33 Effect of exogenous melatonin administration on testicular biometry, libido parameters, seminal parameters, hormone levels, and freezability of Sirohi buck semen during the non-breeding season in Southern Rajasthan

2021 ◽  
Vol 33 (2) ◽  
pp. 124
Author(s):  
C. S. Sarswat ◽  
S. Sharma ◽  
K. Nehra ◽  
P. C. Sharma ◽  
M. C. Parashar ◽  
...  
Keyword(s):  
Breeding Season ◽  
Corn Oil ◽  
Semen Quality ◽  
Sperm Count ◽  
Control Group ◽  
Exogenous Melatonin ◽  
Hormone Levels ◽  
Progressive Motility ◽  
Seminal Parameters ◽  
Breeding Seasons

The aim of this work was to determine the effect of administration of exogenous melatonin on the testicular biometry, libido parameters, hormone levels, and freezability of semen during the non-breeding seasons in adult Sirohi bucks. Experiments of 4 and 8 weeks were carried out during non-breeding seasons at different times. Twelve bucks, 2 to 3 years of age, were split into melatonin-treated and control groups, with 6 bucks in each group. Throughout the 2 experimental periods, all bucks were permanently kept outdoors under conditions of natural daylength and at ambient temperature. Bucks in the treated group were subjected to single subcutaneous injection of a powder form of melatonin mixed with corn oil at the dose rate 18mg per 50kg on the first day of experiment; bucks in the control group received sterile corn oil injected subcutaneously in control group. Ejaculates were taken from the bucks by artificial vagina (AV) once per week and their reaction time (the elapsed time between exposure to stimuli and first ejaculation) was recorded. Testicular biometry, serum testosterone, and serum melatonin were determined weekly before semen collection. Ejaculates were cryopreserved and thawed weekly and the same quantitative and qualitative variables were measured as for fresh semen samples. Melatonin significantly (P<0.05) improved testicular biometry, libido parameters, and hormone levels compared with the control group during weeks 3 and 4 of the first experiment and from week 5 onward of the second experiment (P<0.05). Semen volume (mL) improved to 0.70±0.039 vs. 0.48±0.009 and 0.94±0.036 vs. 0.56±0.032 in first and second experiments. Greater (P<0.05) progressive motility (%) was noted in the melatonin group (84.59±1.472 and 86.88±0.797) during experiments 1 and 2. Semen quality of each straw from each ejaculate was evaluated after proper thawing within 24h. Mean post-thaw live sperm percentage for experiments 1 and 2 was 69.25±0.382 and 66.90±0.779, progressive motility was 50.42±0.948 and 58.75±1.058, and curled tail sperm count were 51.00±0.715 and 61.36±0.773, respectively. In conclusion, the results of present experiments are novel regarding the use of single subcutaneous melatonin injection during the non-breeding season to improve variables associated with the fertility potential of the Sirohi buck and frozen-thawed seminal parameters.

106 Exogenous melatonin administration improves behavioural signs, serum melatonin, testosterone, and semen quality in male dromedary (Camelus dromedarius) camels

2021 ◽  
Vol 33 (2) ◽  
pp. 160
Author(s):  
S. Dholpuria ◽  
G. N. Purohit ◽  
S. Vyas
Keyword(s):  
Reaction Time ◽  
Breeding Season ◽  
Corn Oil ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Serum Testosterone ◽  
Entire Study ◽  
Seminal Parameters ◽  
Breeding Seasons ◽  
And Control

The present study aimed to evaluate the effects of exogenous administration of melatonin to male camels on reproductive behaviour, seminal parameters, and serum concentrations of melatonin and testosterone during the non-breeding and subsequent breeding seasons. In September 2018, male camels (n=12) were randomly allocated to subcutaneous injections of either melatonin (MLT, 0.643 mg/kg; n=6) dissolved in corn oil, or plain corn oil; (control n=6). The camels were observed for behavioural signs of rut. A second dose of either MLT (1 mg/kg) or corn oil (control) was administered on 7 October 2018 subcutaneously, as camels did not show any change in behaviour until 6 October 2018, and the camels were again observed for behavioural signs of rut. Blood was collected from all camels on 1 September 2018, and then at weekly intervals until 7 January 2019. The serum was separated and stored until further assay of serum melatonin and testosterone using ELISA kits. Semen was collected from camels showing signs of rut period, and the reaction time, copulation time, and seminal parameters were evaluated. Data obtained was analysed by using the computer program SPSS (version 25; IBM Corp.), and means and standard errors were compared. Male reproductive behaviours (protrusion of soft palate, poll gland secretions, frequent urination, increased frothy salivation) were more pronounced in MLT-treated camels (83.33%) than in control camels (50%). Reaction time and copulation times were lower (112.81±30.06s and 229.8±37.91s) in MLT-treated camels than in controls (359.00±41.35s and 301.89±93.90s). Higher semen volume was observed in MLT-treated (5.34±2.06mL) than in control (3.11±1.75mL) camels (P<0.01). Semen collected from MLT-treated camels had more gel formation (81.25%), significantly higher sperm motility (67.50±2.32%), and increased sperm concentration (326.56±38.67 million mL−1) compared with samples obtained from the control males (P<0.01). Serum melatonin concentrations increased in both treated and control camels during the breeding season compared with concentrations before the breeding season. In MLT-treated camels, serum melatonin concentrations increased significantly and continued to be significantly higher than that in control camels for the entire study period (P<0.01). Serum testosterone concentrations were higher in both MLT-treated and control camels during the breeding season compared with those during the non-breeding season (P<0.01). We concluded that exogenous subcutaneous MLT administration improves sexual behaviour, seminal parameters, and serum testosterone concentrations during both the non-breeding and breeding seasons.

The influence of injectable sodium selenite on semen characteristics and testosterone concentration in Aardi goats

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
A. Hajalshaikh ◽  
M. J. Al-Hassan ◽  
H. E. Mohamed
Keyword(s):  
Body Weight ◽  
Sodium Selenite ◽  
Semen Quality ◽  
Sperm Count ◽  
Plasma Concentrations ◽  
Control Group ◽  
Progressive Motility ◽  
Group I ◽  
Semen Characteristics ◽  
And Control

The objective of this study was to test the effects of sodium selenite (SS) injection on semen characteristics and testosterone plasma concentrations in male Aardi goats. Fifteen animals were assigned into three groups; control (group I, with no supplemental SS); group II; injected intramuscular with 0.1 mg/kg SS body weight; group III injected 0.05 mg/kg SS body weight. Blood samples were collected once weekly (week 0 and four weeks after treatment) in the morning (at 8 am). Semen was collected by electro-ejaculator on a weekly basis post SS supplementation. Sperm count, motility %, progressive motility characteristic; average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), straightness (STR) and linearity (LIN) were analyzed. Significant differences were found between the two treated-groups and control after week four for motility and progressive motility. After five weeks, significant differences were found between groups, for motility and progressive motility. The live % showed significant differences after five weeks between treated and control groups. After five weeks, significant differences were found between the three groups, for motility and progressive motility. The live % showed significant differences after 5 weeks between different treatments. However, VAP, VSL, VCL, STR, and LIN showed non-significant differences, suggesting that bucks might already have a good motility characteristic. No significant effects of SS supplementation on testosterone plasma levels, and the reported values were 1.28; 1.35, and 1.36 ng/ml for control; group 1, and group 2; respectively. In conclusion, SS improved reproduction in goats <italic>via</italic> the enhancement of semen quality in Aardi goats.

scholarly journals Semen quality pattern and age threshold: a retrospective cross-sectional study of 71,623 infertile men in China, between 2011 and 2017

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
W. N. Li ◽  
M. M. Jia ◽  
Y. Q. Peng ◽  
R. Ding ◽  
L. Q. Fan ◽  
...  
Keyword(s):  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Cross Sectional Study ◽  
Semen Parameters ◽  
Sectional Study ◽  
Joinpoint Regression ◽  
Cross Sectional ◽  
Progressive Motility ◽  
Infertile Men

Abstract Background The aim of this study was to provide information on the semen quality pattern of infertile men and age thresholds for semen parameters in China. Methods This was a retrospective cross-sectional study investigating 71,623 infertile men from the Reproductive and Genetic Hospital of CITIC Xiangya in Hunan, China, from 2011 to 2017. The Kruskal-Wallis test, Mann-Kendall test, linear regression model and joinpoint regression were used. Results Although erratic changes were observed in the median semen parameters (sperm concentration 40.1–52.1 × 106/ml, total sperm count 117.8–153.1 × 106, sperm progressive motility 33.4–38.1%) during the 7 years of observation, no significant decrease in semen quality was found, and 47.88% of infertile men showed normal semen parameters according to the World Health Organization (WHO) criteria. According to the joinpoint regression analysis, sperm progressive motility appeared to decrease earlier than the sperm concentration and total sperm count (at 28, 58, and 42 years of age, respectively). Conclusions There is no evidence of a deterioration in semen quality among infertile men in Hunan, China. Semen parameters decreased with increasing age, with turning points noted at different ages. Semen parameters are not absolute evidence for the assessment of male fertility potential. Therefore, we believe that, among semen parameters, the sperm concentration is the best predictor of fertility for ART, followed by motility. Decreased sperm motility may affect natural pregnancy, but it is not necessary for successful IVF.

17 EFFECT OF LOCAL TREATMENT OF SEMINAL VESICULITIS ON THE QUALITY OF EQUINE FRESH SEMEN

2015 ◽  
Vol 27 (1) ◽  
pp. 101
Keyword(s):  
Semen Quality ◽  
Local Treatment ◽  
Membrane Integrity ◽  
Ringer Lactate ◽  
Plasma Membrane Integrity ◽  
Progressive Motility ◽  
Seminal Parameters ◽  
Fresh Semen ◽  
Lactate Solution

Stallions affected by seminal vesiculitis present history of infertility or subfertility, ejaculatory disturbance, spread of sexually transmitted pathogens, and changes in semen characteristics, leading to reduced semen quality and longevity. The aim of this study was to evaluate the semen quality of stallions with seminal vesiculitis before and after local treatment. Five stallions with a mean age of 12.4 years diagnosed with seminal vesiculitis were used. The identification of the microorganism involved in the pathogenesis of seminal vesiculitis of each animal was performed by bacterial culture of the seminal vesicles flush with Ringer Lactate solution, performed in duplicate at 1-week intervals. After identification of bacteria was performed, there was susceptibility testing to antibiotic (antibiogram) and the appropriate antibiotic was chosen. The local treatment was performed by endoscopy for 10 consecutive days, and this consisted of flushing with Ringer Lactate solution, followed by infusion of the antibiotic selected. The semen analyses were performed before starting the local treatment for seminal vesiculitis (M0), after a week (M1), and after a month (M2) of therapy. Sperm kinetics were performed by computerized method – CASA for the following parameters: percentage of sperm with total motility, progressive motility, and rapid sperm. Analysis of plasma membrane integrity was performed by epi-fluorescence microscopy, using the combination of fluorescent probes carboxyfluorescein diacetate and propidium iodide. Percentage of leukocytes was assessed through evaluation in light optical microscopy of semen smears stained with DiffQuick. The content of nitric oxide (NO) was determined by colourimetric Griess reaction by a spectrophotometer through the concentrations of nitrate (NO3–) and nitrite (NO2–). To perform the count of colony forming units per millilitre (CFU mL–1), an aliquot of 0.1 mL of semen was diluted in 9.9 mL of saline. A 0.1-mL aliquot of this sample was plated on Mueller-Hinton agar. The seeded plates were incubated, and the bacterial colonies were counted after 24 h. According to the performed dilution, total colonies identified corresponds to ×10 000 CFU mL–1. The data were analysed by two-way ANOVA followed by Tukey's test (P < 0.05). The values (mean ± standard error) of seminal parameters on M0, M1, and M2 were the following, respectively: sperm kinetics (total motility: 46.5 ± 5.13a; 75.1 ± 3.42b; 42.8 ± 5.28a; progressive motility: 19.3 ± 3.86a; 33.4 ± 2.39b; 16.5 ± 2.40a; rapid sperm: 22.2 ± 1.82a; 52.2 ± 5.65b; 22.1 ± 2.62a); plasma membrane integrity (47.5 ± 4.65a; 62.9 ± 5.41b; 39.1 ± 4.32a); percentage of leukocytes (35.2 ± 2.36a; 15.1 ± 2.55b; 36.1 ± 4.04a); CFU (119 980 × 103 ± 19 528.0 × 103a; 5375 × 103 ± 2453.7 × 103b; 65 850 × 103 ± 19 701.0 × 103ab) on fresh semen; and NO content (0.645 ± 0.172a, 0.117 ± 0.023b, 0.364 ± 0.110ab) on seminal plasma. The results demonstrate that local treatment after a week leads to an improvement in sperm quality; however, this was not maintained after 1 month of therapy, since the seminal parameters at this time are similar to pretreatment, which can be justified by recurrent disease.

scholarly journals Effect of Selenium and Vitamin E Supplementation on Semen Quality in Dogs with Lowered Fertility

2015 ◽  
Vol 59 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Anna Domosławska ◽  
Sławomir Zduńczyk ◽  
Wojciech Niżański ◽  
Andrzej Jurczak ◽  
Tomasz Janowski
Keyword(s):  
Vitamin E ◽  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Control Group ◽  
Organic Selenium ◽  
Healthy Dogs ◽  
Manual Manipulation ◽  
Motility Parameters

Abstract Thirty clinically healthy dogs with poor semen quality were used in the study. Fifteen dogs were supplemented daily with selenium (0.6 mg/kg organic selenium from yeast) and vitamin E (5 mg/kg) per os for 60 d. The control group (15 dogs) was not supplemented. Semen was collected from all dogs by manual manipulation on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated with a Hamilton Thorne sperm analyser, version IVOS 12.3. For the assessment of sperm morphology, Diff-Quik stain was used. The percentage of live and dead spermatozoa was estimated on dried smears stained with eosin-nigrosin. The concentration of spermatozoa, most motility parameters determined (PMOT, VSL, VCL, ALH, BCF, RAPID, MEDIUM, SLOW, and STATIC), and the percentage of spermatozoa morphologically normal and live increased significantly (P < 0.05) after 60 d of supplementation. In the control group, there were no changes in motility parameters while the concentration and total sperm count decreased over the duration of the study. In conclusion, supplementation with selenium and vitamin E for 60 d can improve the quality of semen in dogs with lowered fertility.

Plasma Hormone Levels and Semen Quality in Male Cats during Non-Breeding and Breeding Seasons

2009 ◽  
Vol 44 ◽  
pp. 291-293 ◽  
Author(s):  
T Tsutsui ◽  
F Onodera ◽  
H Oba ◽  
T Mizutani ◽  
T Hori
Keyword(s):  
Semen Quality ◽  
Hormone Levels ◽  
Plasma Hormone ◽  
Breeding Seasons

scholarly journals Effect of Rambutan Fruit Peel Extract on Total Sperm Counts of Wistar Rats With Obesity

Biota ◽  
2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Kerin Victoria Sipahutar ◽  
Yudhi Nugraha ◽  
Cut Fauziah
Keyword(s):  
Wistar Rats ◽  
Semen Quality ◽  
Sperm Count ◽  
Control Group ◽  
Antioxidant Compounds ◽  
P Value ◽  
High Fat ◽  
Fruit Peel ◽  
Group I ◽  
Peel Extract

Obesity caused by a high-fat diet leads to an altered reproductive hormonal profile, including impaired semen quality. Antioxidants can overcome these conditions. One of the well-known sources of antioxidants is in the rambutan fruit peel extract. This research aimed to figure the effect of rambutan fruit peel extract towards total sperm count in Wistar rats induced with high-fat feed. The study design used post-test only control group,  subjects were 30 male Wistar rats divided into five groups: Group I (Positive Control) was given high-fat feed, Group II (Treatment Control Group) was given 15mg/kg BW rambutan fruit peel extract, whereas group III, IV, V (Treatment Group) were given an extract of rambutan skin with a dosage of 15, 30, and 60 mg/kg BW, respectively. Treatment was administered for 81 days. This study showed that rambutan fruit peel extract with doses of 15, 30, and 60 mg/kg BW has significantly increased total sperm count in Wistar rats induced with high-fat feed. The outcome using Kruskal Wallis shows a result of p-value 0,010 (CI 95%). Antioxidant compounds found in rambutan fruit peel extract significantly increase total sperm count in Wistar rats induced with high-fat feed, with the highest efficacy on the dose of 15mg/kg BW.   Keywords: Fruit Peel Rambutan Extract; ; ; 

scholarly journals Effect of Apatyakara Ghrita Followed by Koshtha Shuddhi with Haritakyadi Churna in the Management of Ksheena Shukra w.s.r. to Oligozoospermia: A Case Study

AYUSHDHARA ◽  
2021 ◽  
pp. 3377-3383
Author(s):  
Devesh Jaiman ◽  
HML Meena ◽  
Bharat Kumar Padhar ◽  
Harish Bhakuni
Keyword(s):  
Male Infertility ◽  
Genetic Factors ◽  
Semen Quality ◽  
Sperm Count ◽  
Common Finding ◽  
Testicular Dysfunction ◽  
Seminal Parameters ◽  
Associated Symptoms

Oligozoospermia is a condition where sperm count is below 15 million/ml and a common finding which is related to male infertility. Some of the known responsible factors for male infertility are poor semen quality, genetic factors, testicular dysfunction and endocrine inter relationship etc. According to Ayurvedic classics, Oligozoospermia can be correlated with Ksheena Shukra which is due to increased Vata and Pitta Dosha being more prevalent in Madhyama Vayas. According to WHO, in India, the overall prevalence of primary infertility ranges between 3.9% and 16.8%.  Methods: In this study, a male patient aged 40 years was presented with a desire to get a child and other associated symptoms were Daurbalya (weakness), Mukha Shosha (dryness of the mouth), Sadana (loss of rigidity), Shrama (post act exhaustion), Alpachestata (early ejaculation), Maithune Ashakti (problematic or not satisfactory coitus), Rati Anabhimukhata (loss of sexual desire) etc. The patient was managed with Apatyakara Ghrita followed by Koshtha Shuddhi with Haritakyadi Churna. For the assessment of the effect of therapies on sexual parameters, the scoring pattern prepared by Mehra & Singh, 1995 and Seminal Parameters has been adopted. Results and Conclusion: Apatyakara Ghrita is found effective to increase sperm count from 12 million/ ml to 45 million/ml and motility from 20% to 75% and no adverse effect of the adopted management was seen during treatment and even after the 2 months of follow-up.

scholarly journals Low semen quality and adverse histological changes in testes of adult male mice treated with bee venom (Apis mellifera)

2021 ◽  
Vol 11 (1) ◽  
pp. 70-79
Author(s):  
Sassia O. Regeai ◽  
Salma A. Abusrer ◽  
Naema S. Shibani
Keyword(s):  
Male Infertility ◽  
Adult Male ◽  
Semen Quality ◽  
Sperm Count ◽  
Bee Venom ◽  
Reproductive Status ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Male Mice ◽  
Histological Changes

Background: Male infertility has been on the rise since the past seven decades. Recently, in Libya, bee venom therapy (BVT) has become a popular method among alternative healthcare practitioners for treating male infertility. However, a literature search did not find any published studies that investigated the use of BVT for infertility treatment. Aim: To investigate the effect of bee venom on the male reproductive status through measurements of semen quality parameters and testicular histological changes in adult male mice. Methods: A total of 48 male mice were randomly divided into three experimental groups (which were subdivided into two subgroups with eight mice each) as follows: control, bee venom sting (BVS), and bee venom injection (BVI). The normal control subgroup mice were not subjected to any treatment, while the vehicle control subgroup mice were injected (i.p.) with 200 μl of 0.9% saline solution. In the BVS-treated subgroups, each mouse was stung by one live bee for five times (BVS-5) or seven times (BVS-7) every third day for 2 or 3 weeks. While each mouse in the BVI-treated subgroups received 23 μg/kg in a dose volume of 200 μl BVIs (i.p.) for five times (BVI-5) or seven times (BVI-7) every third day for 15 or 21 days. Results: The findings of this study showed that repeated bee venom treatment by sting or injection to adult male mice resulted in a significant decline in testosterone levels, sperm count, sperm motility, and a very significant increase in the percentage of abnormal sperm morphology; also, there were harmful testicular histological changes in the structural organization of seminiferous tubules and degenerative changes in the germinal epithelium compared to control group. Conclusion: The results of this study provide evidence for the low semen quality and adverse testicular histological changes in male mice treated with bee venom. Hence, there is a desperate need for educating alternative healthcare practitioners and infertile couples about the harmful effects of BVT on reproductive status.

scholarly journals Comparison of mice’ sperm parameters exposed to some hazardous physical agents

2021 ◽  
Vol 36 (3) ◽  
pp. e2021013
Author(s):  
Mohammad-Bagher Abdollahi ◽  
Somayeh Farhang Dehghan ◽  
Faezeh Abasi Balochkhaneh ◽  
Manouchehr Ahmadi Moghadam ◽  
Hamzeh Mohammadi
Keyword(s):  
Sperm Count ◽  
Semen Analysis ◽  
Field Studies ◽  
Sperm Parameters ◽  
World Health ◽  
Control Group ◽  
Normal Morphology ◽  
Progressive Motility ◽  
Noise Vibration ◽  
Health Organization

The present study was aimed to compare the effects of exposure to noise, vibration, lighting, and microwave on male mice’ sperm parameters. The mice were randomly assigned to five groups of eight, which comprised of the unexposed group and exposure groups including the lighting (1000 lux), noise (100 dB(A)), vibration (acceleration of 1.2 m/s2) and microwave (power density of 5 watts). The exposure groups were subjected to the four agents for 8 hours a day, 5 days a week during a 2-week period. Semen analysis were done according to World Health Organization guidelines. The highest significant mean difference in sperm count (-1.35×106/mL) had being observed between the microwave group and the control one (P=0.001). The highest difference in immotile percent (25.88 %) had being observed between the noise group and the control one (P=0.001). The highest difference in normal morphology (-27.06 %) observed between the lighting exposure group and the control group (P=0.001). The four agents can cause changes in different sperm parameters, however for definite conclusion; more laboratory and field studies are required. In total, exposure to microwave has had the greatest effect on sperm count and exposure to light has had the greatest effect on normal morphology and non-progressive motility. Moreover, exposure to noise has had the greatest effect on progressive motility and immotile percent, respectively.

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