scholarly journals Slide Preparation for Mounting Samples for TissueCyte 1000 Sectioning and Imaging v2 (protocols.io.bdvni65e)

protocols.io ◽  
2020 ◽  
Author(s):  
Allen Institute
Keyword(s):  
Slide Preparation

Effect of Stain, Technician, Number of Spermatozoa Evaluated and Slide Preparation on Assessment of Spermatozoal Viability by Light Microscopy

1993 ◽  
Vol 13 (8) ◽  
pp. 449-455
Author(s):  
M.J. Hermenet ◽  
H.R. Sawyer ◽  
B.W. Pickett ◽  
R.P. Amann ◽  
E.L. Squires ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Slide Preparation

scholarly journals Eosinophil Counts in Mucosal Biopsies of the Ileum and Colon: Interobserver Variance Affects Diagnostic Accuracy

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Florian Hentschel ◽  
Anna Franziska Jansen ◽  
Marlis Günther ◽  
Roland Pauli ◽  
Stefan Lüth
Keyword(s):  
Staining Method ◽  
Sampling Site ◽  
Eosinophilic Gastroenteritis ◽  
Rare Entity ◽  
Endoscopic Findings ◽  
Slide Preparation ◽  
Microscopic Field ◽  
Histopathologic Findings ◽  
Very High ◽  
Eosinophil Counts

Primary eosinophilic gastroenteritis and colitis (EGE) is a rare entity with unspecific clinical and endoscopic findings. Validated histopathologic criteria for confirming the diagnosis are lacking, because numeric values for normal or elevated concentrations of eosinophils in mucosal biopsies are varying between observers. To quantify this interobserver variance, we had the same set of 30 slides of eosinophilic-rich mucosal biopsies from the ileum and colon systematically reviewed by a panel of six independent pathologists, each with more than a ten-year experience in the field. Using a highly standardized biopsy and slide preparation protocol, we ruled out any influence by the preparation, the patient, the endoscopist, the endoscopes and calipers used, the sampling site, the fixation and staining method, and the microscopic field sizes. Still, all numeric results differed between pathologists up to a factor greater than 30. Calculated positive or negative diagnosis of EGE differed up to a factor greater than 8. A theoretical incidence for EGE calculated from these numbers differed by a factor greater than 1500. We conclude that eosinophil counts in mucosal biopsies from the lower gastrointestinal tract are subject to a very high interobserver variance. Until further research provides objective and validated methods for standardization, all epidemiologic numbers derived from histopathologic findings may have to be questioned. When diagnosing individual patients with EGE, overall morphologic picture together with clinical and endoscopic findings is more important than numeric eosinophil count.

Metaphase position of an interchange quadrivalent of Allium triquetrum

1988 ◽  
Vol 90 (3) ◽  
pp. 493-499
Author(s):  
GEOFFREY K. RICKARDS ◽  
WENDY A. BAKER
Keyword(s):  
Linear Array ◽  
Metaphase Plate ◽  
Real Data ◽  
Slide Preparation ◽  
Metaphase I

The position of an interchange quadrivalent at metaphase I of Allium triquetrum was modelled so as to simulate the original placement of the quadrivalent in the spindle and the preparation of linear spreads through squashing. In this way an expected distribution for the quadrivalent in linear spreads was generated. The procedure used polar views of metaphase I to which the quadrivalent was assigned pairs of positions normally occupied by bivalents. The positions of the bivalents and assigned quadrivalent were then into a linear array and analysed as ‘real’ data. Comparisons with observed distributions showed that a general bias in favour of marginal placement of the quadrivalent in the linear array is expected; and also showed that the quadrivalent is positioned non-randomly in the metaphase plate prior to slide preparation.

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