scholarly journals Celiac disease screening in Brazilian patients with osteoporosis

2014 ◽  
Vol 58 (3) ◽  
pp. 270-273 ◽  
Author(s):  
Luiza Gusso ◽  
Mariana Cionek Simões ◽  
Thelma L. Skare ◽  
Renato Nisihara ◽  
Claudine C. Burkiewicz ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Indirect Immunofluorescence ◽  
Gastrointestinal Endoscopy ◽  
Tissue Transglutaminase ◽  
Geographic Region ◽  
Duodenal Biopsy ◽  
Elisa Assay ◽  
Immunofluorescence Method ◽  
Endomysial Antibodies ◽  
Low Prevalence

Objective : To analyze if it is worthwhile to screen Brazilian osteoporotic patients for celiac disease (CD).Subjects and methods : One hundred patients with osteoporosis and 97 controls were evaluated for IgA-EmA (IgA anti-endomysial antibodies) by indirect immunofluorescence method and IgG-anti-tTG (tissue transglutaminase) by ELISA assay. Positive patients were invited to have gastrointestinal endoscopy with jejunal biopsy.Results : Two patients had positive IgG-anti-tTG test and one of them also showed positive IgA-EmA. Only the latter had a positive duodenal biopsy for CD. None of the controls were positive for either auto-antibodies.Conclusion : We observed low prevalence of CD in osteoporotic Brazilian patients. This finding does not support routine screening for CD in patients with osteoporosis in our geographic region. Arq Bras Endocrinol Metab. 2014;58(3):270-3

scholarly journals Atypical presentations of celiac disease

2016 ◽  
Vol 22 (3) ◽  
pp. 181-185
Author(s):  
Adriana Luminita Balasa ◽  
Cristina Maria Mihai ◽  
Tatiana Chisnoiu ◽  
Corina Elena Frecus
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Screening Method ◽  
Total Serum ◽  
Genetic Syndromes ◽  
Gliadin Antibodies ◽  
Pediatric Clinic ◽  
Endomysial Antibodies ◽  
Atypical Presentations ◽  
Celiac Disease Antibodies

Abstract In this study we evaluated the association of celiac disease in 81 children with autoimmune disease and genetic syndromes over a two years periods (January 2014 to July 2016) in Pediatric Clinic in Constanta. Because the extraintestinal symptoms are an atypical presentation of celiac disease we determined in these children the presence of celiac disease antibodies: Anti-tissue Transglutaminase Antibody IgA and IgA total serum level as a screening method followeds in selective cases by Anti-tissue Transglutaminase Antibody IgG, anti-endomysial antibodies, deamidated gliadin antibodies IgA and IgG and intestinal biopsia. In our study 8 patients had been diagnosed with celiac disease with extraintestinal symptoms, of which 4 with type 1 diabetes, 1 patient with ataxia, 2 patients with dermatitis herpetiformis and 1 patient with Down syndrome that associate also autoimmune thyroiditis, alopecia areata, enamel hypoplasia.

scholarly journals Celiac Disease After Administration of Immune Checkpoint Inhibitors: A Case Report

2021 ◽  
Vol 12 ◽  
Author(s):  
Julie Leblanc ◽  
Solene Hoibian ◽  
Agathe Boucraut ◽  
Jean-Philippe Ratone ◽  
Louis Stoffaes ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Immune Checkpoint ◽  
Immune Checkpoint Inhibitors ◽  
Gastrointestinal Endoscopy ◽  
Checkpoint Inhibitors ◽  
Villous Atrophy ◽  
Tissue Transglutaminase ◽  
Gluten Free ◽  
Serum Iga ◽  
Iga Antibodies

Immune checkpoint inhibitors (ICI) reinvigorate the immune system to recognize and destroy tumor cells. Because of this biological mechanism, patients might develop autoimmune toxicities, notably in the digestive tract (most frequently, hepatitis or colitis). A 70-year-old man with relapsed mesothelioma was treated with nivolumab in 3rd line. He was hospitalized for watery and foul-smelling diarrhea. He underwent gastrointestinal endoscopy, showing duodenitis and villous atrophy and measurement of serum IgA antibodies to tissue transglutaminase (tTG-IgA+), leading to the diagnosis of ICI-induced celiac disease. He was treated with steroids, proton pump inhibitors, and a gluten-free diet. If ICI-induced celiac disease is rare in the literature, increasing reports suggest that celiac disease might represent an underestimated ICI toxicity. This case highlights the necessity of complementary investigation (including tTG-IgA and endoscopic biopsies) in patients with atypical digestive symptoms during immunotherapy.

Immunoassays for the detection of IgA antibodies to tissue transglutaminase: significance of multiples of the upper limit of normal and inter-assay correlations

2016 ◽  
Vol 54 (2) ◽  
Author(s):  
Brenda B. Suh-Lailam ◽  
K. Wayne Davis ◽  
Anne E. Tebo
Keyword(s):  
Celiac Disease ◽  
Indirect Immunofluorescence ◽  
Tissue Transglutaminase ◽  
Immunofluorescence Assay ◽  
Indirect Immunofluorescence Assay ◽  
Healthy Individuals ◽  
Reference Test ◽  
Upper Limit ◽  
Iga Antibodies ◽  
Diagnostic Pathways

AbstractThe presence of IgA antibodies to tissue transglutaminase (anti-tTg) is associated with variable risk for celiac disease. The use of common multiples of the upper limit of normal (ULN) has been suggested to optimize diagnostic pathways as well as improve harmonization between assays.The characteristics of four anti-tTG IgA assays relative to endomysial IgA (EMA) by indirect immunofluorescence assay (IFA) as reference test were assessed. Commutability between anti-tTG immunoassays and/or EMA based on manufacturer’s recommended cut-off values and three common multiples of ULN (3×, 5× and 10×) was also investigated. Sera from 200 patients and 100 healthy individuals were analyzed.At manufacturer’s cut-off; the sensitivities for the tTG assays ranged from 72.5% to 98.6% and specificities from 60.3% to 99.2%. The percent positive agreements between any anti-tTG and EMA or any two anti-tTG immunoassays varied from 56.7% to 98.0% and 46.7% to 100.0%, respectively. At 3×, 5× or 10× ULNs, the inter-rater reliability as measured by Cohen κ between any two anti-tTG assays were quite variable and ranged from 0.28 to 0.96, 0.26 to 0.89 or 0.13 to 0.78, respectively. Furthermore, the percent positive agreements between any two anti-tTg IgA immunoassays ranged from 83.1% to 98.2%, 92.0% to 100%, or 100%, at 3×, 5× or 10×, respectively.Commutability between tTG IgA immunoassays or tTG IgA and EMA is kit-dependent and common multiples of the ULN are not sufficient to correct for inter-assay variations. Many factors influence the performance of anti-tTG IgA assays which limit their commutability.

Screening for Autoantibodies to Tissue Transglutaminase Reveals a Low Prevalence of Celiac Disease in Blood Donors with Cryptogenic Hypertransaminasemia

Digestion ◽  
2001 ◽  
Vol 64 (2) ◽  
pp. 87-91 ◽  
Author(s):  
M. Soresi ◽  
M. Amplo ◽  
R. Agliastro ◽  
R. Sesti ◽  
G. Di Giovanni ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Blood Donors ◽  
Tissue Transglutaminase ◽  
Low Prevalence

scholarly journals Frequency of celiac disease in patients with vitiligo

2020 ◽  
Vol 7 (10) ◽  
pp. 1475
Author(s):  
Cenk Sunu ◽  
Ceyhun Varim ◽  
Cengiz Karacaer ◽  
Yasin Kalpakci
Keyword(s):  
Body Mass Index ◽  
Celiac Disease ◽  
Essential Role ◽  
Tissue Transglutaminase ◽  
Serum Levels ◽  
Duodenal Biopsy ◽  
Control Group ◽  
Study Group ◽  
Igg Antibodies ◽  
Significant Difference

Background: Celiac disease (CD) is an autoimmune and inflammatory disease that occurs in the small intestine as a result of gluten intake in individuals. Vitiligo, in which autoimmune factors play an essential role, is associated with depigmentation due to the loss of epidermal melanocytes. Many autoimmune diseases are known to be associated with vitiligo. This study aims to determine the frequency of CD in vitiligo cases.Methods: Out of 61 vitiligo patients, 32 (52.4%) are women, and 29 (47.6%) are men; of the 119 healthy volunteers, 58 (48.7%) are women, and 61 (51.3%) are men. Serum levels of tissue transglutaminase (tTG) IgA and tTG IgG antibodies were measured in all participants. If at least one of these two antibodies are positive, mucosal biopsies were taken from the second section of the duodenum by endoscopy.Results: There is no significant difference between the study group and the control group in terms of age and body mass index (BMI) (p=0.16, p=0.80, respectively). tTG IgA and tTG IgG were positive in one patient in each group. In both patients, the results of the duodenal biopsy were histopathological compatible with CD. There was no difference between the vitiligo group and the control group in terms of CD frequency (p=0.56).Conclusion: The frequency of CD in vitiligo patients is similar to the control group. However, it should be kept in mind that the frequency of CD in patients with vitiligo may be higher than the rates assumed incidental, and necessary research should be carried out for early diagnosis in such patients.

scholarly journals Tissue Transglutaminase Antibody and Its Association with Duodenal Biopsy in Diagnosis of Pediatric Celiac Disease

2019 ◽  
Vol 22 (4) ◽  
pp. 350 ◽  
Author(s):  
Daleep K. Meena ◽  
Shalini Akunuri ◽  
Preetam Meena ◽  
Ashok Bhramer ◽  
Shiv D. Sharma ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Duodenal Biopsy

Combining antibody tests and taking into account antibody levels improves serologic diagnosis of celiac disease

2015 ◽  
Vol 53 (10) ◽  
Author(s):  
Matthijs Oyaert ◽  
Pieter Vermeersch ◽  
Gert De Hertogh ◽  
Martin Hiele ◽  
Nathalie Vandeputte ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Human Leukocyte ◽  
Duodenal Biopsy ◽  
Hla Typing ◽  
High Antibody ◽  
Antibody Testing ◽  
Likelihood Ratios ◽  
Serologic Diagnosis ◽  
Antibody Levels

AbstractThe European Society for Pediatric Gastroenterology and Nutrition states that if IgA anti-tissue transglutaminase (tTG) exceeds 10 times the upper limit of normal (ULN), there is the possibility to diagnose celiac disease (CD) without duodenal biopsy, if supported by anti-endomysium testing and human leukocyte antigen (HLA) typing. We aimed to evaluate whether combining IgA tTG and IgG anti-deamidated gliadin peptide (DGP) antibody testing and taking into account the antibody levels improves clinical interpretation.We calculated likelihood ratios for various test result combinations using data obtained from newly diagnosed CD patients (n=156) [13 children <2 years, 45 children between 2 and 16 years, and 98 adults (>16 years)] and 974 disease controls. All patients and controls underwent duodenal biopsy. IgA anti-tTG and IgG anti-DGP assays were from Thermo Fisher and Inova.Likelihood ratios for CD markedly increased with double positivity and increasing antibody levels of IgA anti-tTG and IgG anti-DGP. Patients with double positivity and high antibody levels (>3 times, >10 times ULN) had a high probability for having CD (likelihood ratio ≥649 for >3 times ULN and ∞ for >10 times ULN). The fraction of CD patients with double positivity and high antibody levels was 59%–67% (depending on the assay) for >3 ULN and 33%–36% (depending on the assay) for >10 ULN, respectively. This fraction was significantly higher in children with CD than in adults.Combining IgG anti-DGP with IgA anti-tTG and defining thresholds for antibody levels improves the serologic diagnosis of CD.

scholarly journals Prevalence of Celiac Disease and Celiac Autoimmunity in the Toba Native Amerindian Community of Argentina

2015 ◽  
Vol 29 (8) ◽  
pp. 431-434 ◽  
Author(s):  
Horacio Vázquez ◽  
María de la Paz Temprano ◽  
Emilia Sugai ◽  
Stella M Scacchi ◽  
Cecilia Souza ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Human Leukocyte ◽  
Screen Test ◽  
Leukocyte Antigen ◽  
Day Range ◽  
Gliadin Peptides ◽  
Endomysial Antibodies ◽  
Very High ◽  
Hla Dq2

BACKGROUND: Celiac disease (CD) is mostly recognized among subjects with a Caucasian ethnic ancestry. No studies have explored conditions predisposing Amerindians to CD.OBJECTIVE: To prospectively assess environmental, genetic and serological conditions associated with CD among members of the Toba native population attending a multidisciplinary sanitary mission.METHODS: An expert nutritionist determined daily gluten intake using an established questionnaire. Gene typing for the human leukocyte antigen (HLA) class II alleles was performed on DNA extracted from peripheral blood (HLA DQ2/DQ8 haplotype). Serum antibodies were immunoglobulin (Ig) A tissue transglutaminase (tTG) and the composite deamidated gliadin peptides/tTG Screen test. Positive cases were tested for IgA endomysial antibodies.RESULTS: A total of 144 subjects (55% female) were screened. The estimated mean gluten consumption was 43 g/day (range 3 g/day to 185 g/day). Genetic typing showed that 73 of 144 (50.7%) subjects had alleles associated with CD; 69 (94.5%) of these subjects had alleles for HLA DQ8 and four had DQ2 (5.5%). Four and six subjects had antibody concentrations above the cut-off established by the authors’ laboratory (>3 times the upper limit of normal) for IgA tTG and deamidated gliadin peptides/tTG screen, respectively. Four of these had concomitant positivity for both assays and endomysial antibodies were positive in three subjects who also presented a predisposing haplotype.CONCLUSION: The present study was the first to detect CD in Amerindians. The native Toba ethnic population has very high daily gluten consumption and a predisposing genetic background. We detected subjects with persistent CD autoimmunity and, at least, three of them fulfilled serological criteria for CD diagnosis.

Positive tissue transglutaminase antibodies with negative endomysial antibodies: Unresolved issues in diagnosing celiac disease

2020 ◽  
pp. 112910
Author(s):  
Maria Infantino ◽  
Mario Merone ◽  
Mariangela Manfredi ◽  
Valentina Grossi ◽  
Alessandra Landini ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Tissue Transglutaminase Antibodies ◽  
Endomysial Antibodies
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