (i.p) and then were treated with DRLC at 200 mg/kg bodyweight (b.w) and 400 mg/kg b.w for 10 days, respectively. Rats in Groups V and VI received DRLC at 200 and 400 mg/kg b.w for 10 days, respectively. DRLC was administered orally. Results Results showed that CPA increased percentage of abnormal sperm cells and reduced body weight, sperm count, sperm motility, follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone levels (p<0.05). CPA also induced oxidative stress as indicated by the increased malondialdehyde (MDA) content and reduced activities of the oxidative enzymes measured (p<0.05). Liver enzymes were elevated while the blood cells production was decreased in the rats administered CPA. DRLC supplementation enhanced the antioxidant defence system as indicated in the reduced MDA levels and increased activities of the antioxidant enzymes when compared with CPA (p<0.05). Bodyweight, sperm count, sperm motility, FSH, and testosterone levels were increased in the CPA + DRLC II group compared with CPA (p<0.05). Conclusions The results of this present study showed that DRLC has a potential protective effect on CPA-induced gonadotoxicity.

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