scholarly journals Antioxidant, anti-cancer and ameliorative activities of Spirulina platensis and pomegranate juice against hepatic damage induced by CCl4

2020 ◽  
Vol 48 (4) ◽  
pp. 1941-1956
Author(s):  
Hossam S. EL-BELTAGI ◽  
Faten DHAWI ◽  
Ihab S. ASHOUSH ◽  
Khaled RAMADAN
Keyword(s):  
Lipid Peroxidation ◽  
Spirulina Platensis ◽  
Antioxidant Status ◽  
Liver Homogenate ◽  
Water Extract ◽  
Pomegranate Juice ◽  
Total Phenolic ◽  
Liver Carcinoma ◽  
Marker Enzymes ◽  
Liver Marker Enzymes

Due to the excessive impact of synthetic drugs, unravelling and employing safe, natural alternatives are now needed to resolve a number of diseases. In this research, we have evaluated hepatoprotective and antioxidant activities of Spirulina platensis and pomegranate juice in rats against hepatotoxicity induced by carbon tetrachloride (CCl4). Spirulina crude carotenoid extract was screened by UPLC-MS / MS. Activities of liver marker enzymes; measured aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and determined lipid peroxidation and antioxidant status as reduced glutathione (GSH) in liver homogenate. The infusion of CCl4 (2 ml/kg b.wt) greatly increases levels of liver marker enzymes and lipid peroxidation, resulting in depletion of antioxidants. Treatment of Spirulina platensis (Sp), pomegranate juice (Pj)or mixture (PJSP) of Spirulina water extract 10% and pomegranate juice 90% (1 ml/100 g b.wt) to CCl4-disrupted rats resulted in decreased activity of liver marker enzymes, lipid peroxidation with increased antioxidant status. Chromatographic separation showed that ß-carotene is the predominant carotenoid extract. This carotenoid extract was tested for colon carcinoma (HCT-116), liver carcinoma (HepG2) and intestinal carcinoma cell lines (CACO) LC50 for 21.8, 14 and 11.3 ug / ml, respectively. Total phenolic phytochemicals, total carotenoids and total flavonoids were also measured in Spirulina. Our study clearly demonstrates that Spirulina platensis and pomegranate juice had hepatoprotective effect on CCl4-caused hepatotoxicity in rats through its antioxidant activity.

scholarly journals Antioxidant activities of aqueous extracts from nine different rose cultivars

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Hamadia Khurshid ◽  
Syed Mubashar Sabir ◽  
Shahid Iqbal Awan ◽  
Syed Rizwan Abbas ◽  
Muhammad Irshad
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Dpph Radical ◽  
Rose Petals

Rose petals have been applied as food additives in teas, cakes and flavor extracts. The aim of this research study was to explore and reveal the antioxidant potential of aqueous extracts of rose petals belonging to nine genotypes of rose (wild as well as hybrid). The in vitro antioxidant activities of roses were studied by lipid peroxidation assay, DPPH radical scavenging assay, iron chelation assay, phosphomolybdenum reduction assay and total phenolic and flavonoid contents. The aqueous extract showed inhibition against lipid peroxidation (TBARS), induced by prooxidants (10 µM FeSO4) in mice liver homogenate. The free radical scavenging activities of the extracts were determined by scavenging of the DPPH radical. Extracts also showed metal chelating activities and high antioxidant activity in the phosphomolybdenum assay. The high content of phenolics and flavonoids detected in aqueous extracts may be responsible for the antioxidant activity. Amongst the different rose genotypes, screened, Rosa moschata (musk rose) was found to carry slightly higher antioxidant potential, owing to its higher phytochemical content.

scholarly journals Comparing Different Solvent Extracts of Rhus semialata var. roxburghiana Stem against Ferrous Ion-Induced Lipid Peroxidation in Mice Liver Mitochondria

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800
Author(s):  
Pei-Chin Lin ◽  
Wei-Fung Bi ◽  
Che-Hsuan Lin ◽  
Fei-Peng Lee ◽  
Ling-Ling Yang
Keyword(s):  
Lipid Peroxidation ◽  
Gallic Acid ◽  
Radical Scavenging ◽  
Water Extract ◽  
Liver Mitochondria ◽  
Ferrous Ion ◽  
Main Peak ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Mice Liver

Rhus semialata Murr. var. roxburghiana DC. stem ( RSRS) is a Taiwanese native folk medicine for anti-inflammation and the treatment of diarrhea and liver disease. Five extracts and a main constituent, gallic acid, elucidated inhibitory effects on ferrous ion-induced lipid peroxidation (LPO) in mice liver mitochondria (IC50: 10.8 ± 0.7 −55.2 ± 0.6 and gallic acid: 36.1 ± 3.1 μg/mL). None of the samples tested induced LPO production. The IC50 values for antioxidant activities were 1) 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging: 1.5 ± 0.08 – 56.2 ± 1.1 μg/mL; 2) superoxide anion radical scavenging: 53.5 ± 0.7 – 226.6 ± 2.1 μg/mL; 3) hydrogen peroxide scavenging: ethyl acetate (EA) and acetone extracts 264.2 ± 4.7 and 208.0 ± 1.9 μg/mL, respectively; and 4) ferric-reducing antioxidant power (FRAP): EA extract, equivalent Trolox 116.4 ± 0.3 μM. The total phenolic content of the extracts was 82.0 ± 2.1 – 455.2 ± 9.3 μg gallic acid equivalents/mg. The highest flavonoid and flavanol contents of the extracts were acetone extract 30.9 ± 2.7 μg rutin equivalents/mg and water extract 10.9 ± 0.05 μg epicatechin equivalents/mg. Next, the gallic acid content of each extract was quantitatively analyzed using high-performance liquid chromatography (HPLC), which showed one main peak. Based on this evidence, RSRS and gallic acid may be used as a potential antioxidant and LPO inhibitor for developing a natural liver-protective phytochemical.

scholarly journals Influence of Hibiscus sabdariffa (Gongura) on the levels of circulatory lipid peroxidation products and liver marker enzymes in experimental hyperammonemia

2006 ◽  
Vol 4 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Musthafa Mohamed Essa ◽  
Perumal Subramanian ◽  
Ganapathy Suthakar ◽  
Tamilarasan Manivasagam ◽  
Kadiyala Babu Dakshayani ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Hibiscus Sabdariffa ◽  
Marker Enzymes ◽  
Liver Marker Enzymes ◽  
Lipid Peroxidation Products

scholarly journals Effect ofWithania SomniferaRoot Powder on the Levels of Circulatory Lipid Peroxidation and Liver Marker Enzymes in Chronic Hyperammonemia

2008 ◽  
Vol 5 (4) ◽  
pp. 872-877 ◽  
Author(s):  
B. Harikrishnan ◽  
P. Subramanian ◽  
S. Subash
Keyword(s):  
Lipid Peroxidation ◽  
Ammonium Chloride ◽  
Radical Scavenging ◽  
Thiobarbituric Acid ◽  
Underlying Mechanism ◽  
Antioxidant Property ◽  
Marker Enzymes ◽  
Liver Marker Enzymes ◽  
Lipid Peroxidation Products ◽  
Liver Markers

Withania somnifera(L) Dunal (Solanaceae), commonly called Ashwagandha (Sanskrit) is an Ayurvedic Indian medicinal plant, which has been widely used as a home remedy for several ailments. We have investigated the influence ofW.somniferaroot powder on the levels of circulatory ammonia, urea, lipid peroxidation products such as TBARS (thiobarbituric acid and reactive substances), HP (hydroperoxides) and liver marker enzymes such as AST (aspartate transaminase), ALT (alanine transaminase) and ALP (alkaline phosphatase), for its hepatoprotective effect in ammonium chloride induced hyperammonemia. Ammonium chloride treated rats showed a significant increase in the levels of circulatory ammonia, urea, AST, ALT, ALP, TBARS and HP. These changes were significantly decreased in rats treated withW.somniferaroot powder and ammonium chloride. Our results indicate thatW.somniferaoffers hepatoprotection by influencing the levels of lipid peroxidation products and liver markers in experimental hyperammonemia and this could be due to (i) the presence of alkaloids, withanolids and flavonoids, (ii) normalizing the levels of urea and urea related compounds, (iii) its free radical scavenging property and (iv) its antioxidant property. The exact underlying mechanism is still unclear and further research needed.

scholarly journals Phenolic compounds profile, neuroprotective effect and antioxidant potential of a commercial Turkish coffee

2020 ◽  
Vol 33 ◽  
Author(s):  
Melek ÇOL AYVAZ
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Neuroprotective Effect ◽  
Radical Scavenging ◽  
Water Extract ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity

ABSTRACT Objective The purpose of this study is to determine the phenolic and flavonoid contents, and antioxidant activities and neuroprotective effects of powdered coffee sample of a commercial coffee brand originated from Sivas, Turkey. Methods Total phenolic, flavonoid and antioxidant contents, enzymatic and non-enzymatic antioxidative activities based on 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity, metal chelating potential, reducing power, superoxide dismutase and catalase activity tests and lipid peroxidation inhibition potentials of the ethanolic and aqueous extracts of the coffee sample were assayed using the commonly preferred spectrophotometric methods. Furthermore the extracts’ cholinesterase and tyrosinase inhibition potentials were evaluated. Phenolic profiles of the coffee sample were investigated using high performance liquid chromatography. Results Catechin was the most frequently detected phenolic acid. In addition, it was demonstrated that the water extract has a significant impact when compared with standard antioxidants. While the SC50 (sufficient concentration to obtain 50% of a maximum scavenging capacity) value for the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl free radical was calculated as being 0.08mg/mL for water extract, the amount of chelating agents with half Fe2+ ions in the medium was found to be 0.271mg/mL. Additionally, it was shown that 0.1mg/mL concentration of both extracts prevents lipid peroxidation by 8%. Compared with standard drugs, inhibition potentials of cholinesterase and tyrosinase enzymes were considered as moderately acceptable in these samples. Conclusion Besides the extracts’ enzymatic antioxidant activity, their inhibition potential on cholinesterase and tyrosinase enzymes – which are important clinical enzymes – reveal that this natural source can be used as a valuable resource in different fields, especially in medicine.

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