gentiana dinarica
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Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1610
Author(s):  
Branka Vinterhalter ◽  
Nevena Banjac ◽  
Dragan Vinterhalter ◽  
Dijana Krstić-Milošević

The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.


2017 ◽  
Vol 130 (3) ◽  
pp. 631-640 ◽  
Author(s):  
Dijana Krstić-Milošević ◽  
Teodora Janković ◽  
Branka Uzelac ◽  
Dragan Vinterhalter ◽  
Branka Vinterhalter

2015 ◽  
Vol 121 (3) ◽  
pp. 667-679 ◽  
Author(s):  
Branka Vinterhalter ◽  
Dijana Krstić-Milošević ◽  
Teodora Janković ◽  
Dejan Pljevljakušić ◽  
Slavica Ninković ◽  
...  

2013 ◽  
Vol 71 (2) ◽  
pp. 147-155 ◽  
Author(s):  
Dijana Krstić-Milošević ◽  
Teodora Janković ◽  
Branka Vinterhalter ◽  
Nebojša Menković ◽  
Ivana Aljančić ◽  
...  

2012 ◽  
Vol 35 (2) ◽  
pp. 567-574 ◽  
Author(s):  
Vinterhalter Branka ◽  
Krstić-Milošević Dijana ◽  
Janković Teodora ◽  
Zdravković-Korać Snežana ◽  
Vinterhalter Dragan

2012 ◽  
Vol 7 (4) ◽  
pp. 690-697 ◽  
Author(s):  
Branka Vinterhalter ◽  
Dijana Milošević ◽  
Teodora Janković ◽  
Jelena Milojević ◽  
Dragan Vinterhalter

AbstractGentiana dinarica Beck, rare and endangered species of Balkan Dinaric alps, was in vitro propagated (micropropagated) from axillary buds of plants collected at Mt. Tara, Serbia. G. dinarica preferred MS to WPM medium, with optimal shoot multiplication on MS medium with 3% sucrose, 1.0 mg l−1 BA and 0.1 mg l−1 NAA. Rooting was not clearly separated from shoot multiplication since BA did not completely inhibit root initiation. Spontaneous rooting on plant growth regulator-free medium occurred in some 30% of shoot explants. Rooting was stimulated mostly by decreased mineral salt nutrition and a medium with 0.5 MS salts, 2% sucrose and 0.5–1.0 mg l−1 IBA was considered to be optimal for rooting. Rooted plantlets were successfully acclimated and further cultured in peat-based substrate.


2004 ◽  
Vol 32 (10) ◽  
pp. 937-941 ◽  
Author(s):  
Dijana Krstić ◽  
Teodora Janković ◽  
Ivana Aljančić ◽  
Katarina Šavikin-Fodulović ◽  
Nebojša Menković ◽  
...  

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