scholarly journals The Tape-Stripping Technique as a Method for Drug Quantification in Skin

2008 ◽  
Vol 11 (1) ◽  
pp. 104 ◽  
Author(s):  
Jose Juan Escobar-Chavez ◽  
V. Merino-Sanjuán ◽  
M. López-Cervantes ◽  
Z. Urban-Morlan ◽  
E. Piñón-Segundo ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Penetration Depth ◽  
Transdermal Delivery ◽  
Tape Stripping ◽  
Drug Penetration ◽  
Human Stratum Corneum ◽  
Investigative Techniques

Quantification of drugs within the skin is essential for topical and transdermal delivery research. Over the last two decades, horizontal sectioning, consisting of tape stripping throughout the stratum corneum, has become one of the traditional investigative techniques. Tape stripping of human stratum corneum is widely used as a method for studying the kinetics and penetration depth of drugs. This paper shows the applications of the tape stripping technique to quantify drug penetration through the skin, underlining its versatile application in the area of topical and transdermal drugs.

scholarly journals Effect of Modulated Alternating and Direct Current Iontophoresis on Transdermal Delivery of Lidocaine Hydrochloride

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Gaurav Bhatia ◽  
Ajay K. Banga
Keyword(s):  
Stratum Corneum ◽  
Direct Current ◽  
Transdermal Delivery ◽  
Alternating Current ◽  
Lidocaine Hydrochloride ◽  
Tape Stripping ◽  
Porcine Skin ◽  
Skin Receptor ◽  
Time Periods ◽  
Iontophoretic Delivery

The objective of this study was to investigate the iontophoretic delivery of lidocaine hydrochloride through porcine skin and to compare the effects of modulated alternating and direct current iontophoresis. Continuous and modulated iontophoresis was applied for one hour and two hours (0-1 h and 4-5th h) using a 1% w/v solution of lidocaine hydrochloride. Tape stripping was done to quantify the amount of drug permeated into stratum corneum and skin extraction studies were performed to determine the amount of drug in stripped skin. Receptor was sampled and analyzed over predefined time periods. The amount of lidocaine delivered across porcine skin after modulated direct current iontophoresis for 2 h was1069.87±120.03 μg/sq·cm compared to744.81±125.41 μg/sq·cm after modulated alternating current iontophoresis for 2 h. Modulated direct current iontophoresis also enhanced lidocaine delivery by twelvefold compared to passive delivery as91.27±18.71 μg/sq·cm of lidocaine was delivered after passive delivery. Modulated iontophoresis enhanced the delivery of lidocaine hydrochloride across porcine skin compared to the passive delivery. Modulated alternating current iontophoresis for duration of 2 h at frequency of 1 kHz was found to be comparable to the continuous direct current iontophoresis for 1 h.

Tape stripping of human stratum corneum yields cell layers that originate from various depths because of furrows in the skin

1997 ◽  
Vol 289 (9) ◽  
pp. 514-518 ◽  
Author(s):  
R. G. van der Molen ◽  
F. Spies ◽  
J. M. van 't Noordende ◽  
E. Boelsma ◽  
A. M. Mommaas ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Tape Stripping ◽  
Human Stratum Corneum ◽  
Cell Layers

Use of the Tape Stripping Technique for Directly Quantifying Esterase Activities in Human Stratum Corneum

2001 ◽  
Vol 290 (2) ◽  
pp. 179-185 ◽  
Author(s):  
Frédéric Beisson ◽  
Mustapha Aoubala ◽  
Sylvie Marull ◽  
Anne-Marie Moustacas-Gardies ◽  
Robert Voultoury ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Tape Stripping ◽  
Human Stratum Corneum ◽  
Esterase Activities

scholarly journals In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations

2014 ◽  
Vol 64 (2) ◽  
pp. 257-265 ◽  
Author(s):  
Santo Scalia ◽  
Valentina Trotta ◽  
Anna Bianchi
Keyword(s):  
Stratum Corneum ◽  
Skin Penetration ◽  
Tape Stripping ◽  
Human Stratum Corneum ◽  
Percutaneous Permeation ◽  
Oil In Water ◽  
Human Volunteers ◽  
Model Oil ◽  
Gel Formulations

Abstract The aim of the study was to examine the effect of topical vehicles on the in vivo human stratum corneum penetration of the antioxidant and skin photoprotective agent (-)-epigallocatechin-3-gallate (EGCG). Model oil-in-water (o/w) emulsion and gel formulations containing 1 % (m/m) EGCG were prepared and subjected to photodegradation studies in order to select excipients that minimize the light instability of EGCG. The optimized emulsion and gel were applied to human volunteers and the EGCG percutaneous permeation was evaluated in vivo by the tape- -stripping technique. No significant differences in the percentage of the applied EGCG dose diffused into the stratum corneum were observed between the o/w emulsion (36.1 ± 7.5 %) and gel (35.5 ± 8.1 %) preparations. However, the amount of EGCG permeated into the deeper region of human stratum corneum was significantly larger for the o/w emulsion compared to the gel. Therefore, the emulsion represents a suitable vehicle for topical delivery of EGCG.

Identifying underlying issues related to the inactive excipients of transfersomes based drug delivery system

2020 ◽  
Vol 26 ◽  
Author(s):  
Drashti Patel ◽  
Bappaditya Chatterjee
Keyword(s):  
Drug Delivery ◽  
Drug Delivery System ◽  
Delivery System ◽  
Transdermal Delivery ◽  
Systemic Availability ◽  
Drug Penetration ◽  
Skin Damage ◽  
Variable Effect ◽  
Practical Factors ◽  
Edge Activator

: Transfersomes are bilayer vesicles composed of phospholipid and edge-activators, which are mostly surfactant. Transfersomes based drug delivery system has gained a lot of interest of the pharmaceutical researchers for their ability to improve drug penetration and permeation through the skin. Transdermal drug delivery via transfersomes has the potential to overcome the challenge of low systemic availability. However, this complex vesicular system has different issues to consider for developing a successful transdermal delivery system. One of the major ingredients, phospholipid has versatile sources and variable effect on the vesicle size and drug entrapment in transfersomes. The other one termed as edge-activator or surfactant has some crucial consideration of skin damage and toxicity depending upon its type and concentration. A complex interaction between type and concentration of phospholipid and surfactant was observed, which affect the physicochemical properties of transfersomes. This review focuses on the practical factors related to these two major ingredients such as phospholipid and surfactant. The origin, purity, desired concentration, the susceptibility of degradation, etc. are the important factors for selecting phospholipid. Regarding surfactants, the major aspects are type and desired concentration. A successful development of transfersomes based drug delivery system depends on the proper considerations of these factors and practical aspects.

scholarly journals Size-Tunable Paclitaxel Nanoparticles Stabilized by Anionic Phospholipids for Transdermal Delivery Applications

2020 ◽  
Vol 15 (3) ◽  
pp. 1934578X1990068
Author(s):  
Noriyuki Uchida ◽  
Masayoshi Yanagi ◽  
Hiroki Hamada
Keyword(s):  
Stratum Corneum ◽  
Transdermal Delivery ◽  
Skin Tissue ◽  
Composite Nanoparticles ◽  
Rat Skin ◽  
Cooling Process ◽  
Anionic Phospholipid ◽  
Anionic Phospholipids ◽  
Subsequent Heating

Composite nanoparticles composed of an anionic phospholipid of 1,2-dipalmitoyl-sn-glycero-3-phosphorylglycerol (DPPG) and paclitaxel (PTX) were successfully prepared by mixing them in water followed by a subsequent heating/cooling process. The size of DPPG-PTX nanoparticle could be easily tuned by ultrasonic fragmentation. Upon addition of small-sized fluorescently labeled paclitaxel (FLPTX) nanoparticles with DPPG (DPPG-FLPTX) to rat skin tissue, part of the FLPTX molecules permeated to the stratum corneum.

Sign in / Sign up

Export Citation Format

Share Document