scholarly journals Histometric evaluation of meat products – determination of area and comparison of results obtained by histology and chemistry

2011 ◽  
Vol 21 (No. 3) ◽  
pp. 101-106 ◽  
Author(s):  
B. Tremlová ◽  
P. Štarha
Keyword(s):  
Histological Examination ◽  
Meat Products ◽  
Absorption Spectrometry ◽  
Poultry Meat ◽  
Alizarin Red ◽  
Image Recording ◽  
Chemical Assay ◽  
Bone Fragments ◽  
The Individual

Histological examination of meat products enables direct identification and differentiation of the individual components. Image analysis is described in literature as a method that gives objective and accurate results comparable with chemical assay findings. The aim of this study was, therefore, to establish a procedure for the quantitative evaluation of meat products based on histological examination. For this reason, bone fragments in poultry meat products were detected. The technique used included the preparation of the mounts (staining with alizarin red), the application of digital photography, processing and analysis of the micrographs (ACC program, Image Structure and Object Analyser, version 4.0). The results were confirmed by comparison with chemical analyses determining the calcium levels by atomic absorption spectrometry. The correlation of both methods can be expressed by the coefficient of 0.78. A modification of the procedure of image recording and analysis was necessary to achieve this result.  

scholarly journals Histometric evaluation of meat products – determination of size and number of objects

2011 ◽  
Vol 20 (No. 5) ◽  
pp. 175-180 ◽  
Author(s):  
B. Tremlová ◽  
P. Štarha
Keyword(s):  
Image Analysis ◽  
Meat Products ◽  
Poultry Products ◽  
Histologic Evaluation ◽  
Bone Fragments ◽  
Bone Particles

In the framewort of the quantitative histologic evaluation of poultry products, the size and the number of bone fragments have been determined using the image analysis. Bone fragments were identified by their colour and analysed automatically. The samples contained 135 to 2167 bone particles the length of which varied from 5 to 2088 μm. Comparing products of the same kind, we found differences in the contents of bone fragments; this fact was possibly due to inadequate observance of the technological procedure by some producers.    

scholarly journals Validation of the method for determination of lead, cadmium, arsene in meat and meat products atomic-absorption spectrometry with electrothermal atom

2021 ◽  
Author(s):  
S. V. Shulyak ◽  
◽  
K. S. Myagka ◽  
S. A. Tkachuk ◽  
O. S. Gaiduy ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Meat Products ◽  
Absorption Spectrometry ◽  
The Body ◽  
Atomic Absorption Spectrophotometer ◽  
Electrothermal Atomization ◽  
Validation Data

The toxicity of heavy metals poses risks to the health of both humans and animals based on their ability to accumulate in the body. The toxicity of the metal depends on the absorbed dose, route and duration of exposure. The timely determination of heavy metals in food, in accordance with EU-approved assessment methods, is an urgent task. The aim of the study was to validate the method of determination of lead, cadmium, arsenic in meat and meat products by atomic absorption spectrometry with electrothermal atomization. The Thermo Solaar atomic absorption spectrophotometer (USA) was used to determine arsenic and cadmium, and the Varian 240 G atomic absorption spectrophotometer (Australia) was used to determine lead. Nitric acid (Merk, Germany), certified standard solutions (Merk, Germany) according to the approved methods were used for mineralization of samples and preparation of background, calibration solutions. The validation characteristics of the method for determining lead, cadmium, arsenic by atomic absorption spectrometry with electrothermal atomization, namely: detection limit, quantitative limit, accuracy, correctness, convergence, reproducibility, selectivity, linearity. The accuracy, practicality and versatility of this method have been proven, which is confirmed by LOD and LOQ: for lead 0.31, 10.0 μg / kg, cadmium 0.3 and 5.0 μg / kg, arsenic 0.2 and 10.0 μg / kg. The results obtained and the validation data on accuracy and reproducibility are in line with the requirements of European legislation on the residual content of heavy metals in meat and meat products.

Detection of some Meat Adulterants in a Raw Minced Meat Product Model using Frozen Tissue Microarray Technique

2020 ◽  
Keyword(s):  
Histological Examination ◽  
Tissue Microarray ◽  
Meat Product ◽  
Meat Products ◽  
Poultry Meat ◽  
Product Model ◽  
Quality Control Laboratory ◽  
Frozen Tissue ◽  
Short Time ◽  
Microarray Technique

In recent years, interest in meat authenticity has been increased. Therefore, twelve groups of beef kofta product were formulated as soya bean, skin, skin emulsion and mechanically deboned poultry meat (MDPM); each of these substances was added with three different concentrations (40, 60 and 80%) in addition to control one. All group samples were subjected to two types of histological examination, conventional paraffin embedding method and frozen tissue microarray. The results of histological examination revealed that both techniques were accurate methods for identification of the separate components of the formulated product and considered extremely useful tools to judge its quality. Moreover, frozen tissue microarray was very helpful in obtaining the result in a short time and allows for examination of a large number of samples at the same time comparing to the conventional method. In addition, no hazard materials like formalin is used, also no ethyl alcohol or xylene is required in this technique. Therefore, microarray technique is reliable and practicable technique that could be used in food quality control laboratory to assess the quality and safety of meat products.

scholarly journals Study of the Versatility of a Graphite Furnace Atomic Absorption Spectrometric Method for the Determination of Cadmium in the Environmental Field

2002 ◽  
Vol 85 (1) ◽  
pp. 219-224 ◽  
Author(s):  
M Isabel Rucandio ◽  
M Dolores Petit-Domínguez
Keyword(s):  
Atomic Absorption ◽  
Graphite Furnace ◽  
Absorption Spectrometry ◽  
Spectrometric Method ◽  
Clear Trend ◽  
Wide Range ◽  
Graphite Furnace Atomic Absorption ◽  
The Individual ◽  
Certified Values

Abstract Cadmium is a representative example of trace elements that are insidious and widespread health hazards. In contemporary environmental analysis, there is a clear trend toward its determination over a wide range of concentrations in complex matrixes. This paper describes a versatile method for the determination of Cd at various levels (0.1–500 μg/g) in several sample types, such as soils, sediments, coals, ashes, sewage sludges, animal tissues, and plants, by graphite furnace atomic absorption spectrometry with Zeeman background correction. The effect of the individual presence of about 50 elements, with an interference/analyte concentration ratio of up to 105, was tested; recoveries of Cd ranged from 93 to 106%. The influence of different media, such as HNO3, HCl, HF, H2SO4, HClO4, acetic acid, hydroxylammonium chloride, and ammonium acetate, in several concentrations, was also tested. From these studies it can be concluded that the analytical procedure is scarcely matrix dependent, and the results obtained for a wide diversity of reference materials are in good agreement with the certified values.

COMPARISON OF VARIOUS METHODS FOR THE CHEMICAL ASSAY OF CORTICOIDS IN URINE

1955 ◽  
Vol 18 (4) ◽  
pp. 374-378
Author(s):  
Mogens Sprechler
Keyword(s):  
Calibration Curve ◽  
Periodic Acid ◽  
Reducing Power ◽  
Standard Technique ◽  
Phosphomolybdic Acid ◽  
Florisil Column ◽  
Chemical Assay ◽  
Chromatographic Procedure ◽  
Acid Reagent

SUMMARY Since 1949 about 10,000 urinary corticoid analyses have been performed routinely in our laboratory. The method used for this purpose was described in 1950 (Sprechler). We determine the corticoids which can be extracted from the urine with chloroform immediately after acidification to pH 1. The extract is washed with sodium hydroxide and water, a Girard separation is performed, and finally the reducing power of the ketonic fraction is measured by means of the phosphomolybdic acid reagent reaction. During the last few years two other chemical reactions have been used for comparison: The formaldehyde and the Porter-Silber method. After a thorough examination of the above methods a standard technique was followed. In the formaldehyde method a microdiffusion in a Conway unit was used instead of distillation of the formaldehyde following the oxidation with periodic acid. The calibration curve was corrected for loss of material by taking the standard doses of DOC through all the procedures of the method. A micromodification of the Porter-Silber method was chosen. Furthermore attempts were made to determine how specific the chromatographic procedure is in the determination of steroids in urinary extracts. For this purpose the Florisil column was used, and the technique described by Nelson & Samuels was followed. Finally we have investigated the glucuronide-bound corticoids in urine in a smaller series of objects.

ПСИХОЛОГІЧНА ДЕТЕРМІНАЦІЯ ДОМІНУЮЧОГО ІНСТИНКТУ У КОНСТРУКТИВНІЙ САМОРЕАЛІЗАЦІЇ ЖІНОК

1970 ◽  
Vol 6 (1) ◽  
pp. 184-193
Author(s):  
Ольга Віговська
Keyword(s):  
Human Life ◽  
Healthy Children ◽  
Self Disclosure ◽  
Self Identity ◽  
Social Nature ◽  
Self Actualization ◽  
Rational Organization ◽  
The Individual ◽  
Age Range

У статті теоретично обґрунтовано феномен конструктивного самозбереження особистості як ознаки самоактуалізації, розкриття власного потенціалу і побудови перспективи розвитку особистості та емпірично виявлено ознаки психологічної детермінації домінуючого інстинкту у конструктивній самореалізації жінок з різним соціальним статусом. Зазначено, що проблема самозбереження асоціюється з особливостями прояву інстинкту самозбереження людини, але потреби вищого порядку зумовлюють соціальну природу її поведінки, яка локалізована у найвищій точці самореалізації. Теоретично обгрунтовано, що самореалізація визначає тенденцію раціональної організації життя людини та проявляється у її почутті задоволеністю життям. З’ясовано, що психологічну основу конструктивного самозбереження становлять індивідуально-типологічні характеристики людини, які відображають психофізіологічні та психосоціальні резерви самореалізації особистості. Розроблена програма емпіричного дослідження, а також комплекс використаних методів математичної обробки результатів дослідження дає змогу конкретизувати психологічний зміст детермінації домінуючого інстинкту у конструктивній самореалізації жінок вікового діапазону 35-45 років та з різним соціальним статусом. У жінок, які виховують проблемну (хвору) дитину, домінує інстинкт "егофільного типу", що виражається у їх надмірному егоцентризмі і супроводжується низькими показниками самоактуалізації, на відміну від досліджуваних жінок, які виховують здорових дітей і у яких на фоні вираженої тенденції до самоактуалізації домінує базовий інстинкт "дослідницького типу" та "лібертофільного типу". This article theoretically proves constructive phenomenon of self identity as signs of self-disclosure own potential and prospects of development of individual construction. In addition, it empirically showes signs of psychological determination of the dominant instinct in a constructive self-determination of women with different social statuses. It was noted that the issue of self-preservation is associated with the peculiarities of manifestation of self-preservation instinct of man, but it needs higher-order cause social nature of the behavior that is localized at the highest point of self-realization. It theorized that self-realization determines the trend of rational organization of human life and manifests itself in its sense of life satisfaction. It was found that the psychological basis of constructive self-preservation of the individual make individually-typological characteristics of a person that reflect physiological and psychosocial reserves of self-realization. The developed program of empirical research, as well as the methods used complex mathematical processing of results of research allows to specify the content of the psychological determination of the dominant instinct of constructive self-realization а women age range of 35-45 years and with different social status. Women who bring up the problem child dominates the instinct of self-preservation, which is reflected in their excessive self-centeredness, and is accompanied by low levels of self-actualization, as opposed to the study of women who are raising healthy children and that against the backdrop of a pronounced tendency to self-actualization, dominated by basic instinct "research type" and "independent type."

Determination of allostatic load dynamics in the assessment of adaptation in temporary workers in the Arctic

2020 ◽  
pp. 547-548
Author(s):  
O. Yu. Atkov ◽  
S. G. Gorokhova
Keyword(s):  
Body Mass Index ◽  
Glucose Level ◽  
Shift Work ◽  
Allostatic Load ◽  
The Arctic ◽  
Temporary Workers ◽  
Short Term ◽  
Short Term Adaptation ◽  
The Individual

The individual dynamics of the allostatic load index was revealed mainly due to changes in the glucose level, body mass index, which makes it applicable for assessing the short-term adaptation to the stay in the conditions of shift work

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