scholarly journals Differential expression and regulation of prostaglandin E synthases in the mouse ovary during sexual maturation and luteal development

2006 ◽  
Vol 189 (1) ◽  
pp. 89-101 ◽  
Author(s):  
Tong Sun ◽  
Wen-Bo Deng ◽  
Hong-Lu Diao ◽  
Hua Ni ◽  
Yu-Yan Bai ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Chorionic Gonadotropin ◽  
Granulosa Cells ◽  
Sexual Maturation ◽  
Mrna Level ◽  
Follicular Development ◽  
Prostaglandin E ◽  
Female Reproduction ◽  
Mouse Ovary ◽  
Equine Chorionic Gonadotropin

Prostaglandin (PGE) 2 is the most common prostanoid and plays an important role in female reproduction. The aim of this study was to examine the expression and regulation of microsomal (m) PGE synthase (PGES)-1 and cytosolic (c) PGES in the mouse ovary during sexual maturation, gonadotropin treatment and luteal development by in situ hybridization and immunohistochemistry. Both mPGES-1 mRNA signals and immunostaining were localized in the granulosa cells, but not in the thecal cells and oocytes. cPGES mRNA signals were localized in both granulosa cells and oocytes, whereas cPGES immunostaining was exclusively localized in the oocytes. In our superovulated model of immature mice, there was a basal level of mPGES-1 mRNA signals in the granulosa cells at 48 h after equine chorionic gonadotropin (eCG) treatment. mPGES-1 mRNA level was induced by human chorionic gonadotropin (hCG) treatment for 0.5 h, whereas mPGES-1 immunostaining was slightly induced at 0.5 h after hCG treatment and reached a maximal level at 3 h after hCG treatment. eCG treatment had no obvious effects on either cPGES mRNA signals or immunostaining. A strong level of cPGES immunostaining was present in both unstimulated and eCG-treated groups. Both mPGES-1 mRNA signals and immunostaining were highly detected in the corpus luteum 2 days post-hCG injection and declined from days 3 to 7 post-hCG injection. cPGES immunostaining was at a basal level or not detectable from days 1 to 7 after hCG injection and was highly expressed in the corpus luteum from days 9 to 15 post-hCG injection. PGE2 biosynthesized through the mPGES-1 pathway may be important for follicular development, ovulation and luteal formation.

187 EXPRESSION AND REGULATION OF THE FIBROBLAST GROWTH FACTOR GENE FAMILY DURING MOUSE FOLLICULAR DEVELOPMENT

2013 ◽  
Vol 25 (1) ◽  
pp. 243
Author(s):  
S. Furukawa ◽  
K. Naito ◽  
K. Sugiura
Keyword(s):  
Gene Family ◽  
Granulosa Cells ◽  
Follicular Development ◽  
Cumulus Cells ◽  
Mrna Levels ◽  
Fibroblast Growth ◽  
Mouse Ovary ◽  
Expression Levels ◽  
Before And After ◽  
Equine Chorionic Gonadotropin

Recent studies have shown the critical roles of fibroblast growth factors (FGFs), including FGF8 produced by oocytes, in regulating follicular development. However, the expression and regulation of the FGF gene family, which consists of 22 ligands and 4 receptors, in the mouse ovary have not been well understood. The aim of the present study was to assess the expression and regulation of FGF ligands and receptors in the mouse ovary. Transcript levels of FGF ligands and receptors in immature (3-week-old) and adult (7- to 8-week-old) ovaries as well as other tissues of B6/DBA2F1 mice were analysed with RT-PCR. Furthermore, expression levels of FGF receptors in cumulus cells (CC) and mural granulosa cells (MG) before and after equine chorionic gonadotropin (eCG) treatment were determined with RT-quantitative PCR. Among 21 FGF ligands examined, 12 and 9 transcripts were detectable in immature and adult ovaries, respectively. More FGF ligands were detected in ovary, testis, heart, and brain compared to other tissues, including liver and spleen. Transcripts of all 4 FGF receptors (Fgfr1–4) were detectable in both immature and adult ovaries. Expression levels of Fgfr1 and Fgfr2 were significantly higher in MG compared with CC before and after the eCG treatment. Levels of Fgfr4 were comparable between MG and CC before the eCG treatment, but became significantly different with higher expression levels in MG after the eCG treatment. Fgfr3 transcripts were barely detectable in CC and MG. Overall levels of Fgfr1 in granulosa cells (CC and MG) were downregulated by eCG treatment, whereas those of Fgfr2 and Fgfr4 were upregulated. In summary, many FGF ligands are expressed, at least in mRNA levels, in mouse ovaries. Moreover, the expression levels of Fgfr transcripts in granulosa cells are dynamically regulated during follicular development.

Hormonal regulation of prostaglandin F2 alpha receptor gene expression in mouse ovary

1996 ◽  
Vol 271 (4) ◽  
pp. E686-E693
Author(s):  
J. Sugatani ◽  
Y. Masu ◽  
M. Nishizawa ◽  
K. Sakamoto ◽  
T. Houtani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Corpus Luteum ◽  
Hormonal Regulation ◽  
Receptor Gene ◽  
Mrna Level ◽  
Dependent Manner ◽  
Mouse Ovary ◽  
Receptor Mrna ◽  
Prostaglandin F2 Alpha ◽  
Receptor Gene Expression

In this study we examined regulation by pituitary gonadotropins of the prostaglandin F2 alpha (PGF2 alpha) receptor gene expression in the mouse ovary. Administration of pregnant mare serum gonadotropin (PMSG) to 35-day-old mice in the diestrus phase stimulated the ovary and enhanced the production of progesterone at 1 h PMSG also increased the ovarian PGF2 alpha receptor mRNA level in a time-dependent manner, reaching a sixfold maximum at 1 h. These actions of PMSG were mimicked by human chorionic gonadotropin (hCG), follicle-stimulating hormone (FSH), and cholera toxin, all of which elevate intracellular adenosine 3',5'-cyclic monophosphate (cAMP). In situ hybridization revealed that PGF2 alpha receptor mRNA was localized to the corpus luteum, but the intensity of staining varied among corpora lutea in the same ovary. Exogenous PGF2 alpha inhibited the PMSG-stimulated progesterone production. These results demonstrate that gonadotropins may induce the expression of the PGF2 alpha receptor gene in luteal cells of the corpus luteum, probably by acting through a cAMP-mediated pathway, and that expression of the PGF2 alpha receptor may be functionally associated with the decrease in serum progesterone level.

scholarly journals Female Fertility Is Reduced in Mice Lacking Ca2+/Calmodulin-Dependent Protein Kinase IV**This work was supported by an NIH Medical Scientist Training Program award (to J.Y.W.) and NIH Grants HD-07503 (to A.R.M.) and HD-1272 (to J.S.R.)

Endocrinology ◽  
2000 ◽  
Vol 141 (12) ◽  
pp. 4777-4783 ◽  
Author(s):  
Joy Y. Wu ◽  
Ignacio J. Gonzalez-Robayna ◽  
JoAnne S. Richards ◽  
Anthony R. Means
Keyword(s):  
Protein Kinase ◽  
Granulosa Cells ◽  
Critical Role ◽  
Follicular Development ◽  
Female Fertility ◽  
Female Reproduction ◽  
Dependent Protein Kinase ◽  
Medical Scientist ◽  
Dependent Protein ◽  
Medical Scientist Training Program

Abstract Ca2+/calmodulin-dependent protein kinase IV (CaMKIV) is a serine/threonine protein kinase with limited tissue distribution. CaMKIV is highly expressed in the testis, where it is found in transcriptionally inactive elongating spermatids. We have recently generated mice deficient in CaMKIV. In the absence of CaMKIV, the exchange of sperm nuclear basic proteins in male spermatids is impaired, resulting in male infertility secondary to defective spermiogenesis. The involvement of CaMKIV in female fertility has not been addressed. Here we report that female fertility is markedly reduced in CaMKIV-deficient mice due to impaired follicular development and ovulation. CaMKIV is expressed in the ovary, where it is localized in granulosa cells. We further find that in cultured granulosa cells, CaMKIV expression and subcellular localization are hormonally regulated. As granulosa cells differentiate, CaMKIV levels decrease and the kinase translocates from the nucleus into the cytoplasm. Our results demonstrate a critical role for CaMKIV in female reproduction and point to a potential function in granulosa cell differentiation.

Microvascularization of corpus luteum of bovine treated with equine chorionic gonadotropin

2015 ◽  
Vol 78 (9) ◽  
pp. 747-753 ◽  
Author(s):  
Carlos Eduardo Bezerra Moura ◽  
Nathia Nathaly Rigoglio ◽  
Janine Karla França S. Braz ◽  
Marcello Machado ◽  
Pietro S. Baruselli ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Chorionic Gonadotropin ◽  
Equine Chorionic Gonadotropin

Follicular development in pregnant cows after the administration of equine chorionic gonadotropin (eCG): A new insight

2009 ◽  
Vol 31 (4) ◽  
pp. 536-542 ◽  
Author(s):  
Annalisa Rizzo ◽  
Massimo Spedicato ◽  
Giuseppe Minoia ◽  
Maddalena Mutinati ◽  
Mario Cinone ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Follicular Development ◽  
Equine Chorionic Gonadotropin

Effects of presynchronization and eCG on pregnancy rates to GnRH-based, fixed-time artificial insemination in beef heifers

2010 ◽  
Vol 90 (1) ◽  
pp. 23-34 ◽  
Author(s):  
J A Small ◽  
M G Colazo ◽  
J P Kastelic ◽  
N E Erickson ◽  
R J Mapletoft
Keyword(s):  
Chorionic Gonadotropin ◽  
Artificial Insemination ◽  
Follicular Development ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Preovulatory Follicle ◽  
Beef Heifers ◽  
Plasma Progesterone ◽  
Follicle Diameter ◽  
Equine Chorionic Gonadotropin

Three experiments were conducted to determine the effects of presynchronization and treatment with equine chorionic gonadotropin (eCG) on corpus luteum (CL) and ovarian follicular development, plasma progesterone concentrations, and pregnancy rates in beef heifers subjected to a gonadotropin releasing hormone (GnRH)-based, fixed-timed AI (TAI) protocol. All heifers were given GnRH on day 0, prostaglandin F2α (PGF) on day 7, and a second GnRH on day 9 concurrent with TAI (54 h after PGF). In exp. 1 (N = 148), presynchronization with PGF (days -22 and -11) decreased the percentage of heifers with non-luteal plasma progesterone concentrations on day 0 (5.4 vs 29.7%) and day 7 (0 vs 11.6%; P < 0.05), but not on day 9 (74.3 vs. 66.2%; P > 0.20), and reduced the number of heifers in estrus and bred before TAI (P < 0.05). Although presynchronization reduced preovulatory follicle diameter (12.9 ± 0.3 vs. 14.9 ± 0.3 mm; mean ± SEM; P < 0.01), it did not affect TAI pregnancy rates (36.5 vs. 29.7%; P > 0.20). In exp. 2, heifers (N = 128) were presynchronized with melengestrol acetate (MGA) (days -27 to -12), and received a controlled internal drug release (CIDR) on day 0; on day 7, half were given 300 IU of eCG at CIDR removal. Treatment with eCG tended to increase preovulatory follicle diameter in heifers that did not ovulate to GnRH on day 0 (P = 0.06), but did not affect the percentage of heifers with non-luteal plasma progesterone concentrations on day 9 (57.8 vs. 57.8%) or TAI pregnancy rates (48.4 vs. 53.1%; P > 0.20). Experiment 3 was a 2 × 2 factorial arrangement of presynchronization (PGF concurrent with a CIDR on day -7) and eCG treatments (on day 7) applied to heifers in three herds (A, N = 150, B, N = 260 and C, N = 40). All heifers had a once-used CIDR from days 0 to 7. Presynchronization increased the percentage of heifers (Herd A) with low-luteal plasma progesterone concentrations on day 0 (70.7 vs. 22.7%) and day 7 (90.7 vs. 53.3%; P < 0.01), but did not affect the percentage of heifers with non-luteal concentrations of progesterone on day 9 (97.3 vs. 93.3%; P > 0.20). Combined for all herds, presynchronization reduced the prevalence of a CL on day 0 (23.5 vs. 73.7%; P < 0.01), and increased the prevalence of follicles ≥ 10 mm on day 7 (96.8 vs. 86.7%; P < 0.01); however, TAI pregnancy rates (195/439 = 44.4%) were not improved by either presynchronization or eCG treatment (P > 0.20).Key words: Presynchronization, equine chorionic gonadotropin, GnRH, fixed-time artificial insemination, progesterone

Basigin expression and regulation in mouse ovary during the sexual maturation and development of corpus luteum

2004 ◽  
Vol 68 (2) ◽  
pp. 135-141 ◽  
Author(s):  
Hong Chang ◽  
Hua Ni ◽  
Xing-Hong Ma ◽  
Li-Bin Xu ◽  
Kenji Kadomatsu ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Sexual Maturation ◽  
Mouse Ovary

scholarly journals Expression and Localization of Secreted Frizzled-Related Protein-4 in the Rodent Ovary: Evidence for Selective Up-Regulation in Luteinized Granulosa Cells

Endocrinology ◽  
2003 ◽  
Vol 144 (10) ◽  
pp. 4597-4606 ◽  
Author(s):  
Minnie Hsieh ◽  
Sabine M. Mulders ◽  
Robert R. Friis ◽  
Arun Dharmarajan ◽  
JoAnne S. Richards
Keyword(s):  
Chorionic Gonadotropin ◽  
Granulosa Cells ◽  
Human Chorionic Gonadotropin ◽  
Follicular Development ◽  
Corpora Lutea ◽  
Related Protein ◽  
Rich Domain ◽  
And Function ◽  
Periovulatory Follicles

Secreted frizzled-related protein-4 (sFRP-4) belongs to a family of soluble proteins that have a Frizzled-like cysteine-rich domain and function as modulators of Wnt-Frizzled (Fz) signals. As several Wnts and Fz are expressed at defined stages of follicular development in rodent ovaries, these studies were undertaken to evaluate the hormone-regulated expression and localization of sFRP-4. In the mouse ovary, the expression of sFRP-4 mRNA was up-regulated in granulosa cells of large antral follicles after human chorionic gonadotropin administration and was also elevated in corpora lutea, as determined by RT-PCR and in situ hybridization analyses. In hypophysectomized rat ovaries, sFRP-4 expression was similarly induced by human chorionic gonadotropin and further up-regulated by PRL. PRL also stimulated the secretion of sFRP-4 protein from luteinized rat granulosa cells in culture. Therefore, regulation of sFRP-4 by LH and PRL may be important for modulating Fz-1, which is known to be expressed in periovulatory follicles, and Wnt-4/Fz-4, which are expressed in corpora lutea.

Ultrastructural localisation of calcium deposits in the mouse ovary

2003 ◽  
Vol 15 (8) ◽  
pp. 415 ◽  
Author(s):  
M. Sedmíková ◽  
R. Rajmon ◽  
J. Petr ◽  
M. Vaňková ◽  
J. Rozinek ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Follicular Development ◽  
Mouse Ovary ◽  
Antral Follicles ◽  
Mouse Oocytes ◽  
Adult Mice ◽  
Calcium Deposits ◽  
Cellular Compartments ◽  
Meiotic Competence

Follicle-enclosed mouse oocytes contain numerous calcium deposits. The ultrastructural distribution of calcium deposits in the nuclei, mitochondria and cytoplasm of mouse oocytes and granulosa cells of primary, secondary and antral follicles was examined using the combined oxalate–pyroantimonate method. The mitochondria of oocytes from all types of follicles had the highest levels of calcium deposits of all oocyte compartments, with the exception of primary follicles, in which oocyte nuclei contained the same level of calcium deposits as the mitochondria. Calcium deposits in the cytoplasm of oocytes from primary follicles were significantly lower than those in the cytoplasm of oocytes from secondary and antral follicles. Calcium deposits in the cytoplasm of granulosa cells were significantly lower than calcium deposits in the mitochondria of granulosa cells and this difference persisted throughout all categories of follicles. Calcium deposits in the nuclei of granulosa cells did not differ from levels in the mitochondria in primary and secondary follicles. In contrast, the nuclei of granulosa cells from antral follicles had lower levels of calcium deposits than the mitochondria. The differences observed in calcium deposits in various cellular compartments in oocytes and granulosa cells in the follicles of ovaries of adult mice can be attributed to their acquisition of meiotic competence and follicular development.

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