scholarly journals Quantitative ultrasound imaging of therapy response in bladder cancer in vivo

Oncoscience ◽  
2016 ◽  
Vol 3 (3-4) ◽  
pp. 122-133 ◽  
Author(s):  
William T. Tran ◽  
Lakshmanan Sannachi ◽  
Naum Papanicolau ◽  
Hadi Tadayyon ◽  
Azza Al Mahrouki ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Ultrasound Imaging ◽  
Quantitative Ultrasound ◽  
Therapy Response

scholarly journals Molecular Ultrasound Imaging

Nanomaterials ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1935 ◽  
Author(s):  
Gurbet Köse ◽  
Milita Darguzyte ◽  
Fabian Kiessling
Keyword(s):  
Ultrasound Imaging ◽  
Therapy Response ◽  
Radiation Force ◽  
Liver Lesion ◽  
Clinical Translation ◽  
Response Monitoring ◽  
Increased Sensitivity ◽  
Molecular Ultrasound ◽  
Target Binding

In the last decade, molecular ultrasound imaging has been rapidly progressing. It has proven promising to diagnose angiogenesis, inflammation, and thrombosis, and many intravascular targets, such as VEGFR2, integrins, and selectins, have been successfully visualized in vivo. Furthermore, pre-clinical studies demonstrated that molecular ultrasound increased sensitivity and specificity in disease detection, classification, and therapy response monitoring compared to current clinically applied ultrasound technologies. Several techniques were developed to detect target-bound microbubbles comprising sensitive particle acoustic quantification (SPAQ), destruction-replenishment analysis, and dwelling time assessment. Moreover, some groups tried to assess microbubble binding by a change in their echogenicity after target binding. These techniques can be complemented by radiation force ultrasound improving target binding by pushing microbubbles to vessel walls. Two targeted microbubble formulations are already in clinical trials for tumor detection and liver lesion characterization, and further clinical scale targeted microbubbles are prepared for clinical translation. The recent enormous progress in the field of molecular ultrasound imaging is summarized in this review article by introducing the most relevant detection technologies, concepts for targeted nano- and micro-bubbles, as well as their applications to characterize various diseases. Finally, progress in clinical translation is highlighted, and roadblocks are discussed that currently slow the clinical translation.

In vivo performance of a matrix-based quantitative ultrasound imaging device dedicated to calcaneus investigation

2002 ◽  
Vol 28 (10) ◽  
pp. 1285-1293 ◽  
Author(s):  
M.A Gomez ◽  
M Defontaine ◽  
B Giraudeau ◽  
E Camus ◽  
L Colin ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Quantitative Ultrasound ◽  
Imaging Device

In vivo quantitative ultrasound imaging of peripheral bones with a new device

1996 ◽  
Vol 6 (S1) ◽  
pp. 174-174
Author(s):  
P Laugier ◽  
B Fournier ◽  
P Giat ◽  
G Berger
Keyword(s):  
Ultrasound Imaging ◽  
Quantitative Ultrasound ◽  
New Device

Quantitative ultrasound imaging to monitor in vivo high-intensity ultrasound treatment

2014 ◽  
Vol 136 (4) ◽  
pp. 2125-2125
Author(s):  
Goutam Ghoshal ◽  
Jeremy P. Kemmerer ◽  
Chandra Karunakaran ◽  
Rami Abuhabshah ◽  
Rita J. Miller ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Quantitative Ultrasound ◽  
High Intensity ◽  
Ultrasound Treatment ◽  
High Intensity Ultrasound

Low-frequency quantitative ultrasound imaging of cell death in vivo

2013 ◽  
Vol 40 (8) ◽  
pp. 082901 ◽  
Author(s):  
Ali Sadeghi-Naini ◽  
Naum Papanicolau ◽  
Omar Falou ◽  
Hadi Tadayyon ◽  
Justin Lee ◽  
...  
Keyword(s):  
Cell Death ◽  
Ultrasound Imaging ◽  
Quantitative Ultrasound ◽  
Low Frequency

Surface Labeling with Adhesion Protein FimH Improves Binding of Immunotherapeutic Agent Salmonella Ty21a to the Bladder Epithelium

2020 ◽  
pp. 1-12
Author(s):  
Maroeska J. Burggraaf ◽  
Lisette Waanders ◽  
Mariska Verlaan ◽  
Janneke Maaskant ◽  
Diane Houben ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Antitumor Activity ◽  
Bladder Wall ◽  
Bladder Epithelium ◽  
Modest Improvement ◽  
Adhesion Protein ◽  
Survival Experiment ◽  
Muscle Invasive

BACKGROUND: Bladder cancer is the ninth most common cancer in men. 70% of these tumors are classified as non-muscle invasive bladder cancer and those patients receive 6 intravesical instillations with Mycobacterium bovis BCG after transurethral resection. However, 30% of patients show recurrences after treatment and experience severe side effects that often lead to therapy discontinuation. Recently, another vaccine strain, Salmonella enterica typhi Ty21a, demonstrated promising antitumor activity in vivo. Here we focus on increasing bacterial retention in the bladder in order to reduce the number of instillations required and improve antitumor activity. OBJECTIVE: To increase the binding of Ty21a to the bladder wall by surface labeling of the bacteria with adhesion protein FimH and to study its effect in a bladder cancer mouse model. METHODS: Binding of Ty21a with surface-labeled FimH to the bladder wall was analyzed in vitro and in vivo. The antitumor effect of a single instillation of Ty21a+FimH in treatment was determined in a survival experiment. RESULTS: FimH-labeled Ty21a showed significant (p <  0.0001) improved binding to mouse and human cell lines in vitro. Furthermore, FimH labeled bacteria showed ∼5x more binding to the bladder than controls in vivo. Enhanced binding to the bladder via FimH labeling induced a modest improvement in median but not in overall mice survival. CONCLUSIONS: FimH labeling of Ty21a significantly improved binding to bladder tumor cells in vitro and the bladder wall in vivo. The improved binding leads to a modest increase in median survival in a single bladder cancer mouse study.

scholarly journals RBMX suppresses tumorigenicity and progression of bladder cancer by interacting with the hnRNP A1 protein to regulate PKM alternative splicing

Oncogene ◽  
2021 ◽  
Author(s):  
Qiuxia Yan ◽  
Peng Zeng ◽  
Xiuqin Zhou ◽  
Xiaoying Zhao ◽  
Runqiang Chen ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Alternative Splicing ◽  
Rna Binding ◽  
Aerobic Glycolysis ◽  
Histological Grade ◽  
Migration And Invasion ◽  
Binding Motif ◽  
Hnrnp A1

AbstractThe prognosis for patients with metastatic bladder cancer (BCa) is poor, and it is not improved by current treatments. RNA-binding motif protein X-linked (RBMX) are involved in the regulation of the malignant progression of various tumors. However, the role of RBMX in BCa tumorigenicity and progression remains unclear. In this study, we found that RBMX was significantly downregulated in BCa tissues, especially in muscle-invasive BCa tissues. RBMX expression was negatively correlated with tumor stage, histological grade and poor patient prognosis. Functional assays demonstrated that RBMX inhibited BCa cell proliferation, colony formation, migration, and invasion in vitro and suppressed tumor growth and metastasis in vivo. Mechanistic investigations revealed that hnRNP A1 was an RBMX-binding protein. RBMX competitively inhibited the combination of the RGG motif in hnRNP A1 and the sequences flanking PKM exon 9, leading to the formation of lower PKM2 and higher PKM1 levels, which attenuated the tumorigenicity and progression of BCa. Moreover, RBMX inhibited aerobic glycolysis through hnRNP A1-dependent PKM alternative splicing and counteracted the PKM2 overexpression-induced aggressive phenotype of the BCa cells. In conclusion, our findings indicate that RBMX suppresses BCa tumorigenicity and progression via an hnRNP A1-mediated PKM alternative splicing mechanism. RBMX may serve as a novel prognostic biomarker for clinical intervention in BCa.

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