scholarly journals Identification and Typing of Miso and Soy Sauce Fermentation Yeasts,Candida etchellsiiandC. versatilis, Based on Sequence Analyses of the D1D2 Domain of the 26S Ribosomal RNA Gene, and the Region of Internal Transcribed Spacer 1, 5.8S Ribosomal RNA Gene and Internal Transcribed Spacer 2

2006 ◽  
Vol 70 (2) ◽  
pp. 348-354 ◽  
Author(s):  
Yasuhiko SUEZAWA ◽  
Isao KIMURA ◽  
Masako INOUE ◽  
Nana GOHDA ◽  
Motofumi SUZUKI
1999 ◽  
Vol 31 (5) ◽  
pp. 441-449 ◽  
Author(s):  
Arne Thell

AbstractPhylogenetic trees based on group I intron sequences and on internal transcribed spacer (ITS) sequences of mycobiont ribosomal genes were calculated and compared. Eight cetrarioid and four non-cetrarioid species of the Parmeliaceae were compared. The phylogeny based on group I intron sequences is partly congruent with the ITS sequence phylogeny. Group I intron sequences are presumably less informative for infragenic studies. The introns have a length of 214–233 nucleotides, and differ at up to 33% of the bases between species. All introns analysed are located between the positions 1516 and 1517 of the fungal 18S ribosomal RNA gene. Cetrarioid lichens form a non-homogeneous group within the Parmeliaceae according to both group I intron and ITS sequences.


Phytotaxa ◽  
2018 ◽  
Vol 371 (4) ◽  
pp. 241 ◽  
Author(s):  
FENGJIAN WANG ◽  
LIANGLIANG QI ◽  
XIANGYU ZHOU ◽  
YU LI

Two species of Xanthagaricus discovered in Hubei Province of China are described based on the macro- and micromorphological features. Their identity is further supported by molecular analysis of the internal transcribed spacer (ITS) of the ribosomal RNA gene. The phylogeny obtained here showed that one of the Chinese species is nested in an exclusive clade which in combination with its striking morphological features, infers that it represents a new species, named X. ianthinus, while the other species is placed as a member in the X. epipastus clade. Descriptions are accompanied with illustrations of macro- and micromorphological characters and a discussion of related taxa is presented.


2020 ◽  
pp. 089686082094477
Author(s):  
Tamonwan Chamroensakchai ◽  
Chadarat Kleebchaiyaphum ◽  
Sajja Tatiyanupanwong ◽  
Somchai Eiam-Ong ◽  
Talerngsak Kanjanabuch

We report the first case of peritoneal dialysis (PD) patients with peritonitis from Hortaea werneckii, a halotolerant black yeast-like fungus. The pathogen was confirmed by nucleotide sequences of internal transcribed spacer regions of the ribosomal RNA gene. A potential cause of this infection was tinea nigra on the patient’s palm. Therefore, gloving might be advised during PD bag exchange in patients or caregivers who have an obvious lesion of the palm.


Phytotaxa ◽  
2019 ◽  
Vol 420 (3) ◽  
pp. 233-240
Author(s):  
YI-FEI SUN ◽  
SHUN LIU ◽  
BAO-KAI CUI

A new species of Fistulina, F. tasmanica sp. nov., is described and illustrated from Tasmania in Australia. Fistulina tasmanica is distinguished by its pileate and fleshy basidiomata with rose pore surface, fibrous context, separated tubes with small pores (6–7 per mm), a monomitic hyphal system with only clamped generative hyphae, subellipsoid to ellipsoid basidiospores (5.4–6 × 3.4–4 μm) which are negative in Melzer’s reagent and cotton blue. Phylogenetic analyses inferred from the internal transcribed spacer regions (ITS) and the large subunit of nuclear ribosomal RNA gene (nLSU) sequences also confirmed that F. tasmanica is a new member within Fistulina.


MycoKeys ◽  
2020 ◽  
Vol 68 ◽  
pp. 115-135
Author(s):  
Rui Du ◽  
Fang Wu ◽  
Genevieve M. Gate ◽  
Yu-Cheng Dai ◽  
Xue-Mei Tian

Sidera is a polypore genus with white to cream or buff basidiomata, whose species in Hymenochaetales are poorly known. We study the phylogeny and diversity of Sidera based on our recent collections from tropic and subtropic Asian-Pacific regions. Phylogenetic analyses based on the internal transcribed spacer (ITS) and nuclear large subunit (nLSU) ribosomal RNA gene regions indicate that ten terminal lineages are well supported within Sidera. Based on morphological examination and phylogeny, four new species, viz. Sidera minutissima, S. parallela, S. srilankensis and S. tenuis are described, and a new combination, Sidera minutipora, is proposed. All these species are illustrated. Sidera minutissima is characterized by tiny basidiomata with bluish pores when fresh, generative hyphae dominating at the dissepiment edges, the presence of cystidioles, and allantoid basidiospores measuring 3.8–4.4 × 0.9–1.3 μm. Sidera parallela differs from other poroid species in the genus by having parallel tramal hyphae in combination with lunate basidiospores measuring 2.8–3.3 × 0.9–1.2 μm. Sidera srilankensis have generative and skeletal hyphae co-dominating at the dissepiment edges, and lunate basidiospores measuring 3.5–4 × 1–1.3 μm. Sidera tenuis is distinguished by small pores (8–10 per mm) and relatively long allantoid basidiospores measuring 4.2–5 × 0.8–1 μm. Sidera minutipora is characterized by buff to olivaceous buff basidiomata when dry, 5–7 pores per mm, rosette-like crystals rare, and allantoid basidiospores measuring 3.7–4.3 × 1–1.3 μm. An identification key to all accepted species is provided.


Nematology ◽  
2020 ◽  
Vol 22 (6) ◽  
pp. 713-722 ◽  
Author(s):  
Takuya Aikawa ◽  
Sota Ozawa ◽  
Noritoshi Maehara ◽  
Hayato Masuya ◽  
Katsunori Nakamura ◽  
...  

Summary To investigate the phoretic association between nematodes in the genus Bursaphelenchus and cerambycid beetles in north-eastern Japan, Pinus densiflora logs were placed in two pine forests in the towns of Yamada and Hiranai as beetle oviposition sites. At 1-2 years after oviposition, adults of Monochamus saltuarius emerged from the logs placed in Yamada and adults of Acalolepta sejuncta and A. fraudatrix emerged from the logs placed in Hiranai, and Bursaphelenchus nematodes were recovered from two of these species (M. saltuarius and A. sejuncta). Morphometrics and a phylogenetic analysis of the internal transcribed spacer regions 1 and 2 and the 5.8S ribosomal RNA gene indicated that both of these nematode populations were B. doui, which has previously only been known to use M. subfasciatus and A. fraudatrix as vectors. Therefore, this study demonstrated that this nematode utilises at least four species of beetles belonging to two genera as vectors in the field.


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