Screening for Autoantibodies to Tissue Transglutaminase Reveals a Low Prevalence of Celiac Disease in Blood Donors with Cryptogenic Hypertransaminasemia

Digestion ◽  
2001 ◽  
Vol 64 (2) ◽  
pp. 87-91 ◽  
Author(s):  
M. Soresi ◽  
M. Amplo ◽  
R. Agliastro ◽  
R. Sesti ◽  
G. Di Giovanni ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Blood Donors ◽  
Tissue Transglutaminase ◽  
Low Prevalence

scholarly journals IgG Antibodies against Deamidated Gliadin Peptides for Diagnosis of Celiac Disease in Patients with IgA Deficiency

2010 ◽  
Vol 56 (3) ◽  
pp. 464-468 ◽  
Author(s):  
Danilo Villalta ◽  
Elio Tonutti ◽  
Christian Prause ◽  
Sibylle Koletzko ◽  
H Holm Uhlig ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Blood Donors ◽  
Tissue Transglutaminase ◽  
Iga Deficiency ◽  
Diagnostic Sensitivity ◽  
Igg Antibodies ◽  
Diagnostic Specificity ◽  
Gliadin Peptides ◽  
Endomysium Antibodies ◽  
Monkey Esophagus

AbstractBackground: Assays for IgG antibodies against deamidated gliadin (IgG-anti-dGli) are comparable in performance with tests detecting IgA antibodies against tissue transglutaminase (IgA-anti-tTG) in diagnosing celiac disease (CD). IgA-anti-tTG are absent in IgA deficiency, a condition often associated with CD. In IgA deficiency, IgG-anti-tTG, which have a lower overall diagnostic accuracy, are routinely measured. We examined whether IgG-anti-dGli would be useful for diagnosing CD in patients with IgA deficiency.Methods: We studied 34 IgA-deficient CD patients, 185 IgA-competent newly diagnosed children with CD, 316 children without CD, 400 adult blood donors, and 6 control IgA-deficient individuals without CD. Anti-dGli and anti-tTG were measured by ELISA, and endomysium antibodies (EmA) were measured by immunofluorescence on monkey esophagus (IgA as well as IgG class for all antibodies). We calculated diagnostic sensitivity (percentage of patients above cutoff with 95% CIs) according to age-specific cutoffs for 95% diagnostic specificity and according to cutoffs proposed by the manufacturer of the assays.Results: No IgA-deficient CD patients were positive for any IgA-based antibody assay. Diagnostic sensitivity of IgG-anti-tTG was 91.2% (95% CI 76.3%–97.7%) according to age-specific cutoffs and 82.4% (66.1%–92.0%) according to manufacturer cutoffs. The diagnostic sensitivity of IgG-EmA was 75.8% (58.8%–87.4%) and the sensitivity of IgG-anti-dGli was 88.2% (72.8%–95.9%) according to both cutoffs.Conclusions: IgG-anti-dGli and IgG-anti-tTG have comparable diagnostic sensitivities for IgA-deficient celiac patients. IgG-anti-dGli may be useful for diagnosing CD in IgA-deficient patients.

scholarly journals Celiac disease screening in Brazilian patients with osteoporosis

2014 ◽  
Vol 58 (3) ◽  
pp. 270-273 ◽  
Author(s):  
Luiza Gusso ◽  
Mariana Cionek Simões ◽  
Thelma L. Skare ◽  
Renato Nisihara ◽  
Claudine C. Burkiewicz ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Indirect Immunofluorescence ◽  
Gastrointestinal Endoscopy ◽  
Tissue Transglutaminase ◽  
Geographic Region ◽  
Duodenal Biopsy ◽  
Elisa Assay ◽  
Immunofluorescence Method ◽  
Endomysial Antibodies ◽  
Low Prevalence

Objective : To analyze if it is worthwhile to screen Brazilian osteoporotic patients for celiac disease (CD).Subjects and methods : One hundred patients with osteoporosis and 97 controls were evaluated for IgA-EmA (IgA anti-endomysial antibodies) by indirect immunofluorescence method and IgG-anti-tTG (tissue transglutaminase) by ELISA assay. Positive patients were invited to have gastrointestinal endoscopy with jejunal biopsy.Results : Two patients had positive IgG-anti-tTG test and one of them also showed positive IgA-EmA. Only the latter had a positive duodenal biopsy for CD. None of the controls were positive for either auto-antibodies.Conclusion : We observed low prevalence of CD in osteoporotic Brazilian patients. This finding does not support routine screening for CD in patients with osteoporosis in our geographic region. Arq Bras Endocrinol Metab. 2014;58(3):270-3

scholarly journals PREVALENCE OF GENETIC SUSCEPTIBILITY FOR CELIAC DISEASE IN BLOOD DONORS IN SÃO PAULO, BRAZIL

2016 ◽  
Vol 53 (4) ◽  
pp. 267-272 ◽  
Author(s):  
Janaína Guilhem MUNIZ ◽  
Vera Lucia SDEPANIAN ◽  
Ulysses FAGUNDES NETO
Keyword(s):  
Celiac Disease ◽  
Genetic Markers ◽  
Blood Donors ◽  
Tissue Transglutaminase ◽  
Sao Paulo ◽  
São Paulo ◽  
Informed Consent Form ◽  
High Prevalence ◽  
Antibody Class ◽  
Hla Dq2

ABSTRACT Background Celiac disease is a permanent intolerance induced by gluten, which is expressed by T-cell mediated enteropathy, and has a high prevalence in the general population. There is evidence of a strong genetic predisposition to celiac disease. Objective To determine the prevalence of genetic markers HLA-DQ2 and HLA-DQ8 in blood donors from São Paulo and measure human recombinant tissue transglutaminase antibody IgA class in HLA-DQ2 and HLA-DQ8 positive donors. Methods A total of 404 blood donors from São Paulo city and Jundiaí were included in the study and signed the informed consent form. Information regarding diarrhea, constipation and abdominal pain in the last 3 months was collected. Determination of HLADQ2 and HLADQ8 alleles was performed in all participants and human recombinant tissue transglutaminase antibody class IgA was measured only in blood donors who presentedDQ2 and/or DQ8. Results HLADQ2 and/or HLADQ8 were positive in 49% (198/404) of subjects. Positive samples were associated with alleles DR3, DR4, DR7, DR11 and DR12. The most frequent genotype was DR4-DQ8, which was present in 13.6% of samples, followed by genotypes DR3-DQ2 and DR7-DQ2 with DQB1*02 in heterozygous, which were present in 10.4% and 8.7%, respectively. Eleven out of 198 positive donors (5%) were positive to human tissue transglutaminase test. Conclusion We observed a high prevalence of genetic markers for celiac disease, HLA-DQ2 and HLA-DQ8, in blood donors from São Paulo, similar to prevalence described in Europe. These findings show that the prevalence of celiac disease should not be rare in our country, but underdiagnosed.

Screening of Tissue Transglutaminase Antibody in Healthy Blood Donors for Celiac Disease Screening in the Turkish Population

2004 ◽  
Vol 49 (9) ◽  
pp. 1479-1484 ◽  
Author(s):  
Gonca Tatar ◽  
Rengin Elsurer ◽  
Halis Simsek ◽  
Yasemin H. Balaban ◽  
Gulsen Hascelik ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Blood Donors ◽  
Tissue Transglutaminase ◽  
Turkish Population ◽  
Disease Screening

scholarly journals Low Prevalence of Celiac Disease among Patients with Functional Gastrointestinal Disorders in Latvia

2020 ◽  
Vol 29 (1) ◽  
pp. 33-39
Author(s):  
Alise Dekante ◽  
Ilva Daugule ◽  
Sergejs Pavlovics ◽  
Ilze Kikuste ◽  
Inese Polaka ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Functional Gastrointestinal Disorders ◽  
Gastrointestinal Disorders ◽  
Mixed Group ◽  
Histopathological Changes ◽  
Irritable Bowel ◽  
Low Prevalence ◽  
Duodenal Biopsies

Background and Aims: Studies suggest that the prevalence of celiac disease (CD) is increased in individuals with functional gastrointestinal disorders (FGIDs), in particular, irritable bowel syndrome (IBS); however, the evidence is conflicting. We aimed to analyze the prevalence of CD in patients with FGIDs in Latvia. Methods: This retrospective study included patients with FGIDs, referred for a gastroenterologist consultation in a secondary gastroenterology practice unit. Patients were divided into three groups – patients only with IBS (IBS group), patients only with functional dyspepsia (FD) (FD group), patients with mixed symptoms IBS and FD (Mixed group). Patient levels of tissue transglutaminase IgA (tTG-IgA) and/or antiendomysial IgA group antibodies (EMA-IgA) were evaluated. Four duodenal biopsies were obtained and reported according to Marsh classification. Patients diagnosed or being referred for confirmation of CD were excluded from the study. Results: Overall, 1,833 FGIDs patients were enrolled. Celiac serology was available for 1,570 patients, duodenal histology for 582 patients, both histology and serology for 319 patients. In total, celiac seropositivity was present in 1.78% (28/1570) (3.18% in IBS group, 0.90% in FD group and 1.11% of cases in the mixed group). Fifteen patients had histopathological changes (2.58%; 15/582). Three IBS patients (2.36%) were both serology and biopsy positive. None of the FD patients had CD. Conclusion: Prevalence of biopsy-proven CD in patients from Latvia with FGIDs was low. Routine screening for CD could be considered only among patients with IBS.

Celiac Disease Could be a Frequent Disease in Mexico: Prevalence of Tissue Transglutaminase Antibody in Healthy Blood Donors

2006 ◽  
Vol 40 (8) ◽  
pp. 697-700 ◽  
Author(s):  
Jos?? Mar??a Remes-Troche ◽  
Mar??a Teresa Ram??rez-Iglesias ◽  
Alberto Rubio-Tapia ◽  
Amada Alonso-Ramos ◽  
Antonio Velazquez ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Blood Donors ◽  
Tissue Transglutaminase

Tissue transglutaminase, the celiac disease autoantigen, preferentially generates antigenic neoepitopes in γ and γ but not in α gliadins

2001 ◽  
Vol 120 (5) ◽  
pp. A684-A684
Author(s):  
D TRAPP ◽  
W DIETERICH ◽  
H WIESER ◽  
M LEIDENBERGER ◽  
D SEILMEIER ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase

245 THE ORAL TISSUE TRANSGLUTAMINASE INHIBITOR ZED1227 PREVENTS GLUTEN-INDUCED ENTEROPATHY IN A TRANSGENIC MOUSE MODEL OF CELIAC DISEASE

2021 ◽  
Vol 160 (6) ◽  
pp. S-53-S-54
Author(s):  
Manuel A. Encalada Ventura ◽  
Martin Hils ◽  
Bernhard Tewes ◽  
Ralf Pasternack ◽  
Roland Greinwald ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Tissue Transglutaminase ◽  
Transgenic Mouse Model ◽  
Oral Tissue
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