scholarly journals MicroRNA-34a promoting apoptosis of human lens epithelial cells through down-regulation of B-cell lymphoma-2 and silent information regulator

2016 ◽  
Keyword(s):  
Epithelial Cells ◽  
B Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Down Regulation ◽  
Human Lens Epithelial Cells

scholarly journals MicroRNA-15a modulates lens epithelial cells apoptosis and proliferation through targeting B-cell lymphoma-2 and E2F transcription factor 3 in age-related cataracts

2019 ◽  
Vol 39 (12) ◽  
Author(s):  
Qiao Li ◽  
HaiTao Pan ◽  
QingHuai Liu
Keyword(s):  
Cell Proliferation ◽  
Transcription Factor ◽  
B Cell ◽  
Cell Apoptosis ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Age Related ◽  
E2f Transcription Factor

Abstract Age-related cataract remains a serious problem in the aged over the world. MicroRNAs are abnormally expressed in various diseases including age-related cataract. MicroRNA-15a (MicroRNA-15a) has been involved in various diseases and plays crucial roles in many cellular processes. However, the mechanism of microRNA-15a in the genesis of cataract remains barely known. We therefore aimed to investigate the role of microRNA-15a in the cataract. Herein, human lens epithelial B3 cells, HLE-B3 cells were treated with 200 μmol/l H2O2 for 24 h. H2O2 was utilized in our study to induce HLE-B3 cells injury. We observed that cell apoptosis was induced by the treatment of H2O2 and meanwhile, cell proliferation was repressed by 200 μmol/l H2O2. Then, it was found that microRNA-15a was significantly increased with the H2O2 exposure in vitro. Importantly, B-cell lymphoma-2 (BCL2) and E2F transcription factor 3 (E2F3) exert crucial roles in cell apoptosis and cell proliferation. We found that BCL2 and E2F3 were greatly reduced by 200 μmol/l H2O2 in human lens epithelial cells. In addition, microRNA-15a overexpression induced cell apoptosis and repressed cell proliferation through suppressing BCL2 and E2F3. Subsequently, BCL2 and E2F3 were predicted as a direct target of microRNA-15a. The direct correlation between microRNA-15a and BCL2/E2F3 was confirmed by dual luciferase reporter assay. In conclusion, we demonstrated that microRNA-15a triggered apoptosis and repressed the proliferation of HLE-B3 cells by modulating BCL2 and E2F3.

Phototoxicity in Human Lens Epithelial Cells Promoted by St. John's Wort¶

2004 ◽  
Vol 80 (3) ◽  
pp. 583 ◽  
Author(s):  
Yu-Ying He ◽  
Colin F. Chignell ◽  
David S. Miller ◽  
Usha P. Andley ◽  
Joan E. Roberts
Keyword(s):  
Epithelial Cells ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
St John’S Wort ◽  
Human Lens Epithelial Cells ◽  
St John's Wort

scholarly journals P 229 Growth of human lens epithelial cells on the anterior lens capsule in vitro

1995 ◽  
Vol 35 ◽  
pp. S199
Author(s):  
J.H. Meyer ◽  
J. Schmidt ◽  
F. Eppinger ◽  
B. Flügel ◽  
K.U. Löffler ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Lens Capsule ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells ◽  
Anterior Lens Capsule

Mitochondria-Targeted Antioxidant Peptide SS31 Protects Cultured Human Lens Epithelial Cells against Oxidative Stress

2014 ◽  
Vol 40 (8) ◽  
pp. 822-829 ◽  
Author(s):  
Meng Cai ◽  
Jing Li ◽  
Shaofen Lin ◽  
Xiaoyun Chen ◽  
Juan Huang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Antioxidant Peptide ◽  
Human Lens Epithelial Cells
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