scholarly journals Early degeneration of the epithelial cells in the initial segment of the epididymal duct in mice after efferent duct cutting.

1989 ◽  
Vol 52 (3) ◽  
pp. 299-310 ◽  
Author(s):  
Kazuhiro ABE ◽  
Hiroko TAKANO
Keyword(s):  
Epithelial Cells ◽  
Initial Segment ◽  
Efferent Duct ◽  
Epididymal Duct

scholarly journals Castration causes an increase in lysosomal size and upregulation of cathepsin D expression in principal cells along with increased secretion of procathepsin D and prosaposin oligomers in adult rat epididymis

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250454
Author(s):  
Lorena Carvelli ◽  
Andrea Carolina Aguilera ◽  
Leila Zyla ◽  
Laura Lucía Pereyra ◽  
Carlos R. Morales ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Complex Formation ◽  
Cathepsin D ◽  
Initial Segment ◽  
Lysosomal Enzymes ◽  
Sperm Maturation ◽  
Principal Cells ◽  
Adult Rat ◽  
Rat Epididymis ◽  
Lysosomal Proteins

In the epididymis, lysosomal proteins of the epithelial cells are normally targeted from the Golgi apparatus to lysosomes for degradation, although their secretion into the epididymal lumen has been documented and associated with sperm maturation. In this study, cathepsin D (CatD) and prosaposin (PSAP) were examined in adult epididymis of control, and 2-day castrated rats without (Ct) and with testosterone replacement (Ct+T) to evaluate their expression and regulation within epididymal epithelial cells. By light microscope-immunocytochemistry, a quantitative increase in size of lysosomes in principal cells of Ct animals was noted from the distal initial segment to the proximal cauda. Androgen replacement did not restore the size of lysosomes to control levels. Western blot analysis revealed a significant increase in CatD expression in the epididymis of Ct animals, which suggested an upregulation of its expression in principal cells; androgens restored levels of CatD to that of controls. In contrast, PSAP expression in Ct animals was not altered from controls. Additionally, an increase in procathepsin D levels was noted from samples of the epididymal fluid of Ct compared to control animals, accompanied by an increased complex formation with PSAP. Moreover, an increased oligomerization of prosaposin was observed in the epididymal lumen of Ct rats, with changes reverted to controls in Ct+T animals. Taken together these data suggest castration causes an increased uptake of substrates that are acted upon by CatD in lysosomes of principal cells and in the lumen by procathepsin D. These substrates may be derived from apoptotic cells noted in the lumen of proximal regions and possibly by degenerating sperm in distal regions of the epididymis of Ct animals. Exploring the mechanisms by which lysosomal enzymes are synthesized and secreted by the epididymis may help resolve some of the issues originating from epididymal dysfunctions with relevance to sperm maturation.

scholarly journals AN ULTRACYTOCHEMICAL AND ULTRASTRUCTURAL STUDY OF EPITHELIAL CELLS IN DUCTULI EFFERENTES OF CHINESE HAMSTER

1971 ◽  
Vol 19 (12) ◽  
pp. 766-774 ◽  
Author(s):  
MASAO YOKOYAMA ◽  
JEFFREY P. CHANG
Keyword(s):  
Epithelial Cells ◽  
Golgi Apparatus ◽  
Phosphatase Activity ◽  
Plasma Membranes ◽  
Chinese Hamster ◽  
Fluid Composition ◽  
Ciliated Cells ◽  
Efferent Duct ◽  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity

Ultrastructural and ultracytochemical features of the efferent duct of Chinese hamster have been studied. The ducts are composed of two main types of epithelial cells, ciliated and nonciliated. Distinct structural and cytochemical characteristics of these cells are apparent. Presence of fibrogranular complex which is supposedly related to basal body replication was demonstrated in ciliated cells for the first time in this tissue. Thiamine pyrophosphatase activity in Golgi apparatus, acid phosphatase activity in Golgi apparatus and lysosomes and alkaline phosphatase activity on basal plasma membranes of both ciliated and nonciliated cells have been localized. However, thiamine pyrophosphatase activity was seen only on the luminal surface, apical vacuole and apical tubular structure of nonciliated cells but not on the surface of ciliated cells. Similarly, horseradish peroxidase was absorbed only by nonciliated cells. The cytochemical and ultrastructural differences between the two types of cells indicate a functional specialization. The results indicate that the ciliated cells are concerned with the transportation of the sperms and that the nonciliated cells are concerned with the regulation of fluid composition in the duct since the latter are capable of both secretion and absorption.

The structure and function of the epididymis. 2. The histogenesis of the rat epididymis.

1959 ◽  
Vol 7 (1) ◽  
pp. 22 ◽  
Author(s):  
BL Reid
Keyword(s):  
Connective Tissue ◽  
Ciliated Cells ◽  
Efferent Duct ◽  
White Rats ◽  
Efferent Ducts ◽  
Caudal Portion ◽  
Rat Epididymis ◽  
Nuclear Differentiation ◽  
And Function ◽  
Epididymal Duct

Observations were made on the epididymides of young white rats of the following ages: 3, 21, 28, 32, 37, 39, 56, 72, 96, 110 days. Both efferent ducts and epididymal duct are undifferentiated at 21 days with a similar cuboidal epithelium. The connective tissue coat of the efferent ducts is one cell thick whereas that of the epididymal duct is two or three cells thick. The possible involvement of the connective tissue in the process of histogenesis is discussed. Differentiation within the epididymal duct commences at 28 days when the epithelium in the cephalic portion is tall and that in the caudal portion of the head and remainder of the tail is tall with isolated segments of low columnar epithelium. The latter epithelium is associated with a wider lumen which evidently becomes continuous down the duct. In the efferent ducts at this stage ciliated cells have appeared. Differentiation of the cephalic portion of the head is completed rapidly by the 37th day but that of the caudal portion of the head and tail of the organ is completed only at the 96th day. In certain zones, histodifferentiation is accompanied by obvious nuclear differentiation. Spermatozoa first appear in the testis at 56 days but do not enter and fill the epididymal ducts until 72 days. There is evidence of an outflow of fluid from the testis which carries spermatocytes and spermatids into the duct at 32 days. The changes in the epithelium of the efferent duct, the epididymis, and the deferent duct from the 3rd to the 110th day are tabulated.

Isolation and culture of epithelial cells from rat ductuli efferentes and initial segment epididymidis

1998 ◽  
Vol 30 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Y.C. Chen ◽  
D. Bunick ◽  
J.M. Bahr ◽  
G.R. Klinefelter ◽  
R.A. Hess
Keyword(s):  
Epithelial Cells ◽  
Initial Segment ◽  
Ductuli Efferentes

scholarly journals Tubule-Containing Inclusions in the Epithelial Cells of the Mouse Epididymal Duct

1983 ◽  
Vol 46 (1) ◽  
pp. 69-77 ◽  
Author(s):  
Kazuhiro ABE ◽  
Hiroko TAKANO ◽  
Takashi ITO
Keyword(s):  
Epithelial Cells ◽  
Epididymal Duct

scholarly journals Androgens are essential for epithelial cell recovery after efferent duct ligation in the initial segment of the mouse epididymis†

2019 ◽  
Vol 102 (1) ◽  
pp. 76-83
Author(s):  
Bongki Kim ◽  
Sylvie Breton
Keyword(s):  
Epithelial Cell ◽  
Initial Segment ◽  
Recovery Phase ◽  
Progressive Increase ◽  
Basal Cells ◽  
Cell Recovery ◽  
Cell Degeneration ◽  
Efferent Duct ◽  
Duct Ligation ◽  
Repair Ability

Abstract Efferent duct ligation (EDL) induces epithelial cell degeneration followed by regeneration in the epididymal initial segment. We tested here the role of androgens in the recovery phase. EDL was performed at post-natal weeks (PNW) 3, 4, 5, 6, and 7, and apoptotic and proliferating epithelial cells were quantified 24 h, and at days 2 and 2.5 post-EDL, respectively. A progressive increase in the number of apoptotic basal cells (BCs) and principal cells (PCs) was detected from PNW3 to 6, 24 h after EDL. Two days after EDL, no increase in proliferating BCs and PCs was observed at PNW3 and 4, despite the induction of apoptosis by EDL. A progressive increase in the number of proliferating BCs was then observed from PNW5 to 6, while the number of proliferating PCs remained low. 2.5 days after EDL, the number of proliferating BCs and PCs remained low at PNW3, 4, and 5, but a marked increase in the number of proliferating PCs was observed at PNW6. Flutamide pretreatment for 3 weeks followed by EDL at PNW7 dramatically decreased the number of proliferating BCs on EDL day 2, and the number of proliferating PCs on EDL day 2.5, compared to controls. We conclude that (1) BCs are the first to show recovery after EDL, followed by PCs; (2) androgens are essential for BC and PC repair after injury in the postpubertal epididymis; and (3) the prepubertal epididymis lacks repair ability following injury.

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