scholarly journals Preliminary results of the monitoring bio-ecological features of plants Fritillaria ruthenica Wikstr. in the model forest habitats of the Krasnosamarsky forest

2016 ◽  
Vol 5 (2) ◽  
pp. 33-38
Author(s):  
Mariya Gennadevna Kotelnikova
Keyword(s):  
Significant Proportion ◽  
Plant Conservation ◽  
Growth Conditions ◽  
Shoot Length ◽  
Anthropogenic Factors ◽  
Good Growth ◽  
General Description ◽  
Science Literature ◽  
Preliminary Results ◽  
Ecological Features

The article presents some preliminary results of the monitoring of Fritillaria ruthenica Wikstr. plants (category of rare and endangered plant species, included in the Red Books of Russia and Samara Region). By summarizing the available sources of science literature we have compiled a general description of morphological and bio-ecological species characteristics. The results of the field study fulfilled in 2012-2014 in Krasnosamarsky forestry (Samara region) were used to determine morphometric parameters of Fritillaria ruthenica plants. The data also helped us to assess the variability level and to make a comparison with plant quantitative traits given in the literature. It was found that the shoot length of Fritillaria ruthenica plants from model biotope in Krasnosamarsky forest in 2012-2014 varied in the range of 20 ... 70 cm, in 2012 and 2013 individuals with long shoots about 50 cm dominated, in 2014 - with shoot length about 50 and 60 cm. The feature of the shoot length is characterized by plasticity, the highest level of variation was observed in 2013. The lengths of shoots are inside the range specified for the various parts of the area in the literature with a significant proportion of tall plants in the studied population. This may be a sign of good growth conditions for Fritillaria ruthenica that gives an opportunity to the plant conservation in natural communities in the absence of limited anthropogenic factors.

scholarly journals The assessment of bio-ecological features of plants Tulipa biebersteiniana Schult. et Schult. fil. in the model forest habitats of the Krasnosamarsky forest

2016 ◽  
Vol 5 (3) ◽  
pp. 30-35
Author(s):  
Mariya Gennadevna Kotelnikova
Keyword(s):  
Average Length ◽  
Significant Proportion ◽  
Plant Conservation ◽  
Population Group ◽  
Shoot Length ◽  
Anthropogenic Factors ◽  
Good Growth ◽  
General Description ◽  
Science Literature ◽  
Ecological Features

The paper presents some preliminary results of the monitoring of Tulipa biebersteiniana Schult. et Schult. fil. plants (category of rare and endangered plant species). By summarizing the available sources of science literature we have compiled a general description of morphological and bio-ecological species characteristics. The results of the field study fulfilled in 2012-2015 in two population groups of the Krasnosamarsky forestry (Samara region) were used to determine morphometric parameters of Tulipa biebersteiniana plants. The data also helped us to assess the variability level and to make a comparison with plant quantitative traits given in the literature. It was found that the shoot length of Tulipa biebersteiniana plants varied in the range of 23 50 cm, the maximum average length of 40 cm are presented in plants of population group number two. The distribution of shoot length among individuals of the second population group is more stable, changes depending on the year of vegetation are poorly expressed. The lengths of shoots inside the range specified for the various parts of the area in the literature with a significant proportion of high plants in the population is studied. This may be a sign of good growth conditions for Fritillaria ruthenica that gives an opportunity to the plant conservation in natural communities in the absence of limiting anthropogenic factors.

scholarly journals Bio-ecological features of some rare plants fruits in the Samara Region

2017 ◽  
Vol 6 (1) ◽  
pp. 41-46
Author(s):  
Mariya Gennadevna Kotelnikova
Keyword(s):  
Plant Species ◽  
Fruit Size ◽  
Significant Proportion ◽  
Rare Plants ◽  
Anthropogenic Factors ◽  
Population Groups ◽  
Fruit Length ◽  
Ecological Features ◽  
Rare And Endangered ◽  
The Difference

The paper presents some preliminary results of the monitoring of Fritillaria meleagroides Partin ex Schult. et Schult. fil. and Tulipa biebersteiniana Schult. et Schult. fil. plants (category of rare and endangered plant species). The results of the field study fulfilled in 2012-2016 in two population groups of quarter 80 and cenopopulation of quarter 69 in the Krasnosamarsky forestry (Samara Region) were used to determine morphometric parameters of plant species. The data also helped us to assess the variability level and to make a comparison with plant quantitative traits given in the literature. It has been found that the sample indicators fit into the range of values specified in literature sources, with a significant proportion of large fruit 2,0-3,0 cm in length. The dynamics of the fruit size of Fritillaria meleagroides Partin ex Schult. et Schult. fil. and Tulipa biebersteiniana Schult. et Schult. fil. plants in population groups of quarter 80, as a whole, has general patterns in the difference during some seasons of vegetation. Prevailing values of fruit length of plants in cenopopulations of quarter 69 changed slightly according to year of vegetation, these populations are more resistant and numerous comparing with the populations of quarter 80. As for variability of signs, the values of fruit length and width coefficients of variation does not exceed 25% for Fritillaria meleagroides Partin ex Schult. et Schult. fil. plants and 20% for Tulipa biebersteiniana Schult. et Schult. fil. plants which is optimal for this type of indicators and is consistent with the bioecological specificity of fruit plant. The conditions of ecosystems in 69 and 80 quarters of Krasnosamarsky forestry are favorable for the vegetation of two species plants, the successful preservation and restoration in natural communities can be in the absence of anthropogenic factors limits.

scholarly journals Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1610
Author(s):  
Branka Vinterhalter ◽  
Nevena Banjac ◽  
Dragan Vinterhalter ◽  
Dijana Krstić-Milošević
Keyword(s):  
Hairy Root ◽  
Large Scale ◽  
Bubble Column ◽  
Growth Parameters ◽  
Growth Index ◽  
Dry Weight ◽  
Growth Conditions ◽  
Good Growth ◽  
Gentiana Dinarica ◽  
Hairy Root Clones

The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.

Growth Conditions of a Thermophilic Geobacillus sp. as an Enhanced Oil Recovery Material

2012 ◽  
Vol 496 ◽  
pp. 457-460
Author(s):  
Xiang Ping Kong
Keyword(s):  
Enhanced Oil Recovery ◽  
Bacterial Growth ◽  
Oil Recovery ◽  
Nitrogen Sources ◽  
Growth Conditions ◽  
Good Growth ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

The growth conditions of a Geobacillus sp. were investigated by single-factor experiments. The strain was strictly aerobic bacterium, and could grow on hydrocarbons as the sole carbon source. The optimum carbon and nitrogen sources were 3.0% sucrose and 0.20% KNO3, respectively. The range of temperature, salinity and pH for the bacterial growth was 35-70 °C, 0-10% NaCl and 5.5-9.5, and good growth was obtained at 35-65 °C, 0.5-8% NaCl and 6.0-9.0, respectively. Particularly, the optimum temperature for the bacterial growth was between 50 °C and 60 °C. The strain had wide adaptability to the extreme conditions, and may be potentially applied to microbial enhanced oil recovery and oil-waste bioremediation technology.

scholarly journals Oceanithermus desulfurans sp. nov., a novel thermophilic, sulfur-reducing bacterium isolated from a sulfide chimney in Suiyo Seamount

2004 ◽  
Vol 54 (5) ◽  
pp. 1561-1566 ◽  
Author(s):  
Koji Mori ◽  
Takeshi Kakegawa ◽  
Yowsuke Higashi ◽  
Ko-ichi Nakamura ◽  
Akihiko Maruyama ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Growth Conditions ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
Good Growth ◽  
Chain Link ◽  
Circular Structure ◽  
Hybridization Test ◽  
A Chain ◽  
Cellular Fatty Acid Profile

A novel thermophilic, microaerophilic, sulfur-reducing bacterium designated strain St55BT was isolated from a sulfide chimney in the hydrothermal field of Suiyo Seamount (Izu-Bonin Arc, Western Pacific). Cells of the isolate were rod-shaped and tended to form a chain-link circular structure (a rotund body) at exponential phase under good growth conditions. The isolate was a chemoheterotroph requiring yeast extract for growth. Although strain St55BT used oxygen as an electron acceptor, it could not form colonies in an oxygen concentration of more than 5 % (v/v). The isolate also used nitrate, nitrite or elemental sulfur in the absence of oxygen. A phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate was closely related to Oceanithermus profundus, belonging to the phylum ‘Deinococcus–Thermus’ (sequence similarity 99·5 %). However, strain St55BT differed from O. profundus in terms of usage of electron donors, cellular fatty acid profile and DNA G+C content. In addition, a DNA–DNA hybridization test indicated low relatedness between the isolate and O. profundus. For the reasons given above, the name Oceanithermus desulfurans sp. nov. is proposed for strain St55BT (=NBRC 100063T=DSM 15757T).

scholarly journals Screening of Highly Expressed Mycobacterial Genes Identifies Rv3615c as a Useful Differential Diagnostic Antigen for the Mycobacterium tuberculosis Complex

2008 ◽  
Vol 76 (9) ◽  
pp. 3932-3939 ◽  
Author(s):  
Ben Sidders ◽  
Chris Pirson ◽  
Philip J. Hogarth ◽  
R. Glyn Hewinson ◽  
Neil G. Stoker ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Significant Proportion ◽  
Growth Conditions ◽  
Control Measures ◽  
Human Populations ◽  
Infected Cattle ◽  
Ifn Γ ◽  
Mycobacterial Antigens ◽  
And Control ◽  
Differential Antigens

ABSTRACT Tuberculous infections caused by mycobacteria, especially tuberculosis of humans and cattle, are important both clinically and economically. Human populations can be vaccinated with Mycobacterium bovis bacille Calmette-Guérin (BCG), and control measures for cattle involving vaccination are now being actively considered. However, diagnostic tests based on tuberculin cannot distinguish between genuine infection and vaccination with BCG. Therefore, identification of differential diagnostic antigens capable of making this distinction is required, and until now sequence-based approaches have been predominant. Here we explored the link between antigenicity and mRNA expression level, as well as the possibility that we may be able to detect differential antigens by analyzing quantified global transcriptional profiles. We generated a list of 14 candidate antigens that are highly expressed in Mycobacterium tuberculosis and M. bovis under a variety of growth conditions. These candidates were screened in M. bovis-infected and naïve cattle for the ability to stimulate a gamma interferon (IFN-γ) response. We identified one antigen, Rv3615c, which stimulated IFN-γ responses in a significant proportion of M. bovis-infected cattle (11 of 30 cattle [37%] [P < 0.01]) but not in naïve or BCG-vaccinated animals. Importantly, the same antigen stimulated IFN-γ responses in a significant proportion of infected cattle that did not respond to the well-characterized mycobacterial antigens ESAT-6 and CFP-10. Therefore, use of the Rv3615c epitope in combination with previously described differential tests based on ESAT-6 and CFP-10 has the potential to significantly increase diagnostic sensitivity without reducing specificity in BCG-vaccinated populations.

Cell heterogeneity upon myogenic differentiation: down-regulation of MyoD and Myf-5 generates ‘reserve cells’

1998 ◽  
Vol 111 (6) ◽  
pp. 769-779 ◽  
Author(s):  
N. Yoshida ◽  
S. Yoshida ◽  
K. Koishi ◽  
K. Masuda ◽  
Y. Nabeshima
Keyword(s):  
Cell Cycle ◽  
Myogenic Differentiation ◽  
Significant Proportion ◽  
Ectopic Expression ◽  
Terminal Differentiation ◽  
Growth Conditions ◽  
Down Regulation ◽  
Undifferentiated Cells ◽  
Myoblast Cells ◽  
Myod Expression

When a proliferating myoblast culture is induced to differentiate by deprivation of serum in the medium, a significant proportion of cells escape from terminal differentiation, while the rest of the cells differentiate. Using C2C12 mouse myoblast cells, this heterogeneity observed upon differentiation was investigated with an emphasis on the myogenic regulatory factors. The differentiating part of the cell population followed a series of well-described events, including expression of myogenin, p21(WAF1), and contractile proteins, permanent withdrawal from the cell cycle and cell fusion, whereas the rest of the cells did not initiate any of these events. Interestingly, the latter cells showed an undetectable or greatly reduced level of MyoD and Myf-5 expression, which had been originally expressed in the undifferentiated proliferating myoblasts. When these undifferentiated cells were isolated and returned to the growth conditions, they progressed through the cell cycle and regained MyoD expression. These cells demonstrated identical features with the original culture on the deprivation of serum. They produced both MyoD-positive differentiating and MyoD-negative undifferentiated populations once again. Thus the undifferentiated cells in the serum-deprived culture were designated ‘reserve cells’. Upon serum deprivation, MyoD expression rapidly decreased as a result of down-regulation in approximately 50% of the cells. After this heterogenization, MyoD positive cells expressed myogenin, which is the earliest known event of terminal differentiation and marks irreversible commitment to this, while MyoD-negative cells did not differentiate and became the reserve cells. We also demonstrated that ectopic expression of MyoD converted the reserve cells to differentiating cells, indicating that down-regulation of MyoD is a causal event in the formation of reserve cells.

scholarly journals Bio-ecological features of Salsola Richteri Kar. in cultural conditions

2020 ◽  
Vol 1 ◽  
pp. 50-52
Author(s):  
Baltabaev Muratbay ◽  
Kalbaeva Sarigul
Keyword(s):  
Root System ◽  
Good Growth ◽  
Seed Productivity ◽  
Cultural Conditions ◽  
Ecological Features

The article is devoted to the study of the bio-ecological features of the Richter's saltwort (salsola richteri kar.) In cultural conditions. Good growth of Cherkez on bare sands, high seed productivity, the ability to multiply by seeds and cuttings and tolerate significant salinity, powerful growth of the root system contributed to its promotion as a promising plant when the sands were consolidated.

STRUCTURE, GROWTH PROCESS OF TREE STUDS OF LIQUE LEAF IN THE CONDITIONS OF BURZYAN FORESTRY

2020 ◽  
Vol 37 (3) ◽  
pp. 91-99
Author(s):  
A.Yu. Khismatullin ◽  
Keyword(s):  
Growth Process ◽  
Average Diameter ◽  
Growth Conditions ◽  
Arithmetic Mean ◽  
Forest Area ◽  
Good Growth ◽  
Model Trees ◽  
Design Object ◽  
Different Ages ◽  
The Difference

The article presents the results of assessing the structure and growth of smallleaved Linden stands in the conditions of the burzyansk forest area. Regularities of small-leaved Linden renewal in the studied territory are revealed. The distribution curves of Linden trees by diameter have positive asymmetry and kurtosis. The difference in the arithmetic mean, mode, and median values, as well as the true average diameter, indicate that the stand is of different ages. Analysis of the growth of model trees indicates good growth conditions in the conditions of the design object, but in recent years there has been a decrease in growth in all indicators, which indicates the need for thinning of the stand.

FLABRA, frontline approach for BRCA testing in ovarian cancer (OC) treatment naïve population: A Latin America (LA) epidemiologic study.

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. e17050-e17050
Author(s):  
Susana Beatriz Goncalves ◽  
Gonzalo Giornelli ◽  
Marcelo Horacio Pereira ◽  
Dolores Gallardo-Rincon ◽  
Maria Del Pilar Estevez-Diz
Keyword(s):  
Genetic Counseling ◽  
Latin American ◽  
Somatic Mutations ◽  
Significant Proportion ◽  
Epidemiologic Study ◽  
Parp Inhibitors ◽  
Brca Testing ◽  
Brca Mutations ◽  
Cross Sectional ◽  
Preliminary Results

e17050 Background: The majority of OC cases are sporadic, but it is estimated that in 17%, germline mutations in BRCA1 or BRCA2 genes can be identified. BRCA mutated OC has distinct clinical characteristics, increased sensitivity to platinum and non-platinum agents, and to DNA damage repair (DDR) targeting agents like PARP inhibitors. Additionally, somatic BRCA mutations could be identified in tumor tissue. The prevalence of germline BRCA mutations (gBRCAm) and somatic mutations (sBRCAm) has been not been characterized in Latin-american population, which is a paradigm of poly-ethnicity, where prevalence of germline, but especially somatic BRCA mutations in OC, has not been studied. Furthermore, tumor testing as first step may be a new option in BRCA testing algorithm that could avoid the necessity for double testing (gBRCA, then sBRCA testing), in case of gBRCAm negative result. Methods: FLABRA is a cross-sectional, multi-center, study designed to determine the prevalence of sBRCAm in newly diagnosed OC patients versus gBRCAm, and to describe different treatment approaches at front line in LA, as well as current OC genetic counselling. We enrolled 400 consecutive patients from 40 institutions in Argentina, Brazil, Colombia, Mexico, Peru and Panama, diagnosed with OC. Tumor blocks were tested for sBRCA (Myriad Tumor BRACAnalysis CDx™). In sBRCA positive patients, blood samples were analyzed to confirm if the mutation was germline or somatic in origin. In gBRCAm, genetic counseling was advised. Medical records were reviewed for data relevant to medical history, surgery results, treatment approach and genetic counseling. Results: We present the preliminary results with 291 patients already tested. For this first subset of patients, 85/291 (29%) had BRCA mutations identified in their tumors. Preliminary results confirm that starting with tumor testing enlarges the population eligible for PARP inhibitors, by identifying additional patients with somatic mutations only detectable in the tumor. Although preliminary, our data confirms the possibility of identifying additional patients with sBRCA mutations by testing in tumor, with a more cost-effective approach, avoiding a second round of gBRCA testing in patients with BRCA negative results in tumor. Conclusions: In this preliminary analysis we found that somatic BRCA mutations account for a significant proportion from total BRCA mutations within LA population studied.

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