A Modified Method of Coal Maceration and a Simple Technique for Slide Preparation

1964 ◽  
Vol 10 (4) ◽  
pp. 486 ◽  
Author(s):  
Howard W. Lee
Keyword(s):  
Simple Technique ◽  
Slide Preparation ◽  
Modified Method

A METHOD FOR REARING VARIOUS SPECIES OF SOCIAL WASPS OF THE GENUS POLISTES (HYMENOPTERA: VESPIDAE) UNDER CONTROLLED CONDITIONS

1977 ◽  
Vol 109 (7) ◽  
pp. 1013-1015 ◽  
Author(s):  
David L. Gibo
Keyword(s):  
Social Behaviour ◽  
Simple Technique ◽  
Mass Rearing ◽  
Colony Growth ◽  
Social Wasps ◽  
Colony Founding ◽  
Rearing Method ◽  
Modified Method ◽  
Controlled Conditions ◽  
Caste Differences

Wasps of the genus Polistes have a number of characteristics that make them particularly suitable for studies on insect social behaviour. The females construct nests with exposed combs, have a reasonably complex social behaviour, and usually lack obvious morphological caste differences. In addition, the adults of many species establish colonies as groups of siblings (foundress associations), a method which is intermediate between colony founding by swarming, and colony founding by individual queens (Wilson 1971). Because of these characteristics, Polistes have been of interest to biologists who are concerned with the evolution of social behaviour in insects (e.g. Alexander 1974, Gibo 1974, Lin and Michener 1972, West 1967, West Eberhard 1969, 1975). Consequently, there is a need for a simple technique for rearing various species of Polistes under controlled conditions. Gibo (1974) briefly described modifications of Gillaspy's (1971) rearing method for P. apachus which made mass rearing of P. fascatus possible. This note describes the modified method in greater detail, and includes improvements that have resulted in increased yield and greater synchrony of colony growth. The improvements have been developed over 3 years and the method has been used to rear more than 100 colonies of various species of Polistes, including P. fuscatus, P. apachus, and P. dorsalis.

scholarly journals Simple Modified Method for Fungal Slide Preparation

2013 ◽  
Vol 54 (2) ◽  
pp. 141-146 ◽  
Author(s):  
Shigeru Fujita
Keyword(s):  
Slide Preparation ◽  
Modified Method

A Simple Technique for Obtaining a Colon Biopsy Specimen per Rectum

1962 ◽  
Vol 42 (2) ◽  
pp. 157-159 ◽  
Author(s):  
Eugene J. Gangarosa ◽  
William R. Beisel
Keyword(s):  
Biopsy Specimen ◽  
Simple Technique ◽  
Colon Biopsy

A SIMPLE TECHNIQUE FOR MEASUREMENT OF CORNEAL THICKNESS

1998 ◽  
Vol 21 (4) ◽  
pp. 109-114
Author(s):  
C Albarrán ◽  
R Montés-Micó ◽  
A M Pons ◽  
A Gené ◽  
A Lorente
Keyword(s):  
Simple Technique ◽  
Corneal Thickness

Proposal for Objective Evaluation of the Performance of Various Functional APC-resistance Tests in Genotyped Patients

1997 ◽  
Vol 78 (05) ◽  
pp. 1360-1365 ◽  
Author(s):  
G Freyburger ◽  
S Javorschi ◽  
S Labrouche ◽  
P Bernard
Keyword(s):  
Protein S ◽  
Objective Evaluation ◽  
Original Method ◽  
Screening Methods ◽  
Factor Xii ◽  
Factor V ◽  
Likelihood Ratios ◽  
Modified Method ◽  
Apc Resistance ◽  
Two Parameters

SummaryThe aim of the present study was to evaluate the relative performance of five screening methods for APC resistance caused by the factor V:Q506 mutation: the original method Coatest® APC™ Resistance Chromogenix, a modified method using the same reagents but a predilution 1+4 of the plasma in a factor V deficient plasma from Stago (Stago deficient V) or from Chromogenix (V-DEF Plasma), the Coatest® APC™ Resistance V (Chromogenix), and Accelerimat™ from bioMérieux. Normalization was done against a pool of normal plasmas for the methods from Chromogenix. The study included 350 subjects, 219 were genotyped (174 FV:R506R, 42 FV:Q506R, 3 FV:Q506Q) and most of them were assessed by more than one method. Uncertainty in predicting the FV genotype was evaluated by statistical analysis, which provided a way to quantitate the performance of the different diagnostic approaches. Performance of each test was evaluated by its sensitivity, specificity, R.O.C. curves, positive and negative likelihood ratios (LR), and the overall performance was determined by two parameters derived from the LR curves : the maximum LR value obtained at the crossover of the two curves, and the distance between the two curves for LR = 10. Coatest® APC™ Resistance V and Accelerimat™ were proven to be the methods most able to discriminate for factor V:Q506, while normalization was not shown to improve the screening performance. The original method from Chromogenix was confirmed to undergo many influences (factor XII, PAI-1, thrombin- antithrombin complexes, antithrombin III, hematocrit). Although a very good improvement was provided by the newest methods, they were shown to be influenced by protein S and/or factor V levels in the sample plasma.

Outcome of Buccal Mucosal Graft Urethroplasty in Long Segment Anterior Urethral Stricture

2020 ◽  
Vol 19 (2) ◽  
pp. 64-68
Author(s):  
Mrinmoy Biswas ◽  
Sudip Das Gupta ◽  
Mohammed Mizanur Rahman ◽  
Sharif Mohammad Wasimuddin
Keyword(s):  
Urethral Stricture ◽  
Flow Rate ◽  
Surgical Outcome ◽  
Simple Technique ◽  
Anterior Segment ◽  
Maximum Flow ◽  
Anastomotic Site ◽  
Buccal Mucosal Graft ◽  
Male Patients

Objective: To assess the success of BMG urethroplasty in long segment anterior urethral stricture. Method: From January 2014 to December 2015, twenty male patients with long anterior segment urethral stricture were managed by BMG urethroplasty. After voiding trial they were followed up at 3 month with Uroflowmetry, RGU & MCU and PVR measurement by USG. Patients were further followed up with Uroflowmetry and PVR at 6 months interval.Successful outcome was defined as normal voiding with a maximum flow rate >15ml /sec and PVR<50 ml with consideration of maximum one attempt of OIU after catheter removal. Results: Mean stricture length was 5.2 cm (range 3-9 cm) and mean follow-up was 15.55 months (range 6-23 months). Only two patients developed stricture at proximal anastomotic site during follow-up. One of them voided normally after single attempt of OIU. Other one required second attempt of OIU and was considered as failure (5%). Conclusion: BMG urethroplasty is a simple technique with good surgical outcome. Bangladesh Journal of Urology, Vol. 19, No. 2, July 2016 p.64-68

QUANTITATIVE ESTIMATION OF URINARY PREGNANETRIOL, PREGNANETRIOLONE, TETRAHYDRO S AND Δ5-PREGNENETRIOL IN THE INVESTIGATION OF ADRENOCORTICAL FUNCTION

1969 ◽  
Vol 60 (4) ◽  
pp. 657-668 ◽  
Author(s):  
Frances J. Thomas ◽  
A. W. Steinbeck
Keyword(s):  
Congenital Adrenal Hyperplasia ◽  
Cushing’S Syndrome ◽  
Adrenocortical Carcinoma ◽  
Quantitative Estimation ◽  
Cushing's Syndrome ◽  
Adrenocortical Function ◽  
Adrenal Hyperplasia ◽  
Ectopic Acth ◽  
Modified Method ◽  
Idiopathic Hirsutism

ABSTRACT A modified method for the estimation of urinary pregnanetriol, pregnanetriol, pregnanetriolone, Δ5-pregnenetriol and tetrahydro S was investigated. The steroids, separated by chromatography, were measured quantitatively, tetrahydro S by reaction with blue tetrazolium and the other three as acetaldehydogenic substances. The excretion of these steroids was studied in suspected instances of adrenal and/or ovarian disease. Urinary pregnanetriol and tetrahydro S levels were normal in Cushing's syndrome, secondary adrenocortical carcinoma, the Stein-Leventhal syndrome, idiopathic hirsutism and hypertension. Tetrahydro S was doubtfully elevated in an instance of ectopic ACTH Cushing's syndrome. Pregnanetriol excretion was elevated in untreated cases of congenital adrenal hyperplasia and after treatment in some. Pregnanetriolone was found in all patients with congenital adrenal hyperplasia, including those receiving cortisone. Pregnanetriolone was also detected in Cushing's syndrome, secondary adrenocortical carcinoma, the Stein-Leventhal syndrome, ectopic ACTH Cushing's syndrome and suspected congenital adrenal hyperplasia. Δ5-pregnenetriol was present as a glucuronide in the Stein-Leventhal syndrome and idiopathic hirsutism. The diagnostic implications of these results are discussed.

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