Low-frequency quantitative ultrasound imaging of cell death in vivo

2013 ◽  
Vol 40 (8) ◽  
pp. 082901 ◽  
Author(s):  
Ali Sadeghi-Naini ◽  
Naum Papanicolau ◽  
Omar Falou ◽  
Hadi Tadayyon ◽  
Justin Lee ◽  
...  
Keyword(s):  
Cell Death ◽  
Ultrasound Imaging ◽  
Quantitative Ultrasound ◽  
Low Frequency

27. Low Frequency Ultrasound Imaging of Apoptosis in Tumor Response: Non-Invasive Monitoring of Chemotherapy Effects

2018 ◽  
Author(s):  
Stephanie Zhou
Keyword(s):  
Cell Death ◽  
Ultrasound Imaging ◽  
Structural Changes ◽  
Imaging Techniques ◽  
Low Frequency ◽  
Acoustic Properties ◽  
High Frequency Ultrasound ◽  
Noninvasive Methods ◽  
Frequency Ultrasound

Due to the growing costs of chemotherapy, previous imaging techniques such as MRI or CT scans have become too time-consuming in the assessment of chemotherapy’s effects. With results generated about 2 weeks later, the patient is exposed to the negative side effects of these medications with the possibility that chemotherapy may not be improving their prognosis. Thus, ultrasound has become increasingly popular as a method to determine chemotherapy’s effect on tumors within 24 hours. Both low and high-frequency ultrasound are novel, noninvasive methods for detecting cell death based on changes in cell morphology. Condensation, fragmentation and alterations in the cell nucleus during apoptosis are linked to changes in the cell’s acoustic properties, as indicated by experimental evidence. In this study, quantitative ultrasound was used to follow responses of tumor models to chemotherapy in vivo. As studies have shown that structural changes can occur as early as 24 hours after treatment, ultrasound imaging was administered before and 24 hours after treatment. Changes in ultrasound parameters such as spectral slope, Y-intercept, and midband fit were analyzed relative to pretreatment control data and when compared to changes in the tumors seen through cell staining, changes consistent with cell death were observed.

scholarly journals Ultrasound imaging of apoptosis: high-resolution non-invasive monitoring of programmed cell death in vitro, in situ and in vivo

1999 ◽  
Vol 81 (3) ◽  
pp. 520-527 ◽  
Author(s):  
G J Czarnota ◽  
M C Kolios ◽  
J Abraham ◽  
M Portnoy ◽  
F P Ottensmeyer ◽  
...  
Keyword(s):  
Cell Death ◽  
High Resolution ◽  
Programmed Cell Death ◽  
Ultrasound Imaging ◽  
Invasive Monitoring ◽  
Non Invasive

In vivo performance of a matrix-based quantitative ultrasound imaging device dedicated to calcaneus investigation

2002 ◽  
Vol 28 (10) ◽  
pp. 1285-1293 ◽  
Author(s):  
M.A Gomez ◽  
M Defontaine ◽  
B Giraudeau ◽  
E Camus ◽  
L Colin ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Quantitative Ultrasound ◽  
Imaging Device

scholarly journals From high-frequency to low-frequency cell death detection: quantitative ultrasound evaluation of tumor response in breast cancer

2013 ◽  
Author(s):  
Gregory J. Czarnota ◽  
Ali Sadeghi-Naini ◽  
Naum Papanicolau ◽  
Omar Falou ◽  
Rebecca Dent ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Death ◽  
Quantitative Ultrasound ◽  
High Frequency ◽  
Tumor Response ◽  
Low Frequency ◽  
Ultrasound Evaluation

scholarly journals Quantitative ultrasound imaging of therapy response in bladder cancer in vivo

Oncoscience ◽  
2016 ◽  
Vol 3 (3-4) ◽  
pp. 122-133 ◽  
Author(s):  
William T. Tran ◽  
Lakshmanan Sannachi ◽  
Naum Papanicolau ◽  
Hadi Tadayyon ◽  
Azza Al Mahrouki ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Ultrasound Imaging ◽  
Quantitative Ultrasound ◽  
Therapy Response

Ultrasound Biomicroscopy of Cancer Therapy Effects: Correlation between Light and Electron Microscopy, and a New Non-Invasive Ultrasound Imaging Method for Detecting Apoptosis

2000 ◽  
Vol 6 (S2) ◽  
pp. 1014-1015
Author(s):  
G.J. Czarnota ◽  
M.C. Kolios ◽  
Y.M. Heng ◽  
K. Devaraj ◽  
C. Tam ◽  
...  
Keyword(s):  
Cell Death ◽  
Photodynamic Therapy ◽  
Ultrasound Imaging ◽  
Light And Electron Microscopy ◽  
Ultrasound Biomicroscopy ◽  
Response To Treatment ◽  
Imaging Method ◽  
High Frequency Ultrasound

We have discovered that high-frequency ultrasound imaging, or ultrasound biomicroscopy, can be used to detect apoptosis in a number of experimental systems. We have shown that such detection with 30-40 MHz ultrasound is possible using cells in an in vitro system (AML-3 leukemia cells) made to undergo apoptosis in response to treatment with a variety of cancer killing chemotherapeutic drugs. We have shown that ultrasound biomicroscopy can also detect programmed cell death in tissues made to undergo apoptosis in response to photodynamic therapy, currently an experimental cancer treating regimen. Lastly, we have shown that this ultrasound imaging approach works in vivo, using living animals where apoptosis has been induced similarly using photodynamic therapy. Specifically, apoptotic cells and regions of apoptosis in tissues exhibit up to a 36-fold increase in ultrasound backscatter intensity permitting this type of cell death to be readily discriminated from surrounding viable tissue.

In vivo quantitative ultrasound imaging of peripheral bones with a new device

1996 ◽  
Vol 6 (S1) ◽  
pp. 174-174
Author(s):  
P Laugier ◽  
B Fournier ◽  
P Giat ◽  
G Berger
Keyword(s):  
Ultrasound Imaging ◽  
Quantitative Ultrasound ◽  
New Device

Quantitative ultrasound imaging to monitor in vivo high-intensity ultrasound treatment

2014 ◽  
Vol 136 (4) ◽  
pp. 2125-2125
Author(s):  
Goutam Ghoshal ◽  
Jeremy P. Kemmerer ◽  
Chandra Karunakaran ◽  
Rami Abuhabshah ◽  
Rita J. Miller ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Quantitative Ultrasound ◽  
High Intensity ◽  
Ultrasound Treatment ◽  
High Intensity Ultrasound
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