scholarly journals Selection of Tobacco Cell Lines Resistant to Photobleaching Herbicides

1999 ◽  
Vol 24 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Mamoru HORIKOSHI ◽  
Takashi HIROOKA
Keyword(s):  
Cell Lines ◽  
Tobacco Cell ◽  
Selection Of

Selection of tobacco cell lines resistant to selenoamino acids

1978 ◽  
Vol 13 (3) ◽  
pp. 219-229 ◽  
Author(s):  
Stuart M. Flashman ◽  
Philip Filner
Keyword(s):  
Cell Lines ◽  
Tobacco Cell ◽  
Selenoamino Acids ◽  
Selection Of

Selection of Tobacco Cell Lines with High Yields of Cinnamoyl Putrescines

Planta Medica ◽  
1981 ◽  
Vol 42 (06) ◽  
pp. 173-180 ◽  
Author(s):  
J. Berlin ◽  
K. Kukoschke ◽  
K.-H. Knobloch
Keyword(s):  
Cell Lines ◽  
Tobacco Cell ◽  
High Yields ◽  
Selection Of

scholarly journals Transgenic human ES and iPS reporter cell lines for identification and selection of pluripotent stem cells in vitro

2014 ◽  
Vol 13 (2) ◽  
pp. 251-261 ◽  
Author(s):  
Dmitry A. Ovchinnikov ◽  
Drew M. Titmarsh ◽  
Patrick R.J. Fortuna ◽  
Alejandro Hidalgo ◽  
Samah Alharbi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Lines ◽  
Pluripotent Stem Cells ◽  
Reporter Cell ◽  
Selection Of

MAGNETIC SELECTION OF TRANSDUCED CELLS USING NOVEL SURFACE MARKERS

2021 ◽  
Vol 1 (19) ◽  
pp. 194-196
Author(s):  
O.F Kandarakov ◽  
A.V. Bruter ◽  
A.V. Petrovskaya ◽  
A.V. Belyavsky
Keyword(s):  
Magnetic Separation ◽  
Cell Lines ◽  
Surface Protein ◽  
Cell Selection ◽  
Surface Markers ◽  
Efficiency Of Selection ◽  
Selection Of

The possibility of using HA- and FLAG–tags embedded into CD52 surface protein for magnetic separation of transduced cells in vitro was investigated. The efficiency of selection of transfected cell lines, both with single and binary tags, was shown to exceed 85%. Thus, surface markers on the basis of CD52 protein with integrated HA- and FLAG-tags are applicable for cell selection by the MACS method.

scholarly journals Synthetic chimeric nucleases function for efficient genome editing

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
R. M. Liu ◽  
L. L. Liang ◽  
E. Freed ◽  
H. Chang ◽  
E. Oh ◽  
...  
Keyword(s):  
Kinetic Parameter ◽  
Genome Editing ◽  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines ◽  
Wild Type ◽  
Functional Variants ◽  
Protospacer Adjacent Motif ◽  
Natural Variants ◽  
Selection Of

AbstractCRISPR–Cas systems have revolutionized genome editing across a broad range of biotechnological endeavors. Many CRISPR-Cas nucleases have been identified and engineered for improved capabilities. Given the modular structure of such enzymes, we hypothesized that engineering chimeric sequences would generate non-natural variants that span the kinetic parameter landscape, and thus provide for the rapid selection of nucleases fit for a particular editing system. Here, we design a chimeric Cas12a-type library with approximately 560 synthetic chimeras, and select several functional variants. We demonstrate that certain nuclease domains can be recombined across distantly related nuclease templates to produce variants that function in bacteria, yeast, and human cell lines. We further characterize selected chimeric nucleases and find that they have different protospacer adjacent motif (PAM) preferences and the M44 chimera has higher specificity relative to wild-type (WT) sequences. This demonstration opens up the possibility of generating nuclease sequences with implications across biotechnology.

Sign in / Sign up

Export Citation Format

Share Document