scholarly journals Production of callus and roots from lateral meristems of Loeselia mexicana

2018 ◽  
Vol 96 (3) ◽  
pp. 405
Author(s):  
Lizeth Aguirre-Alberto ◽  
Maria De Lourdes Martinez-Cardenas
Keyword(s):  
Secondary Forest ◽  
Structural Development ◽  
Shoot Induction ◽  
Wild Populations ◽  
Callus Production ◽  
Green Callus ◽  
Collection Methods ◽  
Distal Stem ◽  
Stem Segments

<p><strong>Background</strong>: <em>Loeselia mexicana</em>, known as Mexican false calico, or <em>espinosilla</em> in Spanish, is a widely distributed secondary forest plant with a significant number of medicinal and cosmetic uses. This species is threatened by the lack of regulation over collection methods and changes in land use. <em>In vitro</em> culture could be used to preserve the species by shoot induction, callus production and cell-suspension to obtain secondary metabolites; this would reduce the need to affect wild populations.</p><p><strong>Hypothesis</strong>: A combination of cytokinins and auxins can induce structural development in the plant, promoting the formation of shoots, roots or callus <em>in vitro</em>. By applying this combination to <em>L. mexicana</em> stem segments, we expected to observe new shoots or callus.</p><p><strong>Study site and dates</strong>: “El Teuhtli” volcano, Xochimilco; from June 2015 to February 2016.</p><p><strong>Methods</strong>: Distal stems cuttings were used as explants. They were disinfected with 1 % soap, 0.6 sodium hypochlorite and 70 % ethanol, and rinsed with distilled water. Two different times of disinfection with ethanol were tested. The distal stem segments were then planted in solid MS medium with 3, 5 or 7 mg L<sup>-1</sup> KIN combined with 3 mg L<sup>-1</sup> NAA, and 2 % AC.</p><p><strong>Results</strong>: A favorable response was observed in the treatment with 5 mg L<sup>-1</sup> KIN and 3 mg L<sup>-1</sup> NAA, which produced green callus with root in five weeks. Furthermore, a lower explant mortality rate was achieved, 46.66 % in three weeks, with a shorter disinfection time.</p><strong>Conclusions</strong>: Disinfection time is important for this species, and callus production is possible.

scholarly journals Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro

2012 ◽  
Vol 40 (1) ◽  
pp. 107 ◽  
Author(s):  
Cuiqin YANG ◽  
Yaoguo QIN ◽  
Xin SUN ◽  
Shu YUAN ◽  
Honghui LIN
Keyword(s):  
Shoot Regeneration ◽  
Culture Medium ◽  
Shoot Proliferation ◽  
Shoot Formation ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Induction ◽  
Leaf Base ◽  
Stem Segments

An efficient protocol was established for Sedum spectabile Boreau propagation. Various leaf parts were used as explants to regenerate plantlets, the stem segments of which were cultured for shoot proliferation and plantlet multiplication. The results showed that the leaf base was the optimal explant, as compared to both the middle and the top of leaves, for shoot formation. The highest shoot induction of 88.9% was observed on MS medium supplemented with 0.6 mg/l TDZ and 0.1 mg/l NAA. Hyperhydric leaves obtained in primary culture developed first into abnormal somatic embryos 10 days after subculture, and then into hyperhydric plantlets after an additional 10 days. The hyperhydric plantlets reversed to normal plantlets when plant growth regulators were removed from culture medium. Further, stem segments from reversed plantlets were used for shoot regeneration and root induction. Optimal shoot regeneration was obtained in MS medium containing 0.6 mg/l TDZ with 0.1 mg/l NAA. Root induction and root mean number were all higher on auxin-free medium than on medium containing auxins.

Development of an Agrobacterium-mediated Transformation System for `Beurre Bosc' Pear

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 461d-461
Author(s):  
Richard L. Bell ◽  
Ralph Scorza ◽  
Chinnathambi Srinivasan
Keyword(s):  
Shoot Proliferation ◽  
Induction Medium ◽  
Shoot Induction ◽  
Transformation System ◽  
Gus Histochemical Assay ◽  
Young Leaves ◽  
Leaf Tissues ◽  
Shoot Induction Medium ◽  
Basal Salts

An efficient regeneration/transformation system was developed for `Beurre Bosc' pear. Young leaves were harvested from in vitro shoots proliferated on a medium containing MS basal salts and 5 BAP, 0.5 μM IBA, and 0.6M3. Shoot regeneration was optimized using a modification of the medium of Chevreau and Leblay (1993). Explants were cultured on shoot induction medium contained 10 μM TDZ and 1 μM IBA for 4 weeks in the dark, and then transfered to a similar, but auxinless, regeneration medium until shoots developed, usually after an additional 4 to 8 weeks. Leaf tissues were transformed by co-cultivation for 3 days with Agrobacterium tumefaciens EHA101 carrying a pGA482 plasmid containing NPTII, GUS, and rolC genes, followed by cultivation on SIM containing 300 mg/L timentin. Putative transgenic plants were selected on shoot induction medium containing 80mg/L kanamycin, and multiplied on shoot proliferation medium. Four clones were confirmed as transgenic using the GUS histochemical assay and Southern blots for the NPTII and rolC genes. Plants of each clone have been rooted and successfully transfered to the greenhouse for further analysis of gene expression.

scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

The induction of flowering in vitro in stem segments of Plumbago indica L.

Planta ◽  
1967 ◽  
Vol 72 (4) ◽  
pp. 355-370 ◽  
Author(s):  
Colette Nitsch ◽  
J. P. Nitsch
Keyword(s):  
Plumbago Indica ◽  
Flowering In Vitro ◽  
Stem Segments

An In-Vitro Investigation into the Cement Pressurization Achieved During Insertion of Four Different Femoral Stems

2005 ◽  
Vol 219 (6) ◽  
pp. 407-413 ◽  
Author(s):  
C Gozzard ◽  
S Gheduzzi ◽  
A W Miles ◽  
I D Learmonth
Keyword(s):  
Revision Surgery ◽  
In Vitro Study ◽  
The Other ◽  
Stem Volume ◽  
Factors Associated ◽  
In Vitro Investigation ◽  
Femoral Stems ◽  
Stem Shape ◽  
Distal Stem

Adequate cement pressurization during stem insertion improves the interdigitation of cement into bone. This increases the strength of the cement-bone interface, thus contributing to the reduction of the incidence of aseptic loosening, the commonest cause of revision surgery. This in-vitro study compared the cement pressurization achieved during insertion of four different stems of equivalent sizes: the Elite Plus (DePuy, UK), C-Stem (DePuy, UK), Exeter (Stryker, USA), and CPS-Plus (Plus Orthopedics, Switzerland). The maximum pressures attained at the time of stem insertion were recorded at proximal, mid and distal stem levels. The Elite Plus generated significantly higher distal pressures than the other stems. The CPS-Plus generated significantly greater proximal cement pressures than the Elite Plus, C-Stem, and Exeter prostheses. The triple taper of the C-Stem increased the cement pressurization medial to the stem. The stem shape and the presence or absence of a proximal stem centralizer affect cement pressurization. The presence of a proximal stem centralizer, a large stem volume, and a lateral-medial taper are all factors associated with increased cement pressurization during stem insertion.

The Development of Intracellular Membranes Concomitant with the Appearance of HCL Secretion in Oxyntic Cells of the Metamorphosing Bullfrog Tadpole

1969 ◽  
Vol 4 (3) ◽  
pp. 709-727
Author(s):  
GERTRUDE M. FORTE ◽  
L. LIMLOMWONGSE ◽  
J. G. FORTE
Keyword(s):  
Endoplasmic Reticulum ◽  
Apical Cell ◽  
Morphological Development ◽  
Structural Development ◽  
Gastric Glands ◽  
Cytoplasmic Matrix ◽  
Stage Of Development ◽  
Hcl Secretion ◽  
Bullfrog Tadpole

Bullfrog tadpole stomachs of various metamorphic stages were examined to determine the fine-structural development of oxyntic cells and to correlate observed morphological development with the capacity to secrete HCl. It was found that in vitro tadpole stomachs can consistently be stimulated to secrete acid by stage XXIV of metamorphosis, when tail reabsorption is nearly complete. Concomitant with the appearance of HCl secretion, identifiable oxyntic cells were found in the gastric glands. Prior to stage XXIV (stages XXI and XXII) the majority of cells present in the developing gastric glands exhibit features of cytological organization characteristic of undifferentiated cells: large nuclei, relatively scantry cytoplasm, and numerous ribosomal particles within the cytoplasmic matrix. The newly differentiated oxyntic cells of stage XXIV tadpole stomachs are recognizable by the accumulation of tubular members of the smooth-surfaced endoplasmic reticulum in the apical portion of the cells. These membranous structures appear to be formed by the Golgi complex which is extremely elaborate at this stage of development. As the animals complete metamorphosis (stage XXV) further development of the oxyntic cells occurs, especially the elaboration of the tubular components of the smooth-surfaced endoplasmic reticulum. The abundance of these membranous tubules within the apical cell regions and the pattern of their packing is similar to that observed in oxyntic cells of adult frogs. Also consistent with studies on adult frogs, structural alterations associated with HCl secretion were seen in the later stages of metamorphosis. In stages XXIV and XXV tadpole stomachs, which had been stimulated to secrete acid by addition of histamine, the apical surfaces of oxyntic cells were invested with long filamentous microvilli which projected into the glandular lumen. These observations support the hypothesis that membrane transformations play an integral role in the mechanism of HCl secretion and they implicate the morphogenesis of the smooth-surfaced endoplasmic reticulum as a basic prerequisite in the development of gastric secretory function.

scholarly journals In vitro Regeneration and Rapid Multiplication of Dendrobium bensoniae, an Indigenous Ornamental Orchid

2017 ◽  
Vol 14 (2) ◽  
pp. 24-31 ◽  
Author(s):  
S S Riva ◽  
A Islam ◽  
M E Hoque
Keyword(s):  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Rapid Multiplication ◽  
Number Of Shoots

An experiment was conducted on in vitro regeneration and multiplication of Dendrobium bensoniae. Different concentrations of BA and IBA alone or combination of both hormones were used as treatment for regeneration.  It was revealed that shoot regeneration from node was the best at 2.0 mg/l BA supplemented to MS medium. It gave better responses than all other concentrations and combinations of BA and BA+IBA, used in the present study. The highest number of shoots and leaves were found when 1.0 mg/l BA with 1.5 mg/l IBA was supplemented into MS medium.  For rooting, 0.5 mg/l BA with 1.0 mg/l IBA was found to be the most effective. The well-rooted plantlets were successfully acclimatized under 70-80% humidity and planted in pots and transferred to the shade house for establishment. Around 85% of plantlets survived in the field. From the present result, it may be recommended that MS medium supplemented with 2.0 mg/l BA may be used for rapid shoot induction and regeneration of D. bensoniae.The Agriculturists 2016; 14(2) 24-31

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