scholarly journals Features of inflammation course in rats with experimental hyper- and hypothyroidism

2013 ◽  
Vol 94 (5) ◽  
pp. 726-730 ◽  
Author(s):  
N N Mayanskaya ◽  
S S Rimar ◽  
S D Mayanskaya
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Wistar Rats ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
Inflammatory Reactions ◽  
Experimental Hyperthyroidism ◽  
Functional Reserves ◽  
Experimental Inflammation ◽  
Experimental Thyroid

Aim. To mark out the features of inflammatory reactions alterations in rats with experimental thyroid gland dysfunction. Methods. Experimental hypothyroidism was modeled by daily thiamazole intake in 50 Wistar rats. Hyperthyroidism was modeled with daily lyothyronine intake in 50 Wistar rats. An inflammation was modeled by artificial gum injury. 10 intact rats were examined as a control group. Results. Experimental hypothyroidism was accompanied by leukocyte biocidity decrease both in blood and periodontium, decreased lysosomal enzymes activity and antioxidant activity, and accumulation of lipid peroxidation products. Periodontal suppurations at experimental inflammation were 1.5 more frequent in rats with hypothyroidism compared to controls due to decreased leukocyte biocidity and antioxidant activity. Experimental hyperthyroidism was associated with increased biocidity and decreased functional reserves in phagocytes both in blood and periodontium, as well as antioxidant activity decrease and lipid peroxidation intensification. Changes of tumor necrosis factor alpha blood concentration both in hypothyroidism and hyperthyroidism corresponded with changes of phagocyte biocidity. The rate of periodontal suppuration in rats with hyperthyroidism was twice lower on the day 7 compared to controls due to high leukocyte and macrophage biocidity. Conclusion. Experimental hypothyroidism was accompanied by intensifying of lipid peroxidation and decreased tissue reparation ability; experimental hyperthyroidism was associated with increased biocidity and decreased functional reserves in phagocytes, and less evident decrease of compensatory abilities of antioxidant system lipid peroxidation intensification compared to hypothyroidism.

scholarly journals The effect of bee venom and hyperthermia on the lipoperoxidation and antioxidant activity of the blood of tumor-bearing animals

2021 ◽  
pp. 25-33
Author(s):  
M.A. Shabalin ◽  
◽  
A.V. Deryugina ◽  
V.V. Nazarova ◽  
E.A. Gracheva ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Malignant Tumors ◽  
Combined Treatment ◽  
Bee Venom ◽  
Control Group ◽  
Sod Activity ◽  
Tumor Bearing ◽  
Prolonged Effect ◽  
Control Tumor

Introduction. Nowadays, it has been shown that one of the possible ways to increase the effectiveness of the treatment of malignant tumors is the use of combined treatment methods. Aim. To study the intensity of lipid peroxidation (LPO) and the activity of superoxide dismutase (SOD) in hyperthermia against the background of bee venom in the blood of tumor-bearing rats. Materials and methods. The experimental animals were divided into 5 groups: 1st — intact; 2nd — control (tumor-bearing animals (PS-1 tumor strain) with intraperitoneal (IP) administration of saline solution); 3rd, 4th and 5th groups — tumor-bearing animals with IP administration of 0.5 ml of bee venom against the background of hyperthermia 42, 43 and 44°C respectively. The content of diene and triene conjugates, Schiff bases (SB) and SOD activity in the blood of animals was determined. Results. From the 1st day after the end of the experiment, a statistically significant decrease in triene conjugates was recorded in all experimental groups compared to the control group. SB decreased on the 1st–7th day after the end of the experiment with the action of hyperthermia 42°C and bee venom, on the 7th–28th day — with the action of hyperthermia 43°C and bee venom, and on the 28th day — with the action of bee venom and hyperthermia 44°C, which was accompanied by an increase in SOD activity from the 7th day in all experimental groups compared to the control group. Conclusion. Hyperthermia in combination with the action of bee venom causes a decrease in lipid peroxidation products and an increase in antioxidant activity in the blood of tumor-bearing rats. The most effective action, in our opinion, is the use of bee venom against the background of hyperthermia of 43°C, at which a prolonged effect is recorded both with respect to a decrease in the concentration of SB and an increase in the activity of SOD.

scholarly journals Pharmacological and Genotoxic Properties of Polyphenolic Extracts ofCedrela odorataL. andJuglans regiaL. Barks in Rodents

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Dulce Carolina Almonte-Flores ◽  
Norma Paniagua-Castro ◽  
Gerardo Escalona-Cardoso ◽  
Martha Rosales-Castro
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Comet Assay ◽  
Juglans Regia ◽  
Control Group ◽  
Cedrela Odorata ◽  
Hepatic Lipid ◽  
Polyphenolic Extract ◽  
Hepatic Lipid Peroxidation

Evaluation of the phenolic compounds and antioxidant activity ofCedrela odorataL. andJuglans regiaL. bark extracts was performedin vitro.Juglans regiashowed greater extract concentration and higher antioxidant activity. Hypoglycemic activity in rats was assessed by generating a glucose tolerance curve and determining the area under the curve (AUC). Diabetes was later induced by an injection with streptozotocin (65 mg/kg of b.w.) and confirmed after 24 hours. The extract was administered (200 mg/kg b.w.) over 10 days, and blood glucose was monitored and compared with a control group. The glucose AUC showed a hypoglycemic effect ofJ. regiaandC. odoratain normal rats. Both extracts reduced hepatic lipid peroxidation in diabetic rats. Polyphenolic extracts reduced cholesterol levels in a hypercholesterolemic mouse model and decreased hepatic lipid peroxidation. Polyphenolic extract doses of 100 and 200 mg/kg b.w. were administered alone or with cyclophosphamide (CPA) 50 mg/kg ip, which was used as a positive control. Analyses were performed using leukocytes in a comet assay after 4 and 24 h of treatment. Genotoxic effects were evaluated by the comet assay, which showed that whileJ. regiaextract had no effect,C. odorataextract induced slight damage at 200 mg/kg, with the formation of type 0 and 1 comets.

scholarly journals Features of cellular and molecular inflammatory mechanisms involvement in hormone-mediated myocardial experimental injury

2013 ◽  
Vol 94 (5) ◽  
pp. 723-726
Author(s):  
N N Mayanskaya ◽  
L D Hidirova ◽  
S D Mayanskaya
Keyword(s):  
Myocardial Infarction ◽  
Lipid Peroxidation ◽  
Tumor Necrosis ◽  
Wistar Rats ◽  
Biocidal Activity ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Necrosis Factor ◽  
Tetrazolium Test ◽  
Pro Inflammatory Cytokines

Aim. To study the features of inflammatory effector mechanisms variation in Wistar rats with metabolic non-coronary myocardial infarction. Methods. Metabolic myocardial infarction was reproduced in Wistar rats by adrenalin injection. Metabolic myocardial infarction was verified by electrocardiography and histological examination. Biocidal activity of blood neutrophils was determined by nitro blue tetrazolium test and chemiluminescence, cytokine serum levels (interleukin-1β, interleukin 6 and tumor necrosis factor alpha) were determined by ELISA. Lipid peroxidation was assessed by measuring malondialdehyde, diene conjugates and diketones blood concentrations. Catalase activity and reduced glutathione level were determined in erythrocyte hemolysate, serum activity of superoxide dismutase was also measured. Intact animals were examined as a control group. Results. In rats with metabolic myocardial infarction, oxygen-dependent leukocyte biocidity (determined by nitro blue tetrazolium test and chemiluminescence) increased dramatically from the first day of the adrenalin administration and continued to increase until the end of the experiment (day 14). Accordingly, the production of active oxygen metabolites, which intensified the lipid peroxidation, was increasing. Simultaneously an imbalance between pro-and antioxidant system parameters was detected. Serum concentration of pro-inflammatory cytokines (tumor necrosis factor alpha, interleukin-1β, interleukin-6) increased. Conclusion. Long-term administration of adrenalin to experimental animals causes an increase in the neutrophils biocidal activity, accompanied by release of reactive oxygen species, pro-inflammatory cytokines, lipid peroxidation intensifying and decreased compensation by antioxidant defense system, which together can be a powerful trigger of myocardial damage.

scholarly journals Antioxidants from Annatto Seeds as Possible Inhibitory Agents of the Hepatotoxicity Induced by the Antitumor Agent Cisplatin

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Lucéia Fátima Souza ◽  
Niara da Silva Medeiros ◽  
Paula Cilene Pereira dos Santos ◽  
Carlos Henrique Pagno ◽  
Cleice Dalla Nora ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Wistar Rats ◽  
Antitumor Agent ◽  
Control Group ◽  
Efficiency Coefficient ◽  
Fat Deposits ◽  
Commercial Feed ◽  
Male Wistar Rats ◽  
Pre Treatment ◽  
Inhibitory Agents

The effects of annatto seeds and of bixin on the oxidative damage induced by cisplatin in male Wistar rats was evaluated in the present study by way of lipid peroxidation, weight gain, the food efficiency coefficient, fat deposits in the hepatocytes and dosing of the enzymes in this organ. The animals were divided into four groups: control group (CG), cisplatin group (CPG), bixin+cisplatin group (CBG) and annatto+cisplatin group (CUG). Cisplatin (5 mg/kg body weight) was injected intraperitoneally 48 hours before the end of the experiment. The bixin and annatto were administered daily together with the commercial feed. The pre-treatment with annatto and bixin attenuated the cisplatin-induced liver damage and significantly reduced the enzymes AST and ALT. Annatto was shown to be capable of inhibiting lipid peroxidation as determined by TBARS. These results suggest that annatto seeds and bixin could be important agents in the reduction of cisplatin-induced hepatotoxicity.

Vitamin E as an inhibitor of oxidative damage to goose meat storage

2019 ◽  
pp. 137-144
Author(s):  
E. Danchenko ◽  
A. Ruban ◽  
L. Zdorovtseva ◽  
N. Danchenko ◽  
T. Gaponenko ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Vitamin E ◽  
Control Level ◽  
Control Sample ◽  
Test Sample ◽  
Control Group ◽  
Standard Diet ◽  
Lipid Peroxidation Product ◽  
Β Carotene

The vitamin E effect on the lipid peroxidation product content and the endogenous antioxidant activity (at -18 C during the different types of storage) has been studied in goose meat. The goose meat of three samples has been used for storage. Meat of control sample has been obtained from geese fed by the standard diet. The 1st meat test sample of geese differs from the control group by two times higher content of vitamin E (40 mg / kg) in their diet from the 42nd to the 63rd day. Meat of the 2nd test sample obtained from the control group of geese is processed by a vitamin E solution (calculated at 100 mcg per g of meat) immediately before storage. The shelf life of meat is 210 days. It has been established that the intensive accumulation of the secondary lipid peroxidation products begins from the 90th day in the goose meat during its storage. In the goose diet a double increase of the vitamin E has contributed a significant (by 27.6%, p ≤ 0.05) TBA-AP decrease in the first meat test sample in comparison with the control group at the end of the experiment. The addition of vitamin E to the diet of geese has contributed the stabilization of the antioxidant pool in their meat. It has been confirmed by a 1.88-fold lower level of TBA-AP upon initiation of peroxide oxidation of Fe2+ and by the higher coefficient of antioxidant activity (36.0%, p ≤ 0.05) in this sample in comparison with the control on the 210th day. At the end of the experiment, the vitamin E content is higher 41.7 % (p ≤ 0.01) in the first sample than in the control, β-carotene - 15.0 % (p ≤ 0.05), and vitamin A is at the level of the control sample. Processed goose meat with a solution of vitamin E also provides reliable inhibition of peroxidation processes during the first half of the experiment. However, at the end of the experiment the content of TBA-AP reaches the level of the corresponding control indices in the 2nd test sample of meat. From the 120th day, there has been the more intensive use of endogenous antioxidants. The antioxidant activity coefficient decreasing to the control level on the 210th day in this meat sample is its conformation. The meat of this sample differs by a higher content of β-carotene from the control sample significantly (by 13.5%, p ≤ 0.05). Thus, to obtain a prolonged antioxidant effect during low-temperature storage of meat it is more advisable to add vitamin E to the diet of geese in the pre-slaughter period. Key words: geese, meat storage, lipoperoxidation products, antioxidant activity, vitamins E, A, β-carotene.

Gastro-protective effects of camel milk on indomethacin-induced peptic ulcer in Wistar rats

2018 ◽  
Vol 7 (2) ◽  
pp. 42-56
Author(s):  
Mansouria Belhocine ◽  
Abdelkader Homrani ◽  
Fatima Azzouz Azzouz ◽  
Chahrazed Sakmeche
Keyword(s):  
Antioxidant Activity ◽  
Gastric Ulcer ◽  
Blood Cells ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Negative Control ◽  
Camel Milk ◽  
Control Group ◽  
Gastric Mucus ◽  
Ulcer Index

Gastric ulcer associated with the use of nonsteroidal anti-inflammatory drugs (NSAIDs) and low-dose aspirin is a major public health problem. The present study was undertaken to determine the camel milk antioxidant activity, tested on DPPH, and its gastro-protective effect, investigated in Wistar rats sub-jected to gastric ulcer induced by a nonsteroidal anti-inflammatory agent, indomethacin. The study was performed on 20 adult male Wistar rats divided into 4 groups of 5 rats each. The negative control group received distilled water, the positive control group received indomethacin, the standard group received ranitidine and the fourth group was pretreated with raw camel milk, for 15 days respectively. On the 16th day, the indomethacin was ad-ministered to all rats except those of the negative control group. The ulcerogenic effect of indomethacin was highly significant, evidenced by a large number of ulcer lesions, a remarkably high ulcer index, and an important decrease in adherent gastric mucus. Camel milk resulted in significant gastro-protection compared to indomethacin ulcerated rats as manifested by significant decrease in ulcerative lesions number, and the ulcer index with a restored gastric mucus wall. The camel milk protection percentage is close to that of ranitidine. Additionally, in indomethacin-injured rats an increase in white blood cells, granulocytes, serum transaminases, and hemoglobin levels with a lowering in red blood cells were reported. These physiological disturbances were recovered by camel milk. Camel milk seemed to have gastro-protective effect, probably through its strong antioxidant activity, and may be recommended to patients with arthritis.

scholarly journals Polyphenols from Passiflora ligularis Regulate Inflammatory Markers and Weight Gain

2021 ◽  
Vol 12 (1) ◽  
pp. 36-45
Author(s):  
Jaime Angel-Isaza ◽  
Juan Carlos Carmona-Hernandez ◽  
William Narváez-Solarte ◽  
Clara Helena Gonzalez-Correa
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Wistar Rats ◽  
Adult Population ◽  
Control Group ◽  
Chow Diet ◽  
Low Grade ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Low Grade Inflammation

Abstract Weight-related disorders affect more than half of the adult population worldwide; they are also concomitant with a state of chronic low-grade inflammation manifesting in abnormal cytokine production. The present study evaluated the effect of polyphenol and flavonoid extract from Passiflora ligularis (granadilla) on low-grade inflammation and body weight in overweight Wistar rats. To induce weight-gain, rats were fed a chow diet with 30% sucrose water and supplemented with 2.0, 2.5, and 3.0 g/L polyphenol extracts (n = 16). The design was a 3 +1 factorial model performed for 42 days (granadilla polyphenols, 3 levels of supplementation, and 1 control group). In addition to total polyphenol and total flavonoid content, the major identified and quantified polyphenol, via UHPLC, was ferulic acid. Interleukin 6 (IL-6), and cytokine tumor necrosis factor-alpha (TNF-α) were evaluated in serum. A decline in the concentration of TNF-α and in weight-gain was found in P. ligularis (granadilla) groups treated with the 2.5 g/L dose. Consumption of polyphenol extracts from granadilla inhibits interleukin-activity as an indicator of inflammation and aids in body-weight control, considering similar food intake, in overweight Wistar rats.

scholarly journals Total Phenolic Content and Antioxidant Activity of Different Types of Chocolate, Milk, Semisweet, Dark, and Soy, in Cerebral Cortex, Hippocampus, and Cerebellum of Wistar Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Niara da Silva Medeiros ◽  
Roberta Koslowsky Marder ◽  
Mariane Farias Wohlenberg ◽  
Cláudia Funchal ◽  
Caroline Dani
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Cerebral Cortex ◽  
Protein Oxidation ◽  
Total Phenolic Content ◽  
Wistar Rats ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Different Types

Chocolate is a product consumed worldwide and it stands out for presenting an important amount of phenolic compounds. In this study, the total phenolic content and antioxidant activity in the cerebral cortex, hippocampus, and cerebellum of male Wistar rats when consuming different types of chocolate, including milk, semisweet, dark, and soy, was evaluated. The total polyphenols concentration and antioxidant activity in vitro by the method of DPPH radical-scavenging test were evaluated in chocolate samples. Lipid peroxidation (TBARS), protein oxidation (carbonyl), sulfhydryl groups, and activity of SOD enzyme in cerebral cortex, hippocampus, and cerebellum of rats treated or not with hydrogen peroxide and/or chocolate were also evaluated. The dark chocolate demonstrated higher phenolic content and antioxidant activity, followed by semisweet, soy, and milk chocolates. The addition of chocolate in the diet of the rats reduced lipid peroxidation and protein oxidation caused by hydrogen peroxide. In the sulfhydryl assay, we observed that the levels of nonenzymatic defenses only increased with the chocolate treatments The SOD enzyme activity was modulated in the tissues treated with the chocolates. We observed in the samples of chocolate a significant polyphenol content and an important antioxidant activity; however, additional studies with different chocolates and other tissues are necessary to further such findings.

scholarly journals In vivo antioxidant activity of Ethanolic extract from root of Smilax zeylanica on Aluminium Chloride Induced oxidative stress in Wistar rats

2018 ◽  
Vol 8 (6-s) ◽  
pp. 48-52
Author(s):  
B. Sabari Senthil ◽  
V.K. Kalaichelvan ◽  
A. Kottai Muthu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Body Weight ◽  
Wistar Rats ◽  
Reduced Glutathione ◽  
Ethanolic Extract ◽  
Aluminium Chloride ◽  
Control Group

Objective: The objective of the present study was to investigate the Evaluation of In vivo antioxidant activity of Ethanolic extract of root of Smilax zeylanica(EESZ) on Aluminium Chloride Induced apoptosis suppressing oxidative stress  in Wistar rats. Materials and Methods: The ethanolic extract from the roots of S. china by hot continuous percolation method. The rats were divided into 5 groups and each group consists of 6 animals. Rats were treated with EESC for 150 and 300 mg/ kg of body weight and piracetam, 0.5 mg/ kg of body weight for 14 successive days after inducing oxidative stress  with aluminium chloride (100 mg/ kg of body weight) for 60 days. The lipid peroxidation level (TBARS) and antioxidant activities like Superoxide dismutase (SOD), Catalase (CAT) and reduced Glutathione (GSH) were estimated in rats. Results: AlCl3 induced rats showed increased the TBARS and decreased the antioxidant enzymes like Superoxide dismutase (SOD), Catalase (CAT) and reduced Glutathione (GSH) when compared with the control group. The EESZ at higher dose 300 mg/ kg of body weight animals were significantly (P < 0.001) reduced the TBARS and increased the anti oxidant enzymes Superoxide dismutase (SOD), Catalase (CAT) and reduced Glutathione (GSH) when compared with the AlCl3 treated group Conclusion: Findings of the present study revealed that Ethanolic extract from roots of Smilax zeylanica  may be used as a significant source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses.                    Keywords: S. zeylanica, antioxidant, ethanolic extract, TBARS, rats.

scholarly journals Identification of bioactive compounds in ethylacetate fraction of Tapinanthus bangwensis leaves that ameliorate CCl4-induced hepatotoxicity in Wistar rats

2020 ◽  
Vol 4 ◽  
pp. 239784732093150
Author(s):  
Godwin Okwudiri Ihegboro ◽  
Adamu Jibrin Alhassan ◽  
Chimaobi James Ononamadu ◽  
Mohamed Sani Sule
Keyword(s):  
Antioxidant Activity ◽  
Wistar Rats ◽  
Chemical Compounds ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Protein Levels ◽  
Ms Analysis ◽  
Normal Hepatocyte ◽  
Hepatotoxic Effect

The pharmacological effects of medicinal plants are due to the presence of certain chemical compounds present in them. Previous studies have shown that crude extracts of Tapinanthus bangwensis ( T. bangwensis) possess hepatocurative potential. However, the present study aims on evaluating the antioxidant activity as well as identifying the chemical compounds in ethylacetate fraction of T. bangwensis that ameliorate carbon tetrachloride-induced hepatotoxicity in Wistar rats. Six subfractions of ethylacetate fraction were obtained by column chromatography. The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazine (DPPH), biochemical assay determined by spectrophotometry, and compound elucidation by liquid chromatography–mass spectroscopy (LC-MS) analysis. The DPPH result shows that subfraction F0 exhibited the strongest antioxidant activity followed by F4 while F1 exhibited the lowest. Oral administration of 100 mg/kg body weight of the subfractions of ethylacetate fraction of T. bangwensis increases superoxide dismutase and catalase activities and also glutathione level and decreases malondialdehyde level compared to the positive control group. Subsequently, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, and conjugated bilirubin were reduced while albumin and total protein levels increased compared to the positive control group. However, there were no significant differences between the positive control group and the group induced and treated with the subfractions at p < 0.05. The histopathology study shows normal hepatocyte distribution with no fat deposit in the induced and treated groups while fatty liver was observed in the positive group. The anti-hepatotoxic effect was higher in F4 than in other subfraction treated groups. Hence, the LC-MS analysis of F4 reveals the presence of 8-hydroxyluteolin-8-glucoside, Avicularin, Fisetin-7-glucoside, Isoscutellarein-7-xyloside, and Isovitexin, respectively, and has been reported to exhibit antioxidant and hepatocurative activities. It can be concluded that the hepatocurative effect could be due to the chemical compounds identified above.

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