The role of neprilysin in ocular surface homeostasis and corneal wound healing

2020 ◽  
Author(s):  
Rachel Marie Genova
Keyword(s):  
Wound Healing ◽  
Ocular Surface ◽  
Corneal Wound Healing ◽  
Corneal Wound

scholarly journals SFTA3 – a novel surfactant protein of the ocular surface and its role in corneal wound healing and tear film surface tension

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Martin Schicht ◽  
Fabian Garreis ◽  
Nadine Hartjen ◽  
Stephanie Beileke ◽  
Christina Jacobi ◽  
...  
Keyword(s):  
Surface Tension ◽  
Wound Healing ◽  
Ocular Surface ◽  
Tear Film ◽  
Film Surface ◽  
Surfactant Protein ◽  
Corneal Wound Healing ◽  
Corneal Wound

scholarly journals The Role of Neutrophils in Corneal Wound Healing in HO-2 Null Mice

PLoS ONE ◽  
2011 ◽  
Vol 6 (6) ◽  
pp. e21180 ◽  
Author(s):  
Giuseppina Marrazzo ◽  
Lars Bellner ◽  
Adna Halilovic ◽  
Giovanni Li Volti ◽  
Filippo Drago ◽  
...  
Keyword(s):  
Wound Healing ◽  
Corneal Wound Healing ◽  
Corneal Wound

MicroRNA Expression Profile and the Role of miR-204 in Corneal Wound Healing

2015 ◽  
Vol 56 (6) ◽  
pp. 3673 ◽  
Author(s):  
Jianhong An ◽  
Xiaoyan Chen ◽  
Weiwei Chen ◽  
Rongxin Liang ◽  
Peter S. Reinach ◽  
...  
Keyword(s):  
Wound Healing ◽  
Expression Profile ◽  
Microrna Expression ◽  
Corneal Wound Healing ◽  
Microrna Expression Profile ◽  
Corneal Wound

scholarly journals Potential role of corneal epithelial cell-derived exosomes in corneal wound healing and neovascularization

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Kyu-Yeon Han ◽  
Jennifer A. Tran ◽  
Jin-Hong Chang ◽  
Dimitri T. Azar ◽  
James D. Zieske
Keyword(s):  
Wound Healing ◽  
Epithelial Cell ◽  
Potential Role ◽  
Corneal Epithelial Cell ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Corneal Wound

scholarly journals Insulin-like Factor 3 Promotes Wound Healing at the Ocular Surface

Endocrinology ◽  
2013 ◽  
Vol 154 (6) ◽  
pp. 2034-2045 ◽  
Author(s):  
Ulrike Hampel ◽  
Thomas Klonisch ◽  
Saadettin Sel ◽  
Ute Schulze ◽  
Friedrich P. Paulsen
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Cell Lines ◽  
Ocular Surface ◽  
Corneal Wound Healing ◽  
Cognate Receptor ◽  
Lacrimal Apparatus ◽  
Corneal Wound ◽  
And Gender ◽  
And Migration

Abstract Tear fluid is known to contain many different hormones with relevance for ocular surface homeostasis. We studied the presence and functional role of insulin-like factor 3 (INSL3) and its cognate receptor RXFP2 (relaxin/insulin-like family peptide receptor 2) at the ocular surface and in tears. Expression of human INSL3 and RXFP2 was determined in tissues of the ocular surface and lacrimal apparatus; in human corneal (HCE), conjunctival (HCjE), and sebaceous (SC) epithelial cell lines; and in human tears by RT-PCR and ELISA. We investigated effects of human recombinant INSL3 (hrINSL3) on cell proliferation and cell migration and the influence of hrINSL3 on the expression of MMP2, -9, and -13 and TIMP1 and -2 was quantified by real-time PCR and ELISA in HCE, HCjE, and SC cells. We used a C57BL/6 mouse corneal defect model to elucidate the effect of topical application of hrINSL3 on corneal wound healing. INSL3 and RXFP2 transcripts and INSL3 protein were detected in all tissues and cell lines investigated. Significantly higher concentrations of INSL3 were detected in tears from male vs. female volunteers. Stimulation of HCE, HCjE, and SC with hrINSL3 significantly increased cell proliferation in HCjE and SC and migration of HCjE. Treatment with hrINSL3 for 24 hours regulated MMP2, TIMP1, and TIMP2 expression. The local application of hrINSL3 onto denuded corneal surface resulted in significantly accelerated corneal wound healing in mice. These findings suggest a novel and gender-specific role for INSL3 and cognate receptor RXFP2 signaling in ocular surface homeostasis and determined a novel role for hrINSL3 in corneal wound healing.

The Role of Extracellular Matrix in Corneal Wound Healing

Cornea ◽  
2013 ◽  
Vol 32 ◽  
pp. S43-S45 ◽  
Author(s):  
Osamu Yamanaka ◽  
Takayoshi Sumioka ◽  
Shizuya Saika
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Corneal Wound Healing ◽  
Corneal Wound

scholarly journals Role of VIP and Sonic Hedgehog signaling pathways in mediating epithelial wound healing, sensory nerve regeneration and their defects in diabetic corneas

2020 ◽  
Author(s):  
Ada Admin ◽  
Yangyang Zhang ◽  
Nan Gao ◽  
Lin Wu ◽  
Patrick S. Y. Lee ◽  
...  
Keyword(s):  
Wound Healing ◽  
Nerve Regeneration ◽  
Sonic Hedgehog ◽  
Sensory Nerve ◽  
Dependent Manner ◽  
Corneal Wound Healing ◽  
Epithelial Wound Healing ◽  
Corneal Wound ◽  
Diabetic Keratopathy

Diabetic Keratopathy, a sight-threatening corneal disease, comprises several symptomatic conditions including delayed epithelial wound healing, recurrent erosions, and sensory nerve (SN) neuropathy. We investigated the role of neuropeptides in mediating corneal wound healing, including epithelial wound closure and SN regeneration. Denervation by Resiniferatoxin severely impaired corneal wound healing and markedly up-regulated pro-inflammatory gene expression. Exogenous neuropeptides CGRP, SP, and VIP partially reversed Resiniferatoxin’s effects, with VIP specifically inducing IL-10 expression. Hence, we focused on VIP and observed that wounding induced VIP and VIPR1 expression in normal (NL), but not diabetic (DM) mouse corneas. Targeting VIPR1 in <em>NL</em> corneas attenuated corneal wound healing, dampened wound-induced expression of neurotrophic factors, and exacerbated inflammatory responses while exogenous VIP had the opposite effects in DM corneas. Remarkably, wounding and diabetes also affected the expression of Sonic Hedgehog (SHH) in a VIP-dependent manner. Downregulating SHH expression in NL corneas decreased, while exogenous SHH in DM corneas increased the rates of corneal wound healing. Furthermore, inhibition of SHH signaling dampened VIP-promoted corneal wound healing. We conclude that VIP regulates epithelial wound he<a></a><a>aling, inflammatory response, and nerve regeneration in the corneas in a</a> SHH-dependent manner, suggesting a therapeutic potential for these molecules in treating diabetic keratopathy.

scholarly journals The role of hepatocyte growth factor in corneal wound healing

2018 ◽  
Vol 166 ◽  
pp. 49-55 ◽  
Author(s):  
Hidetaka Miyagi ◽  
Sara M. Thomasy ◽  
Paul Russell ◽  
Christopher J. Murphy
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Corneal Wound Healing ◽  
Corneal Wound ◽  
Hepatocyte Growth
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