Identification of molecular markers in soybean comparing RFLP, RAPD and AFLP DNA mapping techniques

1996 ◽  
Vol 14 (2) ◽  
pp. 156-169 ◽  
Author(s):  
Jhy-Jhu Lin ◽  
Jonathan Kuo ◽  
Jin Ma ◽  
James A. Saunders ◽  
Hunter S. Beard ◽  
...  
HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 693a-693
Author(s):  
V. Meglic ◽  
R.T. Chetelat

The C.M. Rick Tomato Genetic Resources Center (TGRC) is a genebank of wild relatives, monogenic mutants, and miscellaneous genetic stocks of tomato. The wild species group includes representatives of all nine Lycopersicon spp., as well as four related Solanum species. One of the roles of the TGRC has been to foster the use of the widest available gene pool for tomato researchers. The wild nightshade Solanum lycopersicoides possesses a number of potentially useful traits, but has been untapped by breeders because of sterility and incompatibility barriers. We are using molecular markers to identify alien chromosomal segments introgressed from S. lycopersicoides into tomato. This project involves development of RFLP, RAPD, and isozyme marker linkage maps and their use in selection of homozygous segmental substitutions in backcross inbred progenies. In this fashion, a large proportion of the S. lycopersicoides genome has been integrated into the cultivated tomato. This study has also provided information on the nature of sterility and novel variation in hybrid derivatives.


As we are moving forward into the modern era of science, several new technologies have revolutionized various branches of science. Techniques of biodiversity conservation, fish biology etc. has also adapted to modern techniques. For a long time, most of the researches in taxonomy, including fisheries science were based on morphology and traditional methods. After the decade of 90’s, slowly severalmolecular markers like RFLP, RAPD, SNP’s etc. made inroad into taxonomy and fisheries. Molecular markers have several applications in the field of livestock improvement and understanding population dynamics to name a few. Since the 2004, a specific molecular marker, generally known as DNA Barcoding for species identification, came up. This molecular marker is a part of mitochondrial genome that encodes for Cytochrome C Oxidase Unit I (also called as COX or COI). It is advantageous because it has been tested across several animal species and it can differentiate species very well. This marker has also been used as a forensic tool to identify the species. In the current paper, we have used this molecular marker to decode evolution of native fishes of Garhwal Himalayan region. Over 350 barcodes were developed and these barcodes were used to for phylogenetic analysis.


1998 ◽  
Vol 97 (7) ◽  
pp. 1083-1089 ◽  
Author(s):  
A. Spada ◽  
E. Caporali ◽  
G. Marziani ◽  
P. Portaluppi ◽  
F. M. Restivo ◽  
...  

Swiss Surgery ◽  
2001 ◽  
Vol 7 (6) ◽  
pp. 243-248
Author(s):  
Scheunemann ◽  
Hosch ◽  
Kutup ◽  
Izbicki

Die Einführung von immunhisto-/zytochemischen und molekularbiologischen bzw. zytogenetischen Methoden in der onkologische Forschung hat trotz vieler nach wie vor bestehender offener Fragen insgesamt zu einem besseren Verständnis der genetischen Ursachen der Tumorentstehung geführt. Darüber hinaus ergaben sich in verschiedenen Studien Hinweise für eine prognostische Relevanz von bestimmten (zyto)genetischen Veränderungen bzw. residualen Tumorzellen in Lymphknoten oder Knochenmark. Bevor diese Untersuchungen jedoch in den klinischen Alltag Einzug halten und therapeutische Konsequenzen abgeleitet werden können, sind weitere prospektive Studien mit groáen Patientenfallzahlen sowie vereinheitlichte und methodologisch praktikable Untersuchungstechniken zu fordern.


2014 ◽  
Vol 226 (03) ◽  
Author(s):  
T Vu-Han ◽  
MC Frühwald ◽  
M Hasselblatt ◽  
F Oyen ◽  
T Obser ◽  
...  

2000 ◽  
Vol Volume 26 (Number 01) ◽  
pp. 023-028 ◽  
Author(s):  
Katsumi Deguchi ◽  
Akira Deguchi ◽  
Hideo Wada ◽  
Seiko Murashima

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