Use of the Tape Stripping Technique for Directly Quantifying Esterase Activities in Human Stratum Corneum

2001 ◽  
Vol 290 (2) ◽  
pp. 179-185 ◽  
Author(s):  
Frédéric Beisson ◽  
Mustapha Aoubala ◽  
Sylvie Marull ◽  
Anne-Marie Moustacas-Gardies ◽  
Robert Voultoury ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Tape Stripping ◽  
Human Stratum Corneum ◽  
Esterase Activities

Tape stripping of human stratum corneum yields cell layers that originate from various depths because of furrows in the skin

1997 ◽  
Vol 289 (9) ◽  
pp. 514-518 ◽  
Author(s):  
R. G. van der Molen ◽  
F. Spies ◽  
J. M. van 't Noordende ◽  
E. Boelsma ◽  
A. M. Mommaas ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Tape Stripping ◽  
Human Stratum Corneum ◽  
Cell Layers

scholarly journals The Tape-Stripping Technique as a Method for Drug Quantification in Skin

2008 ◽  
Vol 11 (1) ◽  
pp. 104 ◽  
Author(s):  
Jose Juan Escobar-Chavez ◽  
V. Merino-Sanjuán ◽  
M. López-Cervantes ◽  
Z. Urban-Morlan ◽  
E. Piñón-Segundo ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Penetration Depth ◽  
Transdermal Delivery ◽  
Tape Stripping ◽  
Drug Penetration ◽  
Human Stratum Corneum ◽  
Investigative Techniques

Quantification of drugs within the skin is essential for topical and transdermal delivery research. Over the last two decades, horizontal sectioning, consisting of tape stripping throughout the stratum corneum, has become one of the traditional investigative techniques. Tape stripping of human stratum corneum is widely used as a method for studying the kinetics and penetration depth of drugs. This paper shows the applications of the tape stripping technique to quantify drug penetration through the skin, underlining its versatile application in the area of topical and transdermal drugs.

scholarly journals In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations

2014 ◽  
Vol 64 (2) ◽  
pp. 257-265 ◽  
Author(s):  
Santo Scalia ◽  
Valentina Trotta ◽  
Anna Bianchi
Keyword(s):  
Stratum Corneum ◽  
Skin Penetration ◽  
Tape Stripping ◽  
Human Stratum Corneum ◽  
Percutaneous Permeation ◽  
Oil In Water ◽  
Human Volunteers ◽  
Model Oil ◽  
Gel Formulations

Abstract The aim of the study was to examine the effect of topical vehicles on the in vivo human stratum corneum penetration of the antioxidant and skin photoprotective agent (-)-epigallocatechin-3-gallate (EGCG). Model oil-in-water (o/w) emulsion and gel formulations containing 1 % (m/m) EGCG were prepared and subjected to photodegradation studies in order to select excipients that minimize the light instability of EGCG. The optimized emulsion and gel were applied to human volunteers and the EGCG percutaneous permeation was evaluated in vivo by the tape- -stripping technique. No significant differences in the percentage of the applied EGCG dose diffused into the stratum corneum were observed between the o/w emulsion (36.1 ± 7.5 %) and gel (35.5 ± 8.1 %) preparations. However, the amount of EGCG permeated into the deeper region of human stratum corneum was significantly larger for the o/w emulsion compared to the gel. Therefore, the emulsion represents a suitable vehicle for topical delivery of EGCG.

Thermal Behaviour of Human Stratum Corneum

2006 ◽  
Vol 19 (3) ◽  
pp. 132-139 ◽  
Author(s):  
C.L. Silva ◽  
S.C.C. Nunes ◽  
M.E.S. Eusébio ◽  
A.A.C.C. Pais ◽  
J.J.S. Sousa
Keyword(s):  
Stratum Corneum ◽  
Thermal Behaviour ◽  
Human Stratum Corneum
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