scholarly journals Features of cellular and molecular inflammatory mechanisms involvement in hormone-mediated myocardial experimental injury

2013 ◽  
Vol 94 (5) ◽  
pp. 723-726
Author(s):  
N N Mayanskaya ◽  
L D Hidirova ◽  
S D Mayanskaya
Keyword(s):  
Myocardial Infarction ◽  
Lipid Peroxidation ◽  
Tumor Necrosis ◽  
Wistar Rats ◽  
Biocidal Activity ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Necrosis Factor ◽  
Tetrazolium Test ◽  
Pro Inflammatory Cytokines

Aim. To study the features of inflammatory effector mechanisms variation in Wistar rats with metabolic non-coronary myocardial infarction. Methods. Metabolic myocardial infarction was reproduced in Wistar rats by adrenalin injection. Metabolic myocardial infarction was verified by electrocardiography and histological examination. Biocidal activity of blood neutrophils was determined by nitro blue tetrazolium test and chemiluminescence, cytokine serum levels (interleukin-1β, interleukin 6 and tumor necrosis factor alpha) were determined by ELISA. Lipid peroxidation was assessed by measuring malondialdehyde, diene conjugates and diketones blood concentrations. Catalase activity and reduced glutathione level were determined in erythrocyte hemolysate, serum activity of superoxide dismutase was also measured. Intact animals were examined as a control group. Results. In rats with metabolic myocardial infarction, oxygen-dependent leukocyte biocidity (determined by nitro blue tetrazolium test and chemiluminescence) increased dramatically from the first day of the adrenalin administration and continued to increase until the end of the experiment (day 14). Accordingly, the production of active oxygen metabolites, which intensified the lipid peroxidation, was increasing. Simultaneously an imbalance between pro-and antioxidant system parameters was detected. Serum concentration of pro-inflammatory cytokines (tumor necrosis factor alpha, interleukin-1β, interleukin-6) increased. Conclusion. Long-term administration of adrenalin to experimental animals causes an increase in the neutrophils biocidal activity, accompanied by release of reactive oxygen species, pro-inflammatory cytokines, lipid peroxidation intensifying and decreased compensation by antioxidant defense system, which together can be a powerful trigger of myocardial damage.

scholarly journals Changes in the Levels of Pro-Inflammatory Cytokines in Patients with Generalized Periodontitis and Hypertension, Depending on the Method of Treatment

2017 ◽  
Vol 24 (4) ◽  
Author(s):  
T. Vicharenko ◽  
M. Rozhko
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Interleukin 6 ◽  
Tumor Necrosis ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Factor Alpha ◽  
Necrosis Factor ◽  
Pro Inflammatory Cytokines

Inflammatory mediators have an important role in the pathogenesis of periodontal disease. One of the leading mediators of the initiation of the pathological process is interleukin-1 (IL-1) – an endogenous pyrogen, a lymphocyte-activating factor. Numerous pro-inflammatory effects of interleukin-1β (IL-1β) occur in synergy with tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6), effects on hematopoiesis, participates in nonspecific anti-infective defense.The objective of the study is to determine levels of interleukin-6 and tumor necrosis factor alpha (TNF-α) in patients with hypertension II stage and generalized periodontitis of the II degree depending on the treatment method.There were examined 30 patients with hypertension of the II stage and with generalized periodontitis of the II degree. Patients’ age ranged from 35 to 54 years. These patients were divided into two groups. The control group included 10 patients without general somatic pathology and with healthy periodontitis of the same age. The result of the analysis of tumor necrosis factor alpha (TNF-α) in patients in the first group before the treatment was 10.69±2.33 pg/ml. After the treatment this indicator was 6.97±1.57 pg/ml (p>0.1) in patients of the first group.In patients of the second group the tumor necrosis factor alpha (TNF-α) was 9.49±2.2 pg/ml; after the treatment according to the offered scheme this figure decreased up to 2.77±0.9 pg/ml (p<0.01). The level of tumor necrosis factor alpha (TNF-α) in the control group was 1.5±0.77 pg/ml.Interleukin-6 was 9.91±2.04 pg/ml before the treatment in the first group. After the treatment according to the standard scheme, the level of interleukin-6 was 6.33±0.97 pg/ml (p>0.1). In the second group, before the treatment the level of  interleukin-6 was 9.65±2.41 pg/ml; after the treatment according to the offered scheme it was 2.62±0.5 pg/ml (p<0.01). In the control group the interleukin-6 level was 2.24±0.51 pg/ml.Analyzing the obtained results after the treatment in both groups we can conclude: after the treatment of generalized periodontitis of the II degree in patients with hypertension of the II stage, indices of pro-inflammatory cytokines decreased and ranged in normal limits; in patients from the second group (who received the offered scheme of treatment -including medicines) indexes of pro-inflammatory cytokines were significantly lower than in patients with the standard treatment scheme; the proposed scheme of treatment is more effective for treatment patients with generalized periodontitis of the II degree and hypertension of the II stage.

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