scholarly journals Association of Anti-tissue Transglutaminase Antibody Titers and Duodenal Biopsy Findings in Pediatric Patients of Celiac Disease

Cureus ◽  
2021 ◽  
Author(s):  
Kanchan Taneja ◽  
Nidhi Mahajan ◽  
Anuradha Rai ◽  
Sonali Malik ◽  
Arti Khatri
Keyword(s):  
Celiac Disease ◽  
Pediatric Patients ◽  
Tissue Transglutaminase ◽  
Duodenal Biopsy ◽  
Antibody Titers

scholarly journals Reliability of the Multiplex CytoBead CeliAK Immunoassay to Assess Anti-tTG IgA for Celiac Disease Screening

2021 ◽  
Vol 8 ◽  
Author(s):  
Diyora Abdukhakimova ◽  
Kuanysh Dossybayeva ◽  
Anna Grechka ◽  
Zhaina Almukhamedova ◽  
Alyona Boltanova ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Pediatric Patients ◽  
Tissue Transglutaminase ◽  
Multiplex Assay ◽  
Single Parameter ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Markers ◽  
Serum Samples ◽  
Conventional Elisa ◽  
Iga Antibody

Background and Objective: The diagnosis of Celiac Disease (CD) is first based on the positivity for specific serological markers. The CytoBead CeliAK immunoassay simultaneously measures antibodies (IgA) directed to tissue transglutaminase (tTG), endomysium (EMA), and deamidated gliadin (DG), in addition to providing a control for total IgA levels. The aim of this study is to assess the reliability of this multiplex assay to detect anti-tTG IgA positive patients, compared with a conventional single-parameter enzyme-linked immunosorbent assay (ELISA).Methods: Serum samples from 149 pediatric patients were assessed by both CytoBead CeliAK immunoassay and ELISA, in order to evaluate their concordance for the measurement of anti-tTG IgA.Results: The measurement of anti-tTG IgA by CytoBead CeliAK immunoassay basically showed a complete concordance rate with the conventional and single-parameter ELISA, according to the respective cutoff values (3 U/ml and 10 U/ml).Conclusions: Our comparative analysis demonstrates a substantial equivalency between multiplex CytoBead CeliAK assay and the single-parameter conventional ELISA to assess anti-tTG IgA antibody in the context of the screening for CD in children. Importantly, CytoBead CeliAK assay could present some preanalytic, analytic, and economic advantages.

scholarly journals Frequency of celiac disease in patients with vitiligo

2020 ◽  
Vol 7 (10) ◽  
pp. 1475
Author(s):  
Cenk Sunu ◽  
Ceyhun Varim ◽  
Cengiz Karacaer ◽  
Yasin Kalpakci
Keyword(s):  
Body Mass Index ◽  
Celiac Disease ◽  
Essential Role ◽  
Tissue Transglutaminase ◽  
Serum Levels ◽  
Duodenal Biopsy ◽  
Control Group ◽  
Study Group ◽  
Igg Antibodies ◽  
Significant Difference

Background: Celiac disease (CD) is an autoimmune and inflammatory disease that occurs in the small intestine as a result of gluten intake in individuals. Vitiligo, in which autoimmune factors play an essential role, is associated with depigmentation due to the loss of epidermal melanocytes. Many autoimmune diseases are known to be associated with vitiligo. This study aims to determine the frequency of CD in vitiligo cases.Methods: Out of 61 vitiligo patients, 32 (52.4%) are women, and 29 (47.6%) are men; of the 119 healthy volunteers, 58 (48.7%) are women, and 61 (51.3%) are men. Serum levels of tissue transglutaminase (tTG) IgA and tTG IgG antibodies were measured in all participants. If at least one of these two antibodies are positive, mucosal biopsies were taken from the second section of the duodenum by endoscopy.Results: There is no significant difference between the study group and the control group in terms of age and body mass index (BMI) (p=0.16, p=0.80, respectively). tTG IgA and tTG IgG were positive in one patient in each group. In both patients, the results of the duodenal biopsy were histopathological compatible with CD. There was no difference between the vitiligo group and the control group in terms of CD frequency (p=0.56).Conclusion: The frequency of CD in vitiligo patients is similar to the control group. However, it should be kept in mind that the frequency of CD in patients with vitiligo may be higher than the rates assumed incidental, and necessary research should be carried out for early diagnosis in such patients.

scholarly journals Tissue Transglutaminase Antibody and Its Association with Duodenal Biopsy in Diagnosis of Pediatric Celiac Disease

2019 ◽  
Vol 22 (4) ◽  
pp. 350 ◽  
Author(s):  
Daleep K. Meena ◽  
Shalini Akunuri ◽  
Preetam Meena ◽  
Ashok Bhramer ◽  
Shiv D. Sharma ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Duodenal Biopsy

Combining antibody tests and taking into account antibody levels improves serologic diagnosis of celiac disease

2015 ◽  
Vol 53 (10) ◽  
Author(s):  
Matthijs Oyaert ◽  
Pieter Vermeersch ◽  
Gert De Hertogh ◽  
Martin Hiele ◽  
Nathalie Vandeputte ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Human Leukocyte ◽  
Duodenal Biopsy ◽  
Hla Typing ◽  
High Antibody ◽  
Antibody Testing ◽  
Likelihood Ratios ◽  
Serologic Diagnosis ◽  
Antibody Levels

AbstractThe European Society for Pediatric Gastroenterology and Nutrition states that if IgA anti-tissue transglutaminase (tTG) exceeds 10 times the upper limit of normal (ULN), there is the possibility to diagnose celiac disease (CD) without duodenal biopsy, if supported by anti-endomysium testing and human leukocyte antigen (HLA) typing. We aimed to evaluate whether combining IgA tTG and IgG anti-deamidated gliadin peptide (DGP) antibody testing and taking into account the antibody levels improves clinical interpretation.We calculated likelihood ratios for various test result combinations using data obtained from newly diagnosed CD patients (n=156) [13 children <2 years, 45 children between 2 and 16 years, and 98 adults (>16 years)] and 974 disease controls. All patients and controls underwent duodenal biopsy. IgA anti-tTG and IgG anti-DGP assays were from Thermo Fisher and Inova.Likelihood ratios for CD markedly increased with double positivity and increasing antibody levels of IgA anti-tTG and IgG anti-DGP. Patients with double positivity and high antibody levels (>3 times, >10 times ULN) had a high probability for having CD (likelihood ratio ≥649 for >3 times ULN and ∞ for >10 times ULN). The fraction of CD patients with double positivity and high antibody levels was 59%–67% (depending on the assay) for >3 ULN and 33%–36% (depending on the assay) for >10 ULN, respectively. This fraction was significantly higher in children with CD than in adults.Combining IgG anti-DGP with IgA anti-tTG and defining thresholds for antibody levels improves the serologic diagnosis of CD.

scholarly journals Celiac disease screening in Brazilian patients with osteoporosis

2014 ◽  
Vol 58 (3) ◽  
pp. 270-273 ◽  
Author(s):  
Luiza Gusso ◽  
Mariana Cionek Simões ◽  
Thelma L. Skare ◽  
Renato Nisihara ◽  
Claudine C. Burkiewicz ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Indirect Immunofluorescence ◽  
Gastrointestinal Endoscopy ◽  
Tissue Transglutaminase ◽  
Geographic Region ◽  
Duodenal Biopsy ◽  
Elisa Assay ◽  
Immunofluorescence Method ◽  
Endomysial Antibodies ◽  
Low Prevalence

Objective : To analyze if it is worthwhile to screen Brazilian osteoporotic patients for celiac disease (CD).Subjects and methods : One hundred patients with osteoporosis and 97 controls were evaluated for IgA-EmA (IgA anti-endomysial antibodies) by indirect immunofluorescence method and IgG-anti-tTG (tissue transglutaminase) by ELISA assay. Positive patients were invited to have gastrointestinal endoscopy with jejunal biopsy.Results : Two patients had positive IgG-anti-tTG test and one of them also showed positive IgA-EmA. Only the latter had a positive duodenal biopsy for CD. None of the controls were positive for either auto-antibodies.Conclusion : We observed low prevalence of CD in osteoporotic Brazilian patients. This finding does not support routine screening for CD in patients with osteoporosis in our geographic region. Arq Bras Endocrinol Metab. 2014;58(3):270-3

scholarly journals Celiac Disease and Immunoglobulin A Deficiency: How Effective Are the Serological Methods of Diagnosis?

2002 ◽  
Vol 9 (6) ◽  
pp. 1295-1300 ◽  
Author(s):  
V. Kumar ◽  
M. Jarzabek-Chorzelska ◽  
J. Sulej ◽  
Krystyna Karnewska ◽  
T. Farrell ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Pediatric Patients ◽  
Tissue Transglutaminase ◽  
False Negative ◽  
Immunoglobulin A ◽  
Iga Deficiency ◽  
Igg Antibodies ◽  
Serological Tests ◽  
Asymptomatic Patients ◽  
Antibody Tests

ABSTRACT Immunoglobulin A (IgA) deficiency is 10 to 15 times more common in patients with celiac disease (CD) than in healthy subjects. Serological tests have become the preferred methods of diagnosing CD in both symptomatic and asymptomatic patients. However, commercially available serological methods are limited in that they detect only the IgA isotype of antibodies (with the exception of IgG gliadin assays); hence, IgA-deficient patients with CD may yield false-negative serology. Fifteen pediatric patients with CD and 10 IgA-deficient pediatric patients without CD were examined for IgA and IgG antibodies to endomysium, gliadin, and tissue transglutaminase. Twenty-five specimens from patients with IgA deficiency were examined. Fifteen were from patients with CD, and 10 were patients without CD. All 15 IgA-deficient patients with CD were positive for endomysium antibodies of the IgG isotype and for IgG gliadin antibodies. All but one of the IgA-deficient patients with CD were also positive for IgG tissue transglutaminase antibodies. None of the IgA-deficient patients without CD were positive for any of the antibody markers. All the specimens examined were also negative for IgA-specific antibodies to endomysium, gliadin, and tissue transglutaminase. IgG-specific antibody tests for endomysium, gliadin, and tissue transglutaminase are useful for the identification of IgA-deficient patients with CD. IgG antibody tests along with tests routinely being used in clinical laboratories can reliably detect all active patients with CD. In addition, the levels of these CD-specific IgG antibodies could be used to monitor patient dietary compliance.

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