scholarly journals Laboratory evaluation of the effect of the entomopathogenic fungi, Hirsutella thompsonii and Paecilomyces fumosoroseus, against the citrus brown mite, Eutetranychus orientalis (Acari: Tetranychidae

2016 ◽  
Vol 51 (No. 1) ◽  
pp. 39-45 ◽  
Author(s):  
H.M. El-Sharabasy
Keyword(s):  
Entomopathogenic Fungi ◽  
Laboratory Evaluation ◽  
Paecilomyces Fumosoroseus ◽  
Hirsutella Thompsonii

scholarly journals SUSCETIBILIDADE DE NINFAS DE DIAPHORINA CITRI A FUNGOS ENTOMOPATOGÊNICOS

2009 ◽  
Vol 76 (2) ◽  
pp. 297-302
Author(s):  
L.F.L. Padulla ◽  
S.B Alves
Keyword(s):  
Beauveria Bassiana ◽  
Metarhizium Anisopliae ◽  
Paecilomyces Fumosoroseus ◽  
Diaphorina Citri ◽  
Murraya Paniculata ◽  
Hirsutella Thompsonii

RESUMO Avaliou-se a patogenicidade de diversas espécies de fungos entomopatogênicos a ninfas de 2o a 4o ínstares do psilídeo Diaphorina citri. Foram feitos bioensaios com Beauveria bassiana, Metarhizium anisopliae, Lecanicillium muscarum, L. longisporum, Paecilomyces fumosoroseus, P. farinosus, Syngliocladium sp. na concentração de 5 x 107 conídios/mL para cada patógeno, com exceção de Hirsutella thompsonii que foi aplicado na concentração de 2,8 x 107 conídios/mL. Utilizaram-se mudas de murta, Murraya paniculata, infestadas com as ninfas do inseto que foram pulverizadas com as suspensões conidiais dos patógenos. Os fungos B. bassiana, M. anisopliae, H. thompsonii, L. muscarum e P. fumosoroseus foram patogênicos para as ninfas do psilídeo. O isolado Esalq-PL63, de B. bassiana, causou mortalidade de aproximadamente 72% das ninfas, sete dias após a inoculação. Constatou-se que B. bassiananão conseguiu completar o desenvolvimento no corpo do hospedeiro, uma vez que a fase de conidiogênese não ocorreu. Mesmo assim, o isolado Esalq-PL63 pode ser considerado um promissor agente de controle microbiano de ninfas de D. citri por causar altos índices de mortalidade.

Comparing methods of preservation for cultures of entomopathogenic fungi

2002 ◽  
Vol 80 (10) ◽  
pp. 1126-1130 ◽  
Author(s):  
Claudia C López Lastra ◽  
Ann E Hajek ◽  
Richard A Humber
Keyword(s):  
Aedes Aegypti ◽  
Silica Gel ◽  
Entomopathogenic Fungi ◽  
Mineral Oil ◽  
Fungal Species ◽  
Deionized Water ◽  
Paecilomyces Fumosoroseus ◽  
Culture Viability ◽  
Leptolegnia Chapmanii ◽  
And Storage

Nine species of entomopathogenic fungi were tested for viability after they had been stored with deionized water, mineral oil, or silica gel or frozen at –20 or –80°C. Species tested included members of the Hyphomycetes, Entomophthorales, Trichomycetes, and Oomycetes. The fungal cultures were maintained up to 1.5 years and were checked at 3, 6, 12, and 18 months. For all species evaluated, Paecilomyces fumosoroseus demonstrated the best results, surviving through 18 months when stored with water or mineral oil and when frozen at –80°C. For the majority of other fungal species tested, except the trichomycete Smittium culisetae and oomycete Leptolegnia chapmanii, freezing at –80°C was the best storage method and storage with silica gel was the worst. In addition to culture viability, infectivity against Aedes aegypti larvae was evaluated after 18 months of storage for L. chapmanii and S. culisetae. The simplest and least expensive methods, using water or mineral oil, were the only successful methods for maintaining viability and infectivity of L. chapmanii and S. culisetae.Key words: entomopathogenic fungi, preservation, storage, viability.

scholarly journals Influence of pesticides used potatoes control on the growth of entomopathogenic fungi isolated from potatoes fields

2013 ◽  
Vol 48 (2) ◽  
pp. 65-73
Author(s):  
Ryszard Miętkiewicz ◽  
Anna Sapiecha
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Galleria Mellonella ◽  
Paecilomyces Fumosoroseus ◽  
Copper Oxychloride ◽  
Growth Of Fungi

The growth of <em>Metarhizium anisopliae, M.flavoviridae</em> and <em>Paecilomyces fumosoroseus</em> was estimated on Sabouraud's medium to which insecticides and herbicides were added in three doses: A - 10 times higher from recommended field dose, B - as recommended field dose, C - 10 timer lower than recommended. Fungicides were used in B and C doses as well as in dose D - 100 times lower than recomended one. The fungi were obtained from soil under potatoes using <em>Galleria mellonella</em> as bait insect. Chlorothalonil and copper oxychloride were chosen from fungicides, linuron, MCPA, fluazifop-P-butyl and dikwat - from herbicides and deltamethrin, teflubenzuron and fozalon from insecticides. The growth of both species of <em>Metarhizium</em> was stronger inhibited than of <em>Paecilomyces fumosoroseus</em> by fungicides however colonies of <em>Metarhizium</em> always overpassed 50% controls colonies apart from <em>M. flavoviridae</em> on medium with copper oxychloride at concentration B. Herbicide linuron was more toxic to fungi than fungicides. <em>M. anisopliae</em> and <em>M. fluvoviridae</em> did not grow on medium containing dose A and dose B this herbicide but the colonies of <em>P. fumosoroseus</em> at dose B did not overpass 20% of controlled ones. MCPA and fluazifop-P-butyl inhibited fungal colonies in approximated way. On the medium with these herbicides in concentration A fungal colonies were strongly inhibited and the growth of fungi on medium with MCPA at this concentration appeared not before 5 days after inoculation. Dikwat in dose A strongly inhibited the growth of <em>M. anisopliae</em> but in remaining combinations growth of fungal colonies was similar to controlled ones. Fozalon, among insecticides, inhibited the growth of inwestigated fungi strongest. On the medium containing this insecticide in dose A all fungi did not grow, and in dose B colonies of both species of <em>Metarhizium</em> did not overpass 40% of controlled ones. Deltamethrin in dose A and B inhibited the growth of <em>M. anisopliae</em> and <em>M. flavoviridae</em>, but teflubenzuron did not inhibit growth of entomopathogenic fungi at all concentrations.

scholarly journals 008 Virulence of Entomopathogenic Fungi (Hyphomycetes) against Anthonomus fulvipes (Coleoptera:Curculionidae) in Organically Grown Barbados Cherry Trees: Laboratory and Field Trials

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 389B-389
Author(s):  
R. Lezama-Gutierrez ◽  
J. Molina-Ochoa ◽  
O. Rebolledo-Dominguez ◽  
M. Gonzalez Ramirez ◽  
M. Lopez-Edwards
Keyword(s):  
Entomopathogenic Fungi ◽  
Field Experiments ◽  
Microbial Control ◽  
Field Evaluation ◽  
Field Trials ◽  
Paecilomyces Fumosoroseus ◽  
High Virulence ◽  
Fruit Damage ◽  
Organically Grown ◽  
Cherry Trees

Virulence of several isolates of the entomopathogenic fungi Beauveria bassiana (Bals.) Vuill., Metarhizium anisopliae (Metsch.) Sor. and Paecilomyces fumosoroseus (Wize) Brown & Smith were evaluated on adult weevils of Anthonomus fulvipes Boheman at dose of 108 spores/mL under laboratory conditions. The study was complemented by testing one isolate each of M. anisopliae and P. fumosoroseus against adult weevils on organically grown Barbados cherry trees at dose of 2 × 1015 spores/ha. All fungi species showed high virulence against A. fulvipes adults, with mortality ranging from 92% to 100%. LT50 values varied 2.7 to 4.8 d. The M. anisopliae isolate 10, and the P. fumosoroseus isolate 1 were selected for field evaluation because laboratory insect cadavers presented the best sporulation. After applying the fungi to the trees, total weevil captures were 38, 56, and 100 for the P. fumosoroseus, M. anisopliae, and the check (untreated) plots, respectively. Statistical differences in fruit damage were detected among treatments: M. anisopliae and P. fumosoroseus treatments showed an average of 50% of undamaged fruits, whereas in the control plots presented 36% of undamaged fruits. Laboratory and field experiments suggest that entomopathogenic fungi have a potential as microbial control agents against the weevil A. fulvipes in organically grown Barbados cherry trees.

Genetic variability and phylogeny inferred from random amplified polymorphic DNA data reflect life strategy of entomopathogenic fungi

2000 ◽  
Vol 78 (9) ◽  
pp. 1150-1155 ◽  
Author(s):  
Miroslav Oborník ◽  
Marek Klíc ◽  
Lukáš Zizka
Keyword(s):  
Genetic Variability ◽  
Entomopathogenic Fungi ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Distances ◽  
Major Influence ◽  
Life Strategy ◽  
Verticillium Lecanii ◽  
Paecilomyces Fumosoroseus ◽  
Neighbor Joining

Our analysis of the genetic variability and phylogenetic relationships among the isolates of mitosporic entomopathogenic fungi Paecilomyces fumosoroseus (Wize) Brown & Smith (10 isolates), Paecilomyces farinosus (Holm ex S.F. Gray) Brown & Smith (3 isolates), Paecilomyces lilacinus (Thom) Samson (1 isolate), and Verticillium lecanii (Zimmerman) Viegas (6 isolates) was based on the use of random amplified polymorphic DNA (RAPD) markers. Neighbor-joining analysis characterized P. fumosoroseus and V. lecanii as monophyletic taxa. The analysis also supported monophyly of the genus Paecilomyces. We have used the distribution of genetic distances to demonstrate the genetic variability within studied species, in Paecilomyces spp. (Hyphomycetes) and Aschersonia spp. (Coelomycetes) in particular. We conclude that the RAPD-based analysis is useful for the reconstruction of intraspecific and interspecific relationships of the entomopathogenic fungi. Moreover, it can be employed to study the intergeneric relationships, especially in the case of closely related genera of the hyphomycetous entomopathogenic fungi. We propose that RAPD-based phylogeny and genetic variability reflect their life strategy. In our opinion, the mobility of spores represents a factor with major influence on the evolution of these entomopathogenic fungi.Key words: Paecilomyces, Verticillium, Aschersonia, RAPD, phylogeny, genetic variability.

Entomopathogenic fungi and nematodes against larvae of the chestnut weevil, Curculio elephas (Coleoptera: Curculionidae): a laboratory evaluation

2017 ◽  
Vol 64 (4) ◽  
pp. 287-293 ◽  
Author(s):  
Giulia Torrini ◽  
Claudia Benvenuti ◽  
Francesco Binazzi ◽  
Leonardo Marianelli ◽  
Francesco Paoli ◽  
...  
Keyword(s):  
Entomopathogenic Fungi ◽  
Laboratory Evaluation ◽  
Curculio Elephas
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