Field Evaluation Against Aedes aegypti Larvae of Aluminum-Carboxymethylcellulose-Encapsulated Spore-Toxin Complex Formulation of Bacillus thuringiensis Serovar israelensis

2010 ◽  
Vol 103 (3) ◽  
pp. 570-576 ◽  
Author(s):  
O. Aguilar-Meza ◽  
M. Ramírez-Suero ◽  
J. S. Bernal ◽  
M. Ramírez-Lepe
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Field Evaluation ◽  
Complex Formulation ◽  
Toxin Complex

Synergisitic effect of chitinases and Bacillus thuringiensis israelensis spore-toxin complex against Aedes aegypti larvae

2011 ◽  
Vol 143 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Montserrat Ramírez-Suero ◽  
Gerardo Valerio-Alfaro ◽  
Julio S. Bernal ◽  
Mario Ramírez-Lepe
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Serratia Marcescens ◽  
Sole Carbon Source ◽  
Minimal Medium ◽  
Synergistic Effects ◽  
Streptomyces Griseus ◽  
Chitinolytic Activity ◽  
Bacillus Thuringiensis Israelensis ◽  
Toxin Complex

AbstractSix subspecies of Bacillus thuringiensis Berliner (Bt) were grown in minimal medium with chitin as the sole carbon source for 6 days to obtain Bt cell-free fermented broths, which were then evaluated for chitinolytic activity and tested against third-instar Aedes aegypti (L.) (Diptera: Culicidae) larvae. Bt pakistani showed the highest chitinolytic activity (approximately >2700 mU/mL), Bt kurstaki showed the lowest activity (approximately <2000 mU/mL), and Bt thompsoni, Bt aizawai, Bt israelensis, and Bt alesti showed intermediate activities (approximately 2100–2400 mU/mL). Bt aizawai and Bt thompsoni broths showed the highest toxicity (LC50) against third-instar A. aegypti larvae (approximately <290 mU/mL). Bt kurstaki broth showed the lowest toxicity (approximately 420 mU/mL), while Bt pakistani, Bt israelensis, and Bt alesti broths showed intermediate toxicities (approximately 360–460 mU/mL). A purified and biochemically characterized Bt aizawai chitinase and commercial chitinases (from Serratia marcescens Bizio and Streptomyces griseus Waksman and Henrici) were evaluated and compared for synergistic effects on Bt israelensis spore-toxin complex against third-instar A. aegypti larvae. The synergism factor value of Streptomyces griseus and Bt aizawai chitinases were >2 and approximately 1.4; synergism was not evident for the Serratia marcescens chitinase (synergism factor value approximately 0.9).

scholarly journals Single blinded semi-field evaluation of MAÏA® topical repellent ointment compared to unformulated 20% DEET against Anopheles gambiae, Anopheles arabiensis and Aedes aegypti in Tanzania

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Emmanuel Mbuba ◽  
Olukayode G. Odufuwa ◽  
Frank C. Tenywa ◽  
Rose Philipo ◽  
Mgeni M. Tambwe ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Anopheles Gambiae ◽  
Anopheles Arabiensis ◽  
Protective Efficacy ◽  
Latin Square ◽  
Field Evaluation ◽  
Sensu Stricto ◽  
Lower Limbs ◽  
Complete Protection ◽  
Regular User

Abstract Background N,N-Diethyl-3-methylbenzamide (DEET) topical mosquito repellents are effective personal protection tools. However, DEET-based repellents tend to have low consumer acceptability because they are cosmetically unappealing. More attractive formulations are needed to encourage regular user compliance. This study evaluated the protective efficacy and protection duration of a new topical repellent ointment containing 15% DEET, MAÏA® compared to 20% DEET in ethanol using malaria and dengue mosquito vectors in Bagamoyo Tanzania. Methods Fully balanced 3 × 3 Latin square design studies were conducted in large semi-field chambers using laboratory strains of Anopheles gambiae sensu stricto, Anopheles arabiensis and Aedes aegypti. Human volunteers applied either MAÏA® ointment, 20% DEET or ethanol to their lower limbs 6 h before the start of tests. Approximately 100 mosquitoes per strain per replicate were released inside each chamber, with 25 mosquitoes released at regular intervals during the collection period to maintain adequate biting pressure throughout the test. Volunteers recaptured mosquitoes landing on their lower limbs for 6 h over a period of 6 to 12-h post-application of repellents. Data analysis was conducted using mixed-effects logistic regression. Results The protective efficacy of MAÏA® and 20% DEET was not statistically different for each of the mosquito strains: 95.9% vs. 97.4% against An. gambiae (OR = 1.53 [95% CI 0.93–2.51] p = 0.091); 96.8% vs 97.2% against An. arabiensis (OR = 1.08 [95% CI 0.66–1.77] p = 0.757); 93.1% vs 94.6% against Ae. aegypti (OR = 0.76 [95% CI 0.20–2.80] p = 0.675). Average complete protection time (CPT) in minutes of MAÏA® and that of DEET was similar for each of the mosquito strains: 571.6 min (95% CI 558.3–584.8) vs 575.0 min (95% CI 562.1–587.9) against An. gambiae; 585.6 min (95% CI 571.4–599.8) vs 580.9 min (95% CI 571.1–590.7) against An. arabiensis; 444.1 min (95% CI 401.8–486.5) vs 436.9 min (95% CI 405.2–468.5) against Ae. aegypti. Conclusions MAÏA® repellent ointment provides complete protection for 9 h against both An. gambiae and An. arabiensis, and 7 h against Ae. aegypti similar to 20% DEET (in ethanol). MAÏA® repellent ointment can be recommended as a tool for prevention against outdoor biting mosquitoes in tropical locations where the majority of the people spend an ample time outdoor before going to bed.

Characterization of a novel Bacillus thuringiensis toxin active against Aedes aegypti larvae

Acta Tropica ◽  
2021 ◽  
pp. 106088
Author(s):  
Jiangyu Wu ◽  
Li Wei ◽  
Jiali He ◽  
Kang Fu ◽  
Xinxin Li ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Bacillus Thuringiensis Toxin

Field Evaluation of Indoor Thermal Fog and Ultra-Low Volume Applications For Control of Aedes aegypti in Thailand

2017 ◽  
Vol 33 (2) ◽  
pp. 116-127 ◽  
Author(s):  
A. Ponlawat ◽  
J. F. Harwood ◽  
J. L. Putnam ◽  
C. Nitatsukprasert ◽  
A. Pongsiri ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Field Evaluation ◽  
Low Volume

scholarly journals Evaluación de dos formulaciones de Bacillus thuringiensis H-14 para el control de larvas de Aedes aegypti

Biomédica ◽  
1987 ◽  
Vol 7 (1-2) ◽  
pp. 5 ◽  
Author(s):  
Marco F. Suárez ◽  
Dwight Ayala ◽  
Michael J. Nelson
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti

scholarly journals Cloning and Expression of Two Crystal Protein Genes, cry30Ba1 and cry44Aa1, Obtained from a Highly Mosquitocidal Strain, Bacillus thuringiensis subsp. entomocidus INA288

2006 ◽  
Vol 72 (8) ◽  
pp. 5673-5676 ◽  
Author(s):  
Takeshi Ito ◽  
Tomonori Ikeya ◽  
Ken Sahara ◽  
Hisanori Bando ◽  
Shin-ichiro Asano
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Culex Pipiens ◽  
Crystal Protein ◽  
Cloning And Expression ◽  
Culex Pipiens Pallens ◽  
Bacillus Thuringiensis Subsp ◽  
Pipiens Pallens ◽  
Crystal Protein Genes

ABSTRACT Two novel crystal protein genes, cry30Ba and cry44Aa, were cloned from Bacillus thuringiensis subsp. entomocidus INA288 and expressed in an acrystalliferous strain. Cry44Aa crystals were highly toxic to second-instar Culex pipiens pallens (50% mortality concentration [LC50] = 6 ng/ml) and Aedes aegypti (LC50 = 12 ng/ml); however, Cry30Ba crystals were not toxic.

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