Genetic Analysis of Small-Subunit Ribosomal Rna, Internal Transcribed Spacer 2, and ATP Synthase Subunit 8 of Trichuris trichiura Ancient DNA Retrieved from the 15th to 18th Century Joseon Dynasty Mummies' Coprolites from Korea

10.1645/19-31 ◽  
2019 ◽  
Vol 105 (4) ◽  
pp. 539 ◽  
Author(s):  
Jong Ha Hong ◽  
Min Seo ◽  
Chang Seok Oh ◽  
Dong Hoon Shin
Keyword(s):  
Genetic Analysis ◽  
Atp Synthase ◽  
Ribosomal Rna ◽  
Ancient Dna ◽  
Internal Transcribed Spacer ◽  
18Th Century ◽  
Small Subunit ◽  
Trichuris Trichiura ◽  
Internal Transcribed Spacer 2 ◽  
Joseon Dynasty

scholarly journals An ITS-based phylogenetic framework for the genus Vorticella : finding the molecular and morphological gaps in a taxonomically difficult group

2013 ◽  
Vol 280 (1771) ◽  
pp. 20131177 ◽  
Author(s):  
Ping Sun ◽  
John C. Clamp ◽  
Dapeng Xu ◽  
Bangqin Huang ◽  
Mann Kyoon Shin ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
Small Subunit ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
Branching Order ◽  
Close Relationship ◽  
Molecular Phylogenetic ◽  
Phylogenetic Framework

Vorticella includes more than 100 currently recognized species and represents one of the most taxonomically challenging genera of ciliates. Molecular phylogenetic analysis of Vorticella has been performed so far with only sequences coding for small subunit ribosomal RNA (SSU rRNA); only a few of its species have been investigated using other genetic markers owing to a lack of similar sequences for comparison. Consequently, phylogenetic relationships within the genus remain unclear, and molecular discrimination between morphospecies is often difficult because most regions of the SSU rRNA gene are too highly conserved to be helpful. In this paper, we move molecular systematics for this group of ciliates to the infrageneric level by sequencing additional molecular markers—fast-evolving internal transcribed spacer (ITS) regions—in a broad sample of 66 individual samples of 28 morphospecies of Vorticella collected from Asia, North America and Europe. Our phylogenies all featured two strongly supported, highly divergent, paraphyletic clades (I, II) comprising the morphologically defined genus Vorticella . Three major lineages made up clade I, with a relatively well-resolved branching order in each one. The marked divergence of clade II from clade I confirms that the former should be recognized as a separate taxonomic unit as indicated by SSU rRNA phylogenies. We made the first attempt to elucidate relationships between species in clade II using both morphological and multi-gene approaches, and our data supported a close relationship between some morphospecies of Vorticella and Opisthonecta , indicating that relationships between species in the clade are far more complex than would be expected from their morphology. Different patterns of helix III of ITS2 secondary structure were clearly specific to clades and subclades of Vorticella and, therefore, may prove useful for resolving phylogenetic relationships in other groups of ciliates.

scholarly journals Morphological and molecular studies on Resinicium s. str.

2007 ◽  
Vol 85 (4) ◽  
pp. 420-436 ◽  
Author(s):  
Karen K. Nakasone
Keyword(s):  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Small Subunit ◽  
Caribbean Region ◽  
Sequence Analyses ◽  
Internal Transcribed Spacer Region ◽  
Resinicium Bicolor ◽  
Molecular Studies ◽  
The Caribbean

Resinicium Parmasto is typified by Resinicium bicolor (Alb. & Schwein.: Fr.) Parm., (Hymenochaetales, Basidiomycota), a readily recognized and widely distributed corticioid, lignicolous species in the northern hemisphere. Five new species of Resinicium closely allied to R. bicolor from the Caribbean region are described and illustrated: Resinicium confertum Nakasone, sp. nov., Resinicium monticola Nakasone, sp. nov., Resinicium mutabile Nakasone, sp. nov., Resinicium rimulosum Nakasone, sp. nov., and Resinicium tenue Nakasone, sp. nov. In addition, R. bicolor and Resinicium friabile Hjortstam & Melo are redescribed and illustrated. Sequence analyses of the internal transcribed spacer region support the recognition of the new taxa. Further, nuclear small subunit ribosomal RNA sequence data support the recognition of two groups of Resinicium species: the Resinicium s. str. group, including R. bicolor, and the Resinicium s.l. group that includes Resinicium furfuraceum (Bres.) Parm. The genus Resinicium is redefined and a key to the Resinicium s. str. species is provided.

Morphological and molecular characterisation of three species of Paratylenchus Micoletzky, 1922 (Tylenchida: Paratylenchidae) from China, with a first description of the male P. rostrocaudatus

Nematology ◽  
2018 ◽  
Vol 20 (9) ◽  
pp. 837-850 ◽  
Author(s):  
Kan Zhuo ◽  
Xingtong Liu ◽  
Ye Tao ◽  
Honghong Wang ◽  
Borong Lin ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
New Records ◽  
Large Subunit ◽  
Small Subunit ◽  
Parasitic Nematodes ◽  
Molecular Characterisation ◽  
Plant Parasitic Nematodes ◽  
First Time ◽  
Plant Parasitic

Summary In a recent survey of plant-parasitic nematodes associated with forest in China, three species of Paratylenchus, including P. aculentus, P. leptos and P. rostrocaudatus, were found. Paratylenchus leptos and P. rostrocaudatus are new records for China and the male of P. rostrocaudatus is described for the first time. Morphological descriptions, morphometrics and light microscopic photos are provided for these three species. Molecular characterisation of these species using ribosomal RNA (rRNA) D2-D3 expansion domains of large subunit (LSU D2-D3), internal transcribed spacer (ITS) and small subunit (SSU) sequences are also given. Except for the ITS sequence of P. aculentus, other sequences were amplified for the first time. Phylogenetic relationships within the genus Paratylenchus are discussed as inferred from the analyses of LSU D2-D3, ITS and SSU.

Polymorphism analysis of the internal transcribed spacer and small subunit of ribosomal RNA genes of Leishmania mexicana

2002 ◽  
Vol 88 (10) ◽  
pp. 918-925 ◽  
Author(s):  
Miriam Berzunza-Cruz ◽  
Nallely Cabrera ◽  
Marco Crippa-Rossi ◽  
Tayde Sosa Cabrera ◽  
Ruy Pérez-Montfort ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Small Subunit ◽  
Ribosomal Rna Genes ◽  
Polymorphism Analysis ◽  
Leishmania Mexicana ◽  
Rna Genes

scholarly journals Two new calcicolous caloplacoid lichens from South Korea, with a taxonomic key to the species of Huriella and Squamulea

MycoKeys ◽  
2021 ◽  
Vol 84 ◽  
pp. 35-55
Author(s):  
Beeyoung Gun Lee ◽  
Jae-Seoun Hur
Keyword(s):  
New Species ◽  
South Korea ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Small Subunit ◽  
Similar Species ◽  
Molecular Analyses ◽  
Tip Cells ◽  
Large Subunit Ribosomal Rna

Pyrenodesmia rugosa Lee & Hur and Huriella aeruginosa Lee & Hur are described as new lichen-forming fungi from a calcareous mountain of South Korea. Pyrenodesmia rugosa is distinguishable from Pyrenodesmia micromontana (Frolov, Wilk & Vondrák) Hafellner & Türk, the most similar species, by thicker thallus, rugose areoles, larger apothecia, shorter hymenium, shorter hypothecium and narrower tip cells of paraphyses. Huriella aeruginosa, the second new species, differs from ‘Squamulea’ chelonia Bungartz & Søchting by dark greenish-grey to grey thallus without pruina, gold to yellow-brown epihymenium, larger ascospores and thallus K– and KC– reaction. Molecular analyses employing internal transcribed spacer (ITS), mitochondrial small subunit (mtSSU) and nuclear large subunit ribosomal RNA (LSU) sequences strongly support the two caloplacoid species to be distinct in their genera. A surrogate key is provided to assist in the identification of all 20 taxa in Huriella and Squamulea.

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