The Effect of Cadmium on Tobacco Cell Culture and the Selection of Potentially Cd-Resistant Cell lines

1989 ◽  
Vol 31 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Eva DomaZlická ◽  
Z. OpatrnÝ
Keyword(s):  
Cell Culture ◽  
Cell Lines ◽  
Resistant Cell ◽  
Tobacco Cell ◽  
Tobacco Cell Culture ◽  
Selection Of

scholarly journals Cultured Cell Sublines Highly Susceptible to Prion Infection

2000 ◽  
Vol 74 (9) ◽  
pp. 4377-4386 ◽  
Author(s):  
Patrick J. Bosque ◽  
Stanley B. Prusiner
Keyword(s):  
Cell Culture ◽  
Infected Cell ◽  
Tumor Cell ◽  
Cell Lines ◽  
Uninfected Cell ◽  
Resistant Cell ◽  
Cultured Cell ◽  
Prion Infection ◽  
Blot Technique ◽  
Prion Propagation

ABSTRACT Cultured cell lines infected with prions produce an abnormal isoform of the prion protein (PrPSc). In order to derive cell lines producing sufficient quantities of PrPSc for most studies, it has been necessary to subclone infected cultures and select the subclones producing the largest amounts of PrPSc. Since postinfection cloning can introduce differences between infected and uninfected cell lines, we sought an approach to generate prion-infected cell lines that would avoid clonal artifacts. Using an improved cell blot technique, which permits sensitive and rapid comparison of PrPSc levels in multiple independent cell cultures, we discovered marked heterogeneity with regard to prion susceptibility in tumor cell sublines. We exploited this heterogeneity to derive sublines which are highly susceptible to prion infection and used these cells to generate prion-infected lines without further subcloning. These infected sublines can be compared to the cognate uninfected cultures without interference from cloning artifacts. We also used susceptible cell lines and our modified cell blot procedure to develop a sensitive and reproducible quantitative cell culture bioassay for prions. We found that the sublines were at least 100-fold more susceptible to strain RML prions than to strain ME7 prions. Comparisons between scrapie-susceptible and -resistant cell lines may reveal factors that modulate prion propagation.

scholarly journals Selection of Tobacco Cell Lines Resistant to Photobleaching Herbicides

1999 ◽  
Vol 24 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Mamoru HORIKOSHI ◽  
Takashi HIROOKA
Keyword(s):  
Cell Lines ◽  
Tobacco Cell ◽  
Selection Of

Selection of tobacco cell lines resistant to selenoamino acids

1978 ◽  
Vol 13 (3) ◽  
pp. 219-229 ◽  
Author(s):  
Stuart M. Flashman ◽  
Philip Filner
Keyword(s):  
Cell Lines ◽  
Tobacco Cell ◽  
Selenoamino Acids ◽  
Selection Of

scholarly journals Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture

Redox Biology ◽  
2017 ◽  
Vol 11 ◽  
pp. 688-700 ◽  
Author(s):  
Aingeru Calderón ◽  
Ana Ortiz-Espín ◽  
Raquel Iglesias-Fernández ◽  
Pilar Carbonero ◽  
Federico Vicente Pallardó ◽  
...  
Keyword(s):  
Cell Culture ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Tobacco Cell ◽  
Proliferating Cell ◽  
Tobacco Cell Culture
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