scholarly journals Morphological and molecular studies on Resinicium s. str.

2007 ◽  
Vol 85 (4) ◽  
pp. 420-436 ◽  
Author(s):  
Karen K. Nakasone
Keyword(s):  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Small Subunit ◽  
Caribbean Region ◽  
Sequence Analyses ◽  
Internal Transcribed Spacer Region ◽  
Resinicium Bicolor ◽  
Molecular Studies ◽  
The Caribbean

Resinicium Parmasto is typified by Resinicium bicolor (Alb. & Schwein.: Fr.) Parm., (Hymenochaetales, Basidiomycota), a readily recognized and widely distributed corticioid, lignicolous species in the northern hemisphere. Five new species of Resinicium closely allied to R. bicolor from the Caribbean region are described and illustrated: Resinicium confertum Nakasone, sp. nov., Resinicium monticola Nakasone, sp. nov., Resinicium mutabile Nakasone, sp. nov., Resinicium rimulosum Nakasone, sp. nov., and Resinicium tenue Nakasone, sp. nov. In addition, R. bicolor and Resinicium friabile Hjortstam & Melo are redescribed and illustrated. Sequence analyses of the internal transcribed spacer region support the recognition of the new taxa. Further, nuclear small subunit ribosomal RNA sequence data support the recognition of two groups of Resinicium species: the Resinicium s. str. group, including R. bicolor, and the Resinicium s.l. group that includes Resinicium furfuraceum (Bres.) Parm. The genus Resinicium is redefined and a key to the Resinicium s. str. species is provided.

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽  
2019 ◽  
Vol 409 (4) ◽  
pp. 189-201 ◽  
Author(s):  
MALKA SABA ◽  
DANNY HAELEWATERS ◽  
MUHAMMAD FIAZ ◽  
ABDUL NASIR KHALID ◽  
DONALD H. PFISTER
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Pine Forests ◽  
Evolutionary Relationships ◽  
Internal Transcribed Spacer Region ◽  
Bayesian Inferences ◽  
A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽  
2019 ◽  
Vol 401 (3) ◽  
pp. 199
Author(s):  
MALKA SABA ◽  
DANNY HAELEWATERS ◽  
MUHAMMAD FIAZ ◽  
ABDUL NASIR KHALID ◽  
DONALD H. PFISTER
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Pine Forests ◽  
Evolutionary Relationships ◽  
Internal Transcribed Spacer Region ◽  
Bayesian Inferences ◽  
A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

scholarly journals Studies on Wrightoporia from China 3. Wrightoporia subavellanea sp. nov. based on morphological characters and rDNA sequence data

Phytotaxa ◽  
2014 ◽  
Vol 175 (4) ◽  
pp. 225 ◽  
Author(s):  
JIA-JIA CHEN ◽  
Bao-Kai Cui
Keyword(s):  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Growth Habit ◽  
Sequence Data ◽  
Southern China ◽  
Molecular Study ◽  
Morphological Characters ◽  
Molecular Data ◽  
Internal Transcribed Spacer Region ◽  
Rdna Sequence Data

Wrightoporia subavellanea sp. nov. is described and illustrated from southern China on the basis of morphological characters and molecular data. It is characterized by an annual growth habit, resupinate basidiocarps with white rhizomorphs, large pores, narrow and strongly dextrinoid skeletal hyphae, broadly ellipsoid to subglobose, thick-walled, finely asperulate and strongly amyloid basidiospores (3.8–4.2 × 2.8–3.2 µm), and presence of gloeoplerous hyphae in trama. A molecular study based the combined ITS (internal transcribed spacer region) and nLSU (the large nuclear ribosomal RNA subunit) dataset supported the position of the new species in Wrightoporia. A key to accepted species of Wrightoporia in China is provided.

First report of Hericium cirrhatum from Pakistan

Mycotaxon ◽  
2020 ◽  
Vol 135 (4) ◽  
pp. 845-852
Author(s):  
Junaid Khan ◽  
Hassan Sher ◽  
Shah Hussain ◽  
Abdul Nasir Khalid
Keyword(s):  
Dna Sequence ◽  
Internal Transcribed Spacer ◽  
Morphological Characters ◽  
Sequence Analyses ◽  
Internal Transcribed Spacer Region ◽  
First Report ◽  
First Time

Hericium cirrhatum, a widespread but locally rare tooth fungus is reported for the first time from Pakistan. This species is characterized by white to cream semicircular basidiomata (usually arranged in tiers) with a granular to slightly spiny sterile upper surface, a fertile under surface with 10–15 mm long pointed spines, and ellipsoid to subglobose basidiospores. Morphological characters and DNA sequence analyses inferred from the nuclear ribosomal internal transcribed spacer region (nrITS) confirm the identity of the taxon.

Choiromyces cerebriformis (Tuberaceae), a new hypogeous species from Yunnan, China

Phytotaxa ◽  
2021 ◽  
Vol 482 (3) ◽  
pp. 251-260
Author(s):  
TIAN-JUN YUAN ◽  
OLIVIER RASPÉ ◽  
YONG-JUN LI ◽  
LI WANG ◽  
KAI-MEI SU ◽  
...  
Keyword(s):  
Related Species ◽  
Internal Transcribed Spacer ◽  
Yunnan Province ◽  
Genetic Distances ◽  
Morphological Evidence ◽  
Similar Species ◽  
Sequence Analyses ◽  
Internal Transcribed Spacer Region ◽  
Morphological Differences ◽  
Light Orange

A new hypogeous species, Choiromyces cerebriformis sp. nov. is described and illustrated from Yunnan province, China. Both morphological evidence and sequence analyses of the internal transcribed spacer region (ITS1-5.8S-ITS2) support the species as new to science. C. cerebriformis differs from other Choiromyces species in having ascomata with larger lobes and light orange-brown gleba, and globose ascospores with short stick-like sparse spines ornamentation. Morphological differences and genetic distances with the similar species C. helanshanensis and C. alveolatus are discussed. A phenotypic key including related species is provided.

scholarly journals An ITS-based phylogenetic framework for the genus Vorticella : finding the molecular and morphological gaps in a taxonomically difficult group

2013 ◽  
Vol 280 (1771) ◽  
pp. 20131177 ◽  
Author(s):  
Ping Sun ◽  
John C. Clamp ◽  
Dapeng Xu ◽  
Bangqin Huang ◽  
Mann Kyoon Shin ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
Small Subunit ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
Branching Order ◽  
Close Relationship ◽  
Molecular Phylogenetic ◽  
Phylogenetic Framework

Vorticella includes more than 100 currently recognized species and represents one of the most taxonomically challenging genera of ciliates. Molecular phylogenetic analysis of Vorticella has been performed so far with only sequences coding for small subunit ribosomal RNA (SSU rRNA); only a few of its species have been investigated using other genetic markers owing to a lack of similar sequences for comparison. Consequently, phylogenetic relationships within the genus remain unclear, and molecular discrimination between morphospecies is often difficult because most regions of the SSU rRNA gene are too highly conserved to be helpful. In this paper, we move molecular systematics for this group of ciliates to the infrageneric level by sequencing additional molecular markers—fast-evolving internal transcribed spacer (ITS) regions—in a broad sample of 66 individual samples of 28 morphospecies of Vorticella collected from Asia, North America and Europe. Our phylogenies all featured two strongly supported, highly divergent, paraphyletic clades (I, II) comprising the morphologically defined genus Vorticella . Three major lineages made up clade I, with a relatively well-resolved branching order in each one. The marked divergence of clade II from clade I confirms that the former should be recognized as a separate taxonomic unit as indicated by SSU rRNA phylogenies. We made the first attempt to elucidate relationships between species in clade II using both morphological and multi-gene approaches, and our data supported a close relationship between some morphospecies of Vorticella and Opisthonecta , indicating that relationships between species in the clade are far more complex than would be expected from their morphology. Different patterns of helix III of ITS2 secondary structure were clearly specific to clades and subclades of Vorticella and, therefore, may prove useful for resolving phylogenetic relationships in other groups of ciliates.

On the morphology and phylogeny of Robustodorus Andrássy, 2007 and two ‘Aphelenchoides’ species (Nematoda: Aphelenchoidinae)

Nematology ◽  
2018 ◽  
Vol 20 (7) ◽  
pp. 601-615
Author(s):  
Natsumi Kanzaki ◽  
Ebrahim Shokoohi ◽  
Hendrika Fourie ◽  
Antoinette Swart ◽  
Loureine Muller ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Small Subunit ◽  
Rrna Genes ◽  
Irrigation Scheme ◽  
Large Numbers ◽  
Molecular Studies ◽  
Garlic Bulb ◽  
Northern Cape ◽  
Production Areas

Summary In a survey of nematode damage in groundnut (Arachis hypogaea) (Fabaceae) production areas in the Vaalharts Irrigation Scheme in the Northern Cape Province of South Africa, a nematode was recovered and initially believed to be a member of the genus Robustodorus, i.e., the species has a characteristic robust stylet with very well-developed teardrop-shaped (garlic bulb-like) basal swellings. The specimens were recovered in large numbers from damaged hulls and kernels of field-collected groundnuts and, after thorough morphological and molecular studies, were subsequently identified as Aphelenchoides arachidis. Molecular analysis based on near-full-length small subunit (SSU), internal transcribed spacer (ITS) and D2-D3 expansion segments of the large subunit (LSU) rRNA genes supported the monophyly of A. arachidis, A. subtenuis and R. megadorus within the subfamily Aphelenchoidinae. Based on the typological characters observed in the present study compared with descriptions in the literature, these three species are considered as congeneric, i.e., they share very well-developed basal swellings of the stylet. Accordingly, these two Aphelenchoides species are transferred to Robustodorus as R. arachidis n. comb. and R. subtenuis n. comb. In addition, the male tail characters of Robustodorus and Aphelenchoides are discussed.

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