scholarly journals Molecular Diversity Analysis of Two in vitro and Irradiated Potato Varieties Expressed by Random Amplified Polymorphic DNA

2018 ◽  
Vol 10 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Ayman El-FIKI ◽  
Zakia ADAM ◽  
Thoria MOHAMED ◽  
Shimaa SOBIEH ◽  
Amal SALAH
Keyword(s):  
Gamma Radiation ◽  
Molecular Diversity ◽  
Solid Medium ◽  
Random Amplified Polymorphic Dna ◽  
Steady Increase ◽  
Radiation Doses ◽  
Survival Percentage ◽  
Polymorphic Bands ◽  
Radiation Treatments

Potato buds cvs. ҅Valor’ and ‘Spunta’ were cultured in vitro on MS solid medium with 0.2 mg -1 BAP. The resulting plantlets were irradiated with gamma radiation doses 10, 20, 30, 40 and 50 Gy. Irradiated segments were transferred onto fresh MS with BAP and plantlets survival percentage was calculated after eight weeks. Gamma radiation caused the death of 3.8% to 81% in cv. ҅Spunta’ and 2.9% to 83.9% in cv. ҅Valor’. Microtubers produced from irradiated plantlets were decreased with increasing gamma radiation doses, with notable changes in shape, size and numbers. The proline contents in irradiated plantlets were steady increase with gamma radiation doses. The genomic DNA of the two cultivars and ten radiation treatments was amplified with 10 RAPD primers that generated 53 polymorphic bands. The highest number of genetic identity was 0.9672 showed between irradiated plantlets with 20 and 30 Gy in cv. ҅Valor’. However, the highest genetic distance was 0.3995 observed between irradiated plantlets with dose 20 Gy in cv. ҅Valor’ and 30 Gy in cv. ҅Spunta’. The dendrogram generated by cluster analysis distinguished the irradiated plantlets genetically.

SOME EFFECTS OF GAMMA RADIATION ON THE STORAGE LIFE OF FRESH STRAWBERRIES

1964 ◽  
Vol 44 (2) ◽  
pp. 188-194 ◽  
Author(s):  
H. B. Heeney ◽  
W. M. Rutherford ◽  
K. F. MacQueen
Keyword(s):  
Gamma Radiation ◽  
Storage Period ◽  
Storage Life ◽  
Radiation Doses ◽  
Apparent Effect ◽  
Fungal Development ◽  
Radiation Treatments ◽  
Storage Temperatures

The effects of gamma radiation doses of 110,000, 220,000, and 330,000 rad on the storage life and quality of two varieties of strawberries stored at 40°, 55°, and 70° F were studied. Results indicated that a dose of 330,000 rad prevented fungal development of the Redcoat variety stored for 26 days at 40° F. The fungal-free period was sharply reduced at lower radiation doses or at higher storage temperatures. Under the conditions of this trial there was no apparent effect of radiation on appearance or texture of fruit.In organoleptic tests in the first two weeks of storage observers were not able to differentiate between radiation treatments. There was some preference for treated berries as the storage period increased. After 20 days the flavor of the fruit deteriorated very rapidly and it soon became commercially unacceptable.

scholarly journals Gamma Radiation as a Potential Phytosanitary Squash Treatment for the Control of Anastrepha grandis (Macquart) (Diptera: Tephritidae)

2021 ◽  
pp. 19-31
Author(s):  
Fernando Berton Baldo ◽  
Anna Lucia Casañas Haasis Villavicencio ◽  
Flávio Luis Schmidt ◽  
Adalton Raga
Keyword(s):  
Gamma Radiation ◽  
Physicochemical Properties ◽  
Fruit Production ◽  
Radiation Doses ◽  
Total Acidity ◽  
Cucurbita Moschata ◽  
Horticultural Crops ◽  
Eggs And Larvae ◽  
Third Instar Larvae

Fruit flies (Diptera: Tephritidae) are the primary pests of horticultural crops worldwide, causing both direct and indirect damage to the fruit production, including Anastrepha grandis (Macquart, 1846), which exclusively infests Cucurbitaceae fruit. Dose-response tests were used to examine the effects of gamma radiation exposure on 24 – 48 h old eggs and third instar larvae, both in vitro and in Atlas squashes (Cucurbita moschata). The following physicochemical properties of Atlas squashes exposed to gamma radiation were evaluated: titratable total acidity, sugar, pH, external colouration, and texture. It was found that under in vitro conditions, a 20.0 Gy dose of gamma radiation was able to prevent larval eclosion, but a 200 Gy dose was necessary to prevent emergence after the exposure of third instar larvae. In Atlas squashes, 200 and 250 Gy doses of gamma radiation were able to prevent the emergence of A. grandis adults from eggs and larvae, respectively. The radiation doses estimated by Probit 9 to prevent emergence from squashes infested with eggs and third instar larvae were 257.13 Gy and 270.25 Gy respectively. The use of gamma radiation did not significantly affect the physicochemical properties of Atlas squashes, which suggested that gamma radiation may represent a potentially useful technique for the quarantine control of A. grandis in cucurbits.

Boosting the Food Functionality (In Vivo and In Vitro) of Spirulina by Gamma Radiation: An Inspiring Approach

2015 ◽  
Vol 11 (4) ◽  
pp. 579-585 ◽  
Author(s):  
Jahid M. M. Islam ◽  
Md. Ismail ◽  
Md. Rakibul Islam ◽  
Md. Faruk Hossain ◽  
Hossain Uddin Shekhar
Keyword(s):  
Radiation Dose ◽  
Gamma Radiation ◽  
Binding Capacity ◽  
Chemical Properties ◽  
Maximum Level ◽  
Biologically Active ◽  
Optimum Dose ◽  
Radiation Doses

Abstract Foods (natural or processed) containing known biologically active compounds, which supplies clinically established and well-documented health benefits, are termed as functional food. Study objectives were to boost food functionality of spirulina, to optimize the required radiation dose, and to test functionality of spirulina both in vitro and in vivo. For this purpose fat binding capacity, sugar binding capacity, hydration property, antioxidative property, total polyphenol content were assessed at different radiation doses. A total of 30 rats were divided into three groups to carry out in vivo experiments to validate the outcomes of in vitro experiments. Targeted physico-chemical properties of spirulina were increased at their maximum level at 15 kGy radiation dose. In vivo experiments validated the outcomes of in vitro experiments. Though gamma radiation improves food functionality of spirulina at various radiation doses, but the optimum dose is recommended as 15 kGy.

scholarly journals The effect of calcium and magnesium on the radiosensitivity of Wolffia arrhiza (L.) Wimm.

2014 ◽  
Vol 53 (3) ◽  
pp. 399-410
Author(s):  
Jan Sarosik ◽  
Hanna Wożakowska-Natkaniec
Keyword(s):  
Gamma Radiation ◽  
Survival Rates ◽  
Acute Radiation ◽  
Basic Medium ◽  
Radiation Doses ◽  
Wolffia Arrhiza ◽  
Calcium And Magnesium

After irradiation with gamma radiation from <sup>60</sup>Co, differences were found in the survival rates of <em>Wolffia arrhiza</em> (L.) Wimm., cultured <em>in vitro</em> with different amounts of calcium and magnesium. Radiation doses ranged from 80 to 3000 mC•kg<sup>-1</sup>. Magnesium and especially calcium increased the resistance of <em>Wolffia</em> to acute radiation. This was expressed by the survival of the plants cultured in mediums supplemented with calcium and magnesium after receiving doses of 1200 and 3000 mC•kg<sup>-1</sup> while plants cultured in basic medium already showed absolute mortality (DL 100) at exposition to 800 mC•kg<sup>-1</sup>. Radiosensitivity of <em>Wolffw</em> arrhiza is high compared with other species from the Lemnaceae family.

scholarly journals Genetic Diversity in Haploid Nicotiana alata Induced by Gamma Irradiation, Salt Tolerance and Detection of These Differences by RAPD

2016 ◽  
Vol 8 (1) ◽  
pp. 73-80 ◽  
Author(s):  
Ayman EL-FIKI ◽  
Abdel-Hadi SAYED ◽  
Gamal EL-METABTEB ◽  
Mohamed ADLY
Keyword(s):  
Gamma Radiation ◽  
Negative Impact ◽  
Random Amplified Polymorphic Dna ◽  
Combined Effect ◽  
Total Soluble Protein ◽  
Polymorphic Band ◽  
Radiation Doses ◽  
Nicotiana Alata ◽  
Ms Medium ◽  
Band Number

Haploid plants of Nicotiana alata were cultured in vitro on MS medium with IAA + KIN. The resulting plantlets were irradiated using gamma radiation doses of 10, 15, 20 and 25 Gy. Single node pieces were cut and transferred onto fresh MS medium. Gamma radiation doses caused the death of 9% and up to 28% of explants. NaCl concentrations caused the death of 8% up to 36% of explants, while the combined effect between gamma radiation doses and salinity had an impact suffused on the percentage of survival. The combined effect of gamma radiation doses 20 Gy and 25 Gy on NaCl concentrations of 100, 150 and 200 mM were deadly. Even more, the combined effect of gamma radiation doses and salinity had a severe negative impact on both the proline content and total soluble protein. Random amplified polymorphic DNA (RAPD) analysis was used to determine the degree of genetic variation in treated haploid Nicotiana alata plants. Total genomic DNAs from different haploid plantlets treated were amplified using five arbitrary primers. Two hundred and seventy bands were detected from plantlets irradiated with doses of 15, 20 and 25 Gy, with polymorphic band number 226 (83.7%). The total number of bands resulted from plant grew on 150 mM and 200 mM NaCl were 260 bands with polymorphic bands 185 (85.6%). However, the total number of bands produced from combined effects between gamma rays and salinity (20 Gy X 50 mM NaCl, 20 Gy X 100 mM NaCl and 25 Gy X 50 mM NaCl) were 270, with polymorphic band number 231 (85.5%). High similarity between treatments was revealed. Treatments relationships were estimated through cluster analysis (UPGMA) based on RAPD data.

scholarly journals Genetic studies on Jatropha Plant (Jatropha Curcas Lam) using diferent Gamma Radiation doses

2015 ◽  
Vol 1 (2) ◽  
Author(s):  
Sharkh  AY - Abo Remalia   S.I.H - Abou El Enin MM - Nabil A
Keyword(s):  
Gamma Radiation ◽  
Low Dose ◽  
Block Design ◽  
The Other ◽  
High Dose ◽  
Radiation Doses ◽  
Genetic Studies ◽  
Survival Percentage ◽  
Randomized Complete Block Design ◽  
Different Doses

The study aimed to compare, the effects of different doses of gamma radiation on germination survival percentage and growth of Jatropha plant, the seeds were treated with three doses of gamma radiation (5, 10 and 15 kr) and arranged in a randomized complete block design with three replications. The results showed significant differences among the three doses for seed germination after 7, 14 and 21 days from sowing in vitroand invivo experiments and on the other handplant height andno. of Leaves /plantwere recorded after 10,20, 30,100,120 and 130 days from sowing invivo experiments andthe low dose 5 kr seemed to have a stimulating effect on these traits, on the other hand the high dose 15 kr caused significant reduction as compared to the control for all studied traits.  

EVALUATION OF GAMMA RADIATION DOSES IN SOIL USING THE MONTE CARLO SIMULATION

2012 ◽  
Vol 11 (7) ◽  
pp. 1355-1360
Author(s):  
Clement Ionescu ◽  
Liana Simona Sbirna ◽  
Monica Daniela Mateescu ◽  
Clementina Sabina Moldovan ◽  
Sebastian Sbirna
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Gamma Radiation ◽  
Radiation Doses

Crystal growth and thermoluminescence response of NaZr2(PO4)3 at high gamma radiation doses

2013 ◽  
Vol 443 (1-3) ◽  
pp. 417-423 ◽  
Author(s):  
E. Ordóñez-Regil ◽  
A. Contreras-Ramírez ◽  
S.M. Fernández-Valverde ◽  
P.R. González-Martínez ◽  
H. Carrasco-Ábrego
Keyword(s):  
Crystal Growth ◽  
Gamma Radiation ◽  
Radiation Doses ◽  
High Gamma
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