scholarly journals Effects of ethylene inhibitors, silver nitrate (AgNO3), cobalt chloride (CoCl2) and aminooxyacetic acid (AOA), on in vitro shoot induction and rooting of banana (Musa acuminata L.)

2015 ◽  
Vol 14 (32) ◽  
pp. 2510-2516 ◽  
Author(s):  
M Tamimi Samih
Keyword(s):  
Silver Nitrate ◽  
Cobalt Chloride ◽  
Musa Acuminata ◽  
Aminooxyacetic Acid ◽  
Shoot Induction ◽  
Ethylene Inhibitors

scholarly journals Can Ethylene Inhibitors Enhance the Success of Olive Somatic Embryogenesis?

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 168
Author(s):  
Muhammad Ajmal Bashir ◽  
Cristian Silvestri ◽  
Amelia Salimonti ◽  
Eddo Rugini ◽  
Valerio Cristofori ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Salicylic Acid ◽  
Silver Nitrate ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Cobalt Chloride ◽  
Zygotic Embryos ◽  
Ethylene Inhibitors ◽  
Embryogenic Calli ◽  
Stimulatory Action

An efficient in vitro morphogenesis, specifically through somatic embryogenesis, is considered to be a crucial step for the application of modern biotechnological tools for genetic improvement in olive (Olea europaea L.). The effects of different ethylene inhibitors, i.e., cobalt chloride (CoCl2), salicylic acid (SA), and silver nitrate (AgNO3), were reported in the cyclic somatic embryogenesis of olive. Embryogenic callus derived from the olive immature zygotic embryos of the cultivar Leccino, was transferred to the expression ECO medium, supplemented with the ethylene inhibitors at 20 and 40 µM concentrations. Among these, the maximum number of somatic embryos (18.6) was obtained in media containing silver nitrate (40 µM), followed by cobalt chloride (12.2 somatic embryos @ 40 µM) and salicylic acid (40 µM), which produced 8.5 somatic embryos. These compounds interfered on callus traits: white friable embryogenic calli were formed in a medium supplemented with 40 µM cobalt chloride and salicylic acid; in addition, a yellow-compact embryogenic callus appeared at 20 µM of all the tested ethylene inhibitors. The resulting stimulatory action of silver nitrate among all the tested ethylene inhibitors on somatic embryogenesis, clearly demonstrates that our approach can efficiently contribute to the improvement of the current SE protocols for olive.

scholarly journals INDUKSI TUNAS PADA BEBERAPA TIPE PEMOTONGAN EKSPLAN BONGGOL PISANG UDANG (Musa acuminata Colla) SECARA IN VITRO

2020 ◽  
Vol 8 (1) ◽  
pp. 20
Author(s):  
Mayta Novaliza Isda
Keyword(s):  
Block Design ◽  
Morphological Characteristics ◽  
Musa Acuminata ◽  
Shoot Length ◽  
Fruit Colour ◽  
Shoot Induction ◽  
Genome Species ◽  
Randomized Block Design

Musa acuminata Colla is a unique genome species different from other species. This species has morphological characteristics on its reddish-purple fruit colour. Nowadays, Musa acuminata Colla is rarely to fond in Riau especially in Kampar District. This research aims to know the influence of several kinds of corm bananas cutting that comes from Kampar district with in vitro and determined concentration of single BAP and combination of BAP and Kinetin best in forming Musa acuminata Colla shoot. This research used for randomized block design (RBD) by giving BAP concentration (0, 4, 8 mg/l and combination of  BAP and Kinetin (0 mg/l BAP+0,4 mg/l Kinetin, 4 mg/l BAP+0,4 mg/l Kinetin, 8 mg/l BAP+ 0,4 mg/l Kinetin) with different cutting type the whole and cut into two parts on MS media with 5 replications. The result of this research showed the addition of BAP dan combination of BAP and Kinetin is giving the best result on the percentage of live explants and shot formation at 100%. Treatment of 8 mg/l BAP resulted in the highest shoot percentage up to 100%, the fastest shoots appeared at 34.00 days after planting and the highest shoot length of 2.83 cm with the shoots of 2.33 on cutting halved. Keywords : BAP, in vitro, kinetin, Musa acuminata Colla, shoot induction 

scholarly journals Improvement of In Vitro Culturing of Habanero Pepper by Inhibition of Ethylene Effects

HortScience ◽  
2006 ◽  
Vol 41 (2) ◽  
pp. 405-409 ◽  
Author(s):  
Nancy Santana-Buzzy ◽  
Adriana Canto-Flick ◽  
Lourdes G. Iglesias-Andreu ◽  
María del C. Montalvo-Peniche ◽  
Guadalupe López-Puc ◽  
...  
Keyword(s):  
Silver Nitrate ◽  
Ethylene Production ◽  
Culture Medium ◽  
Cobalt Chloride ◽  
Second Phase ◽  
In Vitro Production ◽  
Habanero Pepper ◽  
Negative Effect ◽  
High Concentrations

The in vitro production of ethylene and its effects on the development of Habanero pepper (Capsicum chinense Jacq.) plantlets were evaluated using nonventilated containers (NVCs) and ventilated containers (VCs). Shoots of Habanero pepper between 0.5 and 1.0 cm of height were cultivated in Magenta culture boxes and samples of the headspace atmosphere were taken every four days during the previously established culturing time of 40 days. The presence of ethylene was detected in the NVCs and produced a negative effect on the development of plantlets. In a second phase of this work, the effect of silver nitrate (AgNO3) and cobalt chloride (CoCl2) on ethylene production was evaluated during in vitro development of Habanero pepper plantlets. Concentrations of 50, 300, and 500 μm of each ethylene inhibitor were used in the culture medium. Although cobalt chloride partially inhibited the production of ethylene during in vitro culture of this species, at low concentrations the plantlets presented some degree of vitrification and the highest concentration proved to be toxic for the plantlets. Silver nitrate added to the culture medium did not inhibit ethylene production, however, it did inhibit the effect of this hormone on the plantlets. In fact, when high concentrations of silver nitrate were used (300 μm), high amounts of ethylene were detected in the headspace of the vessels and plantlets were actually healthier.

scholarly journals Optimization of benzyl amino purines (BAP) concentration and medium type on the induction of banana shoots (Musa acuminata Colla.) cv. Barangan Merah under in vitro condition

2022 ◽  
Vol 951 (1) ◽  
pp. 012015
Author(s):  
M Setyowati ◽  
Efendi ◽  
Alfizar ◽  
E Kesumawati
Keyword(s):  
Solid Medium ◽  
Musa Acuminata ◽  
Gram Negative Bacteria ◽  
Shoot Induction ◽  
Gram Positive Bacteria ◽  
The Right ◽  
Solid Liquid ◽  
Medium Type ◽  
Better Than

Abstract The use of optimum concentration of BAP and the right medium type can support shoot induction on the explant of banana sucker cv.Barangan Merah. The Research was conducted at the Laboratory of Tissue Culture, Faculty of Agriculture, Universitas Syiah Kuala. This research used a completely randomized 3x3 factorial design. The first factor was Benzyl Amino Purines (BAP) concentration at three levels of concentration, i.e. control, 3 mg.L-1, and 6 mg.L-1. The second factor was Musrahige & Skoog (MS) medium type at three levels, i.e. solid, solid-liquid, and liquid. Results showed that the BAP treatment of 3 mg.L-1 had the biggest response to the number of open midribs compared to other BAP treatments. The type of solid medium tends to shoot induction better than other types of medium. The contamination that occurred was 13.9% of the 72 explants planted. The contamination was caused by Mucor and Aspergillus fungi. The bacteria causing the contamination were gram positive bacteria (coccus) and gram-negative bacteria (coccus and bacilli).

scholarly journals In Vitro Shoot Induction of Musa acuminata cv. Mas Kirana

2020 ◽  
Vol 13 (1) ◽  
pp. 34
Author(s):  
Raden Roro Rifka Annisa ◽  
Arkan Setiaji ◽  
Aries Bagus Sasongko
Keyword(s):  
Somatic Embryos ◽  
Shoot Formation ◽  
Musa Acuminata ◽  
Ms Medium ◽  
Histological Observation ◽  
Shoot Induction ◽  
Shoot Height ◽  
Mother Plants ◽  
Banana Cultivar

Abstract: The availability of �mother plants� used for source of explants is one of the most important limitations for the development of in vitro culture in new banana cultivar�s. To increase produce large quantities of uniform and healthy seedlings, induction of multiplication has been carried out on Musa acuminata cv. Mas Kirana. Plant regeneration were obtained by culturing sucker on MS medium supplemented with PGRs BAP and NAA (1 mg/l + 0.5 mg/l, 2 mg/l + 0.5 mg/l, and 3 mg/l + 0.5 mg/l) and TDZ (0.25 mg/l, 0.5 mg/l, and 0.75 mg/l). The highest shoot formation was found in 0.25 mg/l TDZ with average shoots of 13.67 � 3.16 and primary shoot height is 20 cm. TDZ induction tends to form somatic embryos, while induction of BAP and NAA leads to organogenesis. The rate of contaminants occurrence from bacteria is 87.50% and 12.50% from fungus. In histological observation, TDZ, BAP, and NAA affect the development of many new meristematic zones seen in the scalps.

FACTORS AFFECTING THE REGENERATION OF PEPPER (CAPSICUM ANNUUM L.)

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1120G-1120
Author(s):  
J. L. Jacobs ◽  
C. T. Stephens
Keyword(s):  
Growth Hormone ◽  
Silver Nitrate ◽  
Callus Formation ◽  
Leaf Explants ◽  
Regeneration System ◽  
Factors Affecting ◽  
Nitrate Concentrations ◽  
Leaf Differentiation ◽  
Whole Plants

Several growth hormone combinations and silver nitrate concentrations were examined for their effect on regeneration of different pepper genotypes. Primary leaf explants from in vitro seedlings were cultured on a revised Murashige and Skoog medium supplemented with auxin, cytokinin and 1.6% glucose. Combinations of different concentrations of indole-3-acetic acid (IAA), 0-5 mg/l, and 6-benzylaminopurine (BAP), 0-5 mg/l, were tested to determine the most effective medium for shoot primordium formation. Experiments with IAA and BAP did not result in a specific growth hormone combination appropriate for regeneration of all genotypes tested. Of the silver nitrate concentrations tested, 10 mg/l resulted in the best shoot and leaf differentiation and reduced callus formation. Differences in organogenic response of individual genotypes were evaluated on a single regeneration medium. Whole plants were regenerated from 11 of 63 genotypes examined. Based on these experiments, a reproducible regeneration system for pepper was developed with a total of 500 plants regenerated to date.

Development of an Agrobacterium-mediated Transformation System for `Beurre Bosc' Pear

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 461d-461
Author(s):  
Richard L. Bell ◽  
Ralph Scorza ◽  
Chinnathambi Srinivasan
Keyword(s):  
Shoot Proliferation ◽  
Induction Medium ◽  
Shoot Induction ◽  
Transformation System ◽  
Gus Histochemical Assay ◽  
Young Leaves ◽  
Leaf Tissues ◽  
Shoot Induction Medium ◽  
Basal Salts

An efficient regeneration/transformation system was developed for `Beurre Bosc' pear. Young leaves were harvested from in vitro shoots proliferated on a medium containing MS basal salts and 5 BAP, 0.5 μM IBA, and 0.6M3. Shoot regeneration was optimized using a modification of the medium of Chevreau and Leblay (1993). Explants were cultured on shoot induction medium contained 10 μM TDZ and 1 μM IBA for 4 weeks in the dark, and then transfered to a similar, but auxinless, regeneration medium until shoots developed, usually after an additional 4 to 8 weeks. Leaf tissues were transformed by co-cultivation for 3 days with Agrobacterium tumefaciens EHA101 carrying a pGA482 plasmid containing NPTII, GUS, and rolC genes, followed by cultivation on SIM containing 300 mg/L timentin. Putative transgenic plants were selected on shoot induction medium containing 80mg/L kanamycin, and multiplied on shoot proliferation medium. Four clones were confirmed as transgenic using the GUS histochemical assay and Southern blots for the NPTII and rolC genes. Plants of each clone have been rooted and successfully transfered to the greenhouse for further analysis of gene expression.

scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

Inhibition of Adenosine Triphosphatases in Vitro by Silver Nitrate and Silver Sulfadiazine

1984 ◽  
Vol 3 (1) ◽  
pp. 37-42 ◽  
Author(s):  
B. R. Nechay ◽  
J. P. Saunders
Keyword(s):  
Silver Nitrate ◽  
Aqueous Suspension ◽  
Human Kidney ◽  
Silver Sulfadiazine ◽  
Constant Concentration ◽  
Inhibitory Potency ◽  
Adenosine Triphosphatases ◽  
Tissue Homogenates ◽  
Atpase Inhibition

Inhibition of adenosine triphosphatase (ATPase) by silver nitrate (AgNO3) in vitro was studied in microsomal fractions or tissue homogenates of canine brain and kidney and human kidney. In microsomal fractions, AgNO3 was an indiscriminate inhibitor of ouabain-sensitive (Na+ + K+ ATPase) and ouabain-insensitive (Mg2+ ATPase) activities, with 50% inhibition obtaining at concentrations on the order of 10–7 to 10–6 M. Changing the concentrations of Na+, K+, H+, Mg2+, and ATP did not alter the fractional inhibition of Na+ + K+ ATPase by a constant concentration of AgNO3. An aqueous suspension of silver sulfadiazine had an inhibitory potency similar to AgNO3. It was concluded that silver gives a different pattern of Na+ + K+ ATPase inhibition than other metallic inhibitors of the enzyme so far examined.

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