Molecular Detection ofHemolivia(Apicomplexa: Haemogregarinidae) from Ticks of North AfricanTestudo graeca(Testudines: Testudinidae) and an Estimation of Their Phylogenetic Relationships Using 18S rRNA Sequences

2013 ◽  
Vol 80 (2) ◽  
pp. 292-296 ◽  
Author(s):  
D. J. Harris ◽  
Eva Graciá ◽  
Fátima Jorge ◽  
Joao P. M. C. Maia ◽  
Ana Perera ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
18S Rrna ◽  
Molecular Detection ◽  
Rrna Sequences

The phylogenetic relationships of the anaerobic chytridiomycetous gut fungi (Neocallimasticaceae) and the Chytridiomycota. I. Cladistic analysis of rRNA sequences

1992 ◽  
Vol 70 (9) ◽  
pp. 1738-1746 ◽  
Author(s):  
Jinliang Li ◽  
I. Brent Heath
Keyword(s):  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Trees ◽  
18S Rrna ◽  
Cladistic Analysis ◽  
Anaerobic Fungi ◽  
Confidence Limits ◽  
Maximum Likelihood Algorithm ◽  
Rrna Sequences ◽  
Partial 18S

To clarify the phylogenetic relationships of the Chytridiomycota and the anaerobic fungi from the rumen and caecum of herbivorous animals, we analyzed the partial 18S rRNA sequences from 28 species ranging from protists to mammals and internal transcribed spacer 1 (ITS1) and its adjacent sequences from four gut fungi and one chytrid by using three algorithms from the Phylogeny Inference Package (PHYLIP). To get the confidence limits for each branch, we applied bootstrapping for each algorithm. Our analysis on partial 18S rRNA sequences shows that the Chytridiomycota are clustered with other fungi with 98, 76, and 30% confidences in the Fitch–Margoliash, neighbour-joining, and maximum parsimony algorithms. None of these three algorithms place any of 17 protists from 12 phyla with the fungi, including the chytrids. The same analysis also shows that the Spizellomycetales and Chytridiales cluster with the gut fungi but does not identify which order is closest to them. These results suggest that the Chytridiomycota, including the gut fungi, are indeed fungi but the gut fungi might not belong to the Spizellomycetales. The phylogenetic trees generated by the above three algorithms, plus the maximum likelihood algorithm, based on ITS1 and its adjacent regions show that Anaeromyces is more distant from Orpinomyces, Neocallimastix, and Piromyces. However, they failed to determine the relationships among the last three genera. Key words: Chytridiomycota, gut fungi, rumen fungi, phylogeny, rRNA sequences.

Phylogenetic relationships within the Octocorallia (Cnidaria: Anthozoa) based on nuclear 18S rRNA sequences

2001 ◽  
Vol 138 (2) ◽  
pp. 235-246 ◽  
Author(s):  
E. A. Berntson ◽  
F. M. Bayer ◽  
A. G. McArthur ◽  
S. C. France
Keyword(s):  
Phylogenetic Relationships ◽  
18S Rrna ◽  
Rrna Sequences

Whale falls, multiple colonisations of the deep, and the phylogeny of Hesionidae (Annelida)

2015 ◽  
Vol 29 (2) ◽  
pp. 105 ◽  
Author(s):  
Mindi Summers ◽  
Fredrik Pleijel ◽  
Greg W. Rouse
Keyword(s):  
New Species ◽  
Maximum Likelihood ◽  
16S Rrna ◽  
Deep Sea ◽  
Phylogenetic Relationships ◽  
18S Rrna ◽  
Shallow Waters ◽  
28S Rrna ◽  
Mitochondrial Cytochrome ◽  
New Members

Phylogenetic relationships within Hesionidae Grube, 1850 are assessed via maximum parsimony and maximum likelihood analyses of mitochondrial (cytochrome c oxidase subunit I and 16S rRNA) and nuclear (18S rRNA, and 28S rRNA) data. The analyses are based on 42 hesionid species; six of these being new species that are described here. The new species, all from deep (>200 m depth) benthic environments (including whale falls) in the eastern Pacific, are Gyptis shannonae, sp. nov., Neogyptis julii, sp. nov., Sirsoe sirikos, sp. nov., Vrijenhoekia ketea, sp. nov., Vrijenhoekia falenothiras, sp. nov., and Vrijenhoekia ahabi, sp. nov. The molecular divergence among the new members of Vrijenhoekia is pronounced enough to consider them cryptic species, even though we cannot distinguish among them morphologically. Our results also showed that the subfamily Hesioninae Grube, 1850, as traditionally delineated, was paraphyletic. We thus restrict Hesioninae to include only Hesionini Grube, 1850 and refer the remaining members to Psamathinae Pleijel, 1998. The present study increases the number of hesionid species associated with whale falls from one to six and markedly increases the number of described deep-sea hesionid taxa. There appear to have been multiple colonisations of the deep sea from shallow waters by hesionids, though further sampling is warranted.

scholarly journals Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil

2018 ◽  
Vol 27 (4) ◽  
pp. 505-513 ◽  
Author(s):  
Anna Cláudia Baumel Mongruel ◽  
Priscila Ikeda ◽  
Keyla Carstens Marques de Sousa ◽  
Jyan Lucas Benevenute ◽  
Margarete Kimie Falbo ◽  
...  
Keyword(s):  
18S Rrna ◽  
Molecular Detection ◽  
Phylogenetic Analyses ◽  
Southern Brazil ◽  
Conventional Pcr ◽  
Pathogenic Potential ◽  
Vector Borne ◽  
Pcr Assays ◽  
Etiological Agents ◽  
Positive Results

Abstract Arthropod-borne pathogens are medically important because of their ability to cause diseases in their hosts. The purpose of this study was to detect the occurrence of Ehrlichia spp., piroplasmids and Hepatozoon spp. in dogs with anemia and thrombocytopenia in southern Brazil. EDTA-whole blood was collected from 75 domestic dogs presenting anemia or/and thrombocytopenia from Guarapuava, state of Paraná, Brazil. DNA samples were subjected to conventional PCR assays for Ehrlichia spp. (dsb), piroplasmids (18S rRNA) and Hepatozoon spp. (18S rRNA), followed by sequencing and phylogenetic analyses. Among the 75 dogs, one (1.33%) was positive for Hepatozoon sp. and six (8%) were positive for piroplasmids in 18S rRNA cPCR assays. None of the dogs showed positive results in Ehrlichia spp.-cPCR targeting dsb gene. The phylogenetic analyses revealed that three piroplasm sequences were clustered with Rangellia vitalii, while one sequence was grouped with B. vogeli. The only sequence obtained from Hepatozoon spp.-PCR protocol was pooled with H. canis. Therefore, there is urgent need for differential molecular diagnosis of the two piroplasm species cited as etiological agents in clinical cases of canine hemoparasitic diseases, given the higher pathogenic potential of R. vitalii than of B. vogeli.

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