scholarly journals Biotransformation of phthalates by basidiomycete fungi from different ecophysiological groups

2021 ◽  
Vol 1 (Supplement) ◽  
Author(s):  
T. Fedorova ◽  
K. Moiseenko ◽  
O. Savinova ◽  
O. Glazunova ◽  
S. Eremin
Keyword(s):  
Basidiomycete Fungi

Production and Localization of Proteinases in Colonies of Timber-decaying Basidiomycete Fungi

Microbiology ◽  
1989 ◽  
Vol 135 (5) ◽  
pp. 1369-1374
Author(s):  
C. E. Venables ◽  
S. C. Watkinson
Keyword(s):  
Basidiomycete Fungi

scholarly journals Protein Engineering Approaches to Enhance Fungal Laccase Production in S. cerevisiae

2021 ◽  
Vol 22 (3) ◽  
pp. 1157
Author(s):  
Pablo Aza ◽  
Felipe de Salas ◽  
Gonzalo Molpeceres ◽  
David Rodríguez-Escribano ◽  
Iñigo de la Fuente ◽  
...  
Keyword(s):  
Protein Engineering ◽  
Directed Evolution ◽  
Signal Peptide ◽  
Laccase Activity ◽  
Laccase Production ◽  
Wide Range ◽  
Mating Factor ◽  
Conserved Amino Acids ◽  
Basidiomycete Fungi

Laccases secreted by saprotrophic basidiomycete fungi are versatile biocatalysts able to oxidize a wide range of aromatic compounds using oxygen as the sole requirement. Saccharomyces cerevisiae is a preferred host for engineering fungal laccases. To assist the difficult secretion of active enzymes by yeast, the native signal peptide is usually replaced by the preproleader of S. cerevisiae alfa mating factor (MFα1). However, in most cases, only basal enzyme levels are obtained. During directed evolution in S. cerevisiae of laccases fused to the α-factor preproleader, we demonstrated that mutations accumulated in the signal peptide notably raised enzyme secretion. Here we describe different protein engineering approaches carried out to enhance the laccase activity detected in the liquid extracts of S. cerevisiae cultures. We demonstrate the improved secretion of native and engineered laccases by using the fittest mutated α-factor preproleader obtained through successive laccase evolution campaigns in our lab. Special attention is also paid to the role of protein N-glycosylation in laccase production and properties, and to the introduction of conserved amino acids through consensus design enabling the expression of certain laccases otherwise not produced by the yeast. Finally, we revise the contribution of mutations accumulated in laccase coding sequence (CDS) during previous directed evolution campaigns that facilitate enzyme production.

Filamentous ascomycete and basidiomycete fungi from beach sand

2016 ◽  
Vol 27 (4) ◽  
pp. 603-607 ◽  
Author(s):  
Teh Li Yee ◽  
Rosnida Tajuddin ◽  
Nik Mohd Izham Mohamed Nor ◽  
Masratul Hawa Mohd ◽  
Latiffah Zakaria
Keyword(s):  
Beach Sand ◽  
Filamentous Ascomycete ◽  
Basidiomycete Fungi

scholarly journals Nuclear selection is effectively policed by mating restrictions of the dikaryotic life cycle of mushroom-forming fungi

2020 ◽  
Author(s):  
Ben Auxier ◽  
Tamas Czaran ◽  
Duur Aanen
Keyword(s):  
Life Cycle ◽  
Nuclear Envelope ◽  
Social Interactions ◽  
Haploid Nucleus ◽  
Fitness Consequences ◽  
Nuclear Selection ◽  
Related Individuals ◽  
Nuclear Exchange ◽  
Basidiomycete Fungi

AbstractAltruistic social interactions generally evolve between genetically related individuals or other replicators, whereas sexual interactions usually occur among unrelated individuals. This tension between social and sexual interactions is resolved by policing mechanisms enforcing cooperation among genetically unrelated entities. For example, most organisms with two haploid genomes are diploid, both genomes encapsulated inside a single nuclear envelope. A fascinating exception to this are Basidiomycete fungi, where the two haploid genomes remain separate. Uniquely, the haploid nuclei of the dikaryon can fertilize subsequent gametes encountered, the presumed benefit of this lifecycle. The implications for the balance of selection within and among individuals are largely unexplored. We modelled the implications of a fitness tradeoff at the level of the haploid nucleus versus the level of the fungal individual. We show that the most important policing mechanism is prohibition of fusion between dikaryons, which can otherwise select for detrimental levels of nuclear mating fitness. An additional policing mechanism revealed by our model is linkage between loci with fitness consequences. Our results show that benefits of di-mon matings must be paired with policing mechanisms to avoid uncontrolled selection at the level of the nuclei. Furthermore, we discuss evolutionary implications of recent claims of nuclear exchange in related fungal groups.

scholarly journals Role of basidiomycete fungi in the grapevine trunk disease esca

2019 ◽  
Vol 69 (2) ◽  
pp. 205-220 ◽  
Author(s):  
A. A. Brown ◽  
D. P. Lawrence ◽  
K. Baumgartner
Keyword(s):  
Trunk Disease ◽  
Grapevine Trunk Disease ◽  
Basidiomycete Fungi

scholarly journals Ascomycete Aspergillus oryzae Is an Efficient Expression Host for Production of Basidiomycete Terpenes by Using Genomic DNA Sequences

2019 ◽  
Vol 85 (15) ◽  
Author(s):  
Shota Nagamine ◽  
Chengwei Liu ◽  
Jumpei Nishishita ◽  
Takuto Kozaki ◽  
Kaho Sogahata ◽  
...  
Keyword(s):  
Natural Products ◽  
Aspergillus Oryzae ◽  
Dna Sequences ◽  
Genomic Dna ◽  
Genome Mining ◽  
Biologically Active ◽  
Biosynthetic Genes ◽  
Content Type ◽  
Content System ◽  
Basidiomycete Fungi

ABSTRACT Basidiomycete fungi are an attractive resource for biologically active natural products for use in pharmaceutically relevant compounds. Recently, genome projects on mushroom fungi have provided a great deal of biosynthetic gene cluster information. However, functional analyses of the gene clusters for natural products were largely unexplored because of the difficulty of cDNA preparation and lack of gene manipulation tools for basidiomycete fungi. To develop a versatile host for basidiomycete genes, we examined gene expression using genomic DNA sequences in the robust ascomycete host Aspergillus oryzae, which is frequently used for the production of metabolites from filamentous fungi. Exhaustive expression of 30 terpene synthase genes from the basidiomycetes Clitopilus pseudo-pinsitus and Stereum hirsutum showed two splicing patterns, i.e., completely spliced cDNAs giving terpenes (15 cases) and mostly spliced cDNAs, indicating that A. oryzae correctly spliced most introns at the predicted positions and lengths. The mostly spliced cDNAs were expressed after PCR-based removal of introns, resulting in the successful production of terpenes (14 cases). During this study, we observed relatively frequent mispredictions in the automated program. Hence, the complementary use of A. oryzae expression and automated prediction will be a powerful tool for genome mining. IMPORTANCE The recent large influx of genome sequences from basidiomycetes, which are prolific producers of bioactive natural products, may provide opportunities to develop novel drug candidates. The development of a reliable expression system is essential for the genome mining of natural products because of the lack of a tractable host for heterologous expression of basidiomycete genes. For this purpose, we applied the ascomycete Aspergillus oryzae system for the direct expression of fungal natural product biosynthetic genes from genomic DNA. Using this system, 29 sesquiterpene synthase genes and diterpene biosynthetic genes for bioactive pleuromutilin were successfully expressed. Together with the use of computational tools for intron prediction, this Aspergillus oryzae system represents a practical method for the production of basidiomycete natural products.

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