scholarly journals On the Artificial Germination of Pollen in Rice Plants. : V. Effect of low temperature on pollen germination at different stages of primordial panicle development.

1956 ◽  
Vol 25 (2) ◽  
pp. 70-70
Author(s):  
N. ENOMOTO ◽  
I. YAMADA ◽  
K. HOZUMI ◽  
T. KUSANAGI
Keyword(s):  
Low Temperature ◽  
Pollen Germination ◽  
Rice Plants ◽  
Panicle Development ◽  
Germination Of Pollen

Wheat Catalase Expressed in Transgenic Rice Plants Can Improve Tolerance Against Low Temperature Injury

2002 ◽  
pp. 277-287
Author(s):  
Takeshi Matsumuraa ◽  
Noriko Tabayashib ◽  
Yasuyo Kamagata ◽  
Chihiro Souma ◽  
Haruo Saruyama
Keyword(s):  
Low Temperature ◽  
Transgenic Rice ◽  
Rice Plants ◽  
Transgenic Rice Plants

Laboratory culture of the rice gall midge Pachydiplosis oryzae (Wood-Mason)

1969 ◽  
Vol 58 (3) ◽  
pp. 439-454 ◽  
Author(s):  
Nalini Perera ◽  
Henry E. Fernando
Keyword(s):  
Relative Humidity ◽  
Low Temperature ◽  
Gall Midge ◽  
Normal Activity ◽  
Laboratory Culture ◽  
Rice Plants ◽  
Rice Gall Midge ◽  
Mist Chamber ◽  
Gravid Females ◽  
Very High

No method has so far been available for the laboratory culture of Pachydiplosis oryzae Wood-Mason or for the controlled infestation of rice plants with this pest. Suitable techniques for the above have been developed and are described.For the laboratory culture of P. oryzae, exposure of rice seedlings to gravid females for oviposition and the subsequent maintenance of the plants at a very high humidity in a mist chamber gave high infestation rates but results were not consistent. But this method is suitable for the maintenance of a laboratory culture of this insect.A special oviposition tube was developed for inducing oviposition on limited areas of muslin, and this permitted easy observation, collection and handling of eggs.It was found that oviposition tended to occur most frequently between 1900 and 2200 hr. and that the midges most commonly laid 200–285 eggs.Eggs developed normally and hatched after seventy-two hours when the muslin carrying them was kept on barely moist filter paper in petri dishes.Sixty-hour-old eggs could be stored for up to five days at 80 ± 2°C. with the percentage hatch decreasing gradually from the first to the fifth day. Low temperature affected development and hatching of thirty-six-hour-old eggs more severely, and hatching was completely inhibited if eggs of this age were exposed to this low temperature for more than two days. Hatching was normal when eggs were maintained at relative humidities of and above 88.8 per cent., but below this level some eggs collapsed as a result of desiccation. At relative humidities below 94.8 per cent., larval movement was initially limited and thereafter inhibited and the larvae were contracted. Such larvae resumed normal activity on immersion in water. These immobilised larvae, obtained by exposure of fully developed eggs to a relative humidity in the range 92.9–94.8 per cent., could be revived by immersion in water and used for infesting paddy plants.Fully developed eggs, freshly hatched larvae and larvae subjected to relative humidities in the range 92.9–94.8 per cent. and subsequently revived, were transferred to rica plants bt spraying, or by pipetting on the leaf at the ligules or by pipetting on to the soil at the bases of plants. All these mathods proved successful but pipetting on to the soil between rows of plants was the simplest and is to be preferred for controlled infestation of plants.A very high relative humidity and wet plant surfaces as obtained in the mist chamber used in these studies were essential for successful infestation of rice plants by the gall midge larvae.

GERMINATION OF SWEET CHERRY (PRUNUS AVIUM L.) POLLEN IN VITRO AS INFLUENCED BY FUNGICIDES

1961 ◽  
Vol 41 (4) ◽  
pp. 740-743 ◽  
Author(s):  
G. W. Eaton
Keyword(s):  
Pollen Germination ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Pollen Tubes ◽  
Germination Of Pollen ◽  
Germination And Growth ◽  
Prunus Avium L

Effects of fungicidal concentrations of sulphur, dichlone, ferbam, and captan on sweet cherry (Prunus avium L.) pollen germination and growth were studied in artificial media. Sulphur did not reduce the germination of pollen. Dichlone and ferbam reduced germination from 53.0 per cent to 47.1 per cent and 40 per cent, respectively, reductions which were significant statistically. Sprayed at 0.2 pound/100 gallons or less, captan did not reduce germination. Captan, sprayed at 2 pounds/100 gallons, almost entirely prevented pollen germination, and arrested the elongation of pollen tubes.

scholarly journals Cytology and Taxonomic Description of Two Brachiarias (Congograss and Tannergrass)

1969 ◽  
Vol 54 ◽  
pp. 390-400
Author(s):  
A. Sotomayor-Ríos ◽  
S. C. Schank ◽  
R. Woodbury
Keyword(s):  
Pollen Germination ◽  
Pollen Viability ◽  
Tube Formation ◽  
Pollen Stainability ◽  
Reliable Test ◽  
Plant Introductions ◽  
Brachiaria Ruziziensis ◽  
Taxonomic Description ◽  
Germination Of Pollen

The chromosome number and microsporogenesis of two plant introductions, Congograss (Brachiaria ruziziensis) and Tannergrass (Brachiaria sp.), were determined to evaluate the possibility of utilizing these two grasses in a breeding program. Two techniques of pollen germination were utilized as a means to study pollen viability. Although germination on artificial media was successful, the in vivo technique provided a more reliable test of viability. Germination of pollen was examined in vivo with percent tube formation scored under both selfing and crossing. Both Tannergrass and Congograss were described taxonomically. The chromosome numbers of Congograss and Tannergrass were found to be 2n = 18 and 2n = 36 respectively. From observations made at metaphase I, meiosis appeared normal in Congograss and no laggards were observed. In Tannergrass, lagging univalents were observed which could account for the low pollen stainability obtained. The pollen germination on artificial media and the in vivo techniques undoubtedly can be applied to other genera.

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