scholarly journals Genetic variation of Chilo suppressalis Walker (Lepidoptera: Pyralidae) populations in Guilan and west of Mazandaran provinces analysed with RAPD markers 

2014 ◽  
Vol 50 (No. 1) ◽  
pp. 26-35 ◽  
Author(s):  
A. Farahpour Haghani ◽  
R. Hosseini ◽  
A.A. Ebadi ◽  
A. Aalami
Keyword(s):  
Genetic Variation ◽  
Rapd Markers ◽  
Population Group ◽  
Stem Borer ◽  
Pcr Analysis ◽  
Chilo Suppressalis ◽  
Mazandaran Province ◽  
The West ◽  
Population Genetic Variation ◽  
High Level

Genetic variation of striped stem borer Chilo suppressalis populations in Guilan and Mazandaran provinces in Iran was studied in 2010 by means of RAPD markers. Collected 45 samples from 19 locations (representing 17 counties in Guilan province and 2 counties in Mazandaran province) were classified into four groups of populations: three population groups corresponding to the west, centre, and east of Guilan and one population group from the west of Mazandaran. RAPD PCR analysis showed that Chilo suppressalis populations in Mazandaran are genetically different from the Guilan populations while the western populations of Guilan are genetically separated from the two other populations. The central and the eastern populations of Guilan exhibit some similarities with those of Mazandaran and the similarities are more obvious between the eastern populations of Guilan and the western population of Mazandaran. The population from the centre of Guilan showed the highest level of intra-population genetic variation possibly due to wider rice cultivated area and/or due to higher sample size. The results showed a high level of genetic variation in Chilo suppressalis populations in the two provinces in Iran. In spite of the model species is a widely distributed pest, the study indicated that the samples originated in populations with a different genetic make-up.

scholarly journals Genetic diversity of rice stem borer (Chilo suppressalis Walker) from Northern Iran and comparison with other countries

2016 ◽  
Vol 48 (3) ◽  
pp. 360 ◽  
Author(s):  
M. Shayanmehr ◽  
E. Yoosefi-Lafooraki
Keyword(s):  
Genetic Diversity ◽  
Stem Borer ◽  
Chilo Suppressalis ◽  
Mazandaran Province ◽  
Northern Iran ◽  
Low Genetic Diversity ◽  
High Gene ◽  
Major Pest ◽  
Different Populations ◽  
Low Levels

Rice striped stem borer, <em>Chilo suppressalis</em> Walker (Lepidoptera: Crambidae) is considered the major pest of rice in Iran. Because of the serious damage on rice in Northern Iran, the present study was conducted to investigate genetic diversity within populations of <em>C. suppressalis</em>, from Mazandaran using a template of cytochrome oxidase I gene, 750 bps, (<em>COI</em>). Later the haplotypes from Iran were compared with those found in other countries. According to the results of this study, there is very low genetic diversity (two haplotypes) among different populations of this pest in populations of Northern Iran. The genetic similarity and low levels of genetic diversity of these populations suggest that the pest colonization occurred relatively recently and there is high gene flow between these populations of the province. In addition, haplotypes of Mazandaran province are different with those found in other countries. The similarity of Iranian population (Simorgh) with one population from China indicated that China might be the origin of <em>C. suppresalis</em>.

scholarly journals GENETIC VARIATION AND RELATEDNESS AMONG HIGH YIELDING RICE VARIETIES (Oryza sativa L.) REVEALED BY RAPD MARKERS

2008 ◽  
Vol 21 (1) ◽  
pp. 07-14
Author(s):  
F. Easmin ◽  
M. S. Rahman ◽  
M. S. Islam ◽  
M. A. Samad ◽  
M. S. Alam
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Rapd Markers ◽  
Oryza Sativa L ◽  
Gene Diversity ◽  
Chain Reaction ◽  
Rice Varieties ◽  
Upgma Dendrogram ◽  
Polymerase Chain ◽  
High Level

Genetic variation is a principal concern for the plant breeders. Genetic variation and relationship among high yielding rice varieties viz. Binadhan 4, Binadhan 5, Binadhan 6, Binasail, BRRI dhan28 and BRRI dhan29 were analyzed using four decamer random primers. Polymerase Chain Reaction (PCR) amplified 22 RAPD markers, of which 18 (81.82%) were polymorphic. The proportion of polymorphic loci and the gene diversity values were 59.09% and 0.25 for the Binadhan 4; 59.09% and 0.21 for Binadhan 6; 54.55% and 0.23 for Binasail; 54.55% and 0.19 for BRRI dhan29; 50.00% and 0.19 for Binadhan 5 and 45.45% and 0.18 for BRRI dhan28, respectively. The coefficient of gene differentiation (Gst) across all loci was calculated as 0.35 reflecting the existence of high level of genetic variation among the six modern rice varieties. UPGMA dendrogram based on Nei’s genetic distance segregated the six high yielding rice varieties into two clusters: all four mutant varieties viz. Binadhan 4, Binadhan 5, Binadhan 6 and Binasail formed one cluster and two varieties of BRRI grown in boro season, BRRI dhan28 and BRRI dhan29 grouped together in another cluster. Among the mutants, two boro season varieties, developed from the same parent, Binadhan 5 and Binadhan 6 grouped together with genetic distance of 0.10. Therefore, RAPD offer a reliable method to evaluate genetic variation and relatedness among the high yielding rice varieties.DOI: http://dx.doi.org/10.3329/bjpbg.v21i1.17042

scholarly journals Genetic Variability Assay of Different Natural and Hatchery Populations of Rohu (Labeo rohita) in Bangladesh

2015 ◽  
Vol 9 (1) ◽  
pp. 30-36
Author(s):  
Shikder Saiful Islam ◽  
Md. Saifuddin Shah ◽  
Foyez Ibn Shams ◽  
Md. Rayhan Ali ◽  
Md. Lifat Rahi
Keyword(s):  
Genetic Variation ◽  
Genetic Variability ◽  
Labeo Rohita ◽  
Genetic Distances ◽  
Raw Material ◽  
Group Method ◽  
Pair Group ◽  
Fish Samples ◽  
Population Genetic Variation ◽  
High Level

The level of genetic variation determines the genetic status and provides the raw material for selective improvement of a stock. Randomly amplified polymorphic DNA (RAPD) technique was used to assess the genetic variability of 7 different natural (2) and hatchery (5) populations of Indian Major Carp, Labeo rohita (Rohu) in Bangladsh. In total, 140 fish samples were collected (20 from each of the populations). Genomic DNA was extracted from the muscle tissue, and 5 different oligonucleotide primers were used which revealed 80% polymorphic DNA bands. The polymorphic loci proportions were 0.71, 0.75, 0.75, 0.85, 0.84, 0.86 and 0.89 for Ma-Fatema hatchery, Chowdhuri hatchery, Niribili hatchery, Sonali hatchery, Kapotakha hatchery, the Halda river and the Baluhor Baor populations respectively. The pair-wise population differentiation (FST) values indicated a high level of genetic variation between different populations. The Unweighted Pair Group Method of Arithmetic Mean (UPGMA) dendogram based on Nei’s genetic distances also revealed high level of inter-population genetic variation among the populations. The populations were segregated into two groups: the Halda River and Baluhar Baor hatchery in one group and Kapotakha, Ma-Fatema, Chowdhuri, Niribili and Sonali hatcheries in another group. Overall, RAPD results clearly indicate the reduced genetic quality of the hatchery seeds.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11923 International Journal of Life Sciences Vol.9(1) 2015 30-36

scholarly journals RAPD Profile Analysis of Single and Multigrain Aman Rice (Oryza sativa L.) Varieties Available in Bangladesh

2017 ◽  
Vol 27 (2) ◽  
pp. 195-205
Author(s):  
Shawon Mitra ◽  
Tahmina Islam ◽  
RH Sarker ◽  
M Imdadul Hoque
Keyword(s):  
Genetic Variation ◽  
Molecular Typing ◽  
Rapd Markers ◽  
Profile Analysis ◽  
Oryza Sativa L ◽  
Population Analysis ◽  
Rice Varieties ◽  
Rapd Profile ◽  
Single Grain ◽  
High Level

Genetic variation in 14 local Aman rice varieties of Bangladesh including single grain and multigrain rice was investigated at the DNA level by RAPD molecular typing. Fifteen random primers were initially screened against DNA from five individuals that generated highly reproducible RAPD fragments, which were then subjected to further population analysis. A total 191 RAPD fragments were generated with these primers and 153 (80.1%) were polymorphic, which indicated high level of polymorphism existed within these populations. The size of amplified fragments ranged between 450 and 6000 bp. Pair‐wise distance estimated the range of 0.03 to 0.59 between single and multigrain Aman rice varieties. Results illustrated the potential of RAPD markers to distinguish improved varieties and grain specificity at DNA level.Plant Tissue Cult. & Biotech. 27(2): 195-205, 2017 (December)

Genetic variability and phytogeography of Miscanthus sinensis var. condensatus, an apomictic grass, based on RAPD fingerprints

2000 ◽  
Vol 78 (10) ◽  
pp. 1262-1268 ◽  
Author(s):  
Chang-Hung Chou ◽  
Yu-Chung Chiang ◽  
Tzen-Yuh Chiang
Keyword(s):  
Genetic Variation ◽  
Rapd Markers ◽  
High Salinity ◽  
Random Amplified Polymorphic Dna ◽  
Miscanthus Sinensis ◽  
Ryukyu Islands ◽  
Banding Patterns ◽  
High Level ◽  
The Cost ◽  
Two Populations

DNA fingerprinting using random amplified polymorphic DNA (RAPD) markers was employed to investigate the genetic variation within and among populations of Miscanthus Anderss. sinensis var. condensatus (Hack.) Makino, an apomictic grass distributed along the coasts of Taiwan and Ryukyu Islands. A total of 250 plants from three Taiwanese populations (Southeast Coast, Orchid Islet, and Green Islet) and two populations from Ryukyu (Ishigaki and Amami-O-Shima Islets) were sampled. The amplified products of 40 random primers showed monomorphic banding patterns within all populations as well as among the three populations from Taiwan. Low genetic variation (with only two polymorphic loci), but significant differentiation, was detected between populations from Taiwan and Ryukyu (ΦCT = 0.864) and between populations (ΦST = 1.0) from Ishigaki and Amami-O-Shima Islets. In contrast, a high level of variation was exhibited in the outcrossing Miscanthus sinensis var. glaber (Nakai) Li. In addition to apomictic reproduction, low genetic variation across populations of M. sinensis var. condensatus may be a result of high salinity acting as a selective agent. With the cost of reduced genetic heterogeneity, apomixis may have provided a mechanism for avoiding the transmission of endophytic fungi. The phytogeographic pattern of M. sinensis var. condensatus, as reflected by the RAPD data, likely represents isolation between Taiwan and Ryukyu since the mid-Pleistocene.Key words: apomixis, Miscanthus sinensis var. condensatus, phytogeography, population differentiation, RAPD, system of mating.

Molecular phylogeny of mangroves VI. Intraspecific genetic variation in mangrove species Excoecaria agallocha L. (Euphorbiaceae)

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 110-115 ◽  
Author(s):  
M Lakshmi ◽  
M Parani ◽  
Nivedita Ram ◽  
Ajay Parida
Keyword(s):  
Genetic Variation ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
The West ◽  
Mangrove Species ◽  
Excoecaria Agallocha ◽  
Polymorphic Restriction ◽  
High Level ◽  
Key Words Mangroves ◽  
Mean Similarity

Genomic DNA from 84 individuals of Excoecaria agallocha from seven mangrove populations were analysed for random amplified polymorphic DNAs (RAPDs) using 16 random 10-mer primers. Polymorphism within populations varied from 20% to 31%. At the interpopulation level, 111/149 (74%) of RAPDs were polymorphic. Restriction fragment length polymorphism (RFLP) analysis of 21 individuals (3 individuals randomly selected from the 7 populations) using 30 probe-enzyme combinations revealed a high level of interpopulation polymorphism (62.2%) indicating interpopulation genetic divergence. The polymorphic RAPDs and RFLPs were pooled, and clustering was carried out based on mean similarity for individual populations. The dendrogram showed groupings of populations from the West and East Coasts of India into separate clusters, at 60% similarity level. Further, RAPD and RFLP analysis of male and female plants showed approximately the same level of variation in both sexes, and no sex-linked markers were found. These results demonstrate that considerable intrapopulation and interpopulation genetic variations exist in E. agallocha, and that lack of genetic variation is not the reason for the morphological uniformity observed across the range of the species. Key words: mangroves, Excoecaria agallocha, molecular markers, RAPD, RFLP, genetic variation.

Population genetic variation, differentiation and bottlenecks of Dacrydium pectinatum (Podocarpaceae) in Hainan Island, China: implications for its conservation

2010 ◽  
Vol 58 (4) ◽  
pp. 318 ◽  
Author(s):  
Ying-Juan Su ◽  
Ting Wang ◽  
Feng Deng
Keyword(s):  
Genetic Variation ◽  
Population Genetic ◽  
Management Practices ◽  
Hainan Island ◽  
Issr Markers ◽  
Mode Shift ◽  
Island Populations ◽  
Wide Scale ◽  
Population Genetic Variation ◽  
High Level

Island populations provide an ideal natural experiment with which to study the forces driving population evolution. Seventeen populations of Dacrydium pectinatum de Laubenfels on Hainan Island, China, were sampled throughout its distribution range and then assessed using inter-simple sequence repeats (ISSR) markers. Population genetic parameters were estimated by Bayesian approaches as well as conventional methods. Genetic bottleneck signatures were further dissected by performing three heterozygosity excess tests and the mode-shift indicator test. Compared with other coniferous species, a relatively high level of genetic variation and a low degree of differentiation was revealed in D. pectinatum. In addition, severe bottlenecks were identified at local, regional as well as range-wide scale. Ecological and life-history traits were suggested to play major roles in the shaping of the genetic variation pattern. In particular, long life span could have exerted a lagging effect on both the genetic variation and differentiation of extant populations. Our findings may contribute to improving management practices for the restoration of D. pectinatum.

scholarly journals ISSR-PCR analysis of genetic variation in populations of Achillea L. species in southern part of Krasnoyarsk Territory and Republic of Khakassia

2020 ◽  
Vol 19 (2) ◽  
pp. 090-093
Author(s):  
E. E. Stoyanova ◽  
I. E. Yamskikh
Keyword(s):  
Genetic Variation ◽  
Genetic Variability ◽  
Pcr Analysis ◽  
Minor Variation ◽  
The Republic ◽  
Satisfactory State ◽  
High Level ◽  
A Minor ◽  
Issr Pcr ◽  
Intrapopulation Diversity

ISSR-PCR analysis of populations of two species of the genus Achillea, growing in the southern part ofthe Krasnoyarsk Territory and the Republic of Khakassia, was carried out. The results of the study showed that a highlevel of intrapopulation variability is characteristic of Achillea asiatica and A. millefolium. Relatively low indicators ofgenetic variability are noted for populations Aa3 (A. asiatica) and Ak (A. kuprijanovii). The gene pool of all the studiedpopulations is characterized by a satisfactory state and can independently reproduce. Most of the genetic variability ofAchillea millefolium, A. asiatica, and A. kuprijanovii (74.7 %) accounts for the share of intrapopulation diversity, and25.3 % is a minor variation. The studied populations are characterized by a high level of differentiation and are dividedaccording to a systematic criterion.

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