Oxidative Damage to Human Lens Epithelial Cells in Culture: Estrogen Protection of Mitochondrial Potential, ATP, and Cell Viability

2003 ◽  
Vol 44 (5) ◽  
pp. 2067 ◽  
Author(s):  
Xiaofei Wang ◽  
James W. Simpkins ◽  
James A. Dykens ◽  
Patrick R. Cammarata
Keyword(s):  
Epithelial Cells ◽  
Oxidative Damage ◽  
Cell Viability ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Mitochondrial Potential ◽  
Human Lens Epithelial Cells ◽  
Cells In Culture

scholarly journals Thioredoxin Binding Protein-2 Regulates Autophagy of Human Lens Epithelial Cells under Oxidative Stress via Inhibition of Akt Phosphorylation

2016 ◽  
Vol 2016 ◽  
pp. 1-17 ◽  
Author(s):  
Jiaojie Zhou ◽  
Ke Yao ◽  
Yidong Zhang ◽  
Guangdi Chen ◽  
Kairan Lai ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Cell Viability ◽  
Binding Protein ◽  
Negative Regulator ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells ◽  
Age Related

Oxidative stress plays an essential role in the development of age-related cataract. Thioredoxin binding protein-2 (TBP-2) is a negative regulator of thioredoxin (Trx), which deteriorates cellular antioxidant system. Our study focused on the autophagy-regulating effect of TBP-2 under oxidative stress in human lens epithelial cells (LECs). Human lens epithelial cells were used for cell culture and treatment. Lentiviral-based transfection system was used for overexpression of TBP-2. Cytotoxicity assay, western blot analysis, GFP/mCherry-fused LC3 plasmid, immunofluorescence, and transmission electronic microscopy were performed. The results showed that autophagic response of LECs with increased LC3-II, p62, and GFP/mCherry-LC3 puncta (P<0.01) was induced by oxidative stress. Overexpression of TBP-2 further strengthens this response and worsens the cell viability (P<0.01). Knockdown of TBP-2 attenuates the autophagic response and cell viability loss induced by oxidative stress. TBP-2 mainly regulates autophagy in the initiation stage, which is mTOR-independent and probably caused by the dephosphorylation of Akt under oxidative stress. These findings suggest a novel role of TBP-2 in human LECs under oxidative stress. Oxidative stress can cause cell injury and autophagy in LECs, and TBP-2 regulates this response. Hence, this study provides evidence regarding the role of TBP-2 in lens and the possible mechanism of cataract development.

Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells

2012 ◽  
Vol 18 (7) ◽  
pp. 529-533 ◽  
Author(s):  
Chun-yan Feng ◽  
Xiu-rong Huang ◽  
Ming-xin Qi ◽  
Song-wen Tang ◽  
Yan-hong Hu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Oxidative Damage ◽  
Proteomic Analysis ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells

Intracellular Effects of NSAIDs/ASA in Oxidatively Stressed Human Lens Epithelial Cells in Culture

2008 ◽  
Vol 40 (2) ◽  
pp. 77-85 ◽  
Author(s):  
A. Petersen ◽  
T. Carlsson ◽  
J.-O. Karlsson ◽  
M. Zetterberg
Keyword(s):  
Epithelial Cells ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells ◽  
Cells In Culture
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