Microfluidic device platform operation for processing tissue into single cells v2 (protocols.io.bt9ynr7w)

protocols.io ◽  
2021 ◽  
Author(s):  
Jeremy Lombardo
Keyword(s):  
Microfluidic Device ◽  
Single Cells

A microfluidic device enabling deterministic single cell trapping and release

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Huichao Chai ◽  
Yongxiang Feng ◽  
Fei Liang ◽  
Wenhui Wang
Keyword(s):  
Chemical Analysis ◽  
Single Cell ◽  
Microfluidic Device ◽  
Single Cells ◽  
Cell Isolation ◽  
Cell Trapping ◽  
Analysis Techniques ◽  
Single Cell Trapping ◽  
Single Cell Isolation ◽  
Made In

Successful single-cell isolation is a pivotal technique for subsequent biological and chemical analysis of single cells. Although significant advances have been made in single-cell isolation and analysis techniques, most passive...

A microfluidic device for reversible environmental changes around single cells using optical tweezers for cell selection and positioning

Lab on a Chip ◽  
2010 ◽  
Vol 10 (5) ◽  
pp. 617-625 ◽  
Author(s):  
Emma Eriksson ◽  
Kristin Sott ◽  
Fredrik Lundqvist ◽  
Martin Sveningsson ◽  
Jan Scrimgeour ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Microfluidic Device ◽  
Environmental Changes ◽  
Single Cells ◽  
Cell Selection

Encapsulation of single cells on a microfluidic device integrating droplet generation with fluorescence-activated droplet sorting

2013 ◽  
Vol 15 (3) ◽  
pp. 553-560 ◽  
Author(s):  
Liang Wu ◽  
Pu Chen ◽  
Yingsong Dong ◽  
Xiaojun Feng ◽  
Bi-Feng Liu
Keyword(s):  
Microfluidic Device ◽  
Single Cells ◽  
Droplet Generation ◽  
Droplet Sorting

scholarly journals Microfluidic device for capture and isolation of single cells

2010 ◽  
Author(s):  
Alexander P. Hsiao ◽  
Kristopher D. Barbee ◽  
Xiaohua Huang
Keyword(s):  
Microfluidic Device ◽  
Single Cells

Capture and enumeration of mRNA transcripts from single cells using a microfluidic device

Lab on a Chip ◽  
2015 ◽  
Vol 15 (14) ◽  
pp. 2968-2980
Author(s):  
Matthew T. Walsh ◽  
Alexander P. Hsiao ◽  
Ho Suk Lee ◽  
Zhixia Liu ◽  
Xiaohua Huang
Keyword(s):  
Microfluidic Device ◽  
Single Cells ◽  
Direct Capture ◽  
Mrna Transcripts ◽  
Polyadenylated Mrna ◽  
Digital Counting

We report an integrated microfluidic device for direct capture and digital counting of polyadenylated mRNA molecules from single cells.

scholarly journals Microfluidic Techniques for Single-Cell Protein Expression Analysis

2006 ◽  
Vol 52 (6) ◽  
pp. 1080-1088 ◽  
Author(s):  
Ethan Fitzpatrick ◽  
Sterling McBride ◽  
Jonathan Yavelow ◽  
Saltanat Najmi ◽  
Peter Zanzucchi ◽  
...  
Keyword(s):  
Cell Surface ◽  
Protein Expression ◽  
Cancer Cells ◽  
Microfluidic Device ◽  
Single Cells ◽  
Surface Protein ◽  
Surface Proteins ◽  
Cell Protein ◽  
Surface Protein Expression ◽  
Mcf 7

Abstract Background: The analysis of single cells obtained from needle aspirates of tumors is constrained by the need for processing. To this end, we investigated two microfluidic approaches to measure the expression of surface proteins in single cancer cells or in small populations (<50 cells). Methods: One approach involved indirect fluorescence labeling of cell-surface proteins and channeling of cells in a microfluidic device past a fluorescence detector for signal quantification and analysis. A second approach channeled cells in a microfluidic device over detection zones coated with ligands to surface proteins and measured rates of passage and of retardation based on transient interactions between surface proteins and ligands. Results: The fluorescence device detected expression of integrin α5 induced by basic fibroblast growth factor (FGF-2) treatment in MCF-7 cells and that of Her-2/neu in SK-BR-3 cells compared with controls. Experiments measuring passage retardation showed significant differences in passage rates between FGF-2–treated and untreated MCF-7 cells over reaction regions coated with fibronectin and antibody to integrin α5β1 compared with control regions. Blocking peptides reversed the retardation, demonstrating specificity. Conclusions: Immunofluorescence detection in a microfluidic channel demonstrates the potential for assaying surface protein expression in a few individual cells and will permit the development of future iterations not requiring cell handling. The flow retardation device represents the first application of this technology for assessing cell-surface protein expression in cancer cells and may provide a way for analyzing expression profiles of single cells without preanalytical manipulation.

scholarly journals Integrated measurement of intracellular proteins and transcripts in single cells

Lab on a Chip ◽  
2018 ◽  
Vol 18 (21) ◽  
pp. 3251-3262 ◽  
Author(s):  
Alexander M. Xu ◽  
Qianhe Liu ◽  
Kaitlyn L. Takata ◽  
Sarah Jeoung ◽  
Yapeng Su ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Single Cells ◽  
Intracellular Proteins

Single cell coupled proteomic and transcriptomic measurements are captured on a scalable microfluidic device.

scholarly journals Design and Optimizations of a High-throughput Valve-based Microfluidic Device for Single Cell Compartmentalization and Analysis

2020 ◽  
Author(s):  
Jonathan Briones ◽  
Wilfred Espulgar ◽  
Shohei Koyama ◽  
Hyota Takamatsu ◽  
Eiichi Tamiya ◽  
...  
Keyword(s):  
Protein Expression ◽  
Single Cell ◽  
Microfluidic Device ◽  
High Throughput ◽  
Single Cells ◽  
Enzymatic Assay ◽  
Fluid Exchange ◽  
Genomics And Proteomics ◽  
Sealing Pressure ◽  
Cell Compartmentalization

Abstract The need for high throughput single cell screening platforms has been increasing with advancements in genomics and proteomics to identify heterogeneity, unique cell subsets or super mutants from thousands of cells within a population. For real-time monitoring of enzyme kinetics and protein expression profiling, valve-based microfluidics or pneumatic valving that can compartmentalize single cells is advantageous by providing on-demand fluid exchange capability for several steps in assay protocol and on-chip culturing. However, this technique is throughput limited by the number of compartments in the array. Thus, one big challenge lies in increasing the number of microvalves to several thousand that can be actuated in the microfluidic device to confine enzymes and substrates in picoliter volumes. This work explores the design and optimizations done on a microfluidic platform to achieve high-throughput single cell compartmentalization as applied to single-cell enzymatic assay for protein expression quantification. Design modeling through COMSOL Multiphysics was utilized to determine the circular microvalve’s optimized parameters, which can close thousands of microchambers in an array at lower sealing pressure. Multiphysical modeling results demonstrated the relationships of geometry, valve dimensions, and sealing pressure, which were applied in the fabrication of a microfluidic device comprising of up to 5000 hydrodynamic traps and corresponding microvalves. Comparing the effects of geometry, actuation media and fabrication technique, a sealing pressure as low as 0.04 MPa was achieved. Applying to single cell enzymatic assay, variations in granzyme B activity in Jurkat and human PBMC cells were observed. Improvement in the microfluidic chip’s throughput is significant in single cell analysis applications, especially in drug discovery and treatment personalization.

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