scholarly journals Pattern of fibre type distribution within muscle fascicles of pigs (Sus scrofa domestica)

2008 ◽  
Vol 52 (No. 4) ◽  
pp. 103-109 ◽  
Author(s):  
D. Škorjanc ◽  
A. Šalehar ◽  
I. Eržen ◽  
M. Kastelic

In order to determine breed differences in the fascicle structure of skeletal muscles, longissimus dorsi muscles (LD) and semimembranous muscles (SM), Duroc (D), Large White (LW), German Landrace (GL) and Swedish Landrace (SL) boars were analyzed. Fibre type delineation was based on the method of myofibrillar ATPase staining. The fibres in a fascicle were divided into layers according to their relation to the perimysium. In each layer, the proportions of fibre types I, IIA and IIB were determined and normalized according to the fibre type proportion in the whole fascicle. Generally, a high proportion of IIB fibres on the periphery, a prevalence of type I and IIA in the layer below, and altered proportions of type I, IIA and IIB in layer 3 were found to be the main characteristics of the distribution of fibres in a porcine fascicle. Breed dissimilarity was established in the arrangement of fibre types, particularly in the first and third layer. The breeds that showed the highest proportions of type I and IIB fibres in LD and SM muscles were GL and SL boars.

1996 ◽  
Vol 8 (3) ◽  
pp. 391 ◽  
Author(s):  
MD Fratacci ◽  
M Levame ◽  
A Rauss ◽  
H Bousbaa ◽  
G Atlan

The changes occurring in the histochemical characteristics of the rat diaphragm during the postnatal period were examined. Fibre-type distribution, fibre oxidative capacity, i.e. succinate-dehydrogenase (SDH) activity, and cross-sectional area were compared in the costal (COS) and crural (CRU) regions, and across their abdominal and thoracic surfaces. The proportions of type I and IIb fibres in both COS and CRU increased with age, while the proportion of type IIa fibres progressively decreased. For COS, fibre distribution was homogeneous over the entire muscle and did not change after 4 weeks. For CRU, it was heterogeneous with a higher proportion of type I fibres on the thoracic surface as from the first week. All fibre types significantly increased in cross-sectional area between 1 and 8 weeks, with no significant differences in COS and CRU. Mean SDH activity did not differ between COS and CRU or across the muscles. Mean SDH activities-were low and identical in all fibre types at birth, and then increased, peaking at the 6th week in type I and IIa fibres. When total muscle fibre oxidative capacity was calculated from an index including fibre-type proportion, cross-sectional area and mean SDH activity, it was significantly higher at 1 than at 8 weeks after birth; this might have functional implications for the newborn.


Author(s):  
Orlando Marques da Costa ◽  
João Gilberto Lopes Pereira ◽  
Celso Alves Rodrigues

Estudou-se, em 30 pares de rins de suínos da raça Large White, 15 machos e 15 fêmeas, com aproximadamente quatro meses de idade, procedentes do Matadouro e Frigorífico "Eder", em Itapecerica da Serra, Estado de São Paulo, a distribuição arterial e venosa do hilo deste órgão, mediante disseccão dos elementos vasculares do pedículo renal, após a fixação do material em solução aquosa de formol a 10,0%. Nestes animais, a artéria renal direita fornece de seis (10,0%) a vinte (3,3%) ramos, com maior frequência de dez (20,0%) e a esquerda de quatro (3,3%) a dezoito (3,3%), com maior incidência de dez(16,7%) e demonstrou uma maior concentração no quadrante craniodorsal, seguido dos quadrantes cranioventral, caudoventral e caudodorsal. No que tange ao número de raízes venosas, a veia renal direita apresenta uma variação de uma (10,0%) a cinco (33,8%) raízes, com maior frequência de cinco (33,8%),a esquerda de duas (13,3%) e sete (3,3%), com maior concentração de três e quatro (53,4%), situadas com maior incidência no quadrante cranioventral, seguido dos quadrantes caudoventral, craniodorsal ecaudodorsal. Quanto à situação global, os ramos das artérias renais direita e esquerda se apresentam em maior número de vezes preponderantemente periféricos(43,3%), enquanto as raízes venosas se mostram exclusivamente periféricas (16,7%). Há igualdade no número de ramos das artérias renais e raízes das veias renais, direitas e esquerdas, apenas 1 vez (3,3%), com distribuião desigual nos quadrantes. Não existem diferenças estatisticamente significantes quanto ao sexo. Estudou-se, em 30 pares de rins de suínos da raça Large White, 15 machos e 15 fêmeas, com aproximadamente quatro meses de idade, procedentes do Matadouro e Frigorífico "Eder", em Itapecerica da Serra, Estado de São Paulo, a distribuição arterial e venosa do hilo deste órgão, mediante dissecção do selementos vasculares do pedículo renal, após a fixação do material em solução aquosa de formol a 10,0%.Nestes animais, a artéria renal direita fornece de seis (10,0%) a vinte (3,3%) ramos, com maior frequência de dez (20,0%) e a esquerda de quatro(3,3%) a dezoito (3,3%), com maior incidência de dez(16,7%) e demonstrou uma maior concentração no quadrante craniodorsal, seguido dos quadrantes cranioventral, caudoventral e caudodorsal. No que tange ao número de raízes venosas, a veia renal direita apresenta uma variação o de uma (10,0%) a cinco (33,8%) raízes, com maior frequência de cinco (33,8%),a esquerda de duas (13,3%) e sete (3,3%), com maior concentração de três e quatro (53,4%), situadas com maior incidência no quadrante cranioventral, seguidodos quadrantes caudoventral, craniodorsal e caudodorsal. Quanto à situação global, os ramos das artérias renais direita e esquerda se apresentam em maior número de vezes preponderantemente periféricos(43,3%), enquanto as raízes venosas se mostram exclusivamente periféricas (16,7%). Há igualdade no número de ramos das artérias renais e raízes das veias renais, direitas e esquerdas, apenas 1 vez (3,3%), com distribuição desigual nos quadrantes. Não existem diferenças estatisticamente significantes quanto ao sexo.


2020 ◽  
Vol 65 (No. 6) ◽  
pp. 193-204
Author(s):  
Nicole Lebedová ◽  
Tersia Needham ◽  
Jaroslav Čítek ◽  
Monika Okrouhlá ◽  
Kateřina Zadinová ◽  
...  

This study compared two histochemical staining methods of muscle fibres and evaluated their relationship with the meat quality traits of two high-value porcine muscles. Immunohistochemical (IHC) and adenosine triphosphatase (ATPase) staining was used to assess the cross-sectional area and proportion of fibre-types I, IIa, IIx and IIb in the samples of longissimus lumborum (LL) and psoas major (PM) muscles collected one-hour post-mortem from 25 crossbred pigs [Large White<sub>Sire</sub> × (Landrace × Large White<sub>Dam</sub>)] at an average age of 152 days. Muscles differed in all fibre parameters, except the proportion and relative area of type IIx fibres. The LL muscle exhibited greater fibre cross-sectional areas of all fibre types, higher proportions of type IIb/IIB, and lower proportions of I and IIa fibres than the PM muscle in both staining techniques. These two muscles also differed marginally in moisture, crude protein and intramuscular fat content. The PM muscle showed a low correlation between fibre types and chemical composition, but the LL muscle showed moderate correlations between fibre CSA and area composition for moisture and ash content. After IHC staining, an increase in LL eye muscle area and drip loss were correlated with lower proportions of type I fibres, while a greater proportion of type IIx fibres resulted in increased LL eye muscle area and moisture content. Furthermore, a higher CSA of all fibre types in the LL decreased redness (a*) and moisture content of the muscle. Results showed that IHC is more appropriate than ATPase staining for the assessment of relationships between muscle fibre parameters and meat quality traits in pigs.


1992 ◽  
Vol 32 (3) ◽  
pp. 257-263 ◽  
Author(s):  
B. Kłosowski ◽  
K. Bidwell-Porebska ◽  
D. Kłosowska ◽  
J. Piotrowski

2001 ◽  
Vol 2001 ◽  
pp. 79-79
Author(s):  
M. Christensen ◽  
P. Henckel ◽  
P.P. Purslow

Proteolytic degradation is known to be faster in white muscles than in red muscles (Whipple & Koohmaraie, 1992). Variation in eating quality between muscles has often been correlated to their metabolic properties, as determined by the fibre type distribution. Correlations between fibre type distribution and postmortem proteolysis could result from two possible effects: (1) Due to their inherent differences in metabolic potential, composition and content of proteolytic enzymes, fibres of some types may degrade more than others. (2) The balance of fibre types controls postmortem (p.m.) metabolic characteristics of the muscle as a whole, with all fibre types within it being equally affected. An experiment was conducted to compare the rate of postmortem proteolysis in five porcine muscles differing in fibre type distribution and to compare the rate of proteolysis in type II fibres isolated from these muscles.


1974 ◽  
Vol 22 (3) ◽  
pp. 629 ◽  
Author(s):  
RK Bamber

The presence of two different types of fibres in the wood of the subfamily Phyllanthoideae (Euphorbiaceae) is recorded: type I in which the fibres have moderately thick walls, a clearly defined lumen cavity and a normal birefringence pattern, and type I1 in which the walls are very thick, have no lumen cavity and a birefringence pattern suggesting an absence of an S3 layer. The genera fall into three groups: those containing type I only, those with type II only, and those with both type I and type II. The fibre type in this family appears to be of taxonomic significance.


1998 ◽  
Vol 76 (11) ◽  
pp. 1983-1992 ◽  
Author(s):  
John W Hermanson ◽  
James M Ryan ◽  
Matthew A Cobb ◽  
Jacqueline Bentley ◽  
William A Schutts, Jr.

We identify a novel histochemical fibre type that is correlated with a unique myosin heavy chain isoform in the pectoralis muscle of the two bats Artibeus lituratus and Carollia perspicillata (Phyllostomidae). Pectoralis muscles sampled from four other species (Phyllostomus hastatus, Platyrrhinus helleri, Glossophaga soricina, and Diaemus youngi) exhibited myosin heavy chain isoforms with electrophoretic properties identical with those of A. lituratus andC. perspicillata. The pectoralis muscles of A. lituratus and C. perspicillata consisted mainly of the newly described type IIe fibre. Diaemus youngi differed from the other species studied by having an FM5 native myosin isoform, a feature shared with Desmodus rotundus. We hypothesize that the two-fibre-type composition of the pectoralis muscle in the phyllostomid bats may be related to a "two-gear" locomotor strategy and represents a synapomorphy. The distribution of fibre types and myosin isoforms correlated with family-level phylogenetic affinity rather than with functional characteristics. One of the fibre types was identical with type IIa of terrestrial mammals and a correlated IIa isoform pattern upon electrophoretic analysis. The IIa fibres had high oxidative and glycolytic potential. The second fibre type, which we call type IIe, had a histochemical and immunocytochemical pattern inconsistent with interpretation as a type IIb muscle fibre. Electrophoretic analysis confirmed that isoforms correlated with IIe fibres migrated at different rates from type I, IIa, IIb, and IIx isoforms. The IIe fibres had a fast oxidative metabolic pattern and were at least twice as numerous as IIa fibres. Thus, a novel type IIe fibre is predominant in the pectoralis of six phyllostomid species that exhibit highly divergent body sizes and (or) flight styles.


2002 ◽  
Vol 2002 ◽  
pp. 175-175 ◽  
Author(s):  
A. Q. Sazili ◽  
T. Parr ◽  
P. L. Sensky ◽  
S.W. Jones ◽  
R.G. Bardsley ◽  
...  

The characterisation of muscle fibres has become increasingly important as the proportion of slow and fast fibre types are known to influence the biochemical and physiological properties of muscle during postmortem tenderisation (Ouali and Talmant, 1990). Current histochemical methods are labour intensive, time consuming and hazardous, requiring rapid freezing of samples in isopentane cooled in liquid nitrogen. The purpose of this study was to investigate an alternative immunochemical approach for identifying fibre types by examining the expression of slow myosin heavy chain (MHC-s) and fast myosin heavy chain (MHC-f) and comparing the data with classical histochemical techniques. Five different ovine skeletal muscles with known differences in fibre types distribution were studied.


2014 ◽  
Vol 14 (4) ◽  
pp. 955-966 ◽  
Author(s):  
Anna Bereta ◽  
Mirosław Tyra ◽  
Katarzyna Ropka-Molik ◽  
Dorota Wojtysiak ◽  
Marian Różycki ◽  
...  

Abstract The objective of this study was to analyse differences in individual fibre types in the histological profile of the longissimus dorsi muscle and their effect on pork carcass lean content and level of intramuscular fat (IMF) content, which determines palatability of meat and meat products. Analysis showed that the amount of type IIB fibres had a statistically significant (P<0.05) effect on the IMF content of the longissimus dorsi muscle. Animals with more than 70% of type IIB fibres in this muscle were also characterized by larger loin eye area (P<0.01) and loin eye height (P<0.05). Analogous relationships were noted when the analysed group of animals was divided according to the diameter of type IIA fibres. IMF was negatively correlated to the percentage of type IIB fibres (rP= -0.162). Relationships with the other two fibre types were positive (IIA - rP= 0.097; I - rP= 0.187). It was found that increased percentage of type IIB fibres resulted in a slightly greater loin weight (rP= 0.176), higher loin eye height (rP= 0.136), larger loin eye area (rP= 0.265) and higher carcass lean content (rP= 0.204). Likewise, the increase in the number of type IIA and type I fibres decreased these parameters.


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