scholarly journals Aquaporin-1 down regulation associated with inhibiting cell viability and inducing apoptosis of human lens epithelial cells

2016 ◽  
Vol 9 (1) ◽  
Keyword(s):  
Epithelial Cells ◽  
Cell Viability ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Down Regulation ◽  
Aquaporin 1 ◽  
Human Lens Epithelial Cells

scholarly journals Thioredoxin Binding Protein-2 Regulates Autophagy of Human Lens Epithelial Cells under Oxidative Stress via Inhibition of Akt Phosphorylation

2016 ◽  
Vol 2016 ◽  
pp. 1-17 ◽  
Author(s):  
Jiaojie Zhou ◽  
Ke Yao ◽  
Yidong Zhang ◽  
Guangdi Chen ◽  
Kairan Lai ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Cell Viability ◽  
Binding Protein ◽  
Negative Regulator ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells ◽  
Age Related

Oxidative stress plays an essential role in the development of age-related cataract. Thioredoxin binding protein-2 (TBP-2) is a negative regulator of thioredoxin (Trx), which deteriorates cellular antioxidant system. Our study focused on the autophagy-regulating effect of TBP-2 under oxidative stress in human lens epithelial cells (LECs). Human lens epithelial cells were used for cell culture and treatment. Lentiviral-based transfection system was used for overexpression of TBP-2. Cytotoxicity assay, western blot analysis, GFP/mCherry-fused LC3 plasmid, immunofluorescence, and transmission electronic microscopy were performed. The results showed that autophagic response of LECs with increased LC3-II, p62, and GFP/mCherry-LC3 puncta (P<0.01) was induced by oxidative stress. Overexpression of TBP-2 further strengthens this response and worsens the cell viability (P<0.01). Knockdown of TBP-2 attenuates the autophagic response and cell viability loss induced by oxidative stress. TBP-2 mainly regulates autophagy in the initiation stage, which is mTOR-independent and probably caused by the dephosphorylation of Akt under oxidative stress. These findings suggest a novel role of TBP-2 in human LECs under oxidative stress. Oxidative stress can cause cell injury and autophagy in LECs, and TBP-2 regulates this response. Hence, this study provides evidence regarding the role of TBP-2 in lens and the possible mechanism of cataract development.

Phototoxicity in Human Lens Epithelial Cells Promoted by St. John's Wort¶

2004 ◽  
Vol 80 (3) ◽  
pp. 583 ◽  
Author(s):  
Yu-Ying He ◽  
Colin F. Chignell ◽  
David S. Miller ◽  
Usha P. Andley ◽  
Joan E. Roberts
Keyword(s):  
Epithelial Cells ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
St John’S Wort ◽  
Human Lens Epithelial Cells ◽  
St John's Wort

scholarly journals P 229 Growth of human lens epithelial cells on the anterior lens capsule in vitro

1995 ◽  
Vol 35 ◽  
pp. S199
Author(s):  
J.H. Meyer ◽  
J. Schmidt ◽  
F. Eppinger ◽  
B. Flügel ◽  
K.U. Löffler ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Lens Capsule ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells ◽  
Anterior Lens Capsule
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